##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1033092_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4618884 Sequences flagged as poor quality 0 Sequence length 100 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.71607470549163 34.0 31.0 34.0 31.0 34.0 2 32.71813061336894 34.0 31.0 34.0 31.0 34.0 3 32.82001561416134 34.0 31.0 34.0 31.0 34.0 4 36.302929885227684 37.0 37.0 37.0 35.0 37.0 5 36.28551767050222 37.0 37.0 37.0 35.0 37.0 6 36.34834886522372 37.0 37.0 37.0 35.0 37.0 7 36.29990166455793 37.0 37.0 37.0 35.0 37.0 8 36.309377546610826 37.0 37.0 37.0 35.0 37.0 9 38.11786180384699 39.0 39.0 39.0 37.0 39.0 10-11 38.07766291597711 39.0 39.0 39.0 36.0 39.0 12-13 38.04972651402373 39.0 39.0 39.0 35.0 39.0 14-15 39.54413501183403 41.0 39.5 41.0 37.0 41.0 16-17 39.545035554042926 41.0 40.0 41.0 37.0 41.0 18-19 39.55798521894033 41.0 40.0 41.0 37.0 41.0 20-21 39.54818057781923 41.0 40.0 41.0 37.0 41.0 22-23 39.47661664592572 41.0 39.0 41.0 36.5 41.0 24-25 39.41843148691329 41.0 39.0 41.0 36.0 41.0 26-27 39.308445070281046 41.0 39.0 41.0 36.0 41.0 28-29 39.25553098973691 41.0 39.0 41.0 36.0 41.0 30-31 39.25415771428769 41.0 39.0 41.0 36.0 41.0 32-33 39.221017566148014 41.0 39.0 41.0 35.0 41.0 34-35 39.13991821401014 41.0 39.0 41.0 35.0 41.0 36-37 39.052605239707255 41.0 39.0 41.0 35.0 41.0 38-39 38.96362368052543 41.0 39.0 41.0 35.0 41.0 40-41 38.86142507584083 41.0 39.0 41.0 35.0 41.0 42-43 38.7400584210385 41.0 39.0 41.0 35.0 41.0 44-45 38.572657486094045 41.0 38.0 41.0 35.0 41.0 46-47 38.37325996929128 40.5 38.0 41.0 34.5 41.0 48-49 38.1695679086117 40.0 38.0 41.0 34.0 41.0 50-51 38.02452627084811 40.0 38.0 41.0 34.0 41.0 52-53 37.88044179070096 40.0 37.5 41.0 33.5 41.0 54-55 37.720261322865 40.0 37.0 41.0 33.0 41.0 56-57 37.557406940724206 40.0 37.0 41.0 33.0 41.0 58-59 37.3678746857466 40.0 36.0 41.0 33.0 41.0 60-61 37.16519444956833 39.5 36.0 41.0 33.0 41.0 62-63 36.92743777501232 39.0 35.0 41.0 32.5 41.0 64-65 36.65318830262895 39.0 35.0 41.0 32.0 41.0 66-67 36.370929103220604 38.5 35.0 41.0 32.0 41.0 68-69 36.06183560790875 37.5 35.0 40.0 31.5 41.0 70-71 35.73922196357388 37.0 35.0 40.0 31.0 41.0 72-73 35.395595126441805 36.5 35.0 39.0 31.0 41.0 74-75 35.05356596961517 36.0 35.0 39.0 31.0 41.0 76-77 34.707727126292845 35.5 35.0 38.5 31.0 40.0 78-79 34.35980704429902 35.0 34.5 37.0 30.0 39.0 80-81 34.045324476648474 35.0 34.0 37.0 30.0 39.0 82-83 33.758217028182564 35.0 34.0 36.5 30.0 39.0 84-85 33.547588984698464 35.0 34.0 36.0 30.0 37.0 86-87 33.352467284305035 35.0 34.0 36.0 30.0 37.0 88-89 33.15749659441545 35.0 34.0 35.0 30.0 37.0 90-91 32.987656542143085 35.0 34.0 35.0 30.0 36.0 92-93 32.8230266228812 35.0 34.0 35.0 29.5 36.0 94-95 32.67343486002247 35.0 34.0 35.0 29.0 36.0 96-97 32.53636246331365 35.0 34.0 35.0 29.0 36.0 98-99 32.41579524404597 35.0 34.0 35.0 29.0 35.0 100 32.317348952690736 35.0 34.0 35.0 29.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.1250809536567985 1101 2 0.07506467073991274 1101 3 0.1174081200709125 1101 4 0.11630115386795836 1101 5 0.07211829956029447 1101 6 0.09925349139093242 1101 7 0.15233250149135102 1101 8 0.14286742426754984 1101 9 0.18776226100668225 1101 10-11 0.19930885449174696 1101 12-13 0.19759255805579556 1101 14-15 0.2849391019466907 1101 16-17 0.327152399223678 1101 18-19 0.26524268328185485 1101 20-21 0.23239357324161602 1101 22-23 0.28245789249286446 1101 24-25 0.32304214312011226 1101 26-27 0.3161057970754584 1101 28-29 0.33173825141321345 1101 30-31 0.33488930185470167 1101 32-33 0.3077406406873493 1101 34-35 0.30199589901212676 1101 36-37 0.3467594179805715 1101 38-39 0.32210564330738833 1101 40-41 0.3375399135096657 1101 42-43 0.3216091472227234 1101 44-45 0.3476327337743257 1101 46-47 0.3424596384276981 1101 48-49 0.3041264796520835 1101 50-51 0.35988688884610553 1101 52-53 0.3810416993261754 1101 54-55 0.3883860927493359 1101 56-57 0.3722182189074843 1101 58-59 0.35303488814045636 1101 60-61 0.352101699633522 1101 62-63 0.3807256726025514 1101 64-65 0.3377225807210209 1101 66-67 0.37972669337899134 1101 68-69 0.3460725245258658 1101 70-71 0.38793830449350963 1101 72-73 0.3798127274347465 1101 74-75 0.3015213952790603 1101 76-77 0.31432401180099845 1101 78-79 0.3327138221336412 1101 80-81 0.33661331928083627 1101 82-83 0.3474874906455767 1101 84-85 0.3467791849985602 1101 86-87 0.3564125665239999 1101 88-89 0.3929487212659737 1101 90-91 0.40265250832693056 1101 92-93 0.31567468943387667 1101 94-95 0.3474482483116006 1101 96-97 0.3695683897313202 1101 98-99 0.31765165669717277 1101 100 0.2683769511045284 1102 1 0.1525759932115207 1102 2 0.10951635900920564 1102 3 0.1498296763193494 1102 4 0.10443602644249239 1102 5 0.12034563827066336 1102 6 0.11645635857124859 1102 7 0.15107757325674953 1102 8 0.16981230534570813 1102 9 0.21641989150289476 1102 10-11 0.23245397006368052 1102 12-13 0.2229997010969953 1102 14-15 0.2856134862931512 1102 16-17 0.290763989916087 1102 18-19 0.2684836149007168 1102 20-21 0.2744188521861375 1102 22-23 0.27532580292139386 1102 24-25 0.3042249838447262 1102 26-27 0.3203016680952899 1102 28-29 0.34648602028652675 1102 30-31 0.33632890467549714 1102 32-33 0.2796390739597001 1102 34-35 0.26291134811495454 1102 36-37 0.23525719759077646 1102 38-39 0.26104481665240087 1102 40-41 0.30762552950677957 1102 42-43 0.23439796968602877 1102 44-45 0.20551586911931707 1102 46-47 0.23938491393641215 1102 48-49 0.22660803366724735 1102 50-51 0.2406723571035485 1102 52-53 0.23788765065797435 1102 54-55 0.24595936131772334 1102 56-57 0.20299617133874648 1102 58-59 0.24113003420517032 1102 60-61 0.25002862003448456 1102 62-63 0.23935749160427378 1102 64-65 0.2650303688348572 1102 66-67 0.28102575025016563 1102 68-69 0.27188455586041727 1102 70-71 0.29100014471134017 1102 72-73 0.29327593424451237 1102 74-75 0.342013553051288 1102 76-77 0.2713826610137673 1102 78-79 0.2893713409661558 1102 80-81 0.2337080229854891 1102 82-83 0.21509513875029995 1102 84-85 0.23292236363472085 1102 86-87 0.2637809751444351 1102 88-89 0.29357564853854257 1102 90-91 0.21694932762928687 1102 92-93 0.2961572698961561 1102 94-95 0.29136526070627156 1102 96-97 0.31602231556944105 1102 98-99 0.2539111159012677 1102 100 0.26899238989993535 1103 1 0.07705623112532578 1103 2 0.08416455314772264 1103 3 0.1357370067431347 1103 4 0.07134201897918047 1103 5 0.10473106337320104 1103 6 0.12237457273822372 1103 7 0.1417316492349272 1103 8 0.13413320452354327 1103 9 0.1884065138580553 1103 10-11 0.1848474152343229 1103 12-13 0.1805389257927601 1103 14-15 0.22030287864882325 1103 16-17 0.24705400315190218 1103 18-19 0.23441666598422728 1103 20-21 0.275259616749679 1103 22-23 0.22759418517741636 1103 24-25 0.2501888005073951 1103 26-27 0.25490279351847533 1103 28-29 0.3086466594111599 1103 30-31 0.2457502368325919 1103 32-33 0.2231682635826644 1103 34-35 0.3073243667556653 1103 36-37 0.33530478331456237 1103 38-39 0.32689036456591225 1103 40-41 0.33303648225935234 1103 42-43 0.31266238507718214 1103 44-45 0.37311034117501407 1103 46-47 0.41684354457515127 1103 48-49 0.37475523527213994 1103 50-51 0.3534631156080792 1103 52-53 0.3483840819713606 1103 54-55 0.36222543072722857 1103 56-57 0.361136694236599 1103 58-59 0.3123545646419501 1103 60-61 0.3460836820479045 1103 62-63 0.3390002576648996 1103 64-65 0.31535444935166623 1103 66-67 0.2654315082938936 1103 68-69 0.23303799916737233 1103 70-71 0.3123338446506665 1103 72-73 0.35796678311313457 1103 74-75 0.32772161554268564 1103 76-77 0.3637399375603607 1103 78-79 0.29840469710508444 1103 80-81 0.3136823340001982 1103 82-83 0.3117853625087008 1103 84-85 0.3411767050241892 1103 86-87 0.3100293610256486 1103 88-89 0.3352172566432827 1103 90-91 0.30742409567537976 1103 92-93 0.33493056669558996 1103 94-95 0.336010882063583 1103 96-97 0.3760502156343577 1103 98-99 0.35232840078131034 1103 100 0.33877854355525727 1104 1 0.08803856772338037 1104 2 0.03266726492269356 1104 3 0.10706788739447859 1104 4 0.07180724388260984 1104 5 0.08080054262685366 1104 6 0.09860532641584996 1104 7 0.12421251749178452 1104 8 0.1251353138442184 1104 9 0.15486090228923644 1104 10-11 0.18089393347360527 1104 12-13 0.18174303730751262 1104 14-15 0.2342585629877476 1104 16-17 0.2182884379780461 1104 18-19 0.2336270323959937 1104 20-21 0.22325087339095973 1104 22-23 0.2008110732399473 1104 24-25 0.21512768759696854 1104 26-27 0.24576886905814632 1104 28-29 0.23197794072449796 1104 30-31 0.19161835686433193 1104 32-33 0.2126083756404853 1104 34-35 0.1930975713422356 1104 36-37 0.22252932487141663 1104 38-39 0.21921192185512695 1104 40-41 0.20585592318708734 1104 42-43 0.23915313066186172 1104 44-45 0.24213691053856223 1104 46-47 0.2164823908280198 1104 48-49 0.1453378504206171 1104 50-51 0.15975579388208416 1104 52-53 0.18317570452225596 1104 54-55 0.2124470568569805 1104 56-57 0.22765181962547132 1104 58-59 0.16134577585006582 1104 60-61 0.2065968482315057 1104 62-63 0.19773475559264142 1104 64-65 0.18825559025529515 1104 66-67 0.12873761804392103 1104 68-69 0.15054427146715454 1104 70-71 0.17725203441300863 1104 72-73 0.1519153256785657 1104 74-75 0.14883387237233592 1104 76-77 0.16298755384732289 1104 78-79 0.14338795990188657 1104 80-81 0.15845981913413 1104 82-83 0.1359021857692042 1104 84-85 0.17192809770987338 1104 86-87 0.1796242433923041 1104 88-89 0.208655890396507 1104 90-91 0.24105302793507377 1104 92-93 0.2166216950878379 1104 94-95 0.24296483861407125 1104 96-97 0.2580328070300695 1104 98-99 0.27858945651504285 1104 100 0.2941026055133307 1105 1 0.01853317521734965 1105 2 0.07077042637424569 1105 3 0.0891119493003103 1105 4 0.0543666356490462 1105 5 0.0595416130567159 1105 6 0.1036985109147679 1105 7 0.08739097307687871 1105 8 0.060983823888662414 1105 9 0.08525094038016334 1105 10-11 0.11703028115046976 1105 12-13 0.10691453945519669 1105 14-15 0.18479087467886757 1105 16-17 0.1852816182303414 1105 18-19 0.16369451516505507 1105 20-21 0.1300585566353618 1105 22-23 0.12427651667204032 1105 24-25 0.10966262136413008 1105 26-27 0.1446781391762002 1105 28-29 0.1858314122760234 1105 30-31 0.14781025330690056 1105 32-33 0.18478180060694172 1105 34-35 0.18380740908179405 1105 36-37 0.17799330632102794 1105 38-39 0.18188089939535246 1105 40-41 0.1806640067604519 1105 42-43 0.22655065565373889 1105 44-45 0.2704163189657862 1105 46-47 0.2179549782862935 1105 48-49 0.2532501552486863 1105 50-51 0.2487657971100674 1105 52-53 0.269735011263883 1105 54-55 0.2911129349240156 1105 56-57 0.28236761520734177 1105 58-59 0.28587339785441657 1105 60-61 0.24605890306907696 1105 62-63 0.26085087893170567 1105 64-65 0.29192380210971436 1105 66-67 0.26049176989562994 1105 68-69 0.30016673451999054 1105 70-71 0.2885307639370893 1105 72-73 0.2926325626112245 1105 74-75 0.2860405634078518 1105 76-77 0.320492204537409 1105 78-79 0.3029374269888194 1105 80-81 0.32822881165314755 1105 82-83 0.3253200952489692 1105 84-85 0.2926314193485169 1105 86-87 0.26576402351761175 1105 88-89 0.2598737573361092 1105 90-91 0.27153107687217215 1105 92-93 0.32635444659494794 1105 94-95 0.30403054816731867 1105 96-97 0.29713651332497903 1105 98-99 0.2802544715664581 1105 100 0.333762511860229 1106 1 0.038748537803826366 1106 2 0.05411260892716996 1106 3 0.059826607332396975 1106 4 0.04697702245584878 1106 5 0.06212365529538744 1106 6 0.058575036941796554 1106 7 0.06159777792669274 1106 8 0.055877018103323906 1106 9 0.05539841580195315 1106 10-11 0.09316874148863974 1106 12-13 0.05935493387150359 1106 14-15 0.10430601628785041 1106 16-17 0.13795681998107767 1106 18-19 0.12189287436440566 1106 20-21 0.1129063243461772 1106 22-23 0.1231109967853925 1106 24-25 0.10587536572305822 1106 26-27 0.1412663722794676 1106 28-29 0.12945124115305617 1106 30-31 0.1374240996054823 1106 32-33 0.09710623788571127 1106 34-35 0.08645446131201595 1106 36-37 0.05551350257600518 1106 38-39 0.055452043149330166 1106 40-41 0.1076669693661998 1106 42-43 0.12397506499039679 1106 44-45 0.06603392094612559 1106 46-47 0.05697389843556522 1106 48-49 0.13593865024532192 1106 50-51 0.12259213552952275 1106 52-53 0.07161461286543869 1106 54-55 0.14951817699223824 1106 56-57 0.0894489648945509 1106 58-59 0.09877005720929333 1106 60-61 0.1010343297159011 1106 62-63 0.07423387762744937 1106 64-65 0.08644785765999075 1106 66-67 0.07942488850594742 1106 68-69 0.08920953255850606 1106 70-71 0.06810700046401763 1106 72-73 0.07572660663338127 1106 74-75 0.12956184027963502 1106 76-77 0.12252579226815641 1106 78-79 0.11545312567501753 1106 80-81 0.14298550606717697 1106 82-83 0.12542919835996713 1106 84-85 0.17230318508379128 1106 86-87 0.12387744432995618 1106 88-89 0.1622119106110631 1106 90-91 0.12369788389663228 1106 92-93 0.19167668645480518 1106 94-95 0.18918165182797964 1106 96-97 0.18871986405962815 1106 98-99 0.25535271467276033 1106 100 0.17485685789731065 1107 1 1.7245709275925947E-4 1107 2 0.02669688261475045 1107 3 0.015613320036258926 1107 4 0.022245902451260235 1107 5 0.017132683091290346 1107 6 0.050107150624043584 1107 7 0.057178851872642156 1107 8 5.015476003791264E-4 1107 9 0.05200776341646929 1107 10-11 0.055759787231842495 1107 12-13 0.034282699101972014 1107 14-15 0.11499207874741302 1107 16-17 0.0646044699857029 1107 18-19 0.11035753020814809 1107 20-21 0.07542299855720103 1107 22-23 0.11729993781002435 1107 24-25 0.10626474420701726 1107 26-27 0.10283541905798899 1107 28-29 0.13248323692973685 1107 30-31 0.0862441357587116 1107 32-33 0.09592550047409532 1107 34-35 0.08018815522746081 1107 36-37 0.033856493487917305 1107 38-39 0.012330018299657297 1107 40-41 0.005810258209891117 1107 42-43 0.017316029708993597 1107 44-45 0.048715675154056726 1107 46-47 0.019526223895475425 1107 48-49 -0.0022731802264388534 1107 50-51 0.04069503672136676 1107 52-53 0.05178607561305171 1107 54-55 0.07495619651827923 1107 56-57 0.03311576647261205 1107 58-59 0.041901406878551484 1107 60-61 0.0572401232708728 1107 62-63 0.034946075227182405 1107 64-65 -0.06450182140099514 1107 66-67 -0.02599838746292704 1107 68-69 -0.015722256470354523 1107 70-71 0.04958623972859044 1107 72-73 0.036969021966818616 1107 74-75 0.03717816624774173 1107 76-77 0.016160196614613653 1107 78-79 -0.00490757163146327 1107 80-81 0.010692014139600303 1107 82-83 -0.0039172953291952695 1107 84-85 0.004613069018375882 1107 86-87 0.027580131328427626 1107 88-89 0.023693649667215766 1107 90-91 -0.007080482505124053 1107 92-93 0.051465621812241125 1107 94-95 0.022957157343142853 1107 96-97 0.016224754229604343 1107 98-99 0.013127472184358169 1107 100 0.0023262965158394877 1108 1 -0.017083866772196643 1108 2 9.936652432784854E-4 1108 3 0.01343320745782961 1108 4 0.011213168470789014 1108 5 0.01578468818475187 1108 6 0.04483051625201995 1108 7 0.031840525752087956 1108 8 0.028797709235874436 1108 9 0.04984681077468167 1108 10-11 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-0.24261247321972945 2214 52-53 -0.28818132685752573 2214 54-55 -0.2480003121736729 2214 56-57 -0.2155171268048406 2214 58-59 -0.20974447302947397 2214 60-61 -0.21266565637533574 2214 62-63 -0.2665662968662019 2214 64-65 -0.2260971130865741 2214 66-67 -0.27388346405276565 2214 68-69 -0.33216800105189037 2214 70-71 -0.24022785875844477 2214 72-73 -0.23643986529768313 2214 74-75 -0.25719705497687073 2214 76-77 -0.24432171595990582 2214 78-79 -0.20323863757251104 2214 80-81 -0.23403821948036807 2214 82-83 -0.2016470886658368 2214 84-85 -0.20863493626412577 2214 86-87 -0.249174476893117 2214 88-89 -0.2017475160823281 2214 90-91 -0.21733400906447997 2214 92-93 -0.24130968977819123 2214 94-95 -0.29004107279046565 2214 96-97 -0.23298581667457796 2214 98-99 -0.23140229093542786 2214 100 -0.23300332645082733 2215 1 -0.17667271462148904 2215 2 -0.13326194856327334 2215 3 -0.14399405800466525 2215 4 -0.1018933348817157 2215 5 -0.13600765928510583 2215 6 -0.17229326126732047 2215 7 -0.19697090975593312 2215 8 -0.15511310205521767 2215 9 -0.25184161251424797 2215 10-11 -0.1846632671391788 2215 12-13 -0.19630401806180942 2215 14-15 -0.2786809386728706 2215 16-17 -0.27731899861790055 2215 18-19 -0.2743220467283365 2215 20-21 -0.3206484186297942 2215 22-23 -0.3284612751337832 2215 24-25 -0.34376402328435773 2215 26-27 -0.3474601250813052 2215 28-29 -0.3444378575326823 2215 30-31 -0.38534795169229596 2215 32-33 -0.29383056910938876 2215 34-35 -0.2220927250159619 2215 36-37 -0.25169105470535413 2215 38-39 -0.2681010012816216 2215 40-41 -0.2788224639511654 2215 42-43 -0.29943615902674026 2215 44-45 -0.302830154082784 2215 46-47 -0.3075017835219924 2215 48-49 -0.2784562569580089 2215 50-51 -0.2606586088799645 2215 52-53 -0.2570127443738599 2215 54-55 -0.31801975496943413 2215 56-57 -0.3143135276008948 2215 58-59 -0.31058377782530044 2215 60-61 -0.3079824486777767 2215 62-63 -0.27059848948258747 2215 64-65 -0.2586846397016842 2215 66-67 -0.33249751792070725 2215 68-69 -0.2691919822639477 2215 70-71 -0.27730201423899103 2215 72-73 -0.27595437423347136 2215 74-75 -0.22688225728333578 2215 76-77 -0.25033855261275306 2215 78-79 -0.27297397858406214 2215 80-81 -0.2405537688163477 2215 82-83 -0.22683648191281947 2215 84-85 -0.2240384551829706 2215 86-87 -0.21989286712175016 2215 88-89 -0.23912361906165103 2215 90-91 -0.20394896920167582 2215 92-93 -0.25049309164481315 2215 94-95 -0.2996340441195784 2215 96-97 -0.24273215872630516 2215 98-99 -0.25134162290311934 2215 100 -0.25312553965116535 2216 1 -0.1659599675984964 2216 2 -0.1271097984721763 2216 3 -0.13634496442225696 2216 4 -0.12914437486424646 2216 5 -0.1579682162997571 2216 6 -0.16043800677431364 2216 7 -0.14570236969331063 2216 8 -0.13889739724260863 2216 9 -0.15708092407341212 2216 10-11 -0.20929171405283853 2216 12-13 -0.26208612947501564 2216 14-15 -0.2949539883384631 2216 16-17 -0.30835302438254786 2216 18-19 -0.29966132699065895 2216 20-21 -0.3419038394228693 2216 22-23 -0.3261198025982779 2216 24-25 -0.3455975175537489 2216 26-27 -0.33354475207499235 2216 28-29 -0.36157546958279596 2216 30-31 -0.34883403683296876 2216 32-33 -0.3262228927069515 2216 34-35 -0.35634407721973105 2216 36-37 -0.35588875398129005 2216 38-39 -0.3231750821458945 2216 40-41 -0.2731080119928393 2216 42-43 -0.28050871511565845 2216 44-45 -0.3317070233825632 2216 46-47 -0.2532147467556243 2216 48-49 -0.3204556179458251 2216 50-51 -0.28978222915952756 2216 52-53 -0.244053461367578 2216 54-55 -0.21430290255930373 2216 56-57 -0.30096805409444727 2216 58-59 -0.24835998925632907 2216 60-61 -0.24489498498178364 2216 62-63 -0.23544184510538457 2216 64-65 -0.3015720053510549 2216 66-67 -0.31126404507624983 2216 68-69 -0.24609648281690255 2216 70-71 -0.2524569364026874 2216 72-73 -0.24619108799608114 2216 74-75 -0.3316358934280004 2216 76-77 -0.36152149007202183 2216 78-79 -0.39086476951435145 2216 80-81 -0.3465239750831657 2216 82-83 -0.29569812400603723 2216 84-85 -0.3959299321967933 2216 86-87 -0.37424564499888646 2216 88-89 -0.5587266114916574 2216 90-91 -0.4918329730790916 2216 92-93 -0.4720249445158444 2216 94-95 -0.40819952526391035 2216 96-97 -0.554465363945873 2216 98-99 -0.5951763180521823 2216 100 -0.5904497260757324 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 23.0 3 6.0 4 16.0 5 37.0 6 116.0 7 240.0 8 559.0 9 1124.0 10 1874.0 11 2881.0 12 4592.0 13 7170.0 14 8461.0 15 11041.0 16 13442.0 17 19388.0 18 22609.0 19 12123.0 20 8590.0 21 8131.0 22 8624.0 23 9437.0 24 11027.0 25 13340.0 26 17212.0 27 22330.0 28 28679.0 29 37266.0 30 48217.0 31 61710.0 32 79074.0 33 103846.0 34 145046.0 35 224115.0 36 422863.0 37 932791.0 38 1598237.0 39 730487.0 40 2160.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.6405182380979 19.618425800660795 13.769424939905225 23.97163102133608 2 15.626154302240437 20.32073271145515 39.73382305770133 24.319289928603084 3 18.102802483783524 25.459538923867015 30.09255730975413 26.34510128259533 4 12.505794280263247 16.86766134513039 38.36940455475354 32.25713981985282 5 12.957882357424063 37.65363058339785 35.60884562291399 13.779641436264093 6 31.001171929886855 37.877756866008774 17.575116202488182 13.545955001616194 7 27.30538372472658 32.56371019493021 22.52044000239019 17.61046607795303 8 25.421699554587928 35.74745246076475 20.263228941976923 18.5676190426704 9 25.44069086818374 16.336457031611964 20.973529536572038 37.249322563632255 10-11 24.229696743548416 26.40806530375062 28.64801850418714 20.714219448513823 12-13 25.095250281236765 23.840380057173984 28.864472456983115 22.199897204606135 14-15 22.21759506684375 25.49406851483215 26.382424613300852 25.90591180502324 16-17 21.56173374949487 28.961169045436236 26.85676255464123 22.62033465042766 18-19 21.448240034406798 27.623537394390752 29.414587534636567 21.513635036565883 20-21 22.484795297391674 26.87641937710704 29.138459543307384 21.5003257821939 22-23 22.211259963999915 26.667428756507533 29.146320816440703 21.97499046305185 24-25 21.982538243126815 26.77268398654077 29.131730469350885 22.113047300981535 26-27 21.74976401205487 26.830324667451848 29.214863776499932 22.205047543993352 28-29 21.801461677381585 26.87650002819953 29.275993064539684 22.0460452298792 30-31 22.06343312664542 27.16671574061244 28.836904184564222 21.932946948177914 32-33 21.83055474006275 26.95000567236588 29.183911091943422 22.035528495627947 34-35 21.974377360418664 26.737746607189095 29.1970744448226 22.09080158756964 36-37 22.235836622006527 26.998209957210445 28.695026764040836 22.07092665674219 38-39 22.072355573337628 27.204125498713545 28.759057382692443 21.964461545256388 40-41 21.98069316759052 26.944880396407527 28.915400108640988 22.159026327360966 42-43 22.011215263254066 26.975736996209477 28.75079781176579 22.26224992877067 44-45 22.063500620496207 27.075468879495567 28.638119078114972 22.222911421893254 46-47 22.236800058195875 27.344982034621353 28.278540876973747 22.13967703020903 48-49 22.261026689563973 27.297578809080285 28.001374357961794 22.440020143393944 50-51 22.300354371315667 27.32503132791384 27.881009785047645 22.493604515722847 52-53 22.30722832614978 27.32556175907427 27.94180369110807 22.42540622366788 54-55 22.360195666312467 27.582853347258773 27.826212998637768 22.23073798779099 56-57 22.152017673533262 27.360028959376336 27.93163889805416 22.55631446903624 58-59 22.08555140159398 27.057758973812724 28.207961057259716 22.64872856733358 60-61 22.290871561182314 27.37654810122965 27.873291470407136 22.459288867180906 62-63 22.122519205938058 27.326189616366204 27.97445227028867 22.576838907407073 64-65 22.156033795176498 27.074418842300435 28.037865856774058 22.73168150574901 66-67 22.058244385060195 27.162974251566887 27.951032749692946 22.827748613679972 68-69 22.345165673803425 27.311033485278525 27.814456338500204 22.529344502417846 70-71 22.368278474284633 27.149056075793226 27.79837980290687 22.68428564701527 72-73 22.264801105376677 27.26565845644765 27.817556558107835 22.651983880067846 74-75 22.25416718897932 27.347695253460742 27.69254068247266 22.705596875087277 76-77 22.299445060754934 27.26679215152405 27.6278858702665 22.80587691745452 78-79 22.383188233348143 27.38577110834565 27.674758664647133 22.556281993659073 80-81 22.25931631969974 27.255728873035128 27.71755038663019 22.76740442063494 82-83 22.193552465726555 27.263025987674073 27.787048900578103 22.756372646021266 84-85 22.207626344372365 27.225689149153776 27.660491148944207 22.906193357529656 86-87 22.229416136793542 27.31462848188292 27.600877963087534 22.855077418236007 88-89 22.04359945280118 27.286859250023248 27.636506609459843 23.033034687715727 90-91 22.00280614042675 27.20361643756132 27.743529460414983 23.05004796159695 92-93 22.115451231773672 27.28249649048208 27.553093095537694 23.04895918220655 94-95 21.927654213484544 27.093990243940457 27.732338602944917 23.246016939630078 96-97 21.930555922407198 27.12942222814958 27.68423181194064 23.25579003750258 98-99 21.86797347626834 27.23971653316576 27.596090612826803 23.296219377739096 100 21.96389863871879 27.087582195179614 27.53316602019016 23.415353145911435 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 22.0 1 29.0 2 125.5 3 225.5 4 160.5 5 145.5 6 349.0 7 769.0 8 1349.0 9 1614.5 10 1309.5 11 1014.0 12 1137.5 13 1368.5 14 1633.5 15 1944.5 16 2255.5 17 2651.0 18 3086.5 19 3622.5 20 4292.0 21 5265.5 22 6825.0 23 9465.5 24 12968.0 25 17250.0 26 23509.5 27 31584.0 28 41607.0 29 53263.0 30 64613.5 31 76900.5 32 88905.5 33 101159.0 34 114638.0 35 126390.5 36 136257.0 37 146692.0 38 155050.0 39 158863.5 40 163246.0 41 167167.5 42 166920.0 43 167896.0 44 167765.0 45 164273.5 46 162611.0 47 158824.0 48 152891.5 49 148284.0 50 145247.0 51 141472.5 52 132972.5 53 124893.5 54 120215.0 55 114869.5 56 105885.5 57 96152.0 58 89201.0 59 83386.5 60 76290.5 61 66442.0 62 57589.0 63 49357.5 64 41225.0 65 34855.0 66 28519.0 67 22330.5 68 17556.0 69 13768.0 70 10018.0 71 7091.5 72 5367.5 73 4180.0 74 3220.0 75 2285.0 76 1545.5 77 1051.0 78 681.0 79 440.0 80 303.5 81 163.5 82 67.5 83 26.0 84 10.5 85 6.5 86 6.0 87 5.0 88 4.0 89 3.0 90 0.5 91 0.0 92 0.5 93 0.5 94 0.0 95 0.5 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04351700540650079 2 0.2407940965826377 3 0.0026846311793065165 4 0.002273276401832131 5 2.598030173522435E-4 6 1.5155176012214205E-4 7 0.0 8 3.2475377169030443E-4 9 0.0 10-11 8.11884429225761E-4 12-13 0.0 14-15 3.3557889741331456E-4 16-17 9.742613150709133E-5 18-19 6.386824176575986E-4 20-21 1.0825125723010148E-5 22-23 1.2990150867612176E-4 24-25 0.001028386943685964 26-27 8.660100578408119E-5 28-29 2.7062814307525366E-4 30-31 8.660100578408119E-5 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 2.1650251446020297E-5 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 2.1650251446020297E-5 68-69 7.577588006107103E-5 70-71 3.247537716903044E-5 72-73 1.2990150867612176E-4 74-75 6.495075433806088E-5 76-77 0.0 78-79 0.0 80-81 0.0 82-83 4.3300502892040594E-5 84-85 0.0 86-87 2.3815276590622323E-4 88-89 0.0018943970015267756 90-91 0.001829446247188715 92-93 3.4640402313632475E-4 94-95 2.3815276590622323E-4 96-97 4.3300502892040594E-5 98-99 3.247537716903044E-5 100 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 4618884.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.60674194484971 #Duplication Level Percentage of deduplicated Percentage of total 1 68.23337885340239 34.530689956592994 2 16.424055237429286 16.623358501770824 3 6.3200182481376626 9.595065977107314 4 3.0401516620949294 6.154086825473761 5 1.7243894695888786 4.363286644995033 6 1.026235761228843 3.1160669005850683 7 0.6891696123582887 2.4413640110193615 8 0.4839304685081624 1.9592115471234235 9 0.3501539333176309 1.5948134769941513 >10 1.5991032653083765 13.992343395999619 >50 0.08044626337614447 2.762922105233932 >100 0.02823917689022237 2.315277344327164 >500 5.567430305144659E-4 0.18716831809511866 >1k 8.565266432605682E-5 0.09447265670213732 >5k 8.565266432605682E-5 0.2698723379800445 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6928 0.1499929420180286 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5524 0.11959598898781611 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 2.1650251446020294E-5 0.0 10-11 0.0 0.0 0.0 2.1650251446020294E-5 0.0 12-13 0.0 0.0 0.0 2.1650251446020294E-5 0.0 14-15 0.0 0.0 0.0 3.247537716903044E-5 0.0 16-17 0.0 0.0 0.0 4.330050289204059E-5 0.0 18-19 0.0 0.0 0.0 4.330050289204059E-5 0.0 20-21 0.0 0.0 0.0 4.330050289204059E-5 0.0 22-23 0.0 0.0 0.0 7.577588006107103E-5 0.0 24-25 0.0 0.0 0.0 1.8402713729117248E-4 0.0 26-27 0.0 0.0 0.0 2.598030173522435E-4 0.0 28-29 0.0 0.0 0.0 0.0010608623208549944 0.0 30-31 0.0 0.0 0.0 0.0038862201345606427 0.0 32-33 0.0 0.0 0.0 0.010695224214334026 0.0 34-35 0.0 0.0 0.0 0.020123908719075864 0.0 36-37 0.0 0.0 0.0 0.0369136787154646 0.0 38-39 0.0 0.0 0.0 0.06628224480199113 0.0 40-41 0.0 0.0 0.0 0.10064119384682534 0.0 42-43 0.0 0.0 0.0 0.13663473687583405 0.0 44-45 0.0 0.0 0.0 0.1795347101161233 0.0 46-47 0.0 0.0 0.0 0.23421242014304755 0.0 48-49 0.0 0.0 0.0 0.29294955231610065 0.0 50-51 0.0 0.0 0.0 0.35608168553269576 0.0 52-53 0.0 0.0 0.0 0.42079428710485045 0.0 54-55 0.0 0.0 0.0 0.4929545751744361 0.0 56-57 0.0 0.0 0.0 0.5817639066060113 0.0 58-59 0.0 0.0 0.0 0.6821777728126535 0.0 60-61 0.0 0.0 0.0 0.7891841405846087 0.0 62-63 0.0 0.0 0.0 0.9015922460923461 0.0 64-65 0.0 0.0 0.0 1.018925783804053 0.0 66-67 0.0 0.0 0.0 1.1567079840065264 0.0 68-69 0.0 0.0 0.0 1.3101757913816412 0.0 70-71 0.0 0.0 0.0 1.478712173763186 0.0 72-73 0.0 0.0 1.0825125723010147E-5 1.6560602084832614 0.0 74-75 0.0 0.0 2.1650251446020294E-5 1.8388641065677338 0.0 76-77 0.0 0.0 2.1650251446020294E-5 2.036487601767007 0.0 78-79 0.0 0.0 2.1650251446020294E-5 2.2699855636123356 0.0 80-81 0.0 0.0 2.1650251446020294E-5 2.5226006974845006 0.0 82-83 0.0 0.0 2.1650251446020294E-5 2.7863224103484736 0.0 84-85 0.0 0.0 2.1650251446020294E-5 3.0507802317616117 0.0 86-87 0.0 0.0 2.1650251446020294E-5 3.334712454350445 0.0 88 0.0 0.0 2.1650251446020294E-5 3.5691089016307838 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5545 0.0 52.68218 1 GTATCAA 11060 0.0 46.52015 1 TCAACGC 13565 0.0 37.349937 4 ATCAACG 13640 0.0 37.179024 3 CAACGCA 13915 0.0 36.275772 5 TATCAAC 14620 0.0 34.85688 2 AACGCAG 14565 0.0 34.75368 6 ACGCAGA 16840 0.0 29.779535 7 CGCAGAG 17205 0.0 29.01118 8 GTACATG 13490 0.0 26.614397 1 GCAGAGT 19235 0.0 25.63151 9 TACATGG 13685 0.0 25.433628 2 ACATGGG 14300 0.0 23.565357 3 AGAGTAC 17655 0.0 21.616077 10-11 GTGGTAT 2845 0.0 20.535866 1 CAGAGTA 18485 0.0 20.378521 10-11 CATGGGG 10710 0.0 20.31807 4 GAGTACT 12940 0.0 19.957687 12-13 AGTACTT 13330 0.0 19.567692 12-13 GTACTTT 14040 0.0 18.21014 14-15 >>END_MODULE