FastQCFastQC Report
Wed 25 May 2016
SRR1033089_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033089_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4460350
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA57000.036.046291
GGTATCA35000.034.3895071
TCAACGC56150.033.910944
ATCAACG58300.032.902293
CAACGCA58000.032.667545
AACGCAG60850.031.2839586
GTACATG128650.029.1274551
TATCAAC69500.028.6147582
TACATGG130950.027.3220732
ACATGGG136050.025.4339493
ACGCAGA74850.025.119737
CGCAGAG76850.024.0583638
TACACCG6750.022.2887425
CATGGGG100600.021.4510844
CCGATCG1805.61259E-620.9137769
GCAGAGT92850.019.3596049
GAGTACA91200.018.4046421
GTATATA30200.017.5924171
TACGACC3602.1918822E-916.9775644
AGTACAT92350.016.2909952
Produced by FastQC (version 0.11.5)