FastQCFastQC Report
Wed 25 May 2016
SRR1033091_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033091_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2743810
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44540.16232902424001663No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37500.13667127096992868No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA41400.047.9260521
GTATCAA66300.040.233961
TCAACGC78450.033.4292374
ATCAACG79350.033.1099133
CAACGCA81350.032.2970775
CGCGCGT753.2762246E-431.3341357
TATCAAC84050.031.2188512
AACGCAG84900.031.1126926
ACGCAGA98100.026.6867167
CGCAGAG99200.026.391288
GTACATG75900.024.775421
TAGCGCG1151.3781604E-424.5210274
GCAGAGT111200.023.2474219
TACATGG75800.023.2435192
ACATGGG72350.022.736453
GTGGTAT20950.021.8211481
GAGTACT76600.021.1060512-13
CAGAGTA112700.019.9977710-11
AGAGTAC103050.019.612710-11
TGGTATC20850.018.9789712
Produced by FastQC (version 0.11.5)