##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1033087_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2273091 Sequences flagged as poor quality 0 Sequence length 100 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.705650147750355 34.0 31.0 34.0 31.0 34.0 2 32.700056003037275 34.0 31.0 34.0 31.0 34.0 3 32.80032519595564 34.0 31.0 34.0 31.0 34.0 4 36.29813104710722 37.0 37.0 37.0 35.0 37.0 5 36.28033369539539 37.0 37.0 37.0 35.0 37.0 6 36.35016459965747 37.0 37.0 37.0 35.0 37.0 7 36.299660682304406 37.0 37.0 37.0 35.0 37.0 8 36.30453246262468 37.0 37.0 37.0 35.0 37.0 9 38.10318152682844 39.0 39.0 39.0 37.0 39.0 10-11 38.06608622356079 39.0 39.0 39.0 35.0 39.0 12-13 38.03985608143273 39.0 39.0 39.0 35.0 39.0 14-15 39.53315177439003 41.0 39.0 41.0 37.0 41.0 16-17 39.538707645228456 41.0 39.0 41.0 37.0 41.0 18-19 39.55836325954394 41.0 39.5 41.0 37.0 41.0 20-21 39.55416259181881 41.0 40.0 41.0 37.0 41.0 22-23 39.48239687720377 41.0 39.0 41.0 36.5 41.0 24-25 39.43031493239822 41.0 39.0 41.0 36.0 41.0 26-27 39.326699415025615 41.0 39.0 41.0 36.0 41.0 28-29 39.273222453478546 41.0 39.0 41.0 36.0 41.0 30-31 39.26641388312214 41.0 39.0 41.0 36.0 41.0 32-33 39.21563984899856 41.0 39.0 41.0 35.0 41.0 34-35 39.134088560466786 41.0 39.0 41.0 35.0 41.0 36-37 39.04329831053838 41.0 39.0 41.0 35.0 41.0 38-39 38.94477255859972 41.0 39.0 41.0 35.0 41.0 40-41 38.84038628457901 41.0 39.0 41.0 35.0 41.0 42-43 38.720635469499456 41.0 39.0 41.0 35.0 41.0 44-45 38.55535172151049 41.0 38.0 41.0 35.0 41.0 46-47 38.35552206224916 41.0 38.0 41.0 34.5 41.0 48-49 38.144658308884246 40.0 38.0 41.0 34.0 41.0 50-51 38.00828519403755 40.0 38.0 41.0 34.0 41.0 52-53 37.873812575035494 40.0 38.0 41.0 33.5 41.0 54-55 37.7302276943598 40.0 37.5 41.0 33.0 41.0 56-57 37.58642284888727 40.0 37.0 41.0 33.0 41.0 58-59 37.41248370610768 40.0 37.0 41.0 33.0 41.0 60-61 37.22658705700739 40.0 36.0 41.0 33.0 41.0 62-63 36.998144816903505 39.5 36.0 41.0 32.5 41.0 64-65 36.73499895076792 39.0 35.0 41.0 32.0 41.0 66-67 36.463234643927585 39.0 35.0 41.0 32.0 41.0 68-69 36.1613630954502 38.0 35.0 40.5 31.0 41.0 70-71 35.8349683316682 37.0 35.0 40.0 31.0 41.0 72-73 35.49389047776794 37.0 35.0 39.5 31.0 41.0 74-75 35.149706281006786 36.0 35.0 39.0 31.0 41.0 76-77 34.79410855086752 36.0 35.0 39.0 30.5 40.5 78-79 34.431844567595405 35.5 34.5 37.5 30.0 39.5 80-81 34.09378001144697 35.0 34.0 37.0 30.0 39.0 82-83 33.788478991822146 35.0 34.0 37.0 30.0 39.0 84-85 33.55760790043162 35.0 34.0 36.0 30.0 38.0 86-87 33.338500306410964 35.0 34.0 36.0 30.0 37.0 88-89 33.1250418483026 35.0 34.0 36.0 30.0 37.0 90-91 32.93872924577151 35.0 34.0 35.0 29.5 36.5 92-93 32.760621770091916 35.0 34.0 35.0 29.0 36.0 94-95 32.59885041997879 35.0 34.0 35.0 29.0 36.0 96-97 32.44959902617185 35.0 34.0 35.0 29.0 36.0 98-99 32.3204827699375 35.0 34.0 35.0 29.0 36.0 100 32.2136020951207 35.0 34.0 35.0 29.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.11183237096319232 1101 2 0.13301347650983786 1101 3 0.11589380432612018 1101 4 0.1316495200671426 1101 5 0.09684113916337367 1101 6 0.12859702945160478 1101 7 0.18162256427481793 1101 8 0.16971669121615207 1101 9 0.2205605401530093 1101 10-11 0.2094502198194661 1101 12-13 0.22087329384255128 1101 14-15 0.29505224873725666 1101 16-17 0.3199761928220184 1101 18-19 0.27331303688540487 1101 20-21 0.2481763672192585 1101 22-23 0.26562756756526795 1101 24-25 0.30597627405716565 1101 26-27 0.28666538543659925 1101 28-29 0.34039368944775106 1101 30-31 0.29358856230848573 1101 32-33 0.2821312872209276 1101 34-35 0.23279233213332162 1101 36-37 0.22058553616852805 1101 38-39 0.19351493376347406 1101 40-41 0.20680980654163506 1101 42-43 0.19774144761992574 1101 44-45 0.21447529160764844 1101 46-47 0.21518978854775384 1101 48-49 0.27337210409598356 1101 50-51 0.27239318798876866 1101 52-53 0.18848983479112746 1101 54-55 0.18248106229949457 1101 56-57 0.20462551640049043 1101 58-59 0.1595121477617596 1101 60-61 0.1837733474437897 1101 62-63 0.22214212641985398 1101 64-65 0.1926918942268614 1101 66-67 0.21311082137035697 1101 68-69 0.2146603861526657 1101 70-71 0.21830947028987424 1101 72-73 0.20806514553932942 1101 74-75 0.23448604615159496 1101 76-77 0.24604712119172234 1101 78-79 0.295182271231738 1101 80-81 0.2786339790927883 1101 82-83 0.2652948436036553 1101 84-85 0.31192245910768435 1101 86-87 0.34929687082730965 1101 88-89 0.42887702369211667 1101 90-91 0.3558769307748548 1101 92-93 0.31071194642363764 1101 94-95 0.343705015926588 1101 96-97 0.37827131385658674 1101 98-99 0.4407759652022776 1101 100 0.39779833455217783 1102 1 0.1551776091438839 1102 2 0.11030573605334837 1102 3 0.12664438396146238 1102 4 0.11753891526913662 1102 5 0.11133098937285979 1102 6 0.14647768384624982 1102 7 0.14625855502327312 1102 8 0.1733367610529939 1102 9 0.20422764642677294 1102 10-11 0.2212884518028133 1102 12-13 0.205906423164258 1102 14-15 0.37927942446067675 1102 16-17 0.3084954542907994 1102 18-19 0.28454906587381146 1102 20-21 0.32417689753202694 1102 22-23 0.30882277160626614 1102 24-25 0.35386941190129306 1102 26-27 0.36094359560708256 1102 28-29 0.32005587934366275 1102 30-31 0.33503632269412265 1102 32-33 0.3065843505167365 1102 34-35 0.2845031563650977 1102 36-37 0.20707463289014072 1102 38-39 0.20993331269000493 1102 40-41 0.2598182982890478 1102 42-43 0.19666749900631686 1102 44-45 0.21936083606028234 1102 46-47 0.1776464504877211 1102 48-49 0.19446545763253198 1102 50-51 0.13242454996377262 1102 52-53 0.24888159410483013 1102 54-55 0.20751637116752875 1102 56-57 0.16365483086357102 1102 58-59 0.22354763216810625 1102 60-61 0.22466387701047097 1102 62-63 0.19493270921357464 1102 64-65 0.13820997913214939 1102 66-67 0.14249966526894298 1102 68-69 0.1824292028482546 1102 70-71 0.1306750132720751 1102 72-73 0.13670865855037562 1102 74-75 0.15922040578588081 1102 76-77 0.14702089115648675 1102 78-79 0.19638421925021277 1102 80-81 0.19158492168132568 1102 82-83 0.15971273615636505 1102 84-85 0.11625591707974792 1102 86-87 0.14334072756281557 1102 88-89 0.14336934274898283 1102 90-91 0.11308732464410554 1102 92-93 0.20275267609746095 1102 94-95 0.2124104067364243 1102 96-97 0.2413912365414177 1102 98-99 0.20931874923991245 1102 100 0.22974073884410018 1103 1 0.0387672533862613 1103 2 0.053356694591570886 1103 3 0.07374558383450847 1103 4 0.09335267320886942 1103 5 0.06463960529394797 1103 6 0.06052332930870108 1103 7 0.08155238171560342 1103 8 0.13722639416635474 1103 9 0.16627285085035481 1103 10-11 0.20571014473258487 1103 12-13 0.13611083643828437 1103 14-15 0.21216072729775703 1103 16-17 0.16658058303705303 1103 18-19 0.24820194973219856 1103 20-21 0.23482360684619152 1103 22-23 0.18078583769202083 1103 24-25 0.09916528921829837 1103 26-27 0.08319050318264232 1103 28-29 0.14966571451054023 1103 30-31 0.2260087824956969 1103 32-33 0.11830722559556506 1103 34-35 0.21593985079935152 1103 36-37 0.1897098607670955 1103 38-39 0.27175083594759286 1103 40-41 0.20937633199761052 1103 42-43 0.1415398038260065 1103 44-45 0.13004155070812828 1103 46-47 0.1894106987225399 1103 48-49 0.19217859894221334 1103 50-51 0.23186815057216137 1103 52-53 0.22936758184641803 1103 54-55 0.20601652985908459 1103 56-57 0.21090209910176583 1103 58-59 0.14271695399870765 1103 60-61 0.15709826732575038 1103 62-63 0.09120969193062933 1103 64-65 0.18801238753414395 1103 66-67 0.11535856580437098 1103 68-69 0.13522176206905812 1103 70-71 0.06959452986049541 1103 72-73 0.1438779103093708 1103 74-75 0.2233777536171644 1103 76-77 0.22334168923075026 1103 78-79 0.190569564806232 1103 80-81 0.09233860353154455 1103 82-83 0.17201323819875114 1103 84-85 0.15786170045958414 1103 86-87 0.10756763175845663 1103 88-89 0.17986412447596223 1103 90-91 0.10700407958724867 1103 92-93 0.17727809559962537 1103 94-95 0.17837477739044516 1103 96-97 0.17309069946323774 1103 98-99 0.1611949677643736 1103 100 0.09990909615977728 1104 1 0.08096659586711752 1104 2 0.04353623275327578 1104 3 0.05225268071030342 1104 4 0.032880598086322266 1104 5 0.07458802744486093 1104 6 0.0958384069707634 1104 7 0.12165514368973618 1104 8 0.09204065485103996 1104 9 0.14810396292390493 1104 10-11 0.12780372309351407 1104 12-13 0.1521213783994213 1104 14-15 0.1908641940379141 1104 16-17 0.24988520732588881 1104 18-19 0.2220387412750071 1104 20-21 0.23387399368005646 1104 22-23 0.17194008892733592 1104 24-25 0.23378076847887996 1104 26-27 0.20851286473942565 1104 28-29 0.2049708998561286 1104 30-31 0.18294939906754593 1104 32-33 0.1700312447305521 1104 34-35 0.2024543103146712 1104 36-37 0.26086859061334167 1104 38-39 0.21560701839930374 1104 40-41 0.25149840613584473 1104 42-43 0.2727616279494782 1104 44-45 0.31780091641869745 1104 46-47 0.30422727253595383 1104 48-49 0.2687237197170589 1104 50-51 0.2488147714356046 1104 52-53 0.2701175277093597 1104 54-55 0.23742537290099364 1104 56-57 0.3129895747400937 1104 58-59 0.2668551905087355 1104 60-61 0.23097713812520482 1104 62-63 0.2522898246156302 1104 64-65 0.2489202659521581 1104 66-67 0.2295065469843678 1104 68-69 0.34367206425225305 1104 70-71 0.26811166669929065 1104 72-73 0.27545014882740304 1104 74-75 0.28130144989844297 1104 76-77 0.19986663164861085 1104 78-79 0.20139968272657427 1104 80-81 0.2587942997340704 1104 82-83 0.1923065133921682 1104 84-85 0.284871415600243 1104 86-87 0.3198808589175428 1104 88-89 0.28212582300146494 1104 90-91 0.2780230373997128 1104 92-93 0.29614756623765004 1104 94-95 0.2968650425635815 1104 96-97 0.36281302558485606 1104 98-99 0.3390049270139315 1104 100 0.36932313635900726 1105 1 0.012525995655494171 1105 2 0.07200012560711855 1105 3 0.10065876804350893 1105 4 0.06657965430822799 1105 5 0.03222240397610676 1105 6 0.06894866117776388 1105 7 0.10433526517127945 1105 8 0.07742830927723787 1105 9 0.08946852354750234 1105 10-11 0.09420527025519476 1105 12-13 0.11615365669916144 1105 14-15 0.1686237448497252 1105 16-17 0.16340109437550865 1105 18-19 0.17748469832091018 1105 20-21 0.18036601287281684 1105 22-23 0.10259358483573067 1105 24-25 0.10957677795690302 1105 26-27 0.17230866590927008 1105 28-29 0.13482288268964027 1105 30-31 0.07920103535198564 1105 32-33 0.09078039670320948 1105 34-35 0.11790746346142811 1105 36-37 0.09778742853774247 1105 38-39 0.13801458756118024 1105 40-41 0.1769856224047146 1105 42-43 0.10416479660099043 1105 44-45 0.0727469338266431 1105 46-47 0.04160060104032226 1105 48-49 0.035289546286293216 1105 50-51 0.05248762343499891 1105 52-53 0.13053755275368673 1105 54-55 0.08588330056597471 1105 56-57 0.044326226801508994 1105 58-59 0.07627474449251537 1105 60-61 0.08392860468582342 1105 62-63 0.05233645724284486 1105 64-65 0.09033619347984967 1105 66-67 0.07077973606428145 1105 68-69 0.05948336544458499 1105 70-71 0.07133286868484134 1105 72-73 0.1469668454383708 1105 74-75 0.12351936392870044 1105 76-77 0.13950862563135047 1105 78-79 0.07752046359820497 1105 80-81 0.03961621754228872 1105 82-83 0.11639048999536783 1105 84-85 0.08546391035111611 1105 86-87 0.05755173672348235 1105 88-89 0.04962430211502067 1105 90-91 0.10734028366022841 1105 92-93 0.1461900563726033 1105 94-95 0.09750180280323661 1105 96-97 0.07283975057781333 1105 98-99 0.08758144831462289 1105 100 0.07605835535481731 1106 1 0.046364443116992504 1106 2 0.035164857289792906 1106 3 0.048719110827583734 1106 4 0.06962009188416829 1106 5 0.05906619245397593 1106 6 0.08988884080098813 1106 7 0.07126846341017767 1106 8 0.05536547320611618 1106 9 0.008169576083069785 1106 10-11 0.10432674236916739 1106 12-13 0.12400755603845681 1106 14-15 0.19097293417021888 1106 16-17 0.1189547987038182 1106 18-19 0.12927201307966385 1106 20-21 0.14483852372845973 1106 22-23 0.1238394427147611 1106 24-25 0.12369229544039229 1106 26-27 0.12059404568402243 1106 28-29 0.1691991793953136 1106 30-31 0.12751910509088304 1106 32-33 0.18949605135811964 1106 34-35 0.17072198358494717 1106 36-37 0.18066529223955285 1106 38-39 0.2050886596786725 1106 40-41 0.21158307303903 1106 42-43 0.18044000997750942 1106 44-45 0.23347933350931527 1106 46-47 0.21072104685196535 1106 48-49 0.2205706641747014 1106 50-51 0.21880752363127698 1106 52-53 0.2537970040589599 1106 54-55 0.26391496738842335 1106 56-57 0.19352430676278942 1106 58-59 0.26784321709909165 1106 60-61 0.24488360315743307 1106 62-63 0.2814074978628298 1106 64-65 0.26899064024058816 1106 66-67 0.2319681246251406 1106 68-69 0.1621433080926593 1106 70-71 0.21880343755794485 1106 72-73 0.28886052449064437 1106 74-75 0.2930274838177951 1106 76-77 0.23155259273007545 1106 78-79 0.1994030533477371 1106 80-81 0.1312653765658709 1106 82-83 0.15528093662426556 1106 84-85 0.117590108061286 1106 86-87 0.07800188294785926 1106 88-89 0.15474213272953818 1106 90-91 0.14326402574002373 1106 92-93 0.13222746832079224 1106 94-95 0.11750882843640653 1106 96-97 0.16240320861614066 1106 98-99 0.20287967425194608 1106 100 0.2082074450702578 1107 1 -0.002389670325442239 1107 2 -0.006200341113768104 1107 3 0.02677493795543029 1107 4 0.024814779573347323 1107 5 0.046757463857780124 1107 6 0.07806212184049599 1107 7 0.06008865439063271 1107 8 -0.006202084635901883 1107 9 0.009298668330202986 1107 10-11 0.04472758479388972 1107 12-13 0.021637317038333492 1107 14-15 0.09251173892446474 1107 16-17 0.031214540685773784 1107 18-19 0.012051767742548236 1107 20-21 0.04504357855306296 1107 22-23 0.07924500422360836 1107 24-25 0.09753125052154843 1107 26-27 0.07958718168723067 1107 28-29 0.12363916759846205 1107 30-31 0.06922598383373213 1107 32-33 0.0726425581135075 1107 34-35 0.08768945661122984 1107 36-37 0.005515699172065069 1107 38-39 0.03491542974962414 1107 40-41 0.029524502522654927 1107 42-43 0.02308480021568471 1107 44-45 0.09337045096011565 1107 46-47 0.09950615119155515 1107 48-49 0.04988866068607223 1107 50-51 0.019305522553125343 1107 52-53 0.01135539517071038 1107 54-55 0.06702469360556762 1107 56-57 0.07505068960293926 1107 58-59 0.0719730804925689 1107 60-61 0.05310407331151623 1107 62-63 0.05911817397480945 1107 64-65 0.03147199614586782 1107 66-67 0.09521923688201639 1107 68-69 0.06246163474997957 1107 70-71 0.0898834292283226 1107 72-73 0.05622741886362803 1107 74-75 0.03658821323313788 1107 76-77 0.14114591404323562 1107 78-79 0.11589762510595847 1107 80-81 0.10370116818576491 1107 82-83 0.05740341075735955 1107 84-85 0.08435022111209634 1107 86-87 0.059964811287194664 1107 88-89 0.09910105732399188 1107 90-91 0.04938667047155576 1107 92-93 0.08482458769375967 1107 94-95 0.0626569866122324 1107 96-97 0.0714037953504203 1107 98-99 0.121251001421399 1107 100 0.10673444503468943 1108 1 -0.005903243820782222 1108 2 -0.03249411058510532 1108 3 0.03230169907053693 1108 4 -0.03402406080116549 1108 5 -0.009591651770200826 1108 6 0.005172747640777686 1108 7 0.039459685545779166 1108 8 0.049251528907610975 1108 9 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2214 52-53 -0.3248863265736759 2214 54-55 -0.29287199110875406 2214 56-57 -0.2853113786309933 2214 58-59 -0.28094840803037613 2214 60-61 -0.3320370153265486 2214 62-63 -0.3558844946377775 2214 64-65 -0.27693435720153303 2214 66-67 -0.2866585744342274 2214 68-69 -0.20718330122208073 2214 70-71 -0.19475614353965653 2214 72-73 -0.25361484722577643 2214 74-75 -0.347879155364744 2214 76-77 -0.25895809545696125 2214 78-79 -0.1785676088905177 2214 80-81 -0.179762605795311 2214 82-83 -0.20835313008569045 2214 84-85 -0.19318068875345773 2214 86-87 -0.16806308256985858 2214 88-89 -0.16222546395115955 2214 90-91 -0.07984507940800967 2214 92-93 -0.12023627947688453 2214 94-95 -0.18549811303569186 2214 96-97 -0.20538819459746804 2214 98-99 -0.23854660257150329 2214 100 -0.2595479308358577 2215 1 -0.18242655430378818 2215 2 -0.16348486293557585 2215 3 -0.24639725742655827 2215 4 -0.147616257156983 2215 5 -0.1790112079639954 2215 6 -0.1894305032098842 2215 7 -0.19626869844905315 2215 8 -0.18857613253821626 2215 9 -0.23021757132733 2215 10-11 -0.2330887982019405 2215 12-13 -0.22110939480057112 2215 14-15 -0.3533554066157336 2215 16-17 -0.3757331489321558 2215 18-19 -0.35909347737104724 2215 20-21 -0.35719339483065937 2215 22-23 -0.3161060794473869 2215 24-25 -0.3713944876337294 2215 26-27 -0.4290705916814943 2215 28-29 -0.3846420125979577 2215 30-31 -0.37292145210922456 2215 32-33 -0.3510739294252616 2215 34-35 -0.3615437744133416 2215 36-37 -0.468838689203416 2215 38-39 -0.46771213124724653 2215 40-41 -0.45533480471063115 2215 42-43 -0.4135175426709736 2215 44-45 -0.4150783998200964 2215 46-47 -0.44673322801476445 2215 48-49 -0.4514931749699187 2215 50-51 -0.42083029239478265 2215 52-53 -0.35611490693596437 2215 54-55 -0.4198049116163034 2215 56-57 -0.4012656185570691 2215 58-59 -0.4830739922339333 2215 60-61 -0.44764872252358145 2215 62-63 -0.5041022628221583 2215 64-65 -0.46872038303972374 2215 66-67 -0.4277622478550285 2215 68-69 -0.3811281658232062 2215 70-71 -0.3620588372775231 2215 72-73 -0.431794837906466 2215 74-75 -0.3486909201254278 2215 76-77 -0.37492174180059124 2215 78-79 -0.3692615224302358 2215 80-81 -0.39431621502562564 2215 82-83 -0.45129149651580036 2215 84-85 -0.4820139947547446 2215 86-87 -0.3832872695604834 2215 88-89 -0.42609383532281697 2215 90-91 -0.34807557861898175 2215 92-93 -0.42193562895808157 2215 94-95 -0.32949639217545723 2215 96-97 -0.36739640606728585 2215 98-99 -0.42271987253320376 2215 100 -0.4542970084473943 2216 1 -0.18811596101136274 2216 2 -0.15273504060846932 2216 3 -0.17978863949659996 2216 4 -0.16431232421928144 2216 5 -0.15986361395175663 2216 6 -0.20959000942453088 2216 7 -0.18940050318970947 2216 8 -0.19288848176409346 2216 9 -0.24821212895915323 2216 10-11 -0.2698317911899366 2216 12-13 -0.30583979292573105 2216 14-15 -0.3729290509319867 2216 16-17 -0.3486653093946188 2216 18-19 -0.38076586667915535 2216 20-21 -0.39060407393707663 2216 22-23 -0.33793375093035394 2216 24-25 -0.3364977601079957 2216 26-27 -0.40966507713886813 2216 28-29 -0.40193496599484746 2216 30-31 -0.3202996531696414 2216 32-33 -0.35605720247838235 2216 34-35 -0.368054230991369 2216 36-37 -0.31481688942062647 2216 38-39 -0.3647854596436062 2216 40-41 -0.3026231181587704 2216 42-43 -0.2865978521847268 2216 44-45 -0.30403463810699094 2216 46-47 -0.2786509990526653 2216 48-49 -0.3191039113008429 2216 50-51 -0.2737457971276598 2216 52-53 -0.4036249568347898 2216 54-55 -0.39109220910709297 2216 56-57 -0.3403101434916067 2216 58-59 -0.27871453134766 2216 60-61 -0.2728364313540368 2216 62-63 -0.394404137523253 2216 64-65 -0.47053405947098526 2216 66-67 -0.4201600017643159 2216 68-69 -0.40475452962002834 2216 70-71 -0.348727530693246 2216 72-73 -0.40191918557034967 2216 74-75 -0.4263224929256211 2216 76-77 -0.41977721001779855 2216 78-79 -0.42474941839693514 2216 80-81 -0.40089743310105064 2216 82-83 -0.3596180680050196 2216 84-85 -0.4305644608633443 2216 86-87 -0.36060721260032835 2216 88-89 -0.4367786220224801 2216 90-91 -0.49160653062092763 2216 92-93 -0.49183140900900923 2216 94-95 -0.48661762530792885 2216 96-97 -0.5460525791419357 2216 98-99 -0.552550014173562 2216 100 -0.5350770690652702 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 21.0 3 6.0 4 6.0 5 23.0 6 32.0 7 136.0 8 261.0 9 524.0 10 993.0 11 1611.0 12 2646.0 13 4012.0 14 4715.0 15 6230.0 16 7568.0 17 10752.0 18 12422.0 19 6582.0 20 4533.0 21 4152.0 22 4268.0 23 4606.0 24 5255.0 25 6410.0 26 8192.0 27 10820.0 28 14015.0 29 17891.0 30 23207.0 31 29892.0 32 37913.0 33 49670.0 34 70206.0 35 104567.0 36 189571.0 37 428583.0 38 794702.0 39 404305.0 40 1793.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.68453971415858 20.058412947675254 13.592629546511839 23.664417791654323 2 15.601943335977777 20.471528416900867 40.26967160232611 23.656856644795244 3 17.885405924773607 25.789078200394894 30.45139707667996 25.874118798151546 4 12.066744565829485 17.11996881687127 39.44067462552089 31.372611991778353 5 12.22317345069518 37.98016263367302 36.142175124192505 13.654488791439295 6 30.301439890193528 38.83022669581935 17.689884694402775 13.178448719584354 7 26.473291214474038 32.90998908534678 23.015180650488695 17.601539049690487 8 24.847431947081585 36.2788616698723 20.612964589078096 18.26074179396802 9 24.97621080722241 16.15944984164734 21.6159405848688 37.248398766261445 10-11 23.553112465369086 26.728741100996334 29.351572922612622 20.36657351102195 12-13 24.686319201474998 24.1451618083042 29.48551113879735 21.683007851423458 14-15 21.738958287965474 26.01009201151739 26.99520695442537 25.255742746091762 16-17 20.701041776420055 29.531428274790006 27.449525172469087 22.31800477632085 18-19 20.45329264842973 27.94755483641352 30.40523448591096 21.193918029245793 20-21 21.8375111247196 27.298621128674565 29.98034394575492 20.883523800850913 22-23 21.35956050965922 27.114156406209617 30.15033517187526 21.375947912255906 24-25 21.160780197319394 27.29463200804552 30.088677583492956 21.455910211142132 26-27 20.977696420024213 27.152688325309004 30.188162534842473 21.68145271982431 28-29 20.987926969670706 27.255208014841514 30.252282912678506 21.50458210280927 30-31 21.290494720951376 27.733201882284003 29.79546119939404 21.180842197370584 32-33 20.925515080566505 27.426640640431906 30.29056029872979 21.357283980271795 34-35 21.070339902801955 27.16776407103807 30.288998548672268 21.472897477487702 36-37 21.48213599895473 27.66807400143681 29.567206064341462 21.282583935267 38-39 21.34100658530609 27.808719492532415 29.68862663219378 21.161647289967714 40-41 21.126232957677455 27.54405784898185 29.845615507694152 21.48409368564655 42-43 21.159711599755575 27.418149999274117 29.715308362049736 21.70683003892057 44-45 21.397669516970506 27.669723737413065 29.494903635622155 21.437703109994278 46-47 21.46638651950142 28.03220372611567 29.071801348912118 21.429608405470788 48-49 21.424857165859176 27.879042238080213 28.92853827673419 21.767562319326412 50-51 21.50303265465395 27.83942657817043 28.74658339679318 21.910957370382445 52-53 21.566294530223384 27.866570234099736 28.734243371690795 21.832891863986088 54-55 21.656876033559588 28.285383207271508 28.532161712839475 21.525579046329426 56-57 21.396723668344116 27.97290121688925 28.66715410865645 21.96322100611018 58-59 21.272619529970424 27.578790290401923 29.085637134632975 22.06295304499468 60-61 21.580042330025503 28.0309719232534 28.601868556956145 21.787117189764952 62-63 21.421337729109833 27.945757560959944 28.693483894837467 21.939420815092753 64-65 21.42281149324862 27.63877469049853 28.866684175864492 22.07172964038835 66-67 21.29078067537596 27.781968109922637 28.660442098738304 22.2668091159631 68-69 21.8452043410569 27.879289266094965 28.573477389998708 21.70202900284943 70-71 21.77583299568737 27.738506729383026 28.56597030211285 21.91968997281675 72-73 21.535196573289102 27.874382969213407 28.632978067017778 21.957442390479716 74-75 21.587892071097965 27.921410028379885 28.518526991244077 21.97217090927808 76-77 21.662441142919487 27.92173740514568 28.36536240740032 22.050459044534513 78-79 21.750075117978117 28.054090223400646 28.411511021776075 21.78432363684516 80-81 21.635517451787013 27.7786723012849 28.560999097704403 22.024811149223677 82-83 21.514489753810786 27.87946270471592 28.63103908381942 21.975008457653882 84-85 21.534905553715184 27.79842514004059 28.501542613120197 22.165126693124034 86-87 21.641165291146812 27.95724303588083 28.348980238267018 22.052611434705334 88-89 21.351894676709314 27.902792714336904 28.45504884538962 22.290263763564163 90-91 21.290278302954473 27.71278784907203 28.578147062233057 22.418786785740448 92-93 21.396269420523755 27.920571154933715 28.355487942893305 22.327671481649226 94-95 21.207480599011035 27.66660214334735 28.56819821563693 22.55771904200468 96-97 21.17935575319659 27.665024920939068 28.559490754539475 22.596128571324876 98-99 21.23454498142065 27.71376917352756 28.42324440953355 22.62844143551824 100 21.35554625837681 27.638004813709614 28.318971831748048 22.68747709616553 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 32.0 1 28.5 2 89.0 3 162.0 4 116.0 5 80.0 6 220.5 7 498.0 8 857.0 9 1003.5 10 786.0 11 658.0 12 733.0 13 882.0 14 1048.0 15 1198.0 16 1396.5 17 1614.5 18 1926.5 19 2362.0 20 2892.5 21 3647.5 22 4760.5 23 6605.5 24 8912.5 25 11422.5 26 14951.0 27 19640.0 28 25318.0 29 31808.0 30 38476.5 31 45053.5 32 51791.0 33 58926.5 34 65965.5 35 71293.5 36 75475.0 37 79344.0 38 82297.0 39 83853.5 40 84452.0 41 84779.0 42 83240.0 43 81297.5 44 79661.5 45 77671.0 46 76190.5 47 74327.0 48 72021.5 49 69651.5 50 67390.5 51 64579.5 52 60250.5 53 56924.5 54 54770.5 55 52326.0 56 49093.5 57 44553.5 58 40354.0 59 36773.0 60 32595.0 61 27559.0 62 23023.5 63 19058.5 64 15267.5 65 12123.0 66 9387.5 67 6939.0 68 5243.0 69 4057.0 70 2895.5 71 1977.0 72 1397.0 73 1027.5 74 769.0 75 509.5 76 299.5 77 215.0 78 151.0 79 85.0 80 44.5 81 19.5 82 14.0 83 7.5 84 4.0 85 3.0 86 1.5 87 1.5 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.043641015691848674 2 0.23949767079276632 3 0.0025955846026401936 4 0.002947528277574457 5 3.0795071556748057E-4 6 4.3992959366782944E-5 7 0.0 8 3.0795071556748057E-4 9 0.0 10-11 5.939049514515697E-4 12-13 0.0 14-15 2.6395775620069765E-4 16-17 1.0998239841695735E-4 18-19 5.279155124013953E-4 20-21 0.0 22-23 1.5397535778374029E-4 24-25 0.0010998239841695735 26-27 1.3197887810034882E-4 28-29 3.5194367493426355E-4 30-31 6.598943905017441E-5 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 6.598943905017441E-5 68-69 1.7597183746713177E-4 70-71 0.0 72-73 3.2994719525087203E-4 74-75 8.798591873356589E-5 76-77 0.0 78-79 0.0 80-81 0.0 82-83 8.798591873356589E-5 84-85 0.0 86-87 4.839225530346123E-4 88-89 0.0022656374073893215 90-91 0.0020456726105554067 92-93 5.499119920847868E-4 94-95 4.839225530346123E-4 96-97 1.0998239841695735E-4 98-99 1.0998239841695735E-4 100 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2273091.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 70.11945076305338 #Duplication Level Percentage of deduplicated Percentage of total 1 80.03160285725815 56.11772036037755 2 13.111717143893623 18.387728093806636 3 3.524257577393106 7.4135701702300105 4 1.3250924803202422 3.7165902772123 5 0.6373912165256652 2.2346761011987035 6 0.3591575761567129 1.5110359186498938 7 0.2263739305025517 1.111125097173878 8 0.15464843267266717 0.867509052829957 9 0.11158033873324327 0.7041556861137422 >10 0.49355947805561445 5.906238593429756 >50 0.019209206668513872 0.9206594020796455 >100 0.005095848107604591 0.6452978917901826 >500 1.2556548491456863E-4 0.06695885247526737 >1k 1.8834822737038735E-4 0.39673450263240406 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3973 0.17478402756422862 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3351 0.14742040683808963 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 4.399295936678294E-5 0.0 5 0.0 0.0 0.0 4.399295936678294E-5 0.0 6 0.0 0.0 0.0 4.399295936678294E-5 0.0 7 0.0 0.0 0.0 4.399295936678294E-5 0.0 8 0.0 0.0 0.0 4.399295936678294E-5 0.0 9 0.0 0.0 0.0 4.399295936678294E-5 0.0 10-11 0.0 0.0 0.0 4.399295936678294E-5 0.0 12-13 0.0 0.0 0.0 4.399295936678294E-5 4.399295936678294E-5 14-15 0.0 0.0 0.0 4.399295936678294E-5 4.399295936678294E-5 16-17 0.0 0.0 0.0 8.798591873356587E-5 4.399295936678294E-5 18-19 0.0 0.0 0.0 8.798591873356587E-5 4.399295936678294E-5 20-21 0.0 0.0 0.0 1.0998239841695735E-4 8.798591873356587E-5 22-23 0.0 0.0 0.0 1.3197887810034882E-4 8.798591873356587E-5 24-25 0.0 0.0 0.0 1.5397535778374029E-4 8.798591873356587E-5 26-27 0.0 0.0 0.0 3.0795071556748057E-4 8.798591873356587E-5 28-29 0.0 0.0 0.0 6.818908701851356E-4 8.798591873356587E-5 30-31 0.0 0.0 0.0 0.002661574041690368 8.798591873356587E-5 32-33 0.0 0.0 0.0 0.00822668340158841 8.798591873356587E-5 34-35 0.0 0.0 0.0 0.01660734216096056 8.798591873356587E-5 36-37 0.0 0.0 0.0 0.03066309267864771 8.798591873356587E-5 38-39 0.0 0.0 0.0 0.05582706543644755 8.798591873356587E-5 40-41 0.0 0.0 0.0 0.08682010531034613 8.798591873356587E-5 42-43 0.0 0.0 0.0 0.11790113110297828 8.798591873356587E-5 44-45 0.0 0.0 0.0 0.15355742466975586 8.798591873356587E-5 46-47 0.0 0.0 0.0 0.1985842185816582 8.798591873356587E-5 48-49 0.0 0.0 0.0 0.24867020282074057 1.0998239841695735E-4 50-51 0.0 0.0 0.0 0.30748878949412933 1.3197887810034882E-4 52-53 0.0 0.0 0.0 0.36659333040340225 1.3197887810034882E-4 54-55 0.0 0.0 0.0 0.43007517076967006 1.3197887810034882E-4 56-57 0.0 0.0 0.0 0.5073488038974243 1.3197887810034882E-4 58-59 0.0 0.0 0.0 0.595884634623075 1.3197887810034882E-4 60-61 0.0 0.0 0.0 0.6914153458880441 1.3197887810034882E-4 62-63 0.0 0.0 0.0 0.7906414657398231 1.3197887810034882E-4 64-65 0.0 0.0 0.0 0.8972144098058547 1.3197887810034882E-4 66-67 0.0 0.0 0.0 1.0193168685283607 1.3197887810034882E-4 68-69 0.0 0.0 0.0 1.156948841907341 1.5397535778374029E-4 70-71 0.0 0.0 0.0 1.3082186326900243 1.7597183746713175E-4 72-73 0.0 0.0 0.0 1.4735001810310278 1.7597183746713175E-4 74-75 0.0 0.0 0.0 1.6426091168369414 1.7597183746713175E-4 76-77 0.0 2.199647968339147E-5 0.0 1.8199667325241269 1.7597183746713175E-4 78-79 0.0 4.399295936678294E-5 0.0 2.025237880929536 1.979683171505232E-4 80-81 0.0 4.399295936678294E-5 0.0 2.2562449105645133 2.199647968339147E-4 82-83 0.0 4.399295936678294E-5 0.0 2.4968424053414493 2.199647968339147E-4 84-85 0.0 4.399295936678294E-5 0.0 2.733634509133158 2.199647968339147E-4 86-87 0.0 4.399295936678294E-5 0.0 2.9878258283544303 2.199647968339147E-4 88 0.0 4.399295936678294E-5 0.0 3.1995199488273895 2.199647968339147E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3235 0.0 53.48904 1 GTATCAA 5985 0.0 42.304173 1 TCAACGC 7095 0.0 35.041996 4 ATCAACG 7210 0.0 34.680157 3 CAACGCA 7230 0.0 34.321922 5 TATCAAC 7510 0.0 33.320908 2 AACGCAG 7550 0.0 32.99172 6 ACGCAGA 8720 0.0 28.457285 7 CGCAGAG 8805 0.0 28.075815 8 GTACATG 6095 0.0 25.295723 1 GCAGAGT 9770 0.0 24.965992 9 TACATGG 6230 0.0 23.676558 2 ACATGGG 6370 0.0 23.02077 3 GTGGTAT 1665 0.0 22.937382 1 GAGTACT 6845 0.0 21.284071 12-13 AGAGTAC 9140 0.0 21.15922 10-11 CAGAGTA 9600 0.0 21.148935 10-11 TGGTATC 1580 0.0 20.58042 2 GTCGTAC 115 0.0038878506 20.499592 1 CATGGGG 4525 0.0 19.526518 4 >>END_MODULE