##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1033086_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 239699 Sequences flagged as poor quality 0 Sequence length 100 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.58841713982954 34.0 31.0 34.0 31.0 34.0 2 32.55728643006437 34.0 31.0 34.0 31.0 34.0 3 32.62339434040192 34.0 31.0 34.0 31.0 34.0 4 36.17593732139058 37.0 35.0 37.0 35.0 37.0 5 36.18041794083413 37.0 35.0 37.0 35.0 37.0 6 36.27592939478262 37.0 37.0 37.0 35.0 37.0 7 36.21695960350273 37.0 37.0 37.0 35.0 37.0 8 36.23552038181219 37.0 37.0 37.0 35.0 37.0 9 38.009737212086826 39.0 39.0 39.0 35.0 39.0 10-11 37.97766573911447 39.0 38.0 39.0 35.0 39.0 12-13 37.9487148465367 39.0 38.0 39.0 35.0 39.0 14-15 39.326077705789345 41.0 39.0 41.0 36.0 41.0 16-17 39.334325549960575 41.0 39.0 41.0 36.0 41.0 18-19 39.43231928376839 41.0 39.0 41.0 36.0 41.0 20-21 39.45377118803165 41.0 39.0 41.0 36.0 41.0 22-23 39.34490965752882 41.0 39.0 41.0 36.0 41.0 24-25 39.24900604508154 41.0 39.0 41.0 35.5 41.0 26-27 39.067951889661614 41.0 39.0 41.0 35.0 41.0 28-29 38.95246955556761 41.0 39.0 41.0 35.0 41.0 30-31 38.75750003128924 40.0 38.0 41.0 35.0 41.0 32-33 38.47720057238453 40.0 38.0 41.0 35.0 41.0 34-35 38.22574353668559 40.0 38.0 41.0 34.5 41.0 36-37 38.00023779823863 40.0 38.0 41.0 34.0 41.0 38-39 37.76078540169129 40.0 37.0 41.0 33.5 41.0 40-41 37.53502309146054 40.0 37.0 41.0 33.0 41.0 42-43 37.22447319346347 40.0 37.0 41.0 33.0 41.0 44-45 36.780760453735724 40.0 37.0 41.0 31.5 41.0 46-47 36.1821367631905 40.0 37.0 41.0 29.0 41.0 48-49 35.6300526910834 40.0 37.0 41.0 24.0 41.0 50-51 35.30298624524925 40.0 36.0 41.0 20.5 41.0 52-53 35.14778326150714 40.0 36.0 41.0 17.5 41.0 54-55 34.990627829068956 40.0 35.5 41.0 14.5 41.0 56-57 34.820604174402064 40.0 35.0 41.0 11.5 41.0 58-59 34.646498316638784 40.0 35.0 41.0 8.0 41.0 60-61 34.41906098899036 39.0 35.0 41.0 6.5 41.0 62-63 34.20366584758385 39.0 35.0 41.0 2.0 41.0 64-65 33.90574845952632 39.0 35.0 41.0 2.0 41.0 66-67 33.53423877446298 38.0 35.0 40.5 2.0 41.0 68-69 33.11344018957109 37.0 34.0 40.0 2.0 41.0 70-71 32.6444373985707 37.0 34.0 40.0 2.0 41.0 72-73 32.229199954943496 36.0 34.0 39.0 2.0 41.0 74-75 31.89908593694592 36.0 34.0 39.0 2.0 41.0 76-77 31.55178786728355 35.0 33.0 38.5 2.0 40.0 78-79 31.19693448867121 35.0 33.0 37.0 2.0 39.0 80-81 30.873658213008817 35.0 33.0 37.0 2.0 39.0 82-83 30.56690057113296 35.0 33.0 36.0 2.0 39.0 84-85 30.342598425525345 35.0 33.0 36.0 2.0 37.0 86-87 30.149043174981955 35.0 33.0 36.0 2.0 37.0 88-89 29.938222520744766 35.0 33.0 35.0 2.0 37.0 90-91 29.742059833374356 35.0 32.0 35.0 2.0 36.0 92-93 29.553665221799005 35.0 32.0 35.0 2.0 36.0 94-95 29.398074251457036 35.0 32.0 35.0 2.0 36.0 96-97 29.26143621792331 35.0 32.0 35.0 2.0 35.5 98-99 29.132522455245955 35.0 32.0 35.0 2.0 35.0 100 29.023287539789486 35.0 32.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.33609867746491773 1101 2 0.3484453508892926 1101 3 0.3253633446417368 1101 4 0.11240632377845827 1101 5 0.3625462266507711 1101 6 0.1544689302627802 1101 7 0.19355465941848848 1101 8 0.35379534911054833 1101 9 0.46897495528757815 1101 10-11 0.4551652315864487 1101 12-13 0.5223859970388247 1101 14-15 0.5904373139572172 1101 16-17 0.7343550503252629 1101 18-19 0.7170642053828615 1101 20-21 0.698606212579655 1101 22-23 0.7309552979687908 1101 24-25 0.732598557039374 1101 26-27 0.7030881712387682 1101 28-29 0.5848597258281458 1101 30-31 0.8386145780539707 1101 32-33 0.8926995940416944 1101 34-35 0.6494559608988055 1101 36-37 0.4544608450916172 1101 38-39 0.18004633112968804 1101 40-41 0.2534447158027717 1101 42-43 0.4525723750980575 1101 44-45 -0.1570158001641886 1101 46-47 -0.45866638877289745 1101 48-49 -0.36008999226212524 1101 50-51 -0.1930566432849261 1101 52-53 -0.10566473043986235 1101 54-55 -0.09910691888246959 1101 56-57 0.12788916188909383 1101 58-59 0.19196701941857697 1101 60-61 0.17580204268928412 1101 62-63 0.2294302454202395 1101 64-65 -0.0021366319403242073 1101 66-67 0.240118359867175 1101 68-69 0.310317166740802 1101 70-71 0.2194795289066107 1101 72-73 0.2240653157054311 1101 74-75 0.11318321072096893 1101 76-77 0.18250287604139714 1101 78-79 0.062303142832313085 1101 80-81 -0.18284867201244026 1101 82-83 -0.12478578618294023 1101 84-85 0.14202150727693663 1101 86-87 0.11827912912785621 1101 88-89 0.13555163136999582 1101 90-91 0.025269733496998015 1101 92-93 -0.01692214035444195 1101 94-95 0.03110065490957936 1101 96-97 0.06697446367414983 1101 98-99 0.22523106719308572 1101 100 -0.003733923784405846 1102 1 0.15529111293642472 1102 2 -0.020511273881290037 1102 3 0.13319504782228364 1102 4 0.15057745497284714 1102 5 0.168205683473019 1102 6 0.2309622044304689 1102 7 0.18890814314406867 1102 8 0.27346869901645476 1102 9 0.17845152522927066 1102 10-11 0.1830826629922484 1102 12-13 0.1361327154367018 1102 14-15 0.294750442688958 1102 16-17 0.1261434944392832 1102 18-19 0.13185871320062148 1102 20-21 0.026847738495597184 1102 22-23 0.09441741724486263 1102 24-25 0.2484315612444732 1102 26-27 0.38639904641010503 1102 28-29 0.3341395158056102 1102 30-31 0.14700386318744307 1102 32-33 0.09141367066273887 1102 34-35 -0.13932502555659454 1102 36-37 -0.12342638395840311 1102 38-39 -0.17577807702029702 1102 40-41 0.17823504175589733 1102 42-43 0.2746224180783372 1102 44-45 -0.06849966513642869 1102 46-47 0.19954749037121644 1102 48-49 0.5994040021155911 1102 50-51 0.3808520062051173 1102 52-53 0.23086501888058564 1102 54-55 0.22681715704160155 1102 56-57 0.13392963304584526 1102 58-59 0.21127329453880606 1102 60-61 0.023094285330358844 1102 62-63 0.09426889514261205 1102 64-65 0.007040237701666285 1102 66-67 0.10554363226919605 1102 68-69 0.15662202467356678 1102 70-71 -0.03511471405972344 1102 72-73 0.20134385112350373 1102 74-75 0.1681979634101438 1102 76-77 0.15047676261993814 1102 78-79 0.18913561268803747 1102 80-81 0.32638627908297835 1102 82-83 0.24871861639122983 1102 84-85 0.3440868837609159 1102 86-87 0.36419600295170795 1102 88-89 0.20526679992237007 1102 90-91 0.08619137000003008 1102 92-93 0.1985310111452243 1102 94-95 0.33493054594877236 1102 96-97 0.39133614850094744 1102 98-99 0.4712582957784548 1102 100 0.42085311708170536 1103 1 -0.05543978407354189 1103 2 0.10138241382635016 1103 3 0.06918618846458457 1103 4 -0.034959643587505695 1103 5 0.15277932688387352 1103 6 0.11610063189448283 1103 7 0.21698123284506465 1103 8 0.11190723722243234 1103 9 0.34247145878408247 1103 10-11 0.32870835512956376 1103 12-13 0.2765701462229657 1103 14-15 0.3453207638406681 1103 16-17 0.31957649554670553 1103 18-19 0.24394066225931255 1103 20-21 0.33198616595960573 1103 22-23 0.2077618247753179 1103 24-25 0.3586475080883247 1103 26-27 0.37152639967699486 1103 28-29 0.2870741780425945 1103 30-31 0.2836262330656254 1103 32-33 0.19356206490416383 1103 34-35 0.2579174993603459 1103 36-37 -0.15514288451211655 1103 38-39 -0.14349679241342983 1103 40-41 -0.05576274340468501 1103 42-43 -0.07995676243107397 1103 44-45 -0.07412535727374348 1103 46-47 -0.012174547281055936 1103 48-49 -0.03124383841597478 1103 50-51 -0.19495641018469456 1103 52-53 -0.34596776074288726 1103 54-55 -0.133897128672686 1103 56-57 -0.12089871689878606 1103 58-59 -0.22062039316883642 1103 60-61 0.07146637835361958 1103 62-63 -0.1011408501508555 1103 64-65 0.023679218875528818 1103 66-67 0.08298549273430211 1103 68-69 0.11283464925828213 1103 70-71 -0.011044946617865037 1103 72-73 -0.23187874023862065 1103 74-75 -0.3695207519829893 1103 76-77 -0.5618211332826064 1103 78-79 -0.5075919620627936 1103 80-81 -0.39586732003108693 1103 82-83 -0.37646410786125983 1103 84-85 -0.2681999379445088 1103 86-87 -0.4086556028068742 1103 88-89 -0.39883065301229337 1103 90-91 -0.5755694273421597 1103 92-93 -0.43016223359453676 1103 94-95 -0.31037953157060727 1103 96-97 -0.1825010608013784 1103 98-99 -0.42615587919386044 1103 100 -0.3336873037377863 1104 1 0.04122688259312213 1104 2 -0.08208163846122574 1104 3 -0.08571577231973038 1104 4 -0.14907729064633202 1104 5 -0.11238407180893972 1104 6 0.006427429280101649 1104 7 -0.2134109240176798 1104 8 -0.052210409836391136 1104 9 -0.1778553386015389 1104 10-11 -0.1124681154586682 1104 12-13 -0.11274357926722445 1104 14-15 -0.27177073942730345 1104 16-17 -0.09493330837486269 1104 18-19 -0.10710508937467722 1104 20-21 -0.06206612162209524 1104 22-23 -0.011846018361943322 1104 24-25 0.12318999174846113 1104 26-27 -0.017820005551762108 1104 28-29 0.0713879035327949 1104 30-31 -0.07009925713045817 1104 32-33 0.0694444178453395 1104 34-35 0.019616845765575874 1104 36-37 0.08596822659899317 1104 38-39 0.14147052784800707 1104 40-41 0.2729954265299597 1104 42-43 0.3166772244970275 1104 44-45 0.24339098259552827 1104 46-47 0.5217143416078329 1104 48-49 0.7921221746559226 1104 50-51 1.0416775767434103 1104 52-53 0.8280845268388148 1104 54-55 0.8577695379939811 1104 56-57 0.8732842896371622 1104 58-59 0.9885299336285485 1104 60-61 0.8039500384843308 1104 62-63 0.795623855731499 1104 64-65 0.8356073234506951 1104 66-67 0.9939005254140483 1104 68-69 0.8810699433759339 1104 70-71 1.1410138769115505 1104 72-73 1.1468794296960212 1104 74-75 1.0001524506313828 1104 76-77 0.9623945529919027 1104 78-79 0.937800194799955 1104 80-81 1.0988712420604188 1104 82-83 0.9979803365831827 1104 84-85 1.0628131339509146 1104 86-87 1.2474555083042347 1104 88-89 1.109339281628234 1104 90-91 1.0507050824617608 1104 92-93 1.1055240409152667 1104 94-95 0.9067119716973657 1104 96-97 1.0490348869110413 1104 98-99 0.9324388920479691 1104 100 0.8548747877654819 1105 1 -0.005261742530514368 1105 2 -0.009982571336848878 1105 3 0.11923564444875723 1105 4 -0.09927220540748749 1105 5 0.03350138425281557 1105 6 0.09892951418923701 1105 7 0.09932544649491604 1105 8 0.11282711852807381 1105 9 0.002145049288536427 1105 10-11 0.11258570428881853 1105 12-13 0.1414019958767767 1105 14-15 0.06430691616510131 1105 16-17 0.03392862215402204 1105 18-19 0.16899506384190488 1105 20-21 0.32953314914457366 1105 22-23 0.13857290291577584 1105 24-25 0.14803919948298017 1105 26-27 0.02920196842081424 1105 28-29 0.062049450050515986 1105 30-31 0.049016935340596035 1105 32-33 0.12048590268853587 1105 34-35 0.24874835989447064 1105 36-37 0.3410786782969808 1105 38-39 0.009272742700318304 1105 40-41 0.06154685105624935 1105 42-43 0.16172968662925769 1105 44-45 -0.015806860735665396 1105 46-47 -0.12953854332457837 1105 48-49 0.017791769892632203 1105 50-51 0.2984511071249045 1105 52-53 0.18871077535997927 1105 54-55 0.3046043550068447 1105 56-57 0.34801325144938033 1105 58-59 0.16661996291424686 1105 60-61 0.09603611129130485 1105 62-63 0.16229634074924348 1105 64-65 -0.00839298686136658 1105 66-67 0.0065166500043361 1105 68-69 -0.14930184925803758 1105 70-71 -0.22637135611341108 1105 72-73 -0.2517600458766083 1105 74-75 -0.32479275989594925 1105 76-77 -0.3441307277435399 1105 78-79 -0.20060383149899508 1105 80-81 -0.021272859101316044 1105 82-83 -0.08667182299479137 1105 84-85 0.0034271639348162353 1105 86-87 -0.056286661164939744 1105 88-89 -0.0046664591448326576 1105 90-91 -0.0576762522679779 1105 92-93 -0.030290819153389492 1105 94-95 -0.23079990743608647 1105 96-97 -0.11032005189930061 1105 98-99 -0.2451420315182915 1105 100 -0.24075950947468172 1106 1 0.049187081598098814 1106 2 0.256158533229339 1106 3 0.10003195960886302 1106 4 0.14305030666124452 1106 5 0.09163504827690616 1106 6 -0.12250633327965232 1106 7 0.023697650755508448 1106 8 0.061310222297059624 1106 9 0.06500877221692036 1106 10-11 0.15507651433354397 1106 12-13 0.11982885269063104 1106 14-15 0.1788779777710161 1106 16-17 0.1917655502730753 1106 18-19 0.3147366821598112 1106 20-21 0.2554687530242816 1106 22-23 0.3015429193026833 1106 24-25 0.2627519857002625 1106 26-27 0.3108796335078452 1106 28-29 0.1686662178435938 1106 30-31 0.0722331982397506 1106 32-33 0.17376106987928353 1106 34-35 0.29388764861407424 1106 36-37 0.06764318513963019 1106 38-39 0.11894101853184225 1106 40-41 0.19548103768984504 1106 42-43 -0.020280145515656045 1106 44-45 0.11622290143272096 1106 46-47 -0.1733934527536931 1106 48-49 0.04599496755417931 1106 50-51 0.02337692314863915 1106 52-53 0.018012338759596958 1106 54-55 0.061998393715370526 1106 56-57 -0.014580308938583642 1106 58-59 0.08236468145803144 1106 60-61 0.1308693634282463 1106 62-63 0.26793875183919624 1106 64-65 0.13176379598996135 1106 66-67 0.4759705673611734 1106 68-69 0.5838794253776882 1106 70-71 0.63649236681497 1106 72-73 0.5099123045374938 1106 74-75 0.48232004403690354 1106 76-77 0.3577559811452531 1106 78-79 0.2954677394297427 1106 80-81 0.24425705807836806 1106 82-83 0.05278962348202043 1106 84-85 0.18386473867240483 1106 86-87 0.24952847679511336 1106 88-89 0.341965366888207 1106 90-91 0.3353260950459003 1106 92-93 0.2811312987437731 1106 94-95 0.277207533106008 1106 96-97 0.3544392377060852 1106 98-99 0.27610780239817956 1106 100 0.4158151838244031 1107 1 -0.005995339629095042 1107 2 0.2308268582707953 1107 3 0.1441861884645803 1107 4 0.006151467523601184 1107 5 -0.07610956200501562 1107 6 -0.07167714588329943 1107 7 0.1419812328450618 1107 8 -0.055314984999789374 1107 9 -0.03030631899369496 1107 10-11 -0.12268053375932197 1107 12-13 0.035459035111856 1107 14-15 4.2986062886996024E-5 1107 16-17 0.05318760665781497 1107 18-19 -0.17883711551846204 1107 20-21 -0.08690272292928114 1107 22-23 0.08553960255309079 1107 24-25 -0.1441302696894482 1107 26-27 -0.15319582254522857 1107 28-29 -0.025425821957398398 1107 30-31 -0.3299848780454866 1107 32-33 -0.42810460176250587 1107 34-35 -0.27041583397299007 1107 36-37 -0.1293095511787783 1107 38-39 -0.4004412368578727 1107 40-41 -0.3724294100713479 1107 42-43 -0.26829009576441365 1107 44-45 -0.6071809128293069 1107 46-47 -0.011618991725498518 1107 48-49 -0.09096606063819479 1107 50-51 -0.18495641018469655 1107 52-53 -0.11652331629844781 1107 54-55 -0.21167490645046172 1107 56-57 -0.24673205023211864 1107 58-59 -0.0983981709466164 1107 60-61 -0.0718669549797113 1107 62-63 0.13663692762692392 1107 64-65 0.0014569966533102274 1107 66-67 0.10770771495653264 1107 68-69 0.040056871480501854 1107 70-71 0.17451060893769466 1107 72-73 0.3139545930947065 1107 74-75 0.18797924801700816 1107 76-77 0.04956775560627946 1107 78-79 -0.11759196206279299 1107 80-81 -0.07531176447552923 1107 82-83 -0.07563077452792655 1107 84-85 -0.0031999379445082354 1107 86-87 0.06967773052645754 1107 88-89 0.012280458098818059 1107 90-91 0.021375017102279514 1107 92-93 -0.20321778915009148 1107 94-95 -0.04287953157060542 1107 96-97 -0.12555661635693127 1107 98-99 -0.34476699030497215 1107 100 -0.36702063707111776 1108 1 0.06458717010435322 1108 2 0.05289184778861511 1108 3 0.09596067184283896 1108 4 0.11359381553199199 1108 5 -0.08259353924371027 1108 6 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-0.20159674632217417 2215 5 -0.3280558541952132 2215 6 -0.13539973765467295 2215 7 -0.28845113966048075 2215 8 -0.26917923727867077 2215 9 -0.22708508223587387 2215 10-11 -0.16537885846983613 2215 12-13 -0.292214037073407 2215 14-15 -0.3198048828703435 2215 16-17 -0.40819142758707017 2215 18-19 -0.36855748999493443 2215 20-21 -0.28297318363388513 2215 22-23 -0.272541205527709 2215 24-25 -0.44606424357463936 2215 26-27 -0.2105652485122178 2215 28-29 -0.36111503112221754 2215 30-31 -0.31864279871560086 2215 32-33 -0.24822655298201823 2215 34-35 -0.0781578886662615 2215 36-37 -0.03530548613812812 2215 38-39 -0.0852188914526053 2215 40-41 0.010608283941955676 2215 42-43 0.01533887688895419 2215 44-45 0.14470686741213967 2215 46-47 0.21948472840260536 2215 48-49 0.25827944318048424 2215 50-51 0.2519800273614976 2215 52-53 0.1687255134625829 2215 54-55 0.4688190575800917 2215 56-57 0.42057208871836593 2215 58-59 0.31374767778459756 2215 60-61 0.40171028079264204 2215 62-63 0.3081015740915731 2215 64-65 0.24372726026503244 2215 66-67 0.47719835304472724 2215 68-69 0.3017038288591678 2215 70-71 0.5166823261094109 2215 72-73 0.5140192641959658 2215 74-75 0.4948990558514552 2215 76-77 0.6901781276190917 2215 78-79 0.5887827607753451 2215 80-81 0.7164349711736406 2215 82-83 0.7048662690788738 2215 84-85 0.5711533510429234 2215 86-87 0.4824271762027301 2215 88-89 0.4821745206758088 2215 90-91 0.545694430750963 2215 92-93 0.4461940117861012 2215 94-95 0.40023761550995474 2215 96-97 0.6001486066043888 2215 98-99 0.44939105354819375 2215 100 0.49473102590005524 2216 1 -0.14090370159931354 2216 2 -0.06078253462725769 2216 3 -0.06620900053885492 2216 4 -0.08932390475589358 2216 5 -0.049248164525060645 2216 6 -0.16211242652476443 2216 7 -0.1464208290106086 2216 8 0.00994847433583601 2216 9 -0.14093060307158822 2216 10-11 -0.1644187926367522 2216 12-13 -0.1548903348766828 2216 14-15 -0.19799538736207722 2216 16-17 -0.3016949821165298 2216 18-19 -0.47004559203622165 2216 20-21 -0.386845449160667 2216 22-23 -0.3243687594171192 2216 24-25 -0.16996073933435696 2216 26-27 -0.20232239757386594 2216 28-29 -0.3122385367340357 2216 30-31 -0.17578957449450883 2216 32-33 -0.2180530553707527 2216 34-35 -0.2955876552788297 2216 36-37 -0.11569271269081582 2216 38-39 -0.08799851062649111 2216 40-41 -0.04098611110227779 2216 42-43 -0.01863373837609572 2216 44-45 0.09549663585339374 2216 46-47 0.09657115718629683 2216 48-49 0.16076647086238438 2216 50-51 0.14708826335483138 2216 52-53 0.26111127705780035 2216 54-55 0.20089668576030562 2216 56-57 0.19171296695001416 2216 58-59 0.11546208105796296 2216 60-61 0.07950761546702267 2216 62-63 -0.036415764240196324 2216 64-65 0.0019438236865241265 2216 66-67 -0.036292857781155874 2216 68-69 -0.1803612270303745 2216 70-71 -0.14882845177250204 2216 72-73 -0.06693028663037381 2216 74-75 -0.08525958359810915 2216 76-77 -0.1549998274406832 2216 78-79 -0.10805587958856933 2216 80-81 0.05401240505482363 2216 82-83 -0.17584555116022926 2216 84-85 -0.1966707283225162 2216 86-87 -0.21434288803024515 2216 88-89 -0.1892086598851428 2216 90-91 -0.06598179847618013 2216 92-93 -0.1419491751752915 2216 94-95 -0.06173691988675856 2216 96-97 -0.2729649783271526 2216 98-99 -0.3869634393313177 2216 100 -0.5799072350092622 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 2.0 4 2.0 5 14.0 6 37.0 7 86.0 8 142.0 9 234.0 10 407.0 11 730.0 12 1131.0 13 1632.0 14 1667.0 15 2432.0 16 3270.0 17 4397.0 18 4775.0 19 2183.0 20 963.0 21 860.0 22 826.0 23 953.0 24 1157.0 25 1602.0 26 1940.0 27 2188.0 28 1639.0 29 2059.0 30 2617.0 31 3239.0 32 4059.0 33 5209.0 34 7170.0 35 10814.0 36 17660.0 37 39139.0 38 74226.0 39 38119.0 40 118.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.15836042721381 20.806848108714977 14.69830842365786 21.336483040413356 2 16.236452542592662 22.62503816921616 38.95895292951349 22.179556358677694 3 18.330760565730735 27.084567566439986 33.242104384830405 21.342567482998874 4 11.897768803297565 19.71613570749128 38.34420840078101 30.04188708843015 5 11.06512751409059 38.58589314098098 36.11904930767337 14.229930037255055 6 26.76690349146221 39.673090000375474 17.57036950508763 15.989637003074689 7 22.14193634516623 36.166608955398225 23.22537849552981 18.466076203905732 8 23.610641768566147 33.907808608368065 22.114169138537402 20.367380484528383 9 23.96338741504971 16.009244928013885 23.339688526026393 36.68767913091002 10-11 22.547586019879223 27.012588783779556 29.207021349826345 21.232803846514877 12-13 24.84636982215195 25.63381574391216 29.675968610632502 19.843845823303393 14-15 21.665967872139376 26.86124443832565 29.65204204448505 21.82074564504993 16-17 18.178082420378978 29.966874984355314 30.67401480195913 21.18102779330658 18-19 17.87436691169722 27.027342739614014 35.298166859965455 19.800123488723308 20-21 19.87784679952774 25.352629756486262 35.1835009741384 19.5860224698476 22-23 18.965035315124386 26.030771926457763 35.589218144422794 19.41497461399505 24-25 19.79098655597159 26.1550495937588 35.34809499473294 18.705868855536668 26-27 18.978802581571053 25.754800812685914 36.23899140171632 19.027405204026717 28-29 18.674579417807863 26.058886721805607 36.12699339792864 19.13954046245789 30-31 19.169041172470475 25.873699931998047 35.83035390218566 19.12690499334582 32-33 18.534912536139075 26.026600027534535 36.09818981305721 19.340297623269183 34-35 19.39661825873283 25.26564566393685 35.72000717566615 19.61772890166417 36-37 19.139420690115518 25.855552171681985 35.86352049862536 19.141506639577134 38-39 18.97942836640954 25.735401482692875 35.763603519413934 19.52156663148365 40-41 18.99882769640257 25.58208419726407 35.60757449968502 19.811513606648337 42-43 19.705547373998222 25.41625121506556 34.71374515538238 20.16445625555384 44-45 19.593949077801742 25.709744304314995 34.24106900738009 20.455237610503172 46-47 20.54117872832177 26.314669648183763 32.99367122933346 20.15048039416101 48-49 20.45586339534166 26.073742485367063 32.257956854221334 21.21243726506994 50-51 19.724529514098933 26.13694675405404 32.32470723699306 21.813816494853963 52-53 20.112307519013427 26.431900007926608 31.861626456514212 21.594166016545753 54-55 19.777304035477826 26.722472767929776 31.42754037355183 22.072682823040562 56-57 20.417481925247916 26.355554257631447 30.654487503076776 22.57247631404386 58-59 19.854484165557636 26.42251323534933 31.448817058060317 22.27418554103271 60-61 20.02991251527958 27.2379108798952 30.726869949394864 22.005306655430353 62-63 19.080388320351773 27.78317806916174 30.61694041276768 22.519493197718806 64-65 19.087689143467433 27.809461032378106 30.401670428328863 22.701179395825598 66-67 19.098744675614 27.467782510565335 30.117772706602864 23.3157001072178 68-69 19.59728659694033 27.65218044297223 29.94860220526577 22.801930754821672 70-71 19.99737170367836 27.034322212441435 29.859114973362427 23.109191110517774 72-73 19.70517128809734 26.54960293869173 30.626182475067637 23.119043298143293 74-75 19.8171456701947 27.022223705564063 29.84722506143121 23.313405562810026 76-77 19.953358170038257 26.674287335366436 29.651145812039264 23.72120868255604 78-79 20.21785656177122 26.36890433418579 30.048101994584876 23.36513710945811 80-81 20.39265912665468 26.233734809073045 29.743136183296553 23.630469880975724 82-83 19.860116229104 26.074159675259388 30.27463610611642 23.79108798952019 84-85 19.74330305925348 26.328019724738112 29.80383731262959 24.12483990337882 86-87 19.833874638347755 26.50287757328477 29.400058823897524 24.263188964469947 88-89 19.56611527149085 26.27818686247106 29.22338805565406 24.93230981038403 90-91 19.812344072025166 25.725508356487854 29.392266806845395 25.06988076464158 92-93 19.823444869283705 26.032286393114685 29.26989530597813 24.87437343162348 94-95 19.356817001357957 25.59569626009341 29.59697286382685 25.450513874721786 96-97 18.922273351161248 25.65989011218236 29.878097113463138 25.539739423193254 98-99 19.35281332003888 25.780249396117632 29.052686911501507 25.81425037234198 100 19.50863374482163 25.4590131790287 28.84993262383239 26.182420452317277 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 201.0 1 124.0 2 128.5 3 207.5 4 131.5 5 82.0 6 150.5 7 267.5 8 418.0 9 507.0 10 363.0 11 183.0 12 161.5 13 160.5 14 159.0 15 167.0 16 176.5 17 204.5 18 241.5 19 278.0 20 332.0 21 429.0 22 588.5 23 796.5 24 1007.0 25 1270.0 26 1671.0 27 2214.0 28 3079.0 29 3858.0 30 4396.5 31 5259.5 32 6237.0 33 7125.5 34 7919.0 35 8610.0 36 9078.5 37 9278.5 38 9250.5 39 9109.0 40 9063.5 41 8802.0 42 8296.0 43 8039.5 44 7862.5 45 7578.0 46 7502.0 47 8170.0 48 9614.0 49 9369.0 50 7867.0 51 7011.0 52 6229.0 53 5489.5 54 4863.0 55 4558.5 56 4334.5 57 3893.0 58 3265.5 59 2669.0 60 2209.5 61 1740.5 62 1341.0 63 1006.0 64 754.5 65 557.0 66 396.0 67 296.5 68 216.0 69 165.5 70 130.5 71 100.0 72 98.0 73 84.5 74 50.0 75 58.5 76 64.5 77 37.5 78 20.5 79 47.5 80 46.5 81 27.0 82 35.0 83 17.5 84 1.0 85 1.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.041718989232328876 2 0.2636640119483185 3 0.0037547090309095993 4 0.0029203292462630214 5 0.0 6 0.0 7 0.0 8 8.343797846465775E-4 9 0.0 10-11 6.257848384849332E-4 12-13 0.0 14-15 2.0859494616164438E-4 16-17 4.1718989232328877E-4 18-19 4.1718989232328877E-4 20-21 0.0 22-23 0.0 24-25 6.257848384849332E-4 26-27 0.0 28-29 6.257848384849332E-4 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 2.0859494616164438E-4 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 2.0859494616164438E-4 88-89 0.001668759569293155 90-91 0.002085949461616444 92-93 2.0859494616164438E-4 94-95 2.0859494616164438E-4 96-97 0.0 98-99 0.0 100 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 239699.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 77.52401001731555 #Duplication Level Percentage of deduplicated Percentage of total 1 91.24662397915654 70.73804191406357 2 6.724352933723682 10.4259760838792 3 0.9167074748675221 2.132005183937335 4 0.3180591788838836 0.986288918795734 5 0.15650461590975664 0.6066432705772049 6 0.10356948482533321 0.4817473067452406 7 0.06062825494335887 0.32901018104929197 8 0.057726507907312 0.35801523018168807 9 0.04847537905807747 0.338220519452241 >10 0.3124990282641225 4.741472154726916 >50 0.029577685640411498 1.5558916977589536 >100 0.021511044102117453 3.616239171050687 >500 0.0016133283076588088 1.0047424950694206 >1k 0.0021511044102117453 2.6857058727125347 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1839 0.7672122119825281 No Hit CTTATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGT 1770 0.7384261094122211 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1749 0.7296651216734321 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1084 0.45223384327844507 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 925 0.38590065039904214 No Hit ATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTT 765 0.3191502676273159 RNA PCR Primer, Index 31 (95% over 22bp) GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 720 0.3003767224727679 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 497 0.20734337648467455 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 490 0.2044230472384115 No Hit ACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCT 434 0.18106041326830735 RNA PCR Primer, Index 31 (95% over 24bp) GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 356 0.14851960166709083 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 321 0.1339179554357757 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 320 0.13350076554345242 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 316 0.13183200597415926 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 305 0.12724291715860309 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 302 0.1259913474816332 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 287 0.11973349909678388 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 271 0.11305846081961127 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 253 0.10554904275779206 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 246 0.10262871351152904 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 245 0.10221152361920575 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 242 0.10095995394223589 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 4.1718989232328877E-4 0.0 14-15 0.0 0.0 0.0 0.0012515696769698664 0.0 16-17 0.0 0.0 0.0 0.001668759569293155 0.0 18-19 0.0 0.0 0.0 0.0018773545154547994 0.0 20-21 0.0 0.0 0.0 0.003754709030909599 0.0 22-23 0.0 0.0 0.0 0.006257848384849332 0.0 24-25 0.0 0.0 0.0 0.012098506877375376 0.0 26-27 0.0 0.0 0.0 0.02419701375475075 0.0 28-29 0.0 0.0 0.0 0.048185432563339854 0.0 30-31 0.0 0.0 0.0 0.11055532146567154 0.0 32-33 0.0 0.0 0.0 0.2288286559393239 0.0 34-35 0.0 0.0 0.0 0.40571717028439835 0.0 36-37 0.0 0.0 0.0 0.667503827717262 0.0 38-39 0.0 0.0 0.0 0.9561992332049778 0.0 40-41 0.0 0.0 0.0 1.2530298415929977 0.0 42-43 0.0 0.0 0.0 1.5141907141873765 0.0 44-45 0.0 0.0 0.0 1.7857813340898376 0.0 46-47 0.0 0.0 0.0 2.109312095586548 0.0 48-49 0.0 0.0 0.0 2.4226217047213376 0.0 50-51 0.0 0.0 0.0 2.734262554286835 0.0 52-53 0.0 0.0 0.0 3.017951681066671 0.0 54-55 0.0 0.0 0.0 3.3523293797637868 0.0 56-57 0.0 0.0 0.0 3.754917625855761 0.0 58-59 0.0 0.0 0.0 4.156462897216926 0.0 60-61 0.0 0.0 0.0 4.532768180092533 0.0 62-63 0.0 0.0 0.0 4.928472792961172 0.0 64-65 0.0 0.0 0.0 5.361307306246585 0.0 66-67 0.0 0.0 0.0 5.885715000896958 0.0 68-69 0.0 0.0 0.0 6.4647745714416835 0.0 70-71 0.0 0.0 0.0 7.029441090701255 0.0 72-73 0.0 0.0 0.0 7.605580331999716 0.0 74-75 0.0 0.0 0.0 8.205082207268283 0.0 76-77 0.0 0.0 0.0 8.855064059507967 0.0 78-79 0.0 0.0 0.0 9.624570815898272 0.0 80-81 0.0 0.0 0.0 10.395329141965549 0.0 82-83 0.0 0.0 0.0 11.163167138786562 0.0 84-85 0.0 0.0 0.0 11.935802819369293 0.0 86-87 0.0 0.0 0.0 12.76267318595405 0.0 88 0.0 0.0 0.0 13.442692710441012 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAACTCG 20 0.0021616272 70.4939 5 CTATACA 55 5.163778E-5 42.87565 1 TAATAGT 85 1.750055E-5 33.1736 4 CCTTAGA 70 0.008692112 26.939125 2 CTCAGCG 35 0.008834706 26.854816 42-43 TCTATAC 70 0.008826052 26.854816 3 TATACCA 95 0.0013022328 24.734701 5 CATGGGC 295 0.0 23.896236 4 GGTATCA 2575 0.0 23.810553 1 ATGGGCA 185 2.7978604E-7 22.862886 5 TCTAGCA 105 0.0022892284 22.449272 2 GAGTACT 2250 0.0 21.826998 12-13 TATATAC 130 3.1514087E-4 21.69043 3 GTATCAA 3375 0.0 21.380655 1 AGTACTT 2285 0.0 21.081326 12-13 GTACATG 1040 0.0 20.40716 1 AGAGTAC 2825 0.0 20.21241 10-11 TACATGG 1020 0.0 19.874207 2 CATGGGG 355 3.6379788E-12 19.857437 4 TAGGTGG 120 0.005055199 19.581638 7 >>END_MODULE