##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1033088_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3156510 Sequences flagged as poor quality 0 Sequence length 100 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.72552724369636 34.0 31.0 34.0 31.0 34.0 2 32.719816189399054 34.0 31.0 34.0 31.0 34.0 3 32.82145850955644 34.0 31.0 34.0 31.0 34.0 4 36.31083158298247 37.0 37.0 37.0 35.0 37.0 5 36.29771361408644 37.0 37.0 37.0 35.0 37.0 6 36.3636877437423 37.0 37.0 37.0 35.0 37.0 7 36.31732863193844 37.0 37.0 37.0 35.0 37.0 8 36.32202052266585 37.0 37.0 37.0 35.0 37.0 9 38.122377245755594 39.0 39.0 39.0 37.0 39.0 10-11 38.08544024888247 39.0 39.0 39.0 36.0 39.0 12-13 38.062861356371435 39.0 39.0 39.0 35.0 39.0 14-15 39.56089732014155 41.0 39.5 41.0 37.0 41.0 16-17 39.56437457825257 41.0 40.0 41.0 37.0 41.0 18-19 39.58163161212859 41.0 40.0 41.0 37.0 41.0 20-21 39.57332750411055 41.0 40.0 41.0 37.0 41.0 22-23 39.50661363341158 41.0 39.0 41.0 37.0 41.0 24-25 39.45496165068383 41.0 39.0 41.0 36.0 41.0 26-27 39.35072184152751 41.0 39.0 41.0 36.0 41.0 28-29 39.30118675372484 41.0 39.0 41.0 36.0 41.0 30-31 39.29476478769274 41.0 39.0 41.0 36.0 41.0 32-33 39.25268239289595 41.0 39.0 41.0 35.5 41.0 34-35 39.1713515876712 41.0 39.0 41.0 35.0 41.0 36-37 39.08472696110578 41.0 39.0 41.0 35.0 41.0 38-39 38.99026187149732 41.0 39.0 41.0 35.0 41.0 40-41 38.88977351568663 41.0 39.0 41.0 35.0 41.0 42-43 38.7739134360417 41.0 39.0 41.0 35.0 41.0 44-45 38.6117756636285 41.0 39.0 41.0 35.0 41.0 46-47 38.41988018412741 41.0 38.0 41.0 35.0 41.0 48-49 38.221647325685645 40.5 38.0 41.0 34.0 41.0 50-51 38.09071062660976 40.0 38.0 41.0 34.0 41.0 52-53 37.95969884461003 40.0 38.0 41.0 34.0 41.0 54-55 37.814494330764035 40.0 38.0 41.0 33.5 41.0 56-57 37.66570436969945 40.0 37.0 41.0 33.0 41.0 58-59 37.49305055266734 40.0 37.0 41.0 33.0 41.0 60-61 37.30203199102806 40.0 36.5 41.0 33.0 41.0 62-63 37.07203192766695 39.5 36.0 41.0 33.0 41.0 64-65 36.80655898444802 39.0 35.5 41.0 32.0 41.0 66-67 36.529461018656676 39.0 35.0 41.0 32.0 41.0 68-69 36.22098837006694 38.0 35.0 40.5 32.0 41.0 70-71 35.89114354144293 37.0 35.0 40.0 31.0 41.0 72-73 35.53910363027521 37.0 35.0 39.5 31.0 41.0 74-75 35.18868576370738 36.0 35.0 39.0 31.0 41.0 76-77 34.830537840843206 36.0 35.0 39.0 31.0 40.5 78-79 34.46504319644164 35.5 35.0 37.5 30.5 39.5 80-81 34.12812536630646 35.0 34.0 37.0 30.0 39.0 82-83 33.824199194680205 35.0 34.0 37.0 30.0 39.0 84-85 33.598298278795255 35.0 34.0 36.0 30.0 37.5 86-87 33.38583815669838 35.0 34.0 36.0 30.0 37.0 88-89 33.17814389943324 35.0 34.0 36.0 30.0 37.0 90-91 32.994014908870874 35.0 34.0 35.0 30.0 36.0 92-93 32.82109212389633 35.0 34.0 35.0 29.5 36.0 94-95 32.66859046858714 35.0 34.0 35.0 29.0 36.0 96-97 32.527061849954535 35.0 34.0 35.0 29.0 36.0 98-99 32.4054097721851 35.0 34.0 35.0 29.0 35.5 100 32.301504826533105 35.0 34.0 35.0 29.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.11936930767129184 1101 2 0.05284699933015702 1101 3 0.11450519611251764 1101 4 0.09952140827554246 1101 5 0.10436149132330996 1101 6 0.10837385526004795 1101 7 0.13048763082483106 1101 8 0.13638657833249113 1101 9 0.18766738881603828 1101 10-11 0.19351336964421506 1101 12-13 0.21012019678828153 1101 14-15 0.2938899199605274 1101 16-17 0.30281801859259616 1101 18-19 0.27303892449430833 1101 20-21 0.2288027963225261 1101 22-23 0.32281932458108287 1101 24-25 0.2938236036184776 1101 26-27 0.2734561788944134 1101 28-29 0.29185916569979753 1101 30-31 0.31353002555147214 1101 32-33 0.28396878594072206 1101 34-35 0.2954271559322095 1101 36-37 0.3316709134827889 1101 38-39 0.2630439751073297 1101 40-41 0.2607215739588469 1101 42-43 0.20203119365663014 1101 44-45 0.24236026107780617 1101 46-47 0.2617719006856518 1101 48-49 0.23475857570210223 1101 50-51 0.2791670583476389 1101 52-53 0.32865702941504793 1101 54-55 0.31086800808773063 1101 56-57 0.26945512663621685 1101 58-59 0.22358245825121514 1101 60-61 0.197923163293531 1101 62-63 0.19229829666301157 1101 64-65 0.22090426463678625 1101 66-67 0.22284801856469727 1101 68-69 0.18402363837369506 1101 70-71 0.18267271353236936 1101 72-73 0.17385651980448813 1101 74-75 0.1885559709152531 1101 76-77 0.18738582431233652 1101 78-79 0.17918471521328172 1101 80-81 0.17088469636353665 1101 82-83 0.18443622975008367 1101 84-85 0.2076441389972743 1101 86-87 0.1722281566977557 1101 88-89 0.1747413343801938 1101 90-91 0.18990637088791118 1101 92-93 0.1683015885957886 1101 94-95 0.17751496727856875 1101 96-97 0.1864188193101981 1101 98-99 0.19550844256463762 1101 100 0.20093375767707755 1102 1 0.15086958010076756 1102 2 0.14383669736025695 1102 3 0.15609011895272573 1102 4 0.08864713021893067 1102 5 0.12011168042670306 1102 6 0.11641668618675283 1102 7 0.12643641260337546 1102 8 0.17638626946273206 1102 9 0.22821289088435748 1102 10-11 0.19733912352177896 1102 12-13 0.2386488407030285 1102 14-15 0.31369307261105206 1102 16-17 0.28685196443651506 1102 18-19 0.28906416105733257 1102 20-21 0.2983160503785811 1102 22-23 0.32110200345078255 1102 24-25 0.3580667939130251 1102 26-27 0.31714334650903453 1102 28-29 0.2806434649192724 1102 30-31 0.32392624303936657 1102 32-33 0.30090444784173087 1102 34-35 0.27500266734171674 1102 36-37 0.28056032044545276 1102 38-39 0.257414903143534 1102 40-41 0.2505335526684078 1102 42-43 0.20106787966185635 1102 44-45 0.23750103640225007 1102 46-47 0.23700252960687607 1102 48-49 0.20224003796346324 1102 50-51 0.16328523466636113 1102 52-53 0.16849676938056035 1102 54-55 0.17830729693415037 1102 56-57 0.14047214800417862 1102 58-59 0.18403245292532233 1102 60-61 0.18403560690497045 1102 62-63 0.1504408121300358 1102 64-65 0.24639366188908696 1102 66-67 0.19191452742571613 1102 68-69 0.2152712881340122 1102 70-71 0.15877190641973726 1102 72-73 0.14497875786628356 1102 74-75 0.14766526458407014 1102 76-77 0.18803976610921325 1102 78-79 0.16588477824598158 1102 80-81 0.22145672420878526 1102 82-83 0.23429441939462947 1102 84-85 0.2528507712771457 1102 86-87 0.21218931844153133 1102 88-89 0.2922982740436453 1102 90-91 0.23907259179134854 1102 92-93 0.297750727848765 1102 94-95 0.23764061697605143 1102 96-97 0.21173833819690202 1102 98-99 0.24116669108483535 1102 100 0.26128595593591797 1103 1 0.15859415236376861 1103 2 0.0966400196904047 1103 3 0.13578461743550463 1103 4 0.12036665054735352 1103 5 0.11969881147244621 1103 6 0.1217564818939465 1103 7 0.1555958074179955 1103 8 0.1743237171019416 1103 9 0.19829709884145785 1103 10-11 0.22687397712305568 1103 12-13 0.2254801032303817 1103 14-15 0.28392218415362436 1103 16-17 0.3097305992465422 1103 18-19 0.29778057646986866 1103 20-21 0.2764973703168394 1103 22-23 0.2506351773073803 1103 24-25 0.2633534933002295 1103 26-27 0.27195240178733826 1103 28-29 0.29627989073598826 1103 30-31 0.25172661685425624 1103 32-33 0.2090058718504153 1103 34-35 0.2410112667127251 1103 36-37 0.235179815511124 1103 38-39 0.19539061208925546 1103 40-41 0.1315433578195595 1103 42-43 0.19905518191865212 1103 44-45 0.2046957506144338 1103 46-47 0.19270356516435783 1103 48-49 0.12044691869958513 1103 50-51 0.13694023159698077 1103 52-53 0.13896091715056258 1103 54-55 0.18002250574715362 1103 56-57 0.2010604109827625 1103 58-59 0.21223539189472262 1103 60-61 0.22075490266995956 1103 62-63 0.17982123676436856 1103 64-65 0.1919469398314817 1103 66-67 0.18948148381591068 1103 68-69 0.20185294469720105 1103 70-71 0.1632154968801558 1103 72-73 0.19371493690903208 1103 74-75 0.1631463652285774 1103 76-77 0.20206955197709675 1103 78-79 0.16920071205318976 1103 80-81 0.16649365989970732 1103 82-83 0.16960865481607357 1103 84-85 0.2360646933698689 1103 86-87 0.18515741245930428 1103 88-89 0.17633036495143273 1103 90-91 0.20158729659900132 1103 92-93 0.19491563120173794 1103 94-95 0.21100391190178414 1103 96-97 0.18659368682919109 1103 98-99 0.19870540830375916 1103 100 0.16195947722414417 1104 1 0.08497453515420261 1104 2 0.14094306641224108 1104 3 0.10732471584979209 1104 4 0.0566894364299273 1104 5 0.07786323315471577 1104 6 0.09224754212288389 1104 7 0.08927478638673847 1104 8 0.0827879997807841 1104 9 0.13190051812727432 1104 10-11 0.15102432290635903 1104 12-13 0.12631344442432635 1104 14-15 0.19511194271795063 1104 16-17 0.20426770748169076 1104 18-19 0.24178794339233178 1104 20-21 0.245451724497336 1104 22-23 0.2195757244953569 1104 24-25 0.20482017810252273 1104 26-27 0.23504369601668884 1104 28-29 0.20370517890014384 1104 30-31 0.19221161227008565 1104 32-33 0.21129922552588454 1104 34-35 0.20920738485111912 1104 36-37 0.20741825450085827 1104 38-39 0.201512363762113 1104 40-41 0.22791667876097677 1104 42-43 0.16668638634732957 1104 44-45 0.16519194117966407 1104 46-47 0.1142595159610309 1104 48-49 0.15051160824728527 1104 50-51 0.21129961035954636 1104 52-53 0.22923416217551562 1104 54-55 0.18884375660984887 1104 56-57 0.20839434328705408 1104 58-59 0.21526354638073997 1104 60-61 0.2725957364422271 1104 62-63 0.28643414103861176 1104 64-65 0.22396002817614402 1104 66-67 0.17980867141657342 1104 68-69 0.23439372896467603 1104 70-71 0.2653470912525222 1104 72-73 0.2795390068907935 1104 74-75 0.23431310301551633 1104 76-77 0.293742703857383 1104 78-79 0.27682203996134547 1104 80-81 0.29139743249301375 1104 82-83 0.24861935276712188 1104 84-85 0.29361824971635286 1104 86-87 0.28852254553646617 1104 88-89 0.283596938681967 1104 90-91 0.2751812303587755 1104 92-93 0.22376598964837058 1104 94-95 0.21722106622735282 1104 96-97 0.2146032605244912 1104 98-99 0.20724037789685212 1104 100 0.2015395550193375 1105 1 -0.0038682574092092636 1105 2 0.05609365083686413 1105 3 0.06859432167779289 1105 4 0.04797523855634722 1105 5 0.014451184715618126 1105 6 0.05488396229265646 1105 7 0.045747554442741034 1105 8 0.05519228728213932 1105 9 0.06962174041736091 1105 10-11 0.08003018356158975 1105 12-13 0.05270330938565593 1105 14-15 0.08930372191762359 1105 16-17 0.0781198955553819 1105 18-19 0.11656166746890761 1105 20-21 0.14188651390239215 1105 22-23 0.14940822805914422 1105 24-25 0.12797839438048442 1105 26-27 0.11175741013799012 1105 28-29 0.11644794124766378 1105 30-31 0.07331452186413401 1105 32-33 0.08346309856809597 1105 34-35 0.09511781554567023 1105 36-37 0.04757843646471116 1105 38-39 0.03700397772164621 1105 40-41 0.09954706035969707 1105 42-43 0.1136171033094655 1105 44-45 0.08902530192345637 1105 46-47 0.08447176600081718 1105 48-49 0.04003777539627151 1105 50-51 0.11953377532753962 1105 52-53 0.11838597059769285 1105 54-55 0.11343968356641909 1105 56-57 0.029054168930471747 1105 58-59 0.0776825712893725 1105 60-61 0.08785340552428522 1105 62-63 0.13570845907567275 1105 64-65 0.16832313863817205 1105 66-67 0.0873613610574111 1105 68-69 0.14781021598927424 1105 70-71 0.13626578628138475 1105 72-73 0.1471277386838068 1105 74-75 0.14047023341110076 1105 76-77 0.16019481328874718 1105 78-79 0.169955611034041 1105 80-81 0.21965927729018375 1105 82-83 0.1979612667140529 1105 84-85 0.17692422922885243 1105 86-87 0.11627314535066802 1105 88-89 0.11312185548583642 1105 90-91 0.11345760867224897 1105 92-93 0.22305006698747576 1105 94-95 0.1755439121320066 1105 96-97 0.1621350369097101 1105 98-99 0.13122635429419205 1105 100 0.142332874322058 1106 1 0.03551424706691364 1106 2 0.05808193267473882 1106 3 0.0736272723563971 1106 4 0.04820527062116042 1106 5 0.06750249807879527 1106 6 0.04596920929261472 1106 7 0.0698280762643293 1106 8 0.06354035840130479 1106 9 0.06334817947742977 1106 10-11 0.10726365366713964 1106 12-13 0.06743070589469369 1106 14-15 0.14578256693928893 1106 16-17 0.11087245106924826 1106 18-19 0.09505447779921639 1106 20-21 0.14507390861231073 1106 22-23 0.09364963669609949 1106 24-25 0.08942392898968876 1106 26-27 0.07974915790607895 1106 28-29 0.09278699665480161 1106 30-31 0.10097924595297059 1106 32-33 0.14284352727654692 1106 34-35 0.08896715244747355 1106 36-37 0.04079350104098012 1106 38-39 0.07767624710472631 1106 40-41 0.08802392248879443 1106 42-43 0.06507696933846319 1106 44-45 0.10312287225580974 1106 46-47 0.16524050603543827 1106 48-49 0.20356012409820323 1106 50-51 0.19986427778143678 1106 52-53 0.19814972380481066 1106 54-55 0.2448539213296641 1106 56-57 0.23070448956292466 1106 58-59 0.19618377810441245 1106 60-61 0.2125189956241229 1106 62-63 0.21967326156493527 1106 64-65 0.24941507776581773 1106 66-67 0.24535166764452043 1106 68-69 0.2643315167923035 1106 70-71 0.23072291622223418 1106 72-73 0.22503769482815983 1106 74-75 0.23168708641706104 1106 76-77 0.23375387595254438 1106 78-79 0.2063880732919614 1106 80-81 0.10833761283708299 1106 82-83 0.16026133765932116 1106 84-85 0.1492919754997999 1106 86-87 0.10107095403701294 1106 88-89 0.19025115322201458 1106 90-91 0.1181970461607591 1106 92-93 0.13192418039058396 1106 94-95 0.1423773258478107 1106 96-97 0.16796117605234429 1106 98-99 0.17279366936953977 1106 100 0.1679733656421618 1107 1 0.027151028116094267 1107 2 0.03807167443010684 1107 3 0.05198992254683077 1107 4 0.02923141543989516 1107 5 0.03515459351542205 1107 6 0.024551483224065862 1107 7 0.06385352246713438 1107 8 0.04320558980455047 1107 9 0.06079509631643987 1107 10-11 0.06371675851640646 1107 12-13 0.06537147635259544 1107 14-15 0.06891954688328639 1107 16-17 0.06193567324076099 1107 18-19 0.1214720274149883 1107 20-21 0.0591139558536824 1107 22-23 0.023994241250022696 1107 24-25 0.06046790067775021 1107 26-27 0.05992029910667185 1107 28-29 0.06888265701302743 1107 30-31 0.045480180981350316 1107 32-33 0.016769992040131854 1107 34-35 0.05954187539949629 1107 36-37 -0.005312944350485793 1107 38-39 -0.08718353461463124 1107 40-41 -0.03919073110871807 1107 42-43 0.03296605540904096 1107 44-45 0.04717993434114476 1107 46-47 0.046614840996930695 1107 48-49 -0.05297438886474026 1107 50-51 0.007411287026876323 1107 52-53 0.08817720418257835 1107 54-55 2.3337530598865897E-4 1107 56-57 -0.0033964518725753123 1107 58-59 0.008600712533876731 1107 60-61 0.02168480915020865 1107 62-63 0.023433517239865864 1107 64-65 0.05763202936088163 1107 66-67 0.018283461281811242 1107 68-69 0.0019373584754518447 1107 70-71 0.005133829500664433 1107 72-73 0.024753220410730137 1107 74-75 0.03977270489944118 1107 76-77 0.06375347250956054 1107 78-79 0.06387806944768926 1107 80-81 0.021059981874863354 1107 82-83 -0.015908317226468682 1107 84-85 0.0640848593479646 1107 86-87 0.0763623409489469 1107 88-89 0.0025986200797305514 1107 90-91 -0.016445905082079548 1107 92-93 0.006513071129660375 1107 94-95 0.018584314199877383 1107 96-97 0.051176423079375866 1107 98-99 0.06917210043372535 1107 100 -0.01055019919720479 1108 1 0.0342411530258957 1108 2 0.03866063915334195 1108 3 0.043942504068745336 1108 4 0.004101309271810294 1108 5 0.009972039094094498 1108 6 0.05141644782375465 1108 7 0.03851522507297034 1108 8 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50-51 -0.10807703169221128 2214 52-53 -0.18017881812437508 2214 54-55 -0.16537307424154335 2214 56-57 -0.14849185802483333 2214 58-59 -0.16123522530082823 2214 60-61 -0.10291889249068475 2214 62-63 -0.07638301625627264 2214 64-65 -0.13979194204851098 2214 66-67 -0.08935185735159479 2214 68-69 -0.14726637041838586 2214 70-71 -0.13159371597248537 2214 72-73 -0.1793566494415586 2214 74-75 -0.2325444414905533 2214 76-77 -0.1621631459586581 2214 78-79 -0.135999783186449 2214 80-81 -0.16640855524865117 2214 82-83 -0.15167449702929048 2214 84-85 -0.11066858475849983 2214 86-87 -0.14152707871792813 2214 88-89 -0.14614930619687527 2214 90-91 -0.12250696418647067 2214 92-93 -0.22212802417712396 2214 94-95 -0.24617422942150569 2214 96-97 -0.1868736342827333 2214 98-99 -0.1891887684950504 2214 100 -0.2178364063481908 2215 1 -0.19361514359188448 2215 2 -0.12275197492888168 2215 3 -0.18978656344638978 2215 4 -0.07726180107425051 2215 5 -0.12453132115271615 2215 6 -0.13366471047423545 2215 7 -0.1371154041463356 2215 8 -0.12787567855502147 2215 9 -0.18533983960587364 2215 10-11 -0.14311278795373283 2215 12-13 -0.18721775203397328 2215 14-15 -0.2555217063182411 2215 16-17 -0.24925402094085314 2215 18-19 -0.26788195095218015 2215 20-21 -0.3036309216943138 2215 22-23 -0.24872043341077443 2215 24-25 -0.30853760454700563 2215 26-27 -0.31394068000369657 2215 28-29 -0.3303466085807045 2215 30-31 -0.3431968158459284 2215 32-33 -0.24273391447629677 2215 34-35 -0.2543659977833599 2215 36-37 -0.24968531021539064 2215 38-39 -0.2734344518581011 2215 40-41 -0.1995073976577899 2215 42-43 -0.20586066230575284 2215 44-45 -0.23523587623162 2215 46-47 -0.26173827039875874 2215 48-49 -0.12253499543314206 2215 50-51 -0.17127858207777535 2215 52-53 -0.10418837555057081 2215 54-55 -0.12878919299925684 2215 56-57 -0.03298979109742817 2215 58-59 -0.03899595522642585 2215 60-61 -0.06947684442810242 2215 62-63 -0.07315872482068642 2215 64-65 -0.07082725737648587 2215 66-67 -0.054799161775811456 2215 68-69 -0.0996142455834601 2215 70-71 -0.1407973284369035 2215 72-73 -0.06560841752740032 2215 74-75 -0.10821596684969137 2215 76-77 -0.12630389489584104 2215 78-79 -0.15106173203965767 2215 80-81 -0.18941663635428796 2215 82-83 -0.13717756904978273 2215 84-85 -0.1281825282955893 2215 86-87 -0.1402630791603201 2215 88-89 -0.14383382464459515 2215 90-91 -0.18435992732405992 2215 92-93 -0.11989711721099638 2215 94-95 -0.12116698934421066 2215 96-97 -0.13145395484845324 2215 98-99 -0.1628299730656977 2215 100 -0.15697716838958087 2216 1 -0.2032928060440753 2216 2 -0.1870936929216498 2216 3 -0.13577830723504292 2216 4 -0.13448472362617991 2216 5 -0.18132898786894458 2216 6 -0.17483183435999905 2216 7 -0.19048737051656417 2216 8 -0.16102555044271583 2216 9 -0.22470966783821922 2216 10-11 -0.25376763986141526 2216 12-13 -0.30501447923445824 2216 14-15 -0.37124921640515396 2216 16-17 -0.3419199453118793 2216 18-19 -0.35903824898472436 2216 20-21 -0.3539131525800485 2216 22-23 -0.37172466378186186 2216 24-25 -0.3629094321831019 2216 26-27 -0.3809670246983572 2216 28-29 -0.4397277650674525 2216 30-31 -0.36330446741615674 2216 32-33 -0.3718777361274448 2216 34-35 -0.37899044013716576 2216 36-37 -0.32327720334725285 2216 38-39 -0.3339130999347262 2216 40-41 -0.3289035037679824 2216 42-43 -0.29332070929683596 2216 44-45 -0.2935481741713417 2216 46-47 -0.18146291118828373 2216 48-49 -0.20855873605742659 2216 50-51 -0.27013886455986125 2216 52-53 -0.2618478962190238 2216 54-55 -0.24375975407428285 2216 56-57 -0.23989760353805423 2216 58-59 -0.2917957261620785 2216 60-61 -0.2613591313175547 2216 62-63 -0.3140746034783035 2216 64-65 -0.4122548711824692 2216 66-67 -0.3485982813750965 2216 68-69 -0.3359374249720588 2216 70-71 -0.33112542319639715 2216 72-73 -0.36750945775290234 2216 74-75 -0.3957623273865565 2216 76-77 -0.44561119203095956 2216 78-79 -0.4895894772095346 2216 80-81 -0.43396969565879573 2216 82-83 -0.3917732967524188 2216 84-85 -0.48191039618397014 2216 86-87 -0.4864231145987361 2216 88-89 -0.5740744939449982 2216 90-91 -0.46600763238344456 2216 92-93 -0.4705003748109675 2216 94-95 -0.46341722135410635 2216 96-97 -0.5608845659748845 2216 98-99 -0.6253227908962984 2216 100 -0.6315907338849769 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 15.0 3 6.0 4 8.0 5 32.0 6 68.0 7 180.0 8 370.0 9 743.0 10 1330.0 11 2103.0 12 3467.0 13 5287.0 14 6272.0 15 8225.0 16 10204.0 17 14368.0 18 16504.0 19 8692.0 20 5895.0 21 5447.0 22 5834.0 23 6111.0 24 7079.0 25 8786.0 26 11029.0 27 14318.0 28 18414.0 29 24712.0 30 31433.0 31 40294.0 32 51837.0 33 68514.0 34 94725.0 35 143436.0 36 260043.0 37 599678.0 38 1128229.0 39 550807.0 40 2015.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.45104604870943 20.089161909114566 13.788433483066939 23.671358559109066 2 15.493496326150789 20.74820436351972 40.11278010420487 23.645519206124618 3 17.823757406329065 25.948621572147196 30.668902954041 25.55871806748274 4 12.0665533746203 17.350220691385623 39.594524724658505 30.988701209335566 5 12.369701533627563 37.89985309692403 36.28002254393549 13.450422825512916 6 30.118612011366984 38.97545073514736 17.8346971813807 13.07124007210495 7 26.30208679839443 32.994826564781995 23.187792847163482 17.5152937896601 8 24.865175163780933 36.09925287572988 20.797002252175904 18.238569708313282 9 25.00856959109903 16.214996942826097 21.51147945040567 37.264954015669204 10-11 23.530949215512 26.716406585742273 29.466189532630516 20.28645466611521 12-13 24.723698008243282 24.16949415652097 29.445685266322613 21.661122568913136 14-15 21.78873407433327 25.896019975285924 27.04941754325716 25.265828407123646 16-17 20.88853610787749 29.338368224491223 27.45712704852659 22.315968619104698 18-19 20.738379587726 27.895658251103363 30.254304381476533 21.11165777969411 20-21 21.989824204580373 27.215706587338552 29.753572774995167 21.04089643308591 22-23 21.50734292452292 27.108041544643633 29.854367547935883 21.530247982897556 24-25 21.430632953901036 27.29389159409087 29.781734025667916 21.493741426340186 26-27 21.182389454924298 27.197599624775048 29.93711560549614 21.682895314804515 28-29 21.174151936345943 27.260514376764515 29.995537778478976 21.569795908410565 30-31 21.531173540879205 27.747095127620497 29.45740338925274 21.264327942247554 32-33 21.1357005046713 27.357239482846563 29.980738220376303 21.526321792105836 34-35 21.272655559462823 27.23975213130958 29.962759503375562 21.524832805852032 36-37 21.67582234809964 27.506407392975152 29.300493266297273 21.517276992627934 38-39 21.53897817526319 27.723862747147958 29.361494181865417 21.375664895723443 40-41 21.39891614404272 27.45426274858166 29.520422568230465 21.626398539145157 42-43 21.374302631704005 27.402083313532984 29.377239419485445 21.84637463527757 44-45 21.537916876550366 27.570164517140768 29.229766419241503 21.662152187067363 46-47 21.677707341335843 27.80189196295909 28.887521344776353 21.632879350928715 48-49 21.696715676490808 27.752137645690965 28.625602326620225 21.925544351198 50-51 21.781667094354205 27.745706492296872 28.441997649302554 22.030628764046366 52-53 21.771038266946725 27.734713338465582 28.486255389655028 22.007993004932665 54-55 21.836252696807552 28.112044631570942 28.272205695530822 21.77949697609068 56-57 21.66612809717061 27.80540850496276 28.387982296903857 22.140481100962774 58-59 21.529394806289222 27.383249221450274 28.812961150131 22.274394822129505 60-61 21.75427925145176 27.790819607731322 28.47629185397797 21.978609286838946 62-63 21.656322964286506 27.790851288289915 28.423607085040125 22.129218662383455 64-65 21.64094205309028 27.50517185119008 28.55641198665615 22.297474109063494 66-67 21.515978089725678 27.630896147960883 28.409509236466857 22.443616525846583 68-69 21.993547923128855 27.738513158390788 28.308320948847506 21.95961796963285 70-71 21.91067032893924 27.579684524997543 28.33338085417122 22.176264291891997 72-73 21.75109884435219 27.71501835261736 28.439111952549943 22.0947708504805 74-75 21.850640597343837 27.765279785535085 28.202329346964856 22.18175027015622 76-77 21.82912457112444 27.80506001881825 28.059850911291267 22.30596449876604 78-79 22.009355268952103 27.845183446274525 28.128201716452665 22.017259568320707 80-81 21.887131673905674 27.656652442095858 28.281345536684505 22.174870347313963 82-83 21.7354341365313 27.767006377619314 28.344305905230055 22.153253580619335 84-85 21.723929276320998 27.668532651567713 28.214277794146064 22.393260277965222 86-87 21.79760750490536 27.818968853274146 28.12036323194505 22.263060409875447 88-89 21.552843635303752 27.73274279368972 28.235921221251193 22.478492349755342 90-91 21.553276931121534 27.57432313306477 28.28948087503107 22.58291906078263 92-93 21.682409388924707 27.76213097797075 28.112394007891687 22.443065625212853 94-95 21.48013070841471 27.54536167767282 28.281352888167135 22.69315472574534 96-97 21.370759965651878 27.60208325380179 28.25460885609382 22.772547924452507 98-99 21.303652643997733 27.73896026875268 28.09341842314355 22.863968664106032 100 21.534384494267403 27.59243594983067 27.985940168097045 22.887239387804886 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 18.0 1 20.5 2 102.5 3 183.5 4 128.5 5 92.5 6 232.0 7 521.0 8 916.0 9 1142.5 10 979.0 11 808.5 12 878.5 13 1065.5 14 1271.0 15 1514.5 16 1762.5 17 1971.0 18 2308.0 19 2759.5 20 3416.0 21 4375.5 22 5638.0 23 7623.0 24 10242.0 25 13588.5 26 18158.5 27 24083.5 28 31684.5 29 40294.0 30 48754.0 31 57939.0 32 68012.5 33 78361.5 34 88320.5 35 96019.0 36 102044.5 37 108252.5 38 113728.5 39 116513.0 40 117820.5 41 118398.5 42 117687.5 43 117145.5 44 114928.0 45 111760.0 46 109384.5 47 106233.0 48 103377.0 49 101215.0 50 98232.5 51 94171.5 52 88809.5 53 84311.0 54 81092.0 55 76492.5 56 69487.5 57 62782.5 58 56786.5 59 50016.5 60 43392.0 61 36636.0 62 30694.0 63 25320.5 64 20359.0 65 16158.5 66 12477.5 67 9293.5 68 7057.0 69 5587.0 70 4173.5 71 2962.5 72 2188.5 73 1610.0 74 1108.0 75 665.0 76 396.0 77 243.5 78 153.5 79 91.0 80 49.5 81 23.5 82 10.0 83 8.0 84 4.0 85 1.0 86 1.0 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.042800434657263874 2 0.2504348156666698 3 0.0027878891560616 4 0.0020909168670461995 5 2.2176391014126358E-4 6 0.0 7 0.0 8 2.2176391014126358E-4 9 0.0 10-11 7.761736854944225E-4 12-13 0.0 14-15 3.0096530662028633E-4 16-17 1.1088195507063179E-4 18-19 5.385694960573545E-4 20-21 0.0 22-23 6.336111718321817E-5 24-25 0.0012672223436643634 26-27 1.1088195507063179E-4 28-29 3.326458652118954E-4 30-31 9.504167577482726E-5 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 6.336111718321817E-5 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 2.2176391014126358E-4 70-71 0.0 72-73 3.8016670309930906E-4 74-75 9.504167577482726E-5 76-77 0.0 78-79 0.0 80-81 0.0 82-83 9.504167577482726E-5 84-85 0.0 86-87 4.593680995783318E-4 88-89 0.0023760418943706814 90-91 0.0023285210564832678 92-93 7.920139647902271E-4 94-95 5.860903339447682E-4 96-97 1.7424307225385E-4 98-99 6.336111718321817E-5 100 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 3156510.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.05678789069615 #Duplication Level Percentage of deduplicated Percentage of total 1 73.4388799722749 44.83942117397483 2 15.505549450186104 18.934380878174267 3 5.174619767998554 9.47836984769073 4 2.2727067332106254 5.550566917895924 5 1.1703896134166718 3.57301151879278 6 0.6686328582831049 2.449474476296486 7 0.42438016709368637 1.8137902893080176 8 0.29019351571986923 1.4174627149250765 9 0.2001616669170473 1.0999105596722096 >10 0.8166253708414389 8.273826051038382 >50 0.028676763786387848 1.1869817613186853 >100 0.0087690027382019 0.9005376826366502 >500 2.0755876679067313E-4 0.07781143161425787 >1k 1.5566907493376114E-4 0.2425270799737971 >5k 5.1889691644587046E-5 0.16192761668792646 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5111 0.16191933496171404 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4368 0.1383806799281485 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 3.1680558591609086E-5 0.0 6 0.0 0.0 0.0 3.1680558591609086E-5 0.0 7 0.0 0.0 0.0 3.1680558591609086E-5 0.0 8 0.0 0.0 0.0 3.1680558591609086E-5 0.0 9 0.0 0.0 0.0 9.504167577482726E-5 0.0 10-11 0.0 0.0 0.0 1.2672223436643634E-4 0.0 12-13 0.0 1.5840279295804543E-5 0.0 1.2672223436643634E-4 0.0 14-15 0.0 3.1680558591609086E-5 0.0 1.2672223436643634E-4 0.0 16-17 0.0 3.1680558591609086E-5 0.0 1.7424307225384996E-4 0.0 18-19 0.0 3.1680558591609086E-5 0.0 1.9008335154965453E-4 0.0 20-21 0.0 4.752083788741363E-5 0.0 2.217639101412636E-4 0.0 22-23 0.0 6.336111718321817E-5 0.0 3.168055859160909E-4 0.0 24-25 0.0 6.336111718321817E-5 0.0 4.5936809957833173E-4 0.0 26-27 0.0 6.336111718321817E-5 0.0 5.860903339447682E-4 0.0 28-29 0.0 7.920139647902272E-5 0.0 0.0010612987128189044 0.0 30-31 0.0 9.504167577482726E-5 0.0 0.003199736417752518 3.1680558591609086E-5 32-33 0.0 9.504167577482726E-5 0.0 0.009092320315791809 6.336111718321817E-5 34-35 0.0 9.504167577482726E-5 0.0 0.017551029459751434 6.336111718321817E-5 36-37 0.0 1.108819550706318E-4 0.0 0.034452607468374884 6.336111718321817E-5 38-39 0.0 1.2672223436643634E-4 0.0 0.060668269702931404 6.336111718321817E-5 40-41 0.0 1.2672223436643634E-4 0.0 0.09255475192538595 6.336111718321817E-5 42-43 0.0 1.2672223436643634E-4 0.0 0.12808449838587554 6.336111718321817E-5 44-45 0.0 1.2672223436643634E-4 0.0 0.1677168771839785 6.336111718321817E-5 46-47 0.0 1.2672223436643634E-4 0.0 0.2178672014344957 9.504167577482726E-5 48-49 0.0 1.2672223436643634E-4 0.0 0.2730072136631913 9.504167577482726E-5 50-51 0.0 1.2672223436643634E-4 0.0 0.3335646014110521 9.504167577482726E-5 52-53 0.0 1.2672223436643634E-4 0.0 0.39689403803567863 9.504167577482726E-5 54-55 0.0 1.2672223436643634E-4 0.0 0.46592597520679485 9.504167577482726E-5 56-57 0.0 1.5840279295804542E-4 0.0 0.549230004023431 9.504167577482726E-5 58-59 0.0 2.217639101412636E-4 0.0 0.646631881413333 9.504167577482726E-5 60-61 0.0 2.217639101412636E-4 0.0 0.7499421829805704 9.504167577482726E-5 62-63 0.0 2.217639101412636E-4 0.0 0.8558978111902069 9.504167577482726E-5 64-65 0.0 2.217639101412636E-4 0.0 0.9701537457508451 9.504167577482726E-5 66-67 0.0 2.217639101412636E-4 0.0 1.100376681841654 9.504167577482726E-5 68-69 0.0 2.534444687328727E-4 0.0 1.2466933416970007 9.504167577482726E-5 70-71 0.0 2.534444687328727E-4 0.0 1.4108461560394234 9.504167577482726E-5 72-73 0.0 2.534444687328727E-4 0.0 1.578626394340585 9.504167577482726E-5 74-75 0.0 2.534444687328727E-4 0.0 1.751824008160912 9.504167577482726E-5 76-77 0.0 2.534444687328727E-4 0.0 1.9385492204998558 9.504167577482726E-5 78-79 0.0 2.8512502732448177E-4 0.0 2.159790401424358 9.504167577482726E-5 80-81 0.0 3.009653066202863E-4 0.0 2.4040791887242556 9.504167577482726E-5 82-83 0.0 3.1680558591609085E-4 0.0 2.6589334423144546 9.504167577482726E-5 84-85 0.0 3.1680558591609085E-4 0.0 2.9110790081450717 9.504167577482726E-5 86-87 0.0 3.484861445077E-4 0.0 3.1854168052691105 9.504167577482726E-5 88 0.0 3.484861445077E-4 0.0 3.4099052434492525 9.504167577482726E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4220 0.0 53.298782 1 GTATCAA 7615 0.0 44.521557 1 ATCAACG 9135 0.0 36.477295 3 TCAACGC 9235 0.0 36.030838 4 CAACGCA 9440 0.0 35.149376 5 TATCAAC 9785 0.0 34.197544 2 AACGCAG 9795 0.0 33.875458 6 ACGCAGA 11075 0.0 29.917843 7 CGCAGAG 11315 0.0 29.20019 8 GTACATG 7955 0.0 27.385023 1 TACATGG 7920 0.0 26.540377 2 GCAGAGT 12785 0.0 25.327744 9 ACATGGG 8480 0.0 23.66557 3 GTGGTAT 2135 0.0 22.085815 1 AGAGTAC 11750 0.0 21.638842 10-11 GAGTACT 8975 0.0 21.312338 12-13 CAGAGTA 12685 0.0 20.525505 10-11 CATGGGG 6250 0.0 19.551107 4 AGTACTT 9630 0.0 19.545527 12-13 GTACTTT 10230 0.0 18.560246 14-15 >>END_MODULE