FastQCFastQC Report
Wed 25 May 2016
SRR1033090_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033090_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2060164
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA13350.035.2185551
GTATCAA22850.031.0701661
TCAACGC21050.031.042184
ATCAACG21750.030.2592583
TACACCG2350.030.0056025
CAACGCA22400.029.1706265
AACGCAG23950.027.2754616
GTACATG53200.026.6899741
TATCAAC26450.025.9481322
TACATGG54100.024.9381122
CGCGTAT902.1371307E-723.53012312-13
ACGCAGA28050.023.121697
ACATGGG56300.022.7117183
CGCAGAG30200.021.6496458
GAGTACA40300.020.533381
GAACCGT1656.866446E-519.9377846
GTACTAG4300.019.681441
CATGGGG43150.019.6101634
GTCCTAG7350.019.190521
CGACGCG1152.2922013E-618.38088890-91
Produced by FastQC (version 0.11.5)