FastQCFastQC Report
Wed 25 May 2016
SRR1033086_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033086_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences239699
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18390.7672122119825281No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGT17700.7384261094122211No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17490.7296651216734321No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT10840.45223384327844507No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9250.38590065039904214No Hit
ATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTT7650.3191502676273159RNA PCR Primer, Index 31 (95% over 22bp)
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT7200.3003767224727679No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT4970.20734337648467455No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4900.2044230472384115No Hit
ACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCT4340.18106041326830735RNA PCR Primer, Index 31 (95% over 24bp)
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3560.14851960166709083No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC3210.1339179554357757No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3200.13350076554345242No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC3160.13183200597415926No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA3050.12724291715860309No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3020.1259913474816332No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT2870.11973349909678388No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG2710.11305846081961127No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2530.10554904275779206No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT2460.10262871351152904No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG2450.10221152361920575No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC2420.10095995394223589No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACTCG200.002161627270.49395
CTATACA555.163778E-542.875651
TAATAGT851.750055E-533.17364
CCTTAGA700.00869211226.9391252
CTCAGCG350.00883470626.85481642-43
TCTATAC700.00882605226.8548163
TATACCA950.001302232824.7347015
CATGGGC2950.023.8962364
GGTATCA25750.023.8105531
ATGGGCA1852.7978604E-722.8628865
TCTAGCA1050.002289228422.4492722
GAGTACT22500.021.82699812-13
TATATAC1303.1514087E-421.690433
GTATCAA33750.021.3806551
AGTACTT22850.021.08132612-13
GTACATG10400.020.407161
AGAGTAC28250.020.2124110-11
TACATGG10200.019.8742072
CATGGGG3553.6379788E-1219.8574374
TAGGTGG1200.00505519919.5816387
Produced by FastQC (version 0.11.5)