FastQCFastQC Report
Wed 25 May 2016
SRR1033088_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033088_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3156510
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA23850.035.288141
GTATCAA36650.033.7400631
TCAACGC36050.031.2963964
ATCAACG36350.031.1674293
CAACGCA37200.030.3303435
AACGCAG39150.029.0501256
GTACATG81100.028.0020871
TACATGG82050.026.4698662
TATCAAC46100.025.2894342
ACATGGG86700.024.3995463
ACGCAGA47900.023.8423427
CGCAGAG49150.022.686358
CTAGCGT1354.0734932E-420.8932424
CATGGGG60400.020.858654
TAGACCG1801.3358131E-418.2824575
TACGTCG1600.001277071417.629515
GCAGAGT62600.017.515599
TCTAGCG1650.001569929517.094473
GTATAGA13350.016.9053481
TAGCGTG2556.0812035E-616.5924825
Produced by FastQC (version 0.11.5)