##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1033090_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2060164 Sequences flagged as poor quality 0 Sequence length 100 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.55482621771859 33.0 31.0 34.0 30.0 34.0 2 31.697780856281344 33.0 31.0 34.0 30.0 34.0 3 31.81446865395182 34.0 31.0 34.0 30.0 34.0 4 35.186008492527776 37.0 35.0 37.0 33.0 37.0 5 35.18086230028289 37.0 35.0 37.0 33.0 37.0 6 35.302660370727764 37.0 35.0 37.0 33.0 37.0 7 35.21967911292499 37.0 35.0 37.0 33.0 37.0 8 35.21980531646995 37.0 35.0 37.0 33.0 37.0 9 36.8572006888772 39.0 38.0 39.0 34.0 39.0 10-11 36.84569650765667 39.0 38.0 39.0 34.0 39.0 12-13 36.76751268345627 39.0 38.0 39.0 33.0 39.0 14-15 38.16121289373079 41.0 39.0 41.0 33.5 41.0 16-17 38.15816362192524 41.0 39.0 41.0 33.0 41.0 18-19 38.160333352102064 41.0 38.5 41.0 33.5 41.0 20-21 38.178435551732775 40.5 39.0 41.0 34.0 41.0 22-23 38.140613805502866 40.0 39.0 41.0 33.5 41.0 24-25 38.14970992600589 40.0 38.0 41.0 33.0 41.0 26-27 38.06126429740545 40.0 38.0 41.0 33.0 41.0 28-29 38.06922749839333 40.0 38.0 41.0 33.0 41.0 30-31 38.219493448094426 40.0 39.0 41.0 33.0 41.0 32-33 38.20816667993422 41.0 38.5 41.0 33.0 41.0 34-35 38.163124149339566 41.0 38.0 41.0 33.0 41.0 36-37 38.08269851332224 40.0 38.0 41.0 33.0 41.0 38-39 37.991232494112126 40.0 38.0 41.0 33.0 41.0 40-41 37.91644670035978 40.0 38.0 41.0 33.0 41.0 42-43 37.79067855762939 40.0 38.0 41.0 32.5 41.0 44-45 37.65062781409635 40.0 38.0 41.0 32.0 41.0 46-47 37.5135011096204 40.0 38.0 41.0 32.0 41.0 48-49 37.390397317883426 40.0 37.5 41.0 31.5 41.0 50-51 37.2589682180642 40.0 37.0 41.0 31.0 41.0 52-53 37.0997641449904 40.0 37.0 41.0 31.0 41.0 54-55 36.93581214893571 40.0 36.5 41.0 31.0 41.0 56-57 36.759033504128794 40.0 36.0 41.0 30.0 41.0 58-59 36.55617028547242 39.5 35.5 41.0 30.0 41.0 60-61 36.32986767072913 39.0 35.0 41.0 30.0 41.0 62-63 36.0753209453228 39.0 35.0 41.0 30.0 41.0 64-65 35.79601041470485 38.5 35.0 40.5 30.0 41.0 66-67 35.496950728194456 38.0 35.0 40.0 29.0 41.0 68-69 35.17681771936603 37.0 34.5 40.0 29.0 41.0 70-71 34.84108425348661 37.0 34.0 39.5 29.0 41.0 72-73 34.491697020237226 36.0 34.0 39.0 28.5 41.0 74-75 34.13817443659825 36.0 34.0 39.0 28.0 40.5 76-77 33.77933407243307 35.0 34.0 38.5 28.0 40.0 78-79 33.39902478637623 35.0 34.0 37.0 27.0 39.0 80-81 33.05283584219509 35.0 33.0 37.0 27.0 39.0 82-83 32.83505536452438 35.0 33.0 36.5 27.0 39.0 84-85 32.61849906123979 35.0 33.0 36.0 27.0 37.0 86-87 32.44796021093467 35.0 33.0 36.0 27.0 37.0 88-89 32.235534161358025 35.0 33.0 35.0 27.0 37.0 90-91 32.03172975549519 35.0 33.0 35.0 26.5 36.0 92-93 31.82237676223835 35.0 33.0 35.0 26.0 36.0 94-95 31.630008581841057 35.0 33.0 35.0 25.5 36.0 96-97 31.46127128714025 35.0 33.0 35.0 25.0 36.0 98-99 31.289164843187244 35.0 33.0 35.0 25.0 35.0 100 31.149526930865697 35.0 33.0 35.0 24.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.20777399547919373 1101 2 0.2883380021752764 1101 3 0.2732278872464313 1101 4 0.16954651301111312 1101 5 0.225987601665949 1101 6 0.2435309949224873 1101 7 0.30933966775431543 1101 8 0.42747296702779636 1101 9 0.3966555363051043 1101 10-11 0.3977374693716058 1101 12-13 0.44133207394217067 1101 14-15 0.5194180795815626 1101 16-17 0.5742650026685752 1101 18-19 0.5449654986718073 1101 20-21 0.5025923996177184 1101 22-23 0.5209481895863277 1101 24-25 0.4447704158842001 1101 26-27 0.4091359699747983 1101 28-29 0.4144532461415551 1101 30-31 0.43898687000109504 1101 32-33 0.3911064418266008 1101 34-35 0.4326410542214134 1101 36-37 0.46109252084080765 1101 38-39 0.4896600572636203 1101 40-41 0.45855126534137725 1101 42-43 0.43355601713610525 1101 44-45 0.4024927073382756 1101 46-47 0.43718092078258763 1101 48-49 0.44471560749016703 1101 50-51 0.4887915541925736 1101 52-53 0.3692495373352713 1101 54-55 0.3406402358689107 1101 56-57 0.4039026967405377 1101 58-59 0.41358557350399394 1101 60-61 0.4969543775980583 1101 62-63 0.415730359948725 1101 64-65 0.38461057160859013 1101 66-67 0.4455689684620978 1101 68-69 0.44087703863441163 1101 70-71 0.2869653791351823 1101 72-73 0.40076226354486266 1101 74-75 0.41954633740853353 1101 76-77 0.3710554248225719 1101 78-79 0.36160414396702123 1101 80-81 0.3826791224317887 1101 82-83 0.31270219467268845 1101 84-85 0.3927686216987496 1101 86-87 0.3087258882245578 1101 88-89 0.3155262704646873 1101 90-91 0.30301119388488473 1101 92-93 0.36732781253938995 1101 94-95 0.35630484373643867 1101 96-97 0.5293347470904983 1101 98-99 0.4277562268579089 1101 100 0.46663342345447134 1102 1 -0.16662985766511795 1102 2 -0.04324324100902288 1102 3 -0.027315551503114932 1102 4 -0.18670575889001384 1102 5 -0.1386552554769125 1102 6 -0.07104188766565755 1102 7 -0.06598657724750723 1102 8 -0.05219079160542606 1102 9 0.007133544302192263 1102 10-11 -0.06450807770217892 1102 12-13 -0.10268782791169428 1102 14-15 -0.02383437407165445 1102 16-17 -0.03525426305297685 1102 18-19 -0.08313928140815818 1102 20-21 3.059583236506569E-4 1102 22-23 -0.003825983441657854 1102 24-25 0.024046133804965564 1102 26-27 -0.06211630192632356 1102 28-29 -0.05224477276066608 1102 30-31 0.01516916734751561 1102 32-33 0.01937479878770887 1102 34-35 0.015931675813561696 1102 36-37 0.058723836725945944 1102 38-39 0.07151710924472354 1102 40-41 -0.014918382059562418 1102 42-43 -0.029123463052918908 1102 44-45 -0.03742686736183032 1102 46-47 -0.08089705086408117 1102 48-49 -0.09814744230990868 1102 50-51 -0.0537915973886669 1102 52-53 -0.05093857607803187 1102 54-55 -0.07667392261890171 1102 56-57 -0.1398164054041331 1102 58-59 -0.08509717822265372 1102 60-61 -0.00979634941030838 1102 62-63 -0.024287815260287005 1102 64-65 -0.16526810887123844 1102 66-67 -0.16507943211951215 1102 68-69 -0.13529253606569824 1102 70-71 -0.15999799781864965 1102 72-73 -0.05677317811271365 1102 74-75 -0.20809951864380594 1102 76-77 -0.2254735646358057 1102 78-79 -0.2374352962365407 1102 80-81 -0.15382714801533126 1102 82-83 -0.23395765629059184 1102 84-85 -0.30887487157642113 1102 86-87 -0.21030446437450223 1102 88-89 -0.16219001452259363 1102 90-91 -0.17224007837974398 1102 92-93 -0.228650468085835 1102 94-95 -0.17401594870267445 1102 96-97 -0.23357510387279135 1102 98-99 -0.19465244271678728 1102 100 -0.26561394114022363 1103 1 -0.05492408194630016 1103 2 0.006790496842832283 1103 3 -0.04072735471576294 1103 4 0.022390872360297465 1103 5 -0.11763493723329077 1103 6 0.003559552487416795 1103 7 0.023391160805772415 1103 8 0.08167773148234403 1103 9 0.06250768197276813 1103 10-11 0.05359895185620189 1103 12-13 0.12281515562222012 1103 14-15 0.2049605048245624 1103 16-17 0.2018340847993727 1103 18-19 0.16286360141574363 1103 20-21 0.1531892857822328 1103 22-23 0.08516433580906124 1103 24-25 0.07855353599036619 1103 26-27 0.06995152626240042 1103 28-29 0.04695968113932736 1103 30-31 0.10430016588721713 1103 32-33 0.06205462909134951 1103 34-35 0.014272856134823542 1103 36-37 0.15733762644345006 1103 38-39 0.14835512978956444 1103 40-41 0.2310726043949458 1103 42-43 0.10386944948043464 1103 44-45 0.176901333957467 1103 46-47 0.22605911510600407 1103 48-49 0.21943507695088726 1103 50-51 0.2122309238863238 1103 52-53 0.2784681161018412 1103 54-55 0.2481866569671709 1103 56-57 0.32247103354042395 1103 58-59 0.32121790561842545 1103 60-61 0.16577977476512729 1103 62-63 0.16904568592305225 1103 64-65 0.2625767599539799 1103 66-67 0.20996053716393703 1103 68-69 0.10490749918280784 1103 70-71 0.16587396199805937 1103 72-73 0.18093365506116044 1103 74-75 0.134654837063934 1103 76-77 0.1308679555817278 1103 78-79 0.10358728798220085 1103 80-81 0.11558388728352753 1103 82-83 0.11771545162017816 1103 84-85 0.15160230501857086 1103 86-87 0.09438975487118739 1103 88-89 0.10308152482984667 1103 90-91 0.1118060090365347 1103 92-93 0.18836203254359773 1103 94-95 0.1642167165876245 1103 96-97 0.18834367350774173 1103 98-99 0.21666440354938743 1103 100 0.2150730269850598 1104 1 -0.03070505844389615 1104 2 0.13787625278508742 1104 3 0.10091637840683987 1104 4 0.10702750954512652 1104 5 0.02705842181683238 1104 6 0.1398147684550537 1104 7 0.13146474910926997 1104 8 0.0976132969806045 1104 9 0.21427031723964518 1104 10-11 0.20475755870570822 1104 12-13 0.238727662475263 1104 14-15 0.2777383930249613 1104 16-17 0.16390915870414347 1104 18-19 0.2448258560948844 1104 20-21 0.27855127348549047 1104 22-23 0.23966908835196676 1104 24-25 0.13369264147105753 1104 26-27 -0.015660959993979873 1104 28-29 -0.017774402840977643 1104 30-31 0.129145195578225 1104 32-33 0.10142426337763766 1104 34-35 0.12992108929616109 1104 36-37 0.20435750671864383 1104 38-39 0.20332868140339144 1104 40-41 0.17974820348436538 1104 42-43 0.2196460064043677 1104 44-45 0.20026022195430215 1104 46-47 0.24427148162557444 1104 48-49 0.17566826710844907 1104 50-51 0.1684957596325063 1104 52-53 0.16206032876500132 1104 54-55 0.11874923386685765 1104 56-57 0.08674814801518949 1104 58-59 0.16974554506768413 1104 60-61 0.21591641525262872 1104 62-63 0.2815067819653123 1104 64-65 0.2697790437206393 1104 66-67 0.2818373967926249 1104 68-69 0.2621052492835432 1104 70-71 0.3045015762869667 1104 72-73 0.2628228306485383 1104 74-75 0.26927240883246384 1104 76-77 0.18435212287940317 1104 78-79 0.1586689870440594 1104 80-81 0.12179861251035362 1104 82-83 0.1084526016797156 1104 84-85 0.07696201087672705 1104 86-87 0.13031308247389006 1104 88-89 0.13497535418025564 1104 90-91 0.16915898465261847 1104 92-93 0.22347626591270497 1104 94-95 0.20289753389650045 1104 96-97 0.2226271351147986 1104 98-99 0.2194577422430548 1104 100 0.1428643990885412 1105 1 -0.16064756484876241 1105 2 -0.08652650796041428 1105 3 -0.04133949698994144 1105 4 -0.14028078648250641 1105 5 -0.2274410657365209 1105 6 -0.06558529185971906 1105 7 -0.09513852791692301 1105 8 -0.027506545720306974 1105 9 0.024129321450807595 1105 10-11 0.0021092023976336804 1105 12-13 0.033129089886664076 1105 14-15 0.011718555531494701 1105 16-17 -0.0017071878574341781 1105 18-19 -0.05511268464248076 1105 20-21 -0.033509963745551374 1105 22-23 0.03085340376502188 1105 24-25 -0.11942464214398285 1105 26-27 -0.08318569105247775 1105 28-29 -0.009326386561710365 1105 30-31 -0.034876300285283435 1105 32-33 -0.08540931993199763 1105 34-35 -0.11213552990648168 1105 36-37 -0.04547760048313165 1105 38-39 0.012670775447517713 1105 40-41 0.04469748242529903 1105 42-43 -0.01016951852583503 1105 44-45 -0.009571943901015345 1105 46-47 -0.0995787294475079 1105 48-49 -0.023741460736893316 1105 50-51 -0.065571613224364 1105 52-53 -0.046142880507225925 1105 54-55 -0.07843594334926962 1105 56-57 -0.025024848707552394 1105 58-59 -0.17893631397772936 1105 60-61 -0.1568119811584694 1105 62-63 -0.2007785297859428 1105 64-65 -0.12821036857659607 1105 66-67 -0.22004638110938401 1105 68-69 -0.20939482024439826 1105 70-71 -0.17184759472829114 1105 72-73 -0.13887788563226877 1105 74-75 -0.16605413498815835 1105 76-77 -0.17278095702507557 1105 78-79 -0.1504245231668193 1105 80-81 -0.0671311093378435 1105 82-83 -0.13452144294062407 1105 84-85 -0.06295658135136506 1105 86-87 -0.1673862581265979 1105 88-89 -0.22207400607688754 1105 90-91 -0.16859085096960058 1105 92-93 -0.15092474948129464 1105 94-95 -0.18398703656677284 1105 96-97 -0.07365452227098501 1105 98-99 -0.12675493084001843 1105 100 -0.06878527853645267 1106 1 -0.14703858912363543 1106 2 -0.11606248203658609 1106 3 -0.07725729498727318 1106 4 -0.08333904699070871 1106 5 -0.06370403653535561 1106 6 -0.015350062294828604 1106 7 0.035224058660972446 1106 8 0.09018228405785322 1106 9 0.0474252948052154 1106 10-11 0.10969469708608415 1106 12-13 0.05140293761167669 1106 14-15 0.006030278962832369 1106 16-17 0.09716993328785861 1106 18-19 0.06508687787310663 1106 20-21 0.10835378013825903 1106 22-23 0.1127211481853081 1106 24-25 0.03444164641550884 1106 26-27 0.056138108255694874 1106 28-29 0.044059155459819976 1106 30-31 0.06731331128088414 1106 32-33 0.027133385189841874 1106 34-35 0.17442765166869378 1106 36-37 0.05543626244303823 1106 38-39 0.12231193634686832 1106 40-41 0.10048637204646838 1106 42-43 0.11939351088372518 1106 44-45 0.0948127915217043 1106 46-47 0.027562192971018362 1106 48-49 0.07129906229914695 1106 50-51 0.09126812138911333 1106 52-53 0.0727658598398051 1106 54-55 0.0897972883679472 1106 56-57 0.11025126807862051 1106 58-59 0.09327076197668305 1106 60-61 0.06745769813075242 1106 62-63 0.0813127649652543 1106 64-65 0.17000123977880577 1106 66-67 0.11975837378568599 1106 68-69 0.11990808627299288 1106 70-71 0.09361770804443381 1106 72-73 0.0670057746737669 1106 74-75 0.1273189807244961 1106 76-77 0.11995136868505085 1106 78-79 0.08850207215988348 1106 80-81 0.12421336248956294 1106 82-83 0.10625039433876537 1106 84-85 0.15465730988443482 1106 86-87 0.079101857276342 1106 88-89 0.1380602077412192 1106 90-91 0.008974710352646298 1106 92-93 -0.011077062536259064 1106 94-95 -0.012715003496552413 1106 96-97 -0.03213085699617935 1106 98-99 -0.014283192330751149 1106 100 -0.03714253128574896 1107 1 0.09748245541156209 1107 2 0.10780352959231365 1107 3 0.11377099001772706 1107 4 0.14694043901008058 1107 5 0.15471811391539347 1107 6 0.10594506747274579 1107 7 0.17465691623006308 1107 8 0.13047386000131667 1107 9 0.22029771865790337 1107 10-11 0.20737785930068497 1107 12-13 0.19012437380640534 1107 14-15 0.24918105704511362 1107 16-17 0.17091420656203837 1107 18-19 0.17746722511427038 1107 20-21 0.14792606650055262 1107 22-23 0.18355032899868462 1107 24-25 0.1674163215236959 1107 26-27 0.06708987052038395 1107 28-29 0.09070662717570599 1107 30-31 0.14754030956832054 1107 32-33 0.1617749737797567 1107 34-35 0.09831395389183228 1107 36-37 0.1627920040819788 1107 38-39 0.15004757376905786 1107 40-41 0.132041528853577 1107 42-43 0.12747254394675878 1107 44-45 0.10812475529168353 1107 46-47 0.13000733984542734 1107 48-49 0.11282495667195747 1107 50-51 0.1164203816122864 1107 52-53 0.13151051714500994 1107 54-55 0.09458959949372314 1107 56-57 0.14863451728119514 1107 58-59 0.1491905246090397 1107 60-61 0.15374206929793388 1107 62-63 0.10427406829045793 1107 64-65 0.19059770404636112 1107 66-67 0.15714256915315872 1107 68-69 0.19423418999220843 1107 70-71 0.24274659333629245 1107 72-73 0.20435949767475137 1107 74-75 0.17876772602689073 1107 76-77 0.20696081334700978 1107 78-79 0.2675909525703233 1107 80-81 0.2990836855831489 1107 82-83 0.17010573855509392 1107 84-85 0.2275397800303196 1107 86-87 0.19918726327424707 1107 88-89 0.20723967049173098 1107 90-91 0.25094994707364293 1107 92-93 0.1856817781756952 1107 94-95 0.1699587569925214 1107 96-97 0.23322254168877876 1107 98-99 0.09671766546011185 1107 100 0.13913072955656247 1108 1 -0.1092113854490755 1108 2 -0.02745554692173613 1108 3 0.04873841477352414 1108 4 0.11762088612488952 1108 5 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2214 44-45 -0.16796238509121508 2214 46-47 -0.14526590847867737 2214 48-49 -0.11917140252690217 2214 50-51 -0.09925773073248934 2214 52-53 -0.142027694719431 2214 54-55 -0.04995946000391882 2214 56-57 -0.13410909743966926 2214 58-59 -0.05301240347621672 2214 60-61 -0.022746168578478887 2214 62-63 -0.0465529304422958 2214 64-65 -0.13399235999160197 2214 66-67 -0.09547539485242851 2214 68-69 -0.041678061657329124 2214 70-71 -0.016176451313427265 2214 72-73 -0.002844410127814001 2214 74-75 -0.04466420533301374 2214 76-77 0.030868227171723106 2214 78-79 0.012707600217574111 2214 80-81 7.321757945533136E-5 2214 82-83 -0.03824248607281078 2214 84-85 -0.10293169601230545 2214 86-87 -0.1637345689424663 2214 88-89 -0.12227905454146537 2214 90-91 -0.10797824568849279 2214 92-93 -0.1076404633684831 2214 94-95 -0.12710090034981647 2214 96-97 -0.045153451227140096 2214 98-99 -0.04518186719984385 2214 100 -0.08287846665323428 2215 1 -0.053393872371557904 2215 2 -0.16108654919990428 2215 3 -0.1984395707579374 2215 4 -0.13384406764944146 2215 5 -0.1879910967383296 2215 6 -0.2254644324861914 2215 7 -0.19613695817461974 2215 8 -0.16281827900261447 2215 9 -0.29074861466380497 2215 10-11 -0.22578475207583182 2215 12-13 -0.24684282355283216 2215 14-15 -0.3137905011231652 2215 16-17 -0.3822963924544922 2215 18-19 -0.3721042872831575 2215 20-21 -0.29658711676258065 2215 22-23 -0.23164034306717696 2215 24-25 -0.2530598624805265 2215 26-27 -0.2721873704173987 2215 28-29 -0.2411449641717951 2215 30-31 -0.22347152931320835 2215 32-33 -0.25993711608171566 2215 34-35 -0.2842567978247672 2215 36-37 -0.2600141290095692 2215 38-39 -0.2711553976726151 2215 40-41 -0.2632224035885784 2215 42-43 -0.2299182456780997 2215 44-45 -0.23064906536812657 2215 46-47 -0.21967306193182168 2215 48-49 -0.17608640868976977 2215 50-51 -0.21585329431472644 2215 52-53 -0.2642222246396031 2215 54-55 -0.21818431757019852 2215 56-57 -0.280382252730071 2215 58-59 -0.3003905015748103 2215 60-61 -0.35765856181981803 2215 62-63 -0.3307097536296979 2215 64-65 -0.4032571074688107 2215 66-67 -0.41378148457753383 2215 68-69 -0.32365616036921807 2215 70-71 -0.28723234379901896 2215 72-73 -0.4498505385342 2215 74-75 -0.36331030778833195 2215 76-77 -0.27127232709648297 2215 78-79 -0.26062835616149727 2215 80-81 -0.42691403161460784 2215 82-83 -0.3380428152945001 2215 84-85 -0.35720897876666413 2215 86-87 -0.2945582452182265 2215 88-89 -0.3746557121308989 2215 90-91 -0.42733857930837615 2215 92-93 -0.339358262877937 2215 94-95 -0.48769852839414085 2215 96-97 -0.3685769990325518 2215 98-99 -0.3515668857293335 2215 100 -0.331693897930581 2216 1 -0.19900312955451938 2216 2 -0.3825796465389786 2216 3 -0.25339389251693945 2216 4 -0.3054246528992337 2216 5 -0.2237451172280629 2216 6 -0.36389903052280204 2216 7 -0.3359151486760723 2216 8 -0.17286821096026728 2216 9 -0.2790553957899746 2216 10-11 -0.24556568138881119 2216 12-13 -0.31316133021584136 2216 14-15 -0.4845325307536896 2216 16-17 -0.5125054151267108 2216 18-19 -0.4442556408551681 2216 20-21 -0.39537146103118914 2216 22-23 -0.3472718359762226 2216 24-25 -0.3989054790982678 2216 26-27 -0.4464987904055846 2216 28-29 -0.4432873994887814 2216 30-31 -0.5533400492423439 2216 32-33 -0.5809189800141326 2216 34-35 -0.8063701675044115 2216 36-37 -0.8751114167302774 2216 38-39 -0.7475854253174887 2216 40-41 -0.6847202253775393 2216 42-43 -0.7656084860944006 2216 44-45 -0.6367482959332591 2216 46-47 -0.694244977131369 2216 48-49 -0.6729043547523048 2216 50-51 -0.6372766204301428 2216 52-53 -0.6340053963545245 2216 54-55 -0.7182280700843435 2216 56-57 -0.8235641012566717 2216 58-59 -0.7845176851350999 2216 60-61 -0.6917375936499326 2216 62-63 -0.7668776770114434 2216 64-65 -0.7795273254610962 2216 66-67 -0.6849676809674392 2216 68-69 -0.6625667296140776 2216 70-71 -0.6534092881412334 2216 72-73 -0.6730496758085707 2216 74-75 -0.7664209472152379 2216 76-77 -0.6325392328385675 2216 78-79 -0.6149721625959899 2216 80-81 -0.5604388991674014 2216 82-83 -0.6516500526039621 2216 84-85 -0.5250926135119016 2216 86-87 -0.5486109159873997 2216 88-89 -0.6770356366424082 2216 90-91 -0.5772642719281329 2216 92-93 -0.7586884865190164 2216 94-95 -0.710552815062119 2216 96-97 -0.7519022928128827 2216 98-99 -0.6463955302743933 2216 100 -0.6014319134904547 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 22073.0 3 4645.0 4 2627.0 5 2248.0 6 1872.0 7 1950.0 8 2356.0 9 2635.0 10 2947.0 11 3353.0 12 3602.0 13 4087.0 14 4461.0 15 4768.0 16 4571.0 17 4758.0 18 4619.0 19 4519.0 20 4607.0 21 4696.0 22 5542.0 23 7669.0 24 11942.0 25 15794.0 26 16132.0 27 14595.0 28 15526.0 29 19218.0 30 23323.0 31 28579.0 32 37570.0 33 50845.0 34 72562.0 35 112395.0 36 205469.0 37 421122.0 38 647237.0 39 262746.0 40 504.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.77651655896117 19.1768240114044 12.985081513754004 24.061577915880427 2 15.688044614171332 19.458192667643072 40.50715404009992 24.346608678085676 3 18.962264884039897 24.14666651125021 29.263656249878583 27.627412354831314 4 12.413768982239604 15.936604505362705 38.37855325714125 33.27107325525645 5 13.060244077935087 37.68958211163998 35.53481868572597 13.715355124698958 6 32.1080080549368 37.330162531912464 17.299798626579836 13.262030786570895 7 28.197516804999818 32.02778387769419 21.67213391918442 18.10256539812157 8 25.132260735373574 37.01313278161527 19.786147704435642 18.06845877857551 9 24.694994854632434 17.41340088875414 20.81872078096865 37.07288347564477 10-11 24.115996308183583 27.01680291516475 28.389985644285858 20.477215132365806 12-13 24.661648910801652 24.16025396041757 28.48463659495563 22.693460533825146 14-15 21.760850250616063 26.41785572940317 25.441824542274315 26.379469477706458 16-17 21.66081729309612 29.785997935515212 26.00531908434028 22.547865687048393 18-19 21.522675074253904 28.717420235056785 28.05711562035837 21.702789070330944 20-21 22.65621944509561 28.10318797346817 27.562227245288245 21.67836533614798 22-23 22.28674697156756 27.810072530847705 27.65101365429391 22.252166843290823 24-25 22.15639724141949 27.8813742411112 27.673218416753446 22.28901010071586 26-27 21.965462541869773 27.773690220076485 27.74942422981011 22.51142300824363 28-29 22.023776853510938 27.859140891651435 27.846366515786553 22.270715739051074 30-31 22.233942686847026 28.313406789314595 27.432396492280564 22.02025403155782 32-33 21.84362244662522 28.015414604480405 27.955643788279673 22.1853191606147 34-35 21.885539213246858 27.717525477389266 28.060315038195753 22.336620271168123 36-37 22.323602745700253 28.152606462905172 27.40243915468798 22.121351636706596 38-39 22.222052202400338 28.304608484426307 27.568762695319375 21.904576617853976 40-41 21.89579949992543 27.924117303289346 27.985448578158877 22.194634618626345 42-43 21.858417353693817 27.867200873591536 27.863071910029397 22.411309862685243 44-45 22.04044527054876 28.078430686779765 27.78802312282489 22.093100919846588 46-47 22.02687459558402 28.222430246137524 27.674051893492617 22.076643264785837 48-49 21.914179892046604 28.06800437293106 27.78941339718985 22.228402337832485 50-51 21.99095831469281 27.95009634781781 27.804688133747383 22.254257203741997 52-53 22.0436560947056 27.956198174478647 27.74875035825492 22.251395372560832 54-55 22.118806393960714 28.30652911314505 27.59601347687096 21.978651016023274 56-57 21.869436792736483 28.072687636583186 27.72876151136115 22.32911405931918 58-59 21.798010624219653 27.665593299742348 28.124212976916258 22.41218309912174 60-61 22.095795455290574 28.141654428880514 27.60905269942451 22.153497416404406 62-63 21.921392713415894 28.096579662556497 27.706409094993724 22.27561852903389 64-65 21.919907130254433 27.86051080788664 27.879453904524365 22.34012815733456 66-67 21.80279535160472 27.88838022610533 27.705917353766196 22.60290706852376 68-69 22.249754769279118 28.014030670172634 27.663288202137633 22.07292635841062 70-71 22.206585710278773 27.823927748791544 27.68781319400513 22.281673346924553 72-73 21.92499928362135 27.964047019694828 27.924221223555307 22.186732473128515 74-75 21.95262803335961 28.014708846532876 27.763190679281184 22.269472440826327 76-77 21.993099609813243 27.999858184548525 27.58099264987999 22.426049555758244 78-79 22.090763836276395 28.128970219709775 27.671970006022107 22.108295937991723 80-81 22.05322021387154 27.882093843180293 27.790382579473384 22.274303363474782 82-83 21.884966569875445 27.98716022905475 27.94488388210023 22.18298931896957 84-85 21.936448668930097 27.863953367816517 27.74745546286065 22.45214250039274 86-87 22.032092520939194 27.94027667707799 27.7769198794151 22.250710922567713 88-89 21.845344293915574 27.85622296963275 27.812056575718046 22.486376160733624 90-91 21.830097319566203 27.762431777607443 27.888442442625696 22.51902846020066 92-93 22.025800217065633 28.01130003860563 27.652316216550894 22.310583527777844 94-95 21.806875744636013 27.696514101921927 27.87526316441199 22.621346989030073 96-97 21.847052904749695 27.72177027730511 27.91329650435499 22.517880313590204 98-99 21.80740780309312 27.775655913143027 27.917552406886497 22.499383876877353 100 21.98412513403888 27.774896265560166 27.751876544361043 22.489102056039908 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 427.0 1 361.5 2 290.5 3 281.0 4 278.5 5 271.5 6 270.0 7 245.5 8 216.0 9 200.0 10 191.5 11 193.5 12 187.5 13 197.0 14 221.0 15 259.5 16 330.0 17 453.5 18 631.5 19 898.0 20 1309.0 21 2018.5 22 3038.5 23 4470.0 24 6506.5 25 9029.5 26 12238.0 27 16317.5 28 21459.0 29 27200.5 30 32646.5 31 38144.0 32 43585.0 33 49353.0 34 54667.0 35 58911.0 36 62518.0 37 66246.0 38 69649.5 39 71142.0 40 71999.5 41 72737.0 42 73126.5 43 72614.5 44 71252.0 45 70274.5 46 69960.0 47 68208.5 48 66184.5 49 65153.5 50 63933.0 51 61946.5 52 58493.5 53 55899.0 54 53523.5 55 50943.5 56 48136.5 57 44493.0 58 41057.5 59 37722.0 60 33580.5 61 28759.5 62 24730.0 63 20854.5 64 16986.5 65 13801.0 66 11040.0 67 8611.0 68 6808.5 69 5317.5 70 3947.0 71 2984.0 72 2332.0 73 1764.5 74 1320.0 75 950.5 76 635.0 77 467.0 78 324.5 79 206.0 80 137.0 81 82.5 82 54.5 83 42.5 84 26.5 85 17.5 86 14.0 87 10.5 88 8.5 89 7.5 90 9.0 91 8.5 92 4.5 93 2.5 94 1.5 95 1.5 96 2.0 97 1.5 98 1.0 99 1.0 100 6.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0635386309051124 2 0.04407416108620479 3 0.0572769934820723 4 0.07742102085076721 5 0.09159464974633087 6 0.08732314514766785 7 0.0897015965719234 8 0.10232195106797323 9 0.09717672961958368 10-11 0.10186082273061757 12-13 0.10705458400399191 14-15 0.10365679625505542 16-17 0.07470279065161803 18-19 0.09819606594426464 20-21 0.09132768070891444 22-23 0.057179913832102684 24-25 0.01985278841878608 26-27 0.18386885704244904 28-29 0.06577146285441354 30-31 0.07579493671377618 32-33 0.07120792325271193 34-35 0.021624492030731534 36-37 0.052908409233439674 38-39 0.07671719338848752 40-41 0.08111004754961255 42-43 0.0745571711766636 44-45 0.07307670651462699 46-47 0.07960531297508354 48-49 0.0772025916383356 50-51 0.07169332150255998 52-53 0.07640168452608628 54-55 0.0668636089165717 56-57 0.06975172850316771 58-59 0.0668636089165717 60-61 0.06351436099262 62-63 0.07033420640298539 64-65 0.06632967084173881 66-67 0.06742181690389698 68-69 0.06739754699140457 70-71 0.0600194935937139 72-73 0.058102170506814016 74-75 0.06149995825575051 76-77 0.05582079873252809 78-79 0.05261717028353083 80-81 0.04776318778505012 82-83 0.05305402870839408 84-85 0.04366157257383393 86-87 0.04213256808681251 88-89 0.043977081436235174 90-91 0.039535687450125326 92-93 0.042666506161645384 94-95 0.041380200799547996 96-97 0.044462479686083245 98-99 0.044486749598575646 100 0.051452214483895455 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2060164.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 72.2358762728875 #Duplication Level Percentage of deduplicated Percentage of total 1 81.3304775562045 58.74978313964843 2 12.105877384092784 17.489573217841468 3 3.3405631518857732 7.239255195641634 4 1.3054769070318175 3.7720907333384894 5 0.6216718783999469 2.2453506445216065 6 0.3502168411059687 1.517893224168735 7 0.22063635263450482 1.1156502189145086 8 0.14225648766556015 0.822081763361996 9 0.11303394942298013 0.7348595746638794 >10 0.455124029766939 5.4657271866237656 >50 0.012377407964945724 0.5864725796255554 >100 0.0022208682657940703 0.2295710754264375 >500 6.718555850094943E-5 0.03169144622353226 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 4.8539824984807035E-5 0.0 5 0.0 0.0 0.0 4.8539824984807035E-5 0.0 6 0.0 0.0 0.0 4.8539824984807035E-5 0.0 7 0.0 0.0 0.0 4.8539824984807035E-5 0.0 8 0.0 0.0 0.0 4.8539824984807035E-5 0.0 9 0.0 0.0 0.0 4.8539824984807035E-5 0.0 10-11 0.0 0.0 0.0 9.707964996961407E-5 0.0 12-13 0.0 0.0 0.0 9.707964996961407E-5 0.0 14-15 0.0 0.0 0.0 9.707964996961407E-5 0.0 16-17 0.0 0.0 0.0 1.2134956246201759E-4 0.0 18-19 0.0 0.0 0.0 1.456194749544211E-4 0.0 20-21 0.0 0.0 0.0 1.6988938744682462E-4 0.0 22-23 0.0 0.0 0.0 1.9415929993922814E-4 0.0 24-25 0.0 0.0 0.0 3.1550886240124573E-4 0.0 26-27 0.0 0.0 0.0 6.310177248024915E-4 0.0 28-29 0.0 0.0 0.0 0.0010921460621581584 0.0 30-31 0.0 0.0 0.0 0.003397787748936492 0.0 32-33 0.0 0.0 0.0 0.009271106572098144 0.0 34-35 0.0 0.0 0.0 0.01774130603194697 0.0 36-37 0.0 0.0 0.0 0.032521682739820715 0.0 38-39 0.0 0.0 0.0 0.059922413943744285 0.0 40-41 0.0 0.0 0.0 0.09004137534681705 0.0 42-43 0.0 0.0 0.0 0.12435903161107563 0.0 44-45 0.0 0.0 0.0 0.16236571457417953 0.0 46-47 0.0 0.0 0.0 0.20869697752217786 0.0 48-49 0.0 0.0 0.0 0.2596637937562252 0.0 50-51 0.0 0.0 0.0 0.3160185305635862 0.0 52-53 0.0 0.0 0.0 0.3736595727330445 0.0 54-55 0.0 0.0 0.0 0.4395766550624125 0.0 56-57 0.0 0.0 0.0 0.5187693795251251 0.0 58-59 0.0 0.0 0.0 0.6070633211724892 0.0 60-61 0.0 0.0 0.0 0.7049924180793374 0.0 62-63 0.0 0.0 0.0 0.8067561611599854 0.0 64-65 0.0 0.0 0.0 0.9166503249255884 0.0 66-67 0.0 0.0 0.0 1.0407181175867553 0.0 68-69 0.0 0.0 0.0 1.1814593401302034 0.0 70-71 0.0 0.0 0.0 1.335597554369458 0.0 72-73 0.0 0.0 0.0 1.4986185565809325 0.0 74-75 0.0 0.0 0.0 1.6646004881164802 0.0 76-77 0.0 0.0 0.0 1.8449259379350382 0.0 78-79 0.0 2.4269912492403518E-5 0.0 2.0519240215827477 0.0 80-81 0.0 4.8539824984807035E-5 0.0 2.2857888983595482 0.0 82-83 0.0 4.8539824984807035E-5 0.0 2.5289734215334314 0.0 84-85 0.0 4.8539824984807035E-5 0.0 2.7686145374834235 0.0 86-87 0.0 4.8539824984807035E-5 0.0 3.022647711541411 0.0 88 0.0 4.8539824984807035E-5 0.0 3.234839556462495 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1335 0.0 35.218555 1 GTATCAA 2285 0.0 31.070166 1 TCAACGC 2105 0.0 31.04218 4 ATCAACG 2175 0.0 30.259258 3 TACACCG 235 0.0 30.005602 5 CAACGCA 2240 0.0 29.170626 5 AACGCAG 2395 0.0 27.275461 6 GTACATG 5320 0.0 26.689974 1 TATCAAC 2645 0.0 25.948132 2 TACATGG 5410 0.0 24.938112 2 CGCGTAT 90 2.1371307E-7 23.530123 12-13 ACGCAGA 2805 0.0 23.12169 7 ACATGGG 5630 0.0 22.711718 3 CGCAGAG 3020 0.0 21.649645 8 GAGTACA 4030 0.0 20.53338 1 GAACCGT 165 6.866446E-5 19.937784 6 GTACTAG 430 0.0 19.68144 1 CATGGGG 4315 0.0 19.610163 4 GTCCTAG 735 0.0 19.19052 1 CGACGCG 115 2.2922013E-6 18.380888 90-91 >>END_MODULE