FastQCFastQC Report
Wed 25 May 2016
SRR1033090_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033090_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2060164
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39790.1931399636145472No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33130.1608124401746657No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA27900.051.0221821
GTATCAA53700.042.923081
ATCAACG60900.037.278773
TCAACGC61050.037.261454
CAACGCA62750.036.249345
TATCAAC64850.035.160832
AACGCAG65400.034.924246
ACGCAGA77400.029.2060347
CGCAGAG78750.028.705368
GTACATG55650.028.0361981
TACATGG55900.026.1260452
GCAGAGT86050.026.0516959
GTGGTAT15000.025.4536481
ACATGGG56700.024.455173
ATAACGC1351.4572897E-524.3722723
TGGTATC15300.022.1699562
CAGAGTA84900.020.89670210-11
GAGTACT62450.020.8082912-13
AGAGTAC80750.020.57384110-11
GAGTACA39400.020.3380361
Produced by FastQC (version 0.11.5)