FastQCFastQC Report
Wed 25 May 2016
SRR1033085_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033085_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3973594
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA47000.035.3071331
TCAACGC46800.033.2448164
ATCAACG47650.032.7504273
CAACGCA47650.032.553145
GGTATCA29900.031.6016981
AACGCAG49350.031.3294016
TATCAAC54350.029.6644542
GTACATG107700.028.3715611
TACATGG108200.026.5867942
ACGCAGA59050.025.7854027
ACATGGG110400.024.7371043
CGCAGAG62250.024.4083718
CATGGGG82900.021.3761084
GAGTACA75050.019.5429211
GCAGAGT78150.018.663399
AGTACAT74250.017.6623942
TATACTG23450.017.639315
AGAGTAC63150.016.09831810-11
TACACTG29500.015.7744775
GTATAGG16150.015.7183451
Produced by FastQC (version 0.11.5)