##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1033087_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2273091 Sequences flagged as poor quality 0 Sequence length 100 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.577466102324983 33.0 31.0 34.0 30.0 34.0 2 31.63169886291398 33.0 31.0 34.0 30.0 34.0 3 31.789503367881004 34.0 31.0 34.0 30.0 34.0 4 35.15728406825772 37.0 35.0 37.0 33.0 37.0 5 35.19289328935797 37.0 35.0 37.0 33.0 37.0 6 35.332046099342264 37.0 35.0 37.0 33.0 37.0 7 35.20200115173568 37.0 35.0 37.0 33.0 37.0 8 35.21626806845833 37.0 35.0 37.0 33.0 37.0 9 36.865565874837394 39.0 38.0 39.0 34.0 39.0 10-11 36.862091530871396 39.0 38.0 39.0 34.0 39.0 12-13 36.79869459691671 39.0 38.0 39.0 33.5 39.0 14-15 38.19559665671106 41.0 39.0 41.0 34.0 41.0 16-17 38.194449540295565 41.0 39.0 41.0 33.5 41.0 18-19 38.19738145107257 41.0 38.5 41.0 33.5 41.0 20-21 38.24560499337686 41.0 39.0 41.0 34.0 41.0 22-23 38.201544064887855 40.0 39.0 41.0 34.0 41.0 24-25 38.213557002337346 40.0 38.5 41.0 34.0 41.0 26-27 38.13717158705921 40.0 38.0 41.0 33.0 41.0 28-29 38.14587493417554 40.0 38.0 41.0 33.0 41.0 30-31 38.286297380967156 40.5 39.0 41.0 34.0 41.0 32-33 38.27714266608771 41.0 39.0 41.0 33.5 41.0 34-35 38.235297223032426 41.0 39.0 41.0 33.0 41.0 36-37 38.156444242663405 41.0 38.0 41.0 33.0 41.0 38-39 38.06625361677117 40.5 38.0 41.0 33.0 41.0 40-41 37.996860002525196 40.0 38.0 41.0 33.0 41.0 42-43 37.875470449709226 40.0 38.0 41.0 33.0 41.0 44-45 37.730017188049224 40.0 38.0 41.0 32.5 41.0 46-47 37.60080348740988 40.0 38.0 41.0 32.0 41.0 48-49 37.48793339114008 40.0 38.0 41.0 32.0 41.0 50-51 37.36411894640382 40.0 37.0 41.0 31.5 41.0 52-53 37.21417268380368 40.0 37.0 41.0 31.0 41.0 54-55 37.06499343845012 40.0 37.0 41.0 31.0 41.0 56-57 36.89574724461097 40.0 36.0 41.0 31.0 41.0 58-59 36.7041462484344 40.0 36.0 41.0 30.5 41.0 60-61 36.488654215779306 39.0 35.0 41.0 30.0 41.0 62-63 36.242206977195366 39.0 35.0 41.0 30.0 41.0 64-65 35.975897577351724 39.0 35.0 41.0 30.0 41.0 66-67 35.68352762823838 38.0 35.0 40.0 29.5 41.0 68-69 35.3718115552787 37.5 35.0 40.0 29.0 41.0 70-71 35.0365788259247 37.0 34.5 40.0 29.0 41.0 72-73 34.68693114353979 36.5 34.0 39.0 29.0 41.0 74-75 34.33354251985512 36.0 34.0 39.0 29.0 41.0 76-77 33.97037866059916 35.5 34.0 38.5 28.0 40.0 78-79 33.58507996380259 35.0 34.0 37.0 28.0 39.5 80-81 33.229389188554265 35.0 34.0 37.0 27.0 39.0 82-83 32.99975605904031 35.0 34.0 36.5 27.5 39.0 84-85 32.77047003397576 35.0 34.0 36.0 27.0 37.5 86-87 32.59030896695293 35.0 34.0 36.0 27.0 37.0 88-89 32.364167778588715 35.0 34.0 35.5 27.0 37.0 90-91 32.15578302848412 35.0 33.0 35.0 27.0 36.0 92-93 31.939160156808505 35.0 33.0 35.0 26.5 36.0 94-95 31.743558440907115 35.0 33.0 35.0 26.0 36.0 96-97 31.572408231786582 35.0 33.0 35.0 25.5 36.0 98-99 31.398206011109984 35.0 33.0 35.0 25.0 35.0 100 31.262776105312106 35.0 33.0 35.0 25.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.13120145550041684 1101 2 -0.06474436674270123 1101 3 -0.05548440426077761 1101 4 -0.13489205570245133 1101 5 -0.12226140607008773 1101 6 0.02690512644036147 1101 7 0.09912772269828451 1101 8 0.1364850572441867 1101 9 0.21076473041930655 1101 10-11 0.13740238287241624 1101 12-13 0.14129267239582788 1101 14-15 0.16802037723702767 1101 16-17 0.23752509604201322 1101 18-19 0.20963281014697088 1101 20-21 0.18905222029673752 1101 22-23 0.18995330154381662 1101 24-25 0.18012266175529135 1101 26-27 0.10188594602320222 1101 28-29 0.16438785968511382 1101 30-31 0.1821538770604647 1101 32-33 0.24570536910318452 1101 34-35 0.19996139111503908 1101 36-37 0.18637029455742038 1101 38-39 0.2346892703002581 1101 40-41 0.17253515342706294 1101 42-43 0.21066126053028 1101 44-45 0.17769839503073115 1101 46-47 0.19001130661278154 1101 48-49 0.2073813977866621 1101 50-51 0.22794512004507084 1101 52-53 0.07963205393076578 1101 54-55 0.13322514123973406 1101 56-57 0.1960633344173459 1101 58-59 0.24773744811281517 1101 60-61 0.2694437314914424 1101 62-63 0.17877211326915443 1101 64-65 0.15710101495312756 1101 66-67 0.11455131783928607 1101 68-69 0.23421269473272588 1101 70-71 0.10078479168588217 1101 72-73 0.13823683320588032 1101 74-75 0.08454064179566956 1101 76-77 0.08308544746711277 1101 78-79 0.08748115713866156 1101 80-81 0.03593649714787972 1101 82-83 0.054133304680206606 1101 84-85 0.14707262470108873 1101 86-87 0.13454153244343559 1101 88-89 0.1949719637034164 1101 90-91 0.15982760622845404 1101 92-93 0.17035726239758375 1101 94-95 0.07828855946203461 1101 96-97 0.10524480790529722 1101 98-99 0.06479424749640117 1101 100 0.10089722025833936 1102 1 0.031142226762622016 1102 2 0.1246342820125328 1102 3 0.1390413018785175 1102 4 0.07662845628647119 1102 5 0.11648320569767634 1102 6 0.19861509364493202 1102 7 0.20730250056183763 1102 8 0.2470441761332154 1102 9 0.24007168906687326 1102 10-11 0.24058644555982056 1102 12-13 0.267712721872833 1102 14-15 0.32233296420407953 1102 16-17 0.23647012601295359 1102 18-19 0.23729776776218614 1102 20-21 0.2955922840343277 1102 22-23 0.30609859549652896 1102 24-25 0.28548877224610436 1102 26-27 0.22999487200498692 1102 28-29 0.22877224551962883 1102 30-31 0.3197883667062058 1102 32-33 0.3451250377033901 1102 34-35 0.3457808711692536 1102 36-37 0.39548547488007557 1102 38-39 0.429330044304578 1102 40-41 0.3282068028150604 1102 42-43 0.3922261160635543 1102 44-45 0.3288685308086343 1102 46-47 0.3350991595138524 1102 48-49 0.3015657504276916 1102 50-51 0.3182130324029515 1102 52-53 0.3095452070578162 1102 54-55 0.3132530577043866 1102 56-57 0.21873806627550607 1102 58-59 0.27158308794343355 1102 60-61 0.22256815030627308 1102 62-63 0.2280326336586853 1102 64-65 0.22812050971166542 1102 66-67 0.2942253299251121 1102 68-69 0.2233013137378066 1102 70-71 0.20804000546739587 1102 72-73 0.2517987557049537 1102 74-75 0.2523083926452472 1102 76-77 0.252730174890047 1102 78-79 0.24394126067362976 1102 80-81 0.2671252939317341 1102 82-83 0.19461300499040846 1102 84-85 0.2756892957942796 1102 86-87 0.23615004633434467 1102 88-89 0.2144305596100864 1102 90-91 0.30054217331987587 1102 92-93 0.32723853824570526 1102 94-95 0.3575140026418957 1102 96-97 0.36014742764602303 1102 98-99 0.33768481317133237 1102 100 0.20919174265818086 1103 1 -0.0696712220377087 1103 2 -0.01914768815345269 1103 3 0.017820221869119024 1103 4 0.09220475395061101 1103 5 0.009797860141659953 1103 6 0.10634100241171751 1103 7 0.07741552192543111 1103 8 0.1125802856757332 1103 9 0.13853588454189492 1103 10-11 0.15209410809501378 1103 12-13 0.21684409169011332 1103 14-15 0.22227360943983143 1103 16-17 0.2568974906202115 1103 18-19 0.23129649616879533 1103 20-21 0.22785968755192698 1103 22-23 0.25551406441977775 1103 24-25 0.14925248257836898 1103 26-27 0.07023477448180415 1103 28-29 0.06917274591326361 1103 30-31 0.1359971120536585 1103 32-33 0.08077751761963015 1103 34-35 0.11936887697282828 1103 36-37 0.18075008789938352 1103 38-39 0.24035112142867376 1103 40-41 0.2396719586831466 1103 42-43 0.16940374550021886 1103 44-45 0.2712579825602148 1103 46-47 0.31158271948071103 1103 48-49 0.23194930998236885 1103 50-51 0.28437623121255484 1103 52-53 0.2353549977086189 1103 54-55 0.20672181813783652 1103 56-57 0.2520996686892687 1103 58-59 0.2168570163632353 1103 60-61 0.1518598295102649 1103 62-63 0.1583826000655364 1103 64-65 0.21204201509687692 1103 66-67 0.22163785378036494 1103 68-69 0.2752534259842676 1103 70-71 0.2256459063202101 1103 72-73 0.20851990990780678 1103 74-75 0.14243004930266778 1103 76-77 0.19032080425663622 1103 78-79 0.17953964119538313 1103 80-81 0.24140999688999187 1103 82-83 0.24341936625483385 1103 84-85 0.15889236122053774 1103 86-87 0.13780512714338045 1103 88-89 0.20517137095674087 1103 90-91 0.18116500676792668 1103 92-93 0.1985480321226909 1103 94-95 0.15009409035504717 1103 96-97 0.2205468254424794 1103 98-99 0.2104801340374145 1103 100 0.22977290411550655 1104 1 -0.033408253752181594 1104 2 0.04680896282987135 1104 3 0.10090042345582617 1104 4 0.10528918807073495 1104 5 0.05423601593825822 1104 6 0.1410010217041915 1104 7 0.2020715055465132 1104 8 0.21499479834380253 1104 9 0.21886543156239213 1104 10-11 0.20424851681993772 1104 12-13 0.2174925059665398 1104 14-15 0.24942678777713212 1104 16-17 0.23429551609415 1104 18-19 0.2915699867292645 1104 20-21 0.2569635224240585 1104 22-23 0.23439298443474144 1104 24-25 0.19601016511867186 1104 26-27 0.16209428390165925 1104 28-29 0.17722784759249777 1104 30-31 0.22109588757189158 1104 32-33 0.1446560439105511 1104 34-35 0.14405611243948613 1104 36-37 0.2106157787866323 1104 38-39 0.14670656564781837 1104 40-41 0.2359168156628897 1104 42-43 0.1537544453446955 1104 44-45 0.19937160147472355 1104 46-47 0.1338675000779901 1104 48-49 0.14629021594137726 1104 50-51 0.13820150632722772 1104 52-53 0.1487015043920792 1104 54-55 0.1461589404088386 1104 56-57 0.1314640996596026 1104 58-59 0.10488362226516301 1104 60-61 0.16374586608722552 1104 62-63 0.1734568170468549 1104 64-65 0.08806299074504409 1104 66-67 0.09567322988525717 1104 68-69 0.18163667696365593 1104 70-71 0.1833589602407102 1104 72-73 0.17382470152936946 1104 74-75 0.21136079324423918 1104 76-77 0.1340492724732627 1104 78-79 0.14521404125837734 1104 80-81 0.16206303742387718 1104 82-83 0.2890399245555173 1104 84-85 0.3225385903285556 1104 86-87 0.21225268310141132 1104 88-89 0.26148596630685716 1104 90-91 0.2604565987910732 1104 92-93 0.22712206208509045 1104 94-95 0.2412232416828779 1104 96-97 0.28789356836036717 1104 98-99 0.3162186619032319 1104 100 0.26413172451828615 1105 1 -0.13172640189243978 1105 2 -0.09690427255127432 1105 3 -0.09916964384191829 1105 4 -0.047807384076094195 1105 5 -0.04577937046158098 1105 6 0.011635012391870703 1105 7 0.04692499151293106 1105 8 0.04930552313791736 1105 9 0.0785798126385373 1105 10-11 0.0682947612364515 1105 12-13 0.0507479604452854 1105 14-15 0.059175137289855684 1105 16-17 0.03516970031906652 1105 18-19 0.11452554843938856 1105 20-21 0.084901910544815 1105 22-23 0.1062652252389995 1105 24-25 0.08657498802121921 1105 26-27 0.1177014430751413 1105 28-29 0.1240621550032941 1105 30-31 0.17804261038708802 1105 32-33 0.093777007051834 1105 34-35 0.09035923398494816 1105 36-37 0.13335711446816845 1105 38-39 0.15775305387958838 1105 40-41 0.1954538980700704 1105 42-43 0.13441516102533768 1105 44-45 0.14315869157510974 1105 46-47 0.08951655760181865 1105 48-49 0.07252355424570567 1105 50-51 0.004846868747954147 1105 52-53 0.14735791661503583 1105 54-55 0.13373880174187036 1105 56-57 0.12724609567807477 1105 58-59 0.043773813080193236 1105 60-61 0.10440833345030853 1105 62-63 0.09061146930238095 1105 64-65 0.07501934781367225 1105 66-67 0.014555893200054015 1105 68-69 0.14197692944264162 1105 70-71 0.05866615563142119 1105 72-73 0.21106239300127072 1105 74-75 0.15292703406270647 1105 76-77 0.12477979506641645 1105 78-79 0.10273266472002973 1105 80-81 0.10766122514268517 1105 82-83 0.0989819059924244 1105 84-85 0.10928853292544716 1105 86-87 0.011188891087662967 1105 88-89 -0.027904992644593563 1105 90-91 0.05227505821004286 1105 92-93 0.09058172959682409 1105 94-95 0.07798963589191388 1105 96-97 0.18367106681580836 1105 98-99 0.12765833182944775 1105 100 0.164893706381271 1106 1 -0.015042013111237651 1106 2 0.017613367612593578 1106 3 0.09403553941377751 1106 4 0.1360224619514696 1106 5 0.08231634430854484 1106 6 0.08821929041550192 1106 7 0.11446552774573604 1106 8 0.1355053464134457 1106 9 0.0807853015724902 1106 10-11 0.13610060994061257 1106 12-13 0.1160774540623919 1106 14-15 0.15220951096775082 1106 16-17 0.16786327660565803 1106 18-19 0.14583454219803826 1106 20-21 0.17740809748174513 1106 22-23 0.16173395372130273 1106 24-25 0.15145725222566142 1106 26-27 0.13640222469940966 1106 28-29 0.15786398428672754 1106 30-31 0.18690475129979234 1106 32-33 0.1975378880915244 1106 34-35 0.16330219315815953 1106 36-37 0.19113459424350765 1106 38-39 0.22873768553277785 1106 40-41 0.23631251532974318 1106 42-43 0.217620150127928 1106 44-45 0.11177628085280134 1106 46-47 0.20468546854289116 1106 48-49 0.19836266814900227 1106 50-51 0.17681269599594174 1106 52-53 0.20763893291170632 1106 54-55 0.16046235075221205 1106 56-57 0.20742477974447127 1106 58-59 0.2120441392167649 1106 60-61 0.28462092658170945 1106 62-63 0.22640029755228852 1106 64-65 0.20898367872822377 1106 66-67 0.23103624061671013 1106 68-69 0.15965068630966783 1106 70-71 0.264199428732141 1106 72-73 0.21957131072415592 1106 74-75 0.17657262428136278 1106 76-77 0.2051929077154 1106 78-79 0.24890516207526048 1106 80-81 0.26638666872608496 1106 82-83 0.20790193162063275 1106 84-85 0.14978728966832477 1106 86-87 0.20659030714105597 1106 88-89 0.2734777838079765 1106 90-91 0.16474226101018274 1106 92-93 0.18585216003425487 1106 94-95 0.12310359247476299 1106 96-97 0.07657915067369814 1106 98-99 0.14441026782936106 1106 100 0.11695405458356944 1107 1 -0.08672814722301325 1107 2 -0.062041249993097125 1107 3 -0.04804713935455851 1107 4 0.03605247208032125 1107 5 -0.01897897780649771 1107 6 0.1030508210115002 1107 7 0.059526012487019386 1107 8 0.06309088823095266 1107 9 0.12435248023014367 1107 10-11 0.06677393155767675 1107 12-13 0.045531592611112615 1107 14-15 0.11299803142005516 1107 16-17 0.07053842576704739 1107 18-19 0.11136623430999748 1107 20-21 0.16567319604723707 1107 22-23 0.10547925060919283 1107 24-25 0.07772354843357476 1107 26-27 0.04750282977129672 1107 28-29 0.09179214438955796 1107 30-31 0.07531369300589574 1107 32-33 -0.012079020008762598 1107 34-35 0.04586623922802602 1107 36-37 0.09713818268136265 1107 38-39 0.17306855117477937 1107 40-41 0.138060208193167 1107 42-43 0.08030304701360791 1107 44-45 0.014822585203859262 1107 46-47 0.05729748937800849 1107 48-49 0.09494941313439398 1107 50-51 0.12475785688857144 1107 52-53 0.07166248654608864 1107 54-55 0.07521126715863602 1107 56-57 0.12435987705637075 1107 58-59 0.05520249462026072 1107 60-61 0.12563195193699528 1107 62-63 0.054593604913684146 1107 64-65 0.10095593472670572 1107 66-67 0.00340867943869938 1107 68-69 0.08067259163168927 1107 70-71 0.0713761490221998 1107 72-73 0.0844911968030857 1107 74-75 0.1016982593202016 1107 76-77 0.11573893838374971 1107 78-79 0.11359362654778948 1107 80-81 0.08279146046995578 1107 82-83 0.08060291055863189 1107 84-85 0.09941434733921994 1107 86-87 0.06707433184121925 1107 88-89 0.09476388359286148 1107 90-91 -0.022493574744281375 1107 92-93 0.024110947493813484 1107 94-95 0.058547583524909186 1107 96-97 0.03654036370451408 1107 98-99 0.025550645818849915 1107 100 -0.06976696414461259 1108 1 0.07051147365965704 1108 2 0.15275925951642222 1108 3 0.11700441489346503 1108 4 0.2016791631061139 1108 5 0.20840710867976497 1108 6 0.18238059071948243 1108 7 0.12301040048567558 1108 8 0.18868937975656763 1108 9 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2214 58-59 -0.3222213387890349 2214 60-61 -0.2590682888298801 2214 62-63 -0.18008085177324773 2214 64-65 -0.13704757646890897 2214 66-67 -0.17333895150011358 2214 68-69 -0.17610090709693083 2214 70-71 -0.17754846794024104 2214 72-73 -0.09898349554153185 2214 74-75 -0.1472028683670601 2214 76-77 -0.14695136804340336 2214 78-79 -0.1676122183055142 2214 80-81 -0.20404776926078938 2214 82-83 -0.1454384978222265 2214 84-85 -0.20403371228066902 2214 86-87 -0.20846053086162186 2214 88-89 -0.24545012354902696 2214 90-91 -0.2015669325805476 2214 92-93 -0.2603655353758292 2214 94-95 -0.2903056419169978 2214 96-97 -0.19062795257557852 2214 98-99 -0.23773663458348793 2214 100 -0.20410339175997905 2215 1 -0.18193926518500447 2215 2 -0.1549566690410451 2215 3 -0.2216502726324734 2215 4 -0.13710881743605086 2215 5 -0.06951779572995065 2215 6 -0.13945459145032402 2215 7 -0.0980371201206367 2215 8 -0.11307725352030928 2215 9 -0.18293647209958408 2215 10-11 -0.1771739520369735 2215 12-13 -0.21564503810690638 2215 14-15 -0.2401015916743674 2215 16-17 -0.3094169624263827 2215 18-19 -0.27874481523472383 2215 20-21 -0.30968547628859255 2215 22-23 -0.29470932288369767 2215 24-25 -0.21304844910920195 2215 26-27 -0.305567327605452 2215 28-29 -0.3135572963014184 2215 30-31 -0.3054058157984585 2215 32-33 -0.31220336533731086 2215 34-35 -0.29251905648423815 2215 36-37 -0.26819273081837025 2215 38-39 -0.27063446165580274 2215 40-41 -0.21039721533583133 2215 42-43 -0.24598110446318344 2215 44-45 -0.15801841551154894 2215 46-47 -0.15678344577725767 2215 48-49 -0.14795911164563336 2215 50-51 -0.18621164758467756 2215 52-53 -0.14340004751698388 2215 54-55 -0.21566219185532987 2215 56-57 -0.24000221784680775 2215 58-59 -0.2669918152443529 2215 60-61 -0.2920574118051462 2215 62-63 -0.3365836776002098 2215 64-65 -0.42945332492121935 2215 66-67 -0.41678931740371183 2215 68-69 -0.40817800681348615 2215 70-71 -0.3815573016249232 2215 72-73 -0.47067388079754835 2215 74-75 -0.39883727122317225 2215 76-77 -0.35159533150972777 2215 78-79 -0.3712755854267371 2215 80-81 -0.44991758069145504 2215 82-83 -0.3747215588241417 2215 84-85 -0.39681237225875776 2215 86-87 -0.3430855115119229 2215 88-89 -0.35389054133431586 2215 90-91 -0.42028679839603456 2215 92-93 -0.4339378937437637 2215 94-95 -0.44472314663699564 2215 96-97 -0.3513107362968775 2215 98-99 -0.28952449815135495 2215 100 -0.2691943493980098 2216 1 -0.27099232479934443 2216 2 -0.3771502777395348 2216 3 -0.3264819497175644 2216 4 -0.4261615010231097 2216 5 -0.3277731613258936 2216 6 -0.4694618875190528 2216 7 -0.39818295033702356 2216 8 -0.2614602963307817 2216 9 -0.4040858644337959 2216 10-11 -0.3722872439290654 2216 12-13 -0.5129487857657082 2216 14-15 -0.4629509691611986 2216 16-17 -0.610774882398438 2216 18-19 -0.5714598905900203 2216 20-21 -0.5119805474027572 2216 22-23 -0.43062492481392667 2216 24-25 -0.5405771841454552 2216 26-27 -0.5844598110366164 2216 28-29 -0.47167221001056703 2216 30-31 -0.6275506947797709 2216 32-33 -0.6720162644176284 2216 34-35 -0.8225888471475713 2216 36-37 -0.8924700889965465 2216 38-39 -0.840212128324481 2216 40-41 -0.7310157597023306 2216 42-43 -0.7624446898103869 2216 44-45 -0.7223525241827531 2216 46-47 -0.724505645710046 2216 48-49 -0.7941219982315886 2216 50-51 -0.7105363111342697 2216 52-53 -0.7087496292550028 2216 54-55 -0.7145245384091439 2216 56-57 -0.6848934257478518 2216 58-59 -0.698670079693855 2216 60-61 -0.7145209283279641 2216 62-63 -0.622057008776892 2216 64-65 -0.6828408179575973 2216 66-67 -0.7172962477574245 2216 68-69 -0.7242206751987865 2216 70-71 -0.668990162238785 2216 72-73 -0.7292474737234045 2216 74-75 -0.7080155900826881 2216 76-77 -0.5760558149736994 2216 78-79 -0.5815250009037243 2216 80-81 -0.5176394387812664 2216 82-83 -0.6884886354479036 2216 84-85 -0.5582020847676716 2216 86-87 -0.6047410183345932 2216 88-89 -0.7141539324919037 2216 90-91 -0.6588587311044733 2216 92-93 -0.7806541329604677 2216 94-95 -0.6761327851733654 2216 96-97 -0.638793790855356 2216 98-99 -0.5807699620374116 2216 100 -0.5482612866979686 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 25232.0 3 4815.0 4 2736.0 5 2372.0 6 1981.0 7 2097.0 8 2418.0 9 2815.0 10 3168.0 11 3346.0 12 3901.0 13 4328.0 14 4815.0 15 4849.0 16 4896.0 17 4963.0 18 4849.0 19 4759.0 20 4964.0 21 4931.0 22 5856.0 23 8036.0 24 12285.0 25 16779.0 26 17868.0 27 15281.0 28 16437.0 29 20959.0 30 25165.0 31 30128.0 32 39487.0 33 53658.0 34 77170.0 35 118779.0 36 215012.0 37 450092.0 38 734022.0 39 317156.0 40 686.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.84460976361315 19.99683056741647 13.348637584188053 23.809922084782322 2 15.29659987536045 20.394982377939506 40.70687172337062 23.601546023329426 3 17.8376634323894 25.67682786992404 30.161752011941132 26.323756685745426 4 11.91115525552616 16.908793206456295 39.778007011741565 31.40204452627598 5 12.455197132616487 38.275562952982305 36.06395251556542 13.205287398835786 6 30.45798578897102 38.751759646296236 18.06060888935661 12.729645675376137 7 26.843777837369654 32.619287601117215 23.063641923854792 17.47329263765834 8 24.59261059945683 36.991393550733925 20.717220252966104 17.69877559684314 9 24.64507736540595 17.07763667037013 21.463753392456645 36.813532571767276 10-11 23.33119233309061 27.216876572033282 29.445395796226393 20.006535298649716 12-13 24.087720148152716 24.607707992935882 29.254766298340108 22.049805560571293 14-15 21.504451046416484 26.70625647927381 26.179613176894197 25.60967929741551 16-17 21.149598457668883 29.949211201888552 26.723664647764984 22.177525692677584 18-19 20.970365656836993 29.042669138580575 28.635573982372204 21.351391222210232 20-21 22.383138443897888 28.335304207837247 28.03743832256306 21.244119025701806 22-23 21.953757561298126 28.13445574940377 28.08924862859995 21.82253806069815 24-25 21.795852830211928 28.20755738176685 28.09508639057004 21.901503397451187 26-27 21.556318577799402 28.045783097485423 28.22563834067783 22.17225998403735 28-29 21.527857414061597 28.143774521402747 28.308921526008508 22.01944653852715 30-31 21.817691929068857 28.711654470002017 27.824905146020978 21.645748454908148 32-33 21.41872712764494 28.351473461189226 28.43382219409515 21.79597721707069 34-35 21.56617066781307 27.975954619768068 28.529280858498314 21.928593853920553 36-37 21.957863216882885 28.503345532317404 27.820726212586276 21.71806503821343 38-39 21.772308556590236 28.606168965679686 28.047467196633523 21.574055281096552 40-41 21.47501941701406 28.255387891180185 28.33521339342481 21.934379298380946 42-43 21.447946389379666 28.09089432190059 28.377178944068724 22.083980344651025 44-45 21.614556161489958 28.357663896156726 28.279166848270886 21.74861309408243 46-47 21.619635569998927 28.59712875600634 28.140264889902916 21.642970784091812 48-49 21.531241091388186 28.34906018616866 28.20751300726117 21.91218571518198 50-51 21.54248440251215 28.21591770328904 28.21734851401501 22.0242493801838 52-53 21.621298560413077 28.22617073752744 28.20864808796194 21.943882614097543 54-55 21.752353507385795 28.65612191464311 27.998401096340768 21.59312348163033 56-57 21.535556987305533 28.352501638233562 28.075174064379883 22.036767310081018 58-59 21.360604022236586 27.92812642522447 28.54937021586813 22.161899336670817 60-61 21.761760100508752 28.399290733623197 28.00468647582389 21.834262690044167 62-63 21.601946555996722 28.39701888488615 28.027042935083834 21.973991624033292 64-65 21.550135544303487 28.078889514003897 28.278464083621017 22.0925108580716 66-67 21.367233526175184 28.22633703278884 28.09803259931928 22.308396841716704 68-69 21.962168779822527 28.298246625781452 27.993962747236157 21.745621847159864 70-71 21.818521966690657 28.110210918086835 28.097335296590494 21.973931818632014 72-73 21.52254586215526 28.239480002579477 28.251342944443795 21.986631190821473 74-75 21.632522890478516 28.271108310518606 28.109470201605248 21.986898597397637 76-77 21.605186614597454 28.27746398526662 28.00169729665013 22.115652103485797 78-79 21.743910898584815 28.392295002306412 28.063543386938317 21.800250712170456 80-81 21.64652295054441 28.09689141759078 28.181000537620566 22.075585094244246 82-83 21.45575900853663 28.18085803845171 28.3770361956843 21.986346757327354 84-85 21.491245346611304 28.11715151545826 28.192521173372437 22.199081964557994 86-87 21.71089389009221 28.29686295557165 28.050734391741173 21.94150876259497 88-89 21.468036881367865 28.228263979050677 28.12250511629954 22.181194023281915 90-91 21.35154166409943 28.05610812333313 28.28458511939865 22.307765093168797 92-93 21.52409597326941 28.242502102080692 28.12406820225834 22.109333722391554 94-95 21.306374889231243 28.00920786281596 28.311185449622688 22.373231798330114 96-97 21.33919452740052 27.993311971487415 28.33755002762839 22.32994347348368 98-99 21.374121058226862 28.058534721844758 28.251746220437596 22.315597999490784 100 21.477774100065847 28.029167972450026 28.097039363646285 22.396018563837842 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 362.0 1 338.0 2 328.0 3 329.0 4 304.0 5 289.5 6 281.5 7 246.5 8 223.5 9 215.0 10 194.5 11 189.5 12 187.5 13 220.0 14 265.5 15 287.5 16 370.5 17 519.5 18 748.5 19 1099.0 20 1596.5 21 2343.5 22 3514.5 23 5414.0 24 7748.0 25 10448.0 26 14160.0 27 18898.5 28 24726.0 29 31484.5 30 38497.0 31 45363.0 32 52157.5 33 59195.5 34 66581.0 35 72288.0 36 76448.5 37 80525.5 38 83327.0 39 84483.5 40 85185.5 41 85448.0 42 83930.0 43 82110.5 44 80116.0 45 77696.0 46 76106.5 47 74292.5 48 72115.5 49 69868.5 50 67589.0 51 65030.0 52 60817.5 53 57636.0 54 55644.5 55 53090.0 56 49223.5 57 44309.5 58 40405.0 59 36848.0 60 32771.5 61 27952.5 62 23601.0 63 19503.5 64 15585.5 65 12598.5 66 9854.5 67 7243.5 68 5428.0 69 4187.0 70 3042.0 71 2180.0 72 1591.5 73 1166.0 74 843.0 75 597.0 76 425.5 77 315.0 78 213.5 79 139.5 80 97.0 81 62.0 82 40.0 83 31.5 84 25.5 85 18.5 86 20.5 87 17.5 88 9.5 89 7.5 90 7.0 91 5.0 92 4.0 93 2.5 94 2.0 95 2.5 96 1.5 97 1.0 98 1.0 99 1.5 100 8.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.06119420647919507 2 0.04130938884540918 3 0.05631098798948216 4 0.0773396225668044 5 0.08934970047393614 6 0.08965765118950363 7 0.09185729915784277 8 0.10347144043067347 9 0.09872020081906091 10-11 0.1031854861947894 12-13 0.10826667300165281 14-15 0.10509917992724445 16-17 0.07584386194833379 18-19 0.09880818673779448 20-21 0.09249519706866113 22-23 0.0582906711609874 24-25 0.022216444480225386 26-27 0.1779075276792702 28-29 0.06504359042378857 30-31 0.07500799572036491 32-33 0.0727423583129756 34-35 0.02074268034143816 36-37 0.051933688532487254 38-39 0.07716365072933729 40-41 0.08301471432511941 42-43 0.07698767889187015 44-45 0.07338025622379395 46-47 0.08118900651139792 48-49 0.07758158384332171 50-51 0.07265437239424202 52-53 0.07676771409503623 54-55 0.06750719614832842 56-57 0.06992680891350149 58-59 0.06682530527814329 60-61 0.0637018051631017 62-63 0.07003679131191845 64-65 0.06638537568447546 66-67 0.06777115390452912 68-69 0.06722124191244433 70-71 0.05965445290135767 72-73 0.05765277325016904 74-75 0.05936849866547358 76-77 0.054683248492911195 78-79 0.05116381174356856 80-81 0.04586266013987121 82-83 0.05175771669502013 84-85 0.042079265634327886 86-87 0.03979163174725517 88-89 0.04139737476414275 90-91 0.03831786760846794 92-93 0.041243399406359 94-95 0.0397036458285216 96-97 0.04273916002482963 98-99 0.04265117410609606 100 0.050459924393700034 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2273091.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 72.0819643322129 #Duplication Level Percentage of deduplicated Percentage of total 1 81.02484345274279 58.404298757837395 2 12.57340723196904 18.126317832583602 3 3.3147882513205134 7.168093455015708 4 1.2241847191474402 3.5296655704650335 5 0.6043634189805887 2.1781851205326515 6 0.3474564176399012 1.5027204661990696 7 0.20694467455520424 1.0441885055020748 8 0.13904440816166383 0.8018075255762165 9 0.09665510148557695 0.6270380620028797 >10 0.4488131608932409 5.48987971621974 >50 0.016438048054123935 0.7960338031860853 >100 0.003000087764600483 0.30766484790112814 >500 6.102728529428216E-5 0.024106336978270762 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 8.798591873356587E-5 0.0 10-11 0.0 0.0 0.0 8.798591873356587E-5 0.0 12-13 0.0 0.0 0.0 8.798591873356587E-5 0.0 14-15 0.0 0.0 0.0 1.3197887810034882E-4 0.0 16-17 0.0 0.0 0.0 1.979683171505232E-4 0.0 18-19 0.0 0.0 0.0 2.199647968339147E-4 0.0 20-21 0.0 0.0 0.0 2.419612765173062E-4 0.0 22-23 0.0 0.0 0.0 2.6395775620069765E-4 0.0 24-25 0.0 0.0 0.0 3.0795071556748057E-4 0.0 26-27 0.0 0.0 0.0 4.399295936678294E-4 0.0 28-29 0.0 0.0 0.0 7.918732686020928E-4 0.0 30-31 0.0 0.0 0.0 0.0027495599604239335 0.0 32-33 0.0 0.0 0.0 0.008292672840638584 0.0 34-35 0.0 0.0 0.0 0.01665133512032734 0.0 36-37 0.0 0.0 0.0 0.030795071556748058 0.0 38-39 0.0 0.0 0.0 0.05578307247708077 0.0 40-41 0.0 0.0 0.0 0.08655614755414544 0.0 42-43 0.0 0.0 0.0 0.11759318038741079 6.598943905017441E-5 44-45 0.0 0.0 0.0 0.15305150563703784 1.3197887810034882E-4 46-47 0.0 0.0 0.0 0.1978143417927395 1.3197887810034882E-4 48-49 0.0 0.0 0.0 0.24792232251150526 1.7597183746713175E-4 50-51 0.0 0.0 0.0 0.3064329584693266 1.7597183746713175E-4 52-53 0.0 0.0 0.0 0.3654275169801825 1.7597183746713175E-4 54-55 0.0 0.0 0.0 0.42822746647626514 1.7597183746713175E-4 56-57 0.0 0.0 0.0 0.5051051629697183 1.7597183746713175E-4 58-59 0.0 0.0 0.0 0.5931790676220178 1.7597183746713175E-4 60-61 0.0 0.0 0.0 0.6880278880168018 1.7597183746713175E-4 62-63 0.0 0.0 0.0 0.7866381064374457 1.7597183746713175E-4 64-65 0.0 0.0 0.0 0.892463170194242 1.7597183746713175E-4 66-67 0.0 0.0 0.0 1.0137077662090959 1.7597183746713175E-4 68-69 0.0 0.0 0.0 1.1501739261648565 2.199647968339147E-4 70-71 0.0 0.0 0.0 1.3001459246462197 2.199647968339147E-4 72-73 0.0 0.0 0.0 1.464635599718621 2.419612765173062E-4 74-75 0.0 0.0 0.0 1.6323587573044809 2.6395775620069765E-4 76-77 0.0 0.0 0.0 1.8080886334950954 2.6395775620069765E-4 78-79 0.0 0.0 0.0 2.011622060005517 2.6395775620069765E-4 80-81 0.0 0.0 0.0 2.2404294416721546 2.6395775620069765E-4 82-83 0.0 0.0 0.0 2.479707147668087 2.6395775620069765E-4 84-85 0.0 2.199647968339147E-5 0.0 2.71542142395531 3.0795071556748057E-4 86-87 0.0 4.399295936678294E-5 0.0 2.9675670705660266 3.0795071556748057E-4 88 0.0 4.399295936678294E-5 0.0 3.1776554480220986 3.0795071556748057E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 2850 0.0 35.966133 1 TCAACGC 2815 0.0 35.24175 4 ATCAACG 2885 0.0 34.549637 3 CAACGCA 2935 0.0 34.12125 5 AACGCAG 3075 0.0 32.55414 6 GGTATCA 1790 0.0 31.521751 1 TATCAAC 3520 0.0 28.183388 2 GTACATG 6055 0.0 27.17916 1 ACGCAGA 3815 0.0 25.746246 7 TACATGG 6110 0.0 25.470661 2 CGCAGAG 3835 0.0 25.024591 8 ACATGGG 6095 0.0 24.221964 3 TACACCG 180 2.1553751E-7 23.508419 5 CATGGGG 4245 0.0 21.376326 4 GTATAGA 1085 0.0 19.50136 1 GCAGAGT 4715 0.0 19.460787 9 GAGTACA 4430 0.0 19.10517 1 GTCTAGG 715 0.0 17.755783 1 ACCGGTC 190 2.0096135E-4 17.331919 8 ACCGTCC 225 3.8454255E-5 16.726677 8 >>END_MODULE