FastQCFastQC Report
Wed 25 May 2016
SRR1033089_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033089_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4460350
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67330.15095227952963333No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52790.11835394083423946No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA57200.053.0637661
GTATCAA107700.042.2298771
TCAACGC130200.034.400634
ATCAACG130450.034.263033
CAACGCA134050.033.342135
TATCAAC136200.033.031592
AACGCAG139250.032.096686
GTACATG132300.028.0720791
ACGCAGA162400.027.1740467
CGCAGAG164300.026.8031928
TACATGG134950.025.9020562
ACATGGG142700.024.2405913
TATACCG2950.023.8973035
GCAGAGT185500.023.1825759
CATGGGG103200.021.267764
AGAGTAC168000.020.58984210-11
TACACCG7400.020.3234735
GAGTACT118950.019.93339312-13
GTGGTAT28750.019.672141
CAGAGTA182200.019.29468510-11
Produced by FastQC (version 0.11.5)