FastQCFastQC Report
Wed 25 May 2016
SRR1033087_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033087_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2273091
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39730.17478402756422862No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33510.14742040683808963No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA32350.053.489041
GTATCAA59850.042.3041731
TCAACGC70950.035.0419964
ATCAACG72100.034.6801573
CAACGCA72300.034.3219225
TATCAAC75100.033.3209082
AACGCAG75500.032.991726
ACGCAGA87200.028.4572857
CGCAGAG88050.028.0758158
GTACATG60950.025.2957231
GCAGAGT97700.024.9659929
TACATGG62300.023.6765582
ACATGGG63700.023.020773
GTGGTAT16650.022.9373821
GAGTACT68450.021.28407112-13
AGAGTAC91400.021.1592210-11
CAGAGTA96000.021.14893510-11
TGGTATC15800.020.580422
GTCGTAC1150.003887850620.4995921
CATGGGG45250.019.5265184
Produced by FastQC (version 0.11.5)