FastQCFastQC Report
Wed 25 May 2016
SRR1033087_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033087_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2273091
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA28500.035.9661331
TCAACGC28150.035.241754
ATCAACG28850.034.5496373
CAACGCA29350.034.121255
AACGCAG30750.032.554146
GGTATCA17900.031.5217511
TATCAAC35200.028.1833882
GTACATG60550.027.179161
ACGCAGA38150.025.7462467
TACATGG61100.025.4706612
CGCAGAG38350.025.0245918
ACATGGG60950.024.2219643
TACACCG1802.1553751E-723.5084195
CATGGGG42450.021.3763264
GTATAGA10850.019.501361
GCAGAGT47150.019.4607879
GAGTACA44300.019.105171
GTCTAGG7150.017.7557831
ACCGGTC1902.0096135E-417.3319198
ACCGTCC2253.8454255E-516.7266778
Produced by FastQC (version 0.11.5)