FastQCFastQC Report
Wed 25 May 2016
SRR1033091_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033091_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2743810
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA31300.033.339921
TCAACGC30200.032.994184
ATCAACG31850.031.4324723
CAACGCA32950.030.2415945
AACGCAG34850.028.723526
GGTATCA23500.028.6038251
GTACATG74650.026.8877221
TATCAAC38750.026.1993452
TACATGG75600.025.1170142
ACATGGG73350.024.413683
ACGCAGA42250.022.913197
CGCAGAG42900.022.4806658
GTATAGG10150.020.3771081
TACACCG3800.019.7906765
CATGGGG55650.019.6788234
GAGTACA50100.019.0464571
TAATACG2753.207242E-818.8004574
ACACCGT4300.018.5798136
GCAGAGT53450.017.781649
GTATTAA17850.017.3804741
Produced by FastQC (version 0.11.5)