FastQCFastQC Report
Wed 25 May 2016
SRR1033086_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033086_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences239699
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA19890.8297906958310215No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT10890.4543197927400615No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT6690.27910003796428023No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT4960.20692618659235124No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC3320.13850704425133187No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC3250.13558671500506886No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA3180.13266638575880585No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG2990.12473977780466336No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT2950.12307101823537019No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC2910.12140225866607704No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT2840.11848192941981402No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA503.0176128E-546.95939694
CCGTATC853.9649785E-738.67244394
GTACAAG1302.2919266E-1036.15281
GTTATAG600.00415504931.3324261
TACAAGA1401.8821083E-830.2071022
CAACGCC552.9910025E-629.87389686-87
GCCGTAT951.4551915E-1129.65237492-93
TCGCTCT804.7763565E-429.3680154
TGGGACA650.00615171428.9161994
TCTGTCG1154.138472E-628.6437788
CTGTCGC1057.335986E-526.8900789
GTCTAGA700.00882354326.8563671
TACATAG700.0088325426.8507585
TGTGGCG350.00885924126.83954480-81
ACAACAC4050.023.2043575
CAACACA7200.021.5365456
GCTTTAG1100.00305306421.3630181
TTGTACT1100.003056767921.3585554
CATACAA1100.003056767921.3585555
CGGGGCG550.003079783421.3362784-85
Produced by FastQC (version 0.11.5)