FastQCFastQC Report
Wed 25 May 2016
SRR1033085_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033085_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3973594
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56970.1433714667376687No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47760.12019345710709246No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA52400.051.9958081
GTATCAA92500.043.214141
TCAACGC107300.036.705394
ATCAACG107500.036.6812863
CAACGCA109250.036.0493355
TATCAAC113800.034.7772372
AACGCAG114950.034.4253046
ACGCAGA130250.030.1649917
CGCAGAG134450.029.1531438
GTACATG104900.026.8718431
TACATGG105100.025.641972
GCAGAGT151350.025.494179
TATACCG2251.1641532E-1025.0653295
ACATGGG107450.024.1010653
GAGTACT97400.022.53370312-13
CAGAGTA151700.021.05154410-11
AGAGTAC137650.020.92976610-11
GTACTTT111550.019.54881114-15
CATGGGG83200.019.4321444
AGTACTT103700.019.14796412-13
Produced by FastQC (version 0.11.5)