##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1033086_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 239699 Sequences flagged as poor quality 0 Sequence length 100 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.224239567123767 33.0 31.0 34.0 24.0 34.0 2 30.321390577349092 33.0 31.0 34.0 25.0 34.0 3 30.478846386509748 34.0 31.0 34.0 25.0 34.0 4 33.52815823178236 37.0 35.0 37.0 26.0 37.0 5 33.851638930491994 37.0 35.0 37.0 30.0 37.0 6 33.752251782443814 37.0 35.0 37.0 28.0 37.0 7 33.790216062645236 37.0 35.0 37.0 29.0 37.0 8 33.65627307581592 37.0 35.0 37.0 27.0 37.0 9 35.03278278173877 39.0 37.0 39.0 23.0 39.0 10-11 35.20083521416443 39.0 37.0 39.0 27.0 39.0 12-13 35.17625021380982 39.0 37.0 39.0 27.0 39.0 14-15 36.515246204614954 41.0 38.0 41.0 27.0 41.0 16-17 36.64288962407019 40.5 38.0 41.0 28.0 41.0 18-19 36.70184689965332 40.5 38.0 41.0 29.5 41.0 20-21 36.756490431749825 40.0 38.0 41.0 30.0 41.0 22-23 36.75251461207598 40.0 38.0 41.0 30.0 41.0 24-25 36.746033567098735 40.0 38.0 41.0 29.5 41.0 26-27 36.504755964772485 40.0 38.0 41.0 29.0 41.0 28-29 36.36780503881952 40.0 38.0 41.0 29.0 41.0 30-31 36.33528925861184 40.0 38.0 41.0 29.0 41.0 32-33 36.158381970721614 40.0 38.0 41.0 28.5 41.0 34-35 35.96768447094064 40.0 38.0 41.0 27.0 41.0 36-37 35.7910170672385 40.0 37.5 41.0 25.5 41.0 38-39 35.62093292003721 40.0 37.0 41.0 24.5 41.0 40-41 35.493043358545506 40.0 37.0 41.0 23.5 41.0 42-43 35.34296555263059 40.0 37.0 41.0 20.5 41.0 44-45 35.216219091443854 40.0 37.0 41.0 17.0 41.0 46-47 35.08099950354403 40.0 36.5 41.0 12.5 41.0 48-49 34.94410907012545 40.0 36.0 41.0 4.5 41.0 50-51 34.814686335779456 40.0 36.0 41.0 2.0 41.0 52-53 34.68030321361374 40.0 35.5 41.0 2.0 41.0 54-55 34.52531508266618 40.0 35.0 41.0 2.0 41.0 56-57 34.35601525246246 40.0 35.0 41.0 2.0 41.0 58-59 34.169735793641195 39.5 35.0 41.0 2.0 41.0 60-61 33.943009774759176 39.0 35.0 41.0 2.0 41.0 62-63 33.71657578880179 39.0 34.5 41.0 2.0 41.0 64-65 33.461601842310564 38.5 34.0 41.0 2.0 41.0 66-67 33.152956416171946 38.0 34.0 40.0 2.0 41.0 68-69 32.80607970830083 37.0 34.0 40.0 2.0 41.0 70-71 32.44435521216192 37.0 33.0 39.5 2.0 41.0 72-73 32.10293117618346 36.0 33.0 39.0 2.0 41.0 74-75 31.756087843503728 36.0 33.0 39.0 2.0 41.0 76-77 31.37747132862465 35.0 32.5 38.5 2.0 40.0 78-79 30.976856390723366 35.0 32.0 37.0 2.0 39.0 80-81 30.58860487528108 35.0 32.0 37.0 2.0 39.0 82-83 30.341997672080403 35.0 32.0 36.5 2.0 39.0 84-85 30.108494403397593 35.0 32.0 36.0 2.0 37.0 86-87 29.931651779940676 35.0 32.0 36.0 2.0 37.0 88-89 29.666097897780134 35.0 31.5 35.0 2.0 37.0 90-91 29.408627069783353 35.0 31.0 35.0 2.0 36.0 92-93 29.140419859907635 35.0 31.0 35.0 2.0 36.0 94-95 28.898685434649288 35.0 31.0 35.0 2.0 36.0 96-97 28.693123459004838 35.0 31.0 35.0 2.0 35.5 98-99 28.50545684379159 35.0 30.0 35.0 2.0 35.0 100 28.339926324265015 35.0 30.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.22862724210818897 1101 2 0.19397654941302278 1101 3 0.11632649644084125 1101 4 0.08309402040141833 1101 5 0.0655216706461843 1101 6 0.142279816103823 1101 7 0.10622209690249917 1101 8 0.13533493291215137 1101 9 0.4108201744372977 1101 10-11 0.18279746567490918 1101 12-13 0.07052186135459948 1101 14-15 0.18436581696238363 1101 16-17 -0.0245754126697193 1101 18-19 0.020809461132337503 1101 20-21 -0.011053310945193573 1101 22-23 -0.09932719990160876 1101 24-25 0.14827148587814776 1101 26-27 0.2584815248233099 1101 28-29 0.3318722650730237 1101 30-31 0.22424032967845875 1101 32-33 0.41364944472284293 1101 34-35 0.28710041121492935 1101 36-37 0.29088145528011466 1101 38-39 0.26914279434233634 1101 40-41 0.4813664946996994 1101 42-43 0.4352402277622289 1101 44-45 0.4846125279383102 1101 46-47 0.3750769212986853 1101 48-49 0.3068157800895932 1101 50-51 0.14975810529272593 1101 52-53 0.1421754923716705 1101 54-55 0.14507715514376684 1101 56-57 -0.11458075101714371 1101 58-59 0.021039950358094472 1101 60-61 -0.008514621912091513 1101 62-63 0.3095365830699137 1101 64-65 0.09667043166528089 1101 66-67 0.08684937508357393 1101 68-69 0.32303465013807653 1101 70-71 0.49511029971162657 1101 72-73 0.36640899794720383 1101 74-75 0.40754260239872764 1101 76-77 0.2614555801238012 1101 78-79 0.1073901372039252 1101 80-81 0.003723430321237231 1101 82-83 0.0019609112155194452 1101 84-85 0.08077103240322359 1101 86-87 -0.013590996993318782 1101 88-89 -0.02590116158138045 1101 90-91 -0.12440220717627071 1101 92-93 -0.04412238656479417 1101 94-95 0.17441664700319492 1101 96-97 0.05973932536010906 1101 98-99 -0.028171549469991675 1101 100 -0.17264277714374643 1102 1 0.21787055693289759 1102 2 0.4477925009033932 1102 3 0.25684876487008523 1102 4 -0.08517956647682468 1102 5 0.29754546080950917 1102 6 0.41557628707471395 1102 7 0.3370261765437874 1102 8 0.2096211118494935 1102 9 0.3315273742142608 1102 10-11 0.284376119579143 1102 12-13 0.376918952635414 1102 14-15 0.5494193680624448 1102 16-17 0.5704923103049779 1102 18-19 0.4691111594340427 1102 20-21 0.5672521045695262 1102 22-23 0.3913565349681747 1102 24-25 0.7113886013208415 1102 26-27 0.6324680034609429 1102 28-29 0.5181394862239657 1102 30-31 0.7069494809922716 1102 32-33 0.9056283960041185 1102 34-35 0.8340999233307187 1102 36-37 1.1282882345473553 1102 38-39 0.8896966590822473 1102 40-41 0.5789433364625864 1102 42-43 0.5165194136925848 1102 44-45 0.4898166261656627 1102 46-47 0.4610258560848308 1102 48-49 0.4452703487767238 1102 50-51 0.566533190672466 1102 52-53 0.4397616271671083 1102 54-55 0.550613479284749 1102 56-57 0.6217933400081108 1102 58-59 0.693843890603894 1102 60-61 0.5458728510800341 1102 62-63 0.6626776280714282 1102 64-65 0.361690295522358 1102 66-67 0.5504497282188083 1102 68-69 0.42090189916812193 1102 70-71 0.10038293405402499 1102 72-73 0.1071101572530182 1102 74-75 -0.027714001462531 1102 76-77 -0.012415897236049744 1102 78-79 -0.009463938952475104 1102 80-81 -0.11759130145861008 1102 82-83 -0.05120104310922713 1102 84-85 -0.019192957095651053 1102 86-87 0.06833289307010304 1102 88-89 0.13807439774301855 1102 90-91 0.21296972321891872 1102 92-93 0.12176986072280016 1102 94-95 0.006067554235499983 1102 96-97 0.05971609277873924 1102 98-99 0.25913416806828593 1102 100 -0.14758643813391714 1103 1 -0.5172235737426263 1103 2 -0.816116690680218 1103 3 -0.6538599837456402 1103 4 -0.6535959562885623 1103 5 -0.901867606743096 1103 6 -0.8002376664136577 1103 7 -0.36230937162896737 1103 8 -0.4939890764118573 1103 9 -0.6069886833715614 1103 10-11 -0.6105242126467729 1103 12-13 -0.7311098402770995 1103 14-15 -0.6635595909630325 1103 16-17 -0.6535497716440801 1103 18-19 -0.5632697929469188 1103 20-21 -0.6347246396165218 1103 22-23 -0.3846069201813265 1103 24-25 -0.42351173090506933 1103 26-27 -0.4151898038480226 1103 28-29 -0.2136988304980676 1103 30-31 -0.15939603395789703 1103 32-33 -0.5489729328995381 1103 34-35 -0.4497760456615225 1103 36-37 -0.5851558407571815 1103 38-39 -0.4417429865434457 1103 40-41 -0.3072582139250102 1103 42-43 -0.33375744123543427 1103 44-45 -0.40262523429944963 1103 46-47 -0.27188112065935854 1103 48-49 -0.2748625415887247 1103 50-51 -0.26528851475389104 1103 52-53 -0.2745960693998839 1103 54-55 -0.3121606070939933 1103 56-57 -0.14967399111039015 1103 58-59 -0.03802764870950881 1103 60-61 -0.09713933053679824 1103 62-63 -0.07076644723311176 1103 64-65 -0.050264300269454054 1103 66-67 -0.27523687200267233 1103 68-69 -0.053095885992455294 1103 70-71 0.007581162182482615 1103 72-73 0.1814789280171425 1103 74-75 0.10672675158287603 1103 76-77 0.21243460110282086 1103 78-79 0.09585167566546815 1103 80-81 0.07434114593895558 1103 82-83 0.09851102776563536 1103 84-85 0.29982697645916545 1103 86-87 0.320348396946077 1103 88-89 0.01787506219484669 1103 90-91 0.17219452942046587 1103 92-93 0.1914487090062984 1103 94-95 0.19787818546473446 1103 96-97 0.01093979156057756 1103 98-99 -0.12957015086859158 1103 100 -0.23984557434654263 1104 1 0.6480705439044314 1104 2 0.46695520474461816 1104 3 0.8224145260582816 1104 4 1.0573844358683004 1104 5 1.0430343540412181 1104 6 1.104206778030786 1104 7 1.1050108898089377 1104 8 1.2011743222809557 1104 9 0.8405930159748394 1104 10-11 0.8949986631702274 1104 12-13 1.15973983292551 1104 14-15 1.1865711279912148 1104 16-17 1.1344240845650688 1104 18-19 1.033070076334127 1104 20-21 0.8955694780305379 1104 22-23 1.1771251059624603 1104 24-25 1.1749523213825128 1104 26-27 0.9003984314460922 1104 28-29 1.0892423459725151 1104 30-31 1.1939372993754347 1104 32-33 1.2713211847475208 1104 34-35 1.1243416013972976 1104 36-37 1.4098768389813827 1104 38-39 1.4245315232604767 1104 40-41 1.7237221782318528 1104 42-43 1.457419029352799 1104 44-45 1.8825054846548 1104 46-47 1.7154718205171164 1104 48-49 1.6354642557968901 1104 50-51 1.6702343610631019 1104 52-53 1.7209268064171042 1104 54-55 1.8011400465007767 1104 56-57 1.7907508454909191 1104 58-59 1.9056324820094446 1104 60-61 1.756716878613524 1104 62-63 1.7628936834858422 1104 64-65 1.823886026527937 1104 66-67 1.6436846966247813 1104 68-69 1.8359237218506834 1104 70-71 1.8739210314635315 1104 72-73 1.8634723920694256 1104 74-75 1.8394064901449596 1104 76-77 1.488316954043995 1104 78-79 1.6540216103059855 1104 80-81 1.7307790544356898 1104 82-83 1.716550243451909 1104 84-85 1.7176374339755078 1104 86-87 1.4473418609983604 1104 88-89 1.531208395528182 1104 90-91 1.4063121764792896 1104 92-93 1.5302068789409375 1104 94-95 1.4203945253340144 1104 96-97 1.746528026854694 1104 98-99 1.6841553393274857 1104 100 1.602049850490058 1105 1 -0.25685759945973885 1105 2 -0.038203733212363034 1105 3 -0.16257412815711092 1105 4 -0.09141989298490216 1105 5 -0.4126589024898806 1105 6 -0.48415458036024717 1105 7 -0.11453489091680069 1105 8 0.126505483429888 1105 9 0.319579071326757 1105 10-11 0.037858576670736 1105 12-13 0.14163991244298302 1105 14-15 -0.07618075812425218 1105 16-17 -0.10560714058571818 1105 18-19 0.36143841674645927 1105 20-21 0.04454835741611873 1105 22-23 -0.025838374187259205 1105 24-25 -0.3912120276409681 1105 26-27 -0.18740048634060713 1105 28-29 -0.36063255947927786 1105 30-31 -0.16981146422496352 1105 32-33 -0.07450705752862063 1105 34-35 -0.2063734739503431 1105 36-37 -0.19902329871959523 1105 38-39 0.09013634085516742 1105 40-41 0.13525415007103447 1105 42-43 -0.07846565092881264 1105 44-45 0.026529068371175413 1105 46-47 0.10166487340592312 1105 48-49 0.08751134565163454 1105 50-51 -0.04125785204370658 1105 52-53 0.12244151714808282 1105 54-55 0.26711238993864583 1105 56-57 0.20145855092719955 1105 58-59 0.14551339975735544 1105 60-61 0.12163416105568814 1105 62-63 -0.014782273148050251 1105 64-65 0.09346962653568625 1105 66-67 -0.17532094717576996 1105 68-69 0.2003561417028905 1105 70-71 -0.06205286353462469 1105 72-73 0.08431275591031806 1105 74-75 0.01988204337317967 1105 76-77 0.059140832556828116 1105 78-79 0.07982793679306255 1105 80-81 0.41246676413875605 1105 82-83 0.23844178938779237 1105 84-85 0.03892193194878146 1105 86-87 -0.002193640640474115 1105 88-89 -0.03209526421464659 1105 90-91 0.11169996957872286 1105 92-93 -0.15280450563267323 1105 94-95 0.22697314095434962 1105 96-97 0.06797737810854798 1105 98-99 -0.34767104107630686 1105 100 -0.1642075921506958 1106 1 -0.2807559120510845 1106 2 0.005624602852119409 1106 3 -0.255899784740663 1106 4 0.048692600925370755 1106 5 0.3274358758439675 1106 6 0.24334442313858062 1106 7 0.6182876432964051 1106 8 0.08372236637421793 1106 9 0.21768793354385707 1106 10-11 0.020147429144273588 1106 12-13 0.18406428907613304 1106 14-15 0.19683841898721965 1106 16-17 0.1681915218882608 1106 18-19 0.3863819483466173 1106 20-21 0.19460371859243253 1106 22-23 0.14755725892315041 1106 24-25 0.22178677655762158 1106 26-27 -0.059742042653994076 1106 28-29 -0.13782818373189798 1106 30-31 -0.05805275037581481 1106 32-33 -0.05427144036222842 1106 34-35 -0.09266161780082172 1106 36-37 0.06449590053635745 1106 38-39 -0.05751413082204948 1106 40-41 -0.03671095024341753 1106 42-43 -0.07505097357374524 1106 44-45 -0.10001329400094505 1106 46-47 -0.02509007588323442 1106 48-49 0.022450891247089544 1106 50-51 -0.10785070380862294 1106 52-53 -0.2126308952705358 1106 54-55 -0.11566806978056121 1106 56-57 0.1470673024219593 1106 58-59 -0.07902267358512916 1106 60-61 0.016691515234349197 1106 62-63 -0.13096545220823685 1106 64-65 -0.0026772355928343927 1106 66-67 0.19523576481324767 1106 68-69 0.1262075965946039 1106 70-71 -0.051622817917014174 1106 72-73 0.14722519667385825 1106 74-75 0.1311048610356096 1106 76-77 0.21004654140132928 1106 78-79 0.20032928760576496 1106 80-81 0.05043567330213605 1106 82-83 0.11428217204424485 1106 84-85 0.20915533466812164 1106 86-87 0.07562202878687074 1106 88-89 0.07697953980678562 1106 90-91 0.25149801200753075 1106 92-93 0.11214522641923708 1106 94-95 0.16884833471846505 1106 96-97 0.2105169059884382 1106 98-99 0.27490746107170594 1106 100 0.3122439778922619 1107 1 0.596109759590707 1107 2 0.4733277537642273 1107 3 0.6389177940321389 1107 4 0.43973737704477145 1107 5 0.22702128214579176 1107 6 0.33198455580856745 1107 7 -0.003976038295633089 1107 8 0.16212203469925868 1107 9 -0.019210905593787686 1107 10-11 0.1850313429087862 1107 12-13 0.03972349305622913 1107 14-15 -0.19300403540746913 1107 16-17 -0.19049421608852413 1107 18-19 0.06256354038641376 1107 20-21 -0.06750241739429441 1107 22-23 -0.18905136462576877 1107 24-25 -4.561753495053722E-4 1107 26-27 -0.03407869273691233 1107 28-29 0.29463450283526527 1107 30-31 0.3953261882643204 1107 32-33 0.14574928932267994 1107 34-35 0.3496683987829172 1107 36-37 0.5337330481317082 1107 38-39 0.5354792356787783 1107 40-41 0.6185751194083267 1107 42-43 0.6704092254312286 1107 44-45 0.5195969879227675 1107 46-47 0.5025633237850897 1107 48-49 0.41708190285572044 1107 50-51 0.5561003741349992 1107 52-53 0.6748483750445544 1107 54-55 0.6286727262393441 1107 56-57 0.491159342222943 1107 58-59 0.5553056846238249 1107 60-61 0.5564717805743129 1107 62-63 0.8167335527668911 1107 64-65 0.7647356997305437 1107 66-67 0.7633742391084368 1107 68-69 0.7952374473408739 1107 70-71 0.7728589399602654 1107 72-73 0.8298122613504688 1107 74-75 1.1247823071384317 1107 76-77 1.101879045547264 1107 78-79 0.6125183423321339 1107 80-81 0.5985078126056216 1107 82-83 0.7282332499878557 1107 84-85 0.8803825320147212 1107 86-87 0.7872928413905207 1107 88-89 0.5312083955281821 1107 90-91 0.6816389738649136 1107 92-93 0.7639487090063 1107 94-95 0.9684337410202879 1107 96-97 1.1217731248939096 1107 98-99 1.1232076269091849 1107 100 1.249598870097902 1108 1 0.5143038296715687 1108 2 0.6448087360942658 1108 3 0.4694733495876946 1108 4 0.6445891290663326 1108 5 0.9624360769945497 1108 6 1.1193310667399814 1108 7 0.7118146175893614 1108 8 0.44092556869146904 1108 9 1.03998346397794 1108 10-11 1.0280606930136074 1108 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30-31 0.4088301301219204 2215 32-33 0.4634106591181961 2215 34-35 0.6033207762157886 2215 36-37 0.6127303381046119 2215 38-39 0.5178209454595191 2215 40-41 0.48042840839576684 2215 42-43 0.519753275689915 2215 44-45 0.664725098294781 2215 46-47 0.793207571136648 2215 48-49 0.5560908952183681 2215 50-51 0.5387654394220078 2215 52-53 0.5741215950494833 2215 54-55 0.6622185503339466 2215 56-57 0.39902150038998485 2215 58-59 0.4459930460428936 2215 60-61 0.38079119422299357 2215 62-63 0.37993569615196066 2215 64-65 0.29396703749846864 2215 66-67 0.3794896616263017 2215 68-69 0.4084857843696952 2215 70-71 0.5628657150280176 2215 72-73 0.45834145573332563 2215 74-75 0.5336299297055618 2215 76-77 0.6189091022114681 2215 78-79 0.4390889262197888 2215 80-81 0.3137609537733965 2215 82-83 0.4453772509733227 2215 84-85 0.3479977081664849 2215 86-87 0.44261742872729926 2215 88-89 0.4473207384697915 2215 90-91 0.4167030290509146 2215 92-93 0.4065854421918118 2215 94-95 0.1990200923383476 2215 96-97 0.23806100368178917 2215 98-99 0.24515268726888095 2215 100 0.27516551212795903 2216 1 -0.2415191063886759 2216 2 -0.2990376528795302 2216 3 -0.3717637638143678 2216 4 -0.2674791178005904 2216 5 -0.14038994351285794 2216 6 -0.506217047856957 2216 7 -0.31385576338154664 2216 8 -0.600105914899828 2216 9 -0.5796347314815264 2216 10-11 -0.6785139033684189 2216 12-13 -0.6855771942289905 2216 14-15 -0.6775114809973957 2216 16-17 -0.6591511462145263 2216 18-19 -0.8104175592699434 2216 20-21 -0.7015803097196098 2216 22-23 -0.7474419717277243 2216 24-25 -1.0700581226576418 2216 26-27 -0.9289526904459606 2216 28-29 -0.6179943631441205 2216 30-31 -0.8770248999372825 2216 32-33 -0.8366533452706761 2216 34-35 -0.9515629872422764 2216 36-37 -0.9704479369771093 2216 38-39 -0.7639766635193865 2216 40-41 -0.6915193823098846 2216 42-43 -0.7354756542938574 2216 44-45 -0.7600994611035716 2216 46-47 -0.6648192649892479 2216 48-49 -0.5313573869495514 2216 50-51 -0.3673675525545832 2216 52-53 -0.4034448666507515 2216 54-55 -0.5367482359599762 2216 56-57 -0.46893515949525977 2216 58-59 -0.462220088572046 2216 60-61 -0.33294689067425765 2216 62-63 -0.4360585434530435 2216 64-65 -0.515848492709317 2216 66-67 -0.370374329047344 2216 68-69 -0.3003811093601527 2216 70-71 -0.18073498902025875 2216 72-73 -0.32517055651894466 2216 74-75 -0.36818733776420487 2216 76-77 -0.20674065662913677 2216 78-79 -0.29590090165412164 2216 80-81 -0.3993873763978151 2216 82-83 -0.6751315839182119 2216 84-85 -0.6344513740562974 2216 86-87 -0.7249780635350263 2216 88-89 -0.6351146285268037 2216 90-91 -0.49983296198846716 2216 92-93 -0.7117815315435294 2216 94-95 -0.6927919176280497 2216 96-97 -0.6424450881645107 2216 98-99 -0.4254464395283861 2216 100 -0.2677837186764407 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7029.0 3 788.0 4 538.0 5 585.0 6 1544.0 7 3257.0 8 3538.0 9 2202.0 10 1300.0 11 1247.0 12 1246.0 13 844.0 14 760.0 15 679.0 16 644.0 17 665.0 18 648.0 19 538.0 20 471.0 21 513.0 22 611.0 23 982.0 24 1640.0 25 1748.0 26 1806.0 27 1420.0 28 1508.0 29 1839.0 30 2262.0 31 2731.0 32 3585.0 33 5081.0 34 7220.0 35 11802.0 36 19055.0 37 40342.0 38 72422.0 39 34529.0 40 80.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.92213809281319 22.342619105260084 13.623120653816292 21.112122148110426 2 16.031400895618315 25.17455375587932 36.991206580666166 21.802838767836203 3 18.92478504048752 29.15017948075799 29.904833458552467 22.02020202020202 4 12.65200312250836 21.10406552258183 37.062671414437844 29.181259940471964 5 12.167251169004677 41.202822311289246 33.52580160320641 13.104124916499666 6 26.952542783305013 41.18428029275095 17.164674496181963 14.698502427762076 7 23.61534414950025 37.85185247037801 21.684800307283794 16.84800307283795 8 22.19754065846886 37.869684127017265 20.738220004592996 19.194555209920875 9 22.57043606059088 21.801359453546436 21.30325500609583 34.32494947976685 10-11 21.223514969822357 31.409173752653984 27.403720691361322 19.963590586162336 12-13 22.51226045400738 30.69696166220231 26.634361840498226 20.156416043292086 14-15 20.601469535023085 32.3631785219877 25.090462638356158 21.944889304633065 16-17 19.054318647690696 34.718053195619774 26.02418893587886 20.20343922081067 18-19 19.01258640234317 33.95677325478227 27.732673081299097 19.29796726157546 20-21 21.563109937479776 32.134768132430146 26.50544312120991 19.796678808880166 22-23 20.826168255505284 32.319857421907614 26.420354942651553 20.433619379935557 24-25 21.082972384266792 32.42011133087971 26.315273361541607 20.181642923311884 26-27 20.309251857872006 32.03458138597147 27.184997461552445 20.471169294604074 28-29 20.05869105587547 32.977402273756624 27.05236068701095 19.911545983356955 30-31 20.68369502592664 33.22659296432061 26.35332036305642 19.736391646696337 32-33 19.914084074869592 32.98724828992658 27.215267819309375 19.883399815894446 34-35 20.198361866454146 32.38776949299641 27.086575733425683 20.327292907123752 36-37 20.273426005730634 32.53544082691437 26.945880349514482 20.245252817840516 38-39 19.90941348361511 32.67355458150699 27.174076393237318 20.242955541640576 40-41 19.653961037741166 32.51488794123725 27.493586652723245 20.337564368298338 42-43 19.450335217453016 32.307573493191285 27.321892246165664 20.920199043190035 44-45 19.769235425367825 32.08537621346558 27.437732596122338 20.70765576504426 46-47 19.94234795368633 32.71805811543351 27.111699274235463 20.2278946566447 48-49 19.536707904310855 31.935970677926832 27.25984307848372 21.267478339278593 50-51 19.004522997994187 31.386099556049068 27.59341858916693 22.01595885678982 52-53 19.5760757261086 31.265197925254906 26.828708293762197 22.330018054874294 54-55 19.48401343293239 31.193372864057306 26.99151153883883 22.331102164171472 56-57 20.127318076036023 30.59724700750342 26.668545130083594 22.606889786376964 58-59 20.2067110115774 29.920500581270364 26.955082515345747 22.917705891806488 60-61 20.0745503914313 30.06747520030722 27.090885943327507 22.767088464933977 62-63 19.680455835698783 29.968275171147102 26.909542494573387 23.44172649858073 64-65 19.561496879761233 29.90169682549621 27.039008202366787 23.497798092375767 66-67 19.52179094633715 29.48378384583435 26.958117051851204 24.036308155977302 68-69 19.867216556813865 29.61663529009071 27.206204630932156 23.309943522163273 70-71 20.37010756368463 29.155101240091994 27.320633277541017 23.154157918682355 72-73 19.746851293395803 28.80176974528607 28.445525027913142 23.005853933404985 74-75 19.794810149301078 29.30967556128774 27.890334456117504 23.00517983329368 76-77 20.074503052120832 28.651745187040223 28.103093859237234 23.17065790160171 78-79 20.589579476991332 28.45369989711784 28.532791862918387 22.42392876297244 80-81 21.013576398018422 28.05030737833202 28.46953553109884 22.46658069255072 82-83 20.67856598780852 28.018915371243374 28.99431745852872 22.308201182419392 84-85 20.615196492699734 28.166909765477467 28.577986724509575 22.639907017313227 86-87 20.95359318921626 28.09114431182706 28.328394958684584 22.626867540272098 88-89 20.944048210268885 27.911834302240656 27.971930205285933 23.17218728220452 90-91 21.177135846158983 27.660835308650217 28.076069175040896 23.085959670149904 92-93 21.355017621614177 27.978407523563302 28.032451313639694 22.63412354118283 94-95 20.601203157505964 27.9843668816603 28.23059058215212 23.18383937868162 96-97 20.052543059015996 28.569192823427773 28.390571463150998 22.98769265440523 98-99 20.200114768636862 29.113255777557516 27.637957118263863 23.04867233554176 100 20.22771570595625 28.798774609030996 27.507022208124475 23.466487476888275 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1326.0 1 819.5 2 284.5 3 253.5 4 202.5 5 139.5 6 123.5 7 137.5 8 158.0 9 164.0 10 127.5 11 90.0 12 84.5 13 73.5 14 76.0 15 86.0 16 94.5 17 107.5 18 148.0 19 194.5 20 226.5 21 323.0 22 475.5 23 714.5 24 992.5 25 1251.5 26 1667.5 27 2256.0 28 3099.5 29 3911.5 30 4521.5 31 5283.0 32 6098.0 33 6988.0 34 7858.0 35 8499.0 36 8984.0 37 9280.5 38 9325.0 39 9231.0 40 9134.5 41 8757.0 42 8198.0 43 7932.0 44 7706.0 45 7371.5 46 7356.5 47 8050.5 48 9467.0 49 9375.5 50 7805.0 51 6728.5 52 6020.5 53 5455.0 54 4974.0 55 4667.5 56 4409.5 57 4014.5 58 3410.0 59 2749.0 60 2234.0 61 1868.0 62 1524.0 63 1170.5 64 886.5 65 652.5 66 481.5 67 404.5 68 321.5 69 257.0 70 213.5 71 180.0 72 165.5 73 128.0 74 88.0 75 90.0 76 110.0 77 77.5 78 36.5 79 61.0 80 61.5 81 33.5 82 24.0 83 14.0 84 5.0 85 2.5 86 2.5 87 3.0 88 1.5 89 1.0 90 1.5 91 1.0 92 0.5 93 0.0 94 0.5 95 0.5 96 0.5 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.050479976971117946 2 0.03671271052444941 3 0.049645597186471366 4 0.06174410406384675 5 0.07467699072586868 6 0.07425980083354541 7 0.07551137051051528 8 0.08510673803395091 9 0.07968326943374816 10-11 0.08364657341081941 12-13 0.08740128244172901 14-15 0.09824821964213451 16-17 0.07008790191031251 18-19 0.08114343405687967 20-21 0.07363401599506046 22-23 0.04630807804788505 24-25 0.022111064293134308 26-27 0.1583235641366881 28-29 0.05819798997909879 30-31 0.07384261094122212 32-33 0.06779335750253443 34-35 0.015018836123638397 36-37 0.04568229320940012 38-39 0.062161293956170025 40-41 0.06654178782556455 42-43 0.06383005352546318 44-45 0.06153550911768509 46-47 0.06612459793324128 48-49 0.06362145857930154 50-51 0.06070112933303852 52-53 0.06320426868697825 54-55 0.0577808000867755 56-57 0.05903236976374537 58-59 0.057363610194452214 60-61 0.054026091055865894 62-63 0.057989395032937145 64-65 0.0559034455713207 66-67 0.0577808000867755 68-69 0.056737825355967275 70-71 0.050062787078794656 72-73 0.048602622455663144 74-75 0.0483940275095015 76-77 0.0465166729940467 78-79 0.04359634374778368 80-81 0.03838147009374257 82-83 0.04401353364010697 84-85 0.035252545901317905 86-87 0.032958001493539814 88-89 0.034209571170509684 90-91 0.03128924192424666 92-93 0.032749406547378165 94-95 0.03316659643970146 96-97 0.03587833073980284 98-99 0.03608692568596448 100 0.042553369016975456 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 239699.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 82.86869841396093 #Duplication Level Percentage of deduplicated Percentage of total 1 92.03256513098805 76.26618884103064 2 6.264353929877848 10.38237713146717 3 0.8070614255924653 2.0064038963689015 4 0.24579234531253782 0.8147396694465936 5 0.13696365073226238 0.5674999733103465 6 0.08977670860781245 0.4463807394131316 7 0.06452598587587335 0.37430291243878755 8 0.049543949307593205 0.32845140747260076 9 0.03529716202999099 0.2632526887618833 >10 0.2429438008074938 3.912174006741375 >50 0.01759930452233243 0.9813160243919575 >100 0.012068094529599376 2.095557658882018 >500 5.028372720666407E-4 0.27876875597376616 >1k 0.0010056745441332814 1.2825862943008255 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1989 0.8297906958310215 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1089 0.4543197927400615 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 669 0.27910003796428023 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 496 0.20692618659235124 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 332 0.13850704425133187 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 325 0.13558671500506886 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 318 0.13266638575880585 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 299 0.12473977780466336 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 295 0.12307101823537019 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 291 0.12140225866607704 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 284 0.11848192941981402 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 4.1718989232328877E-4 0.0 14-15 0.0 0.0 0.0 0.0012515696769698664 0.0 16-17 0.0 0.0 0.0 0.001668759569293155 0.0 18-19 0.0 0.0 0.0 0.0018773545154547994 0.0 20-21 0.0 0.0 0.0 0.003754709030909599 0.0 22-23 0.0 0.0 0.0 0.006257848384849332 0.0 24-25 0.0 0.0 0.0 0.012098506877375376 0.0 26-27 0.0 0.0 0.0 0.024405608700912393 0.0 28-29 0.0 0.0 0.0 0.049019812347986434 0.0 30-31 0.0 0.0 0.0 0.11159829619647975 0.0 32-33 0.0 0.0 0.0 0.2307060104547787 0.0 34-35 0.0 0.0 0.0 0.406551550069045 0.0 36-37 0.0 0.0 0.0 0.665209283309484 0.0 38-39 0.0 0.0 0.0 0.9518187393355834 0.0 40-41 0.0 0.0 0.0 1.2459376134235018 0.0 42-43 0.0 0.0 0.0 1.507515675910204 0.0 44-45 0.0 0.0 0.0 1.7763945615125638 0.0 46-47 0.0 0.0 0.0 2.0995081331169505 0.0 48-49 0.0 0.0 0.0 2.410940387736286 0.0 50-51 0.0 4.1718989232328877E-4 0.0 2.7190351232170347 0.0 52-53 0.0 4.1718989232328877E-4 0.0 2.995632021827375 0.0 54-55 0.0 4.1718989232328877E-4 0.0 3.3254206317089348 0.0 56-57 0.0 4.1718989232328877E-4 0.0 3.7204994597390897 0.0 58-59 0.0 4.1718989232328877E-4 0.0 4.1147439079845975 0.0 60-61 0.0 4.1718989232328877E-4 0.0 4.484374152583031 0.0 62-63 0.0 4.1718989232328877E-4 0.0 4.876741246313085 0.0 64-65 0.0 4.1718989232328877E-4 0.0 5.304569480890617 0.0 66-67 0.0 4.1718989232328877E-4 0.0 5.820841972640687 0.0 68-69 0.0 4.1718989232328877E-4 0.0 6.393017909962078 0.0 70-71 0.0 4.1718989232328877E-4 0.0 6.952052365675284 0.0 72-73 0.0 4.1718989232328877E-4 0.0 7.521099378804251 0.0 74-75 0.0 4.1718989232328877E-4 0.0 8.116429355149583 0.0 76-77 0.0 4.1718989232328877E-4 0.0 8.757858814596641 0.0 78-79 0.0 4.1718989232328877E-4 0.0 9.522359292279067 0.0 80-81 0.0 4.1718989232328877E-4 0.0 10.289571504261595 0.0 82-83 0.0 4.1718989232328877E-4 0.0 11.048022728505334 0.0 84-85 0.0 4.1718989232328877E-4 0.0 11.821909978765035 0.0 86-87 0.0 4.1718989232328877E-4 0.0 12.64210530707262 0.0 88 0.0 4.1718989232328877E-4 0.0 13.319621692205642 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTATCA 50 3.0176128E-5 46.959396 94 CCGTATC 85 3.9649785E-7 38.672443 94 GTACAAG 130 2.2919266E-10 36.1528 1 GTTATAG 60 0.004155049 31.332426 1 TACAAGA 140 1.8821083E-8 30.207102 2 CAACGCC 55 2.9910025E-6 29.873896 86-87 GCCGTAT 95 1.4551915E-11 29.652374 92-93 TCGCTCT 80 4.7763565E-4 29.368015 4 TGGGACA 65 0.006151714 28.916199 4 TCTGTCG 115 4.138472E-6 28.643778 8 CTGTCGC 105 7.335986E-5 26.890078 9 GTCTAGA 70 0.008823543 26.856367 1 TACATAG 70 0.00883254 26.850758 5 TGTGGCG 35 0.008859241 26.839544 80-81 ACAACAC 405 0.0 23.204357 5 CAACACA 720 0.0 21.536545 6 GCTTTAG 110 0.003053064 21.363018 1 TTGTACT 110 0.0030567679 21.358555 4 CATACAA 110 0.0030567679 21.358555 5 CGGGGCG 55 0.0030797834 21.33627 84-85 >>END_MODULE