FastQCFastQC Report
Wed 25 May 2016
SRR1033088_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033088_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3156510
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51110.16191933496171404No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43680.1383806799281485No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA42200.053.2987821
GTATCAA76150.044.5215571
ATCAACG91350.036.4772953
TCAACGC92350.036.0308384
CAACGCA94400.035.1493765
TATCAAC97850.034.1975442
AACGCAG97950.033.8754586
ACGCAGA110750.029.9178437
CGCAGAG113150.029.200198
GTACATG79550.027.3850231
TACATGG79200.026.5403772
GCAGAGT127850.025.3277449
ACATGGG84800.023.665573
GTGGTAT21350.022.0858151
AGAGTAC117500.021.63884210-11
GAGTACT89750.021.31233812-13
CAGAGTA126850.020.52550510-11
CATGGGG62500.019.5511074
AGTACTT96300.019.54552712-13
GTACTTT102300.018.56024614-15
Produced by FastQC (version 0.11.5)