##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1033085_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3973594 Sequences flagged as poor quality 0 Sequence length 100 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.72774370003579 34.0 31.0 34.0 31.0 34.0 2 32.72260452376363 34.0 31.0 34.0 31.0 34.0 3 32.827245561574735 34.0 31.0 34.0 31.0 34.0 4 36.31258427509202 37.0 37.0 37.0 35.0 37.0 5 36.29398901850566 37.0 37.0 37.0 35.0 37.0 6 36.3619353159885 37.0 37.0 37.0 35.0 37.0 7 36.310274275630576 37.0 37.0 37.0 35.0 37.0 8 36.3165975185185 37.0 37.0 37.0 35.0 37.0 9 38.12212017634413 39.0 39.0 39.0 37.0 39.0 10-11 38.082436580083424 39.0 39.0 39.0 36.0 39.0 12-13 38.058143962367566 39.0 39.0 39.0 35.0 39.0 14-15 39.56351139547725 41.0 39.5 41.0 37.0 41.0 16-17 39.563189268959036 41.0 40.0 41.0 37.0 41.0 18-19 39.57342383242978 41.0 40.0 41.0 37.0 41.0 20-21 39.56335448463028 41.0 40.0 41.0 37.0 41.0 22-23 39.495975180151774 41.0 39.0 41.0 36.5 41.0 24-25 39.445679905898785 41.0 39.0 41.0 36.0 41.0 26-27 39.340604878102795 41.0 39.0 41.0 36.0 41.0 28-29 39.290297775766724 41.0 39.0 41.0 36.0 41.0 30-31 39.294103272755095 41.0 39.0 41.0 36.0 41.0 32-33 39.2633464817996 41.0 39.0 41.0 35.5 41.0 34-35 39.18933237769133 41.0 39.0 41.0 35.0 41.0 36-37 39.10940045711766 41.0 39.0 41.0 35.0 41.0 38-39 39.01972886510298 41.0 39.0 41.0 35.0 41.0 40-41 38.92096223217571 41.0 39.0 41.0 35.0 41.0 42-43 38.80674397032007 41.0 39.0 41.0 35.0 41.0 44-45 38.64920107590257 41.0 38.5 41.0 35.0 41.0 46-47 38.46118098628094 41.0 38.0 41.0 35.0 41.0 48-49 38.2655593399829 40.0 38.0 41.0 34.0 41.0 50-51 38.12597285983419 40.0 38.0 41.0 34.0 41.0 52-53 37.984110983658624 40.0 38.0 41.0 34.0 41.0 54-55 37.82862139413337 40.0 37.0 41.0 33.5 41.0 56-57 37.667717562488775 40.0 37.0 41.0 33.0 41.0 58-59 37.47811552966911 40.0 36.5 41.0 33.0 41.0 60-61 37.276007815594646 40.0 36.0 41.0 33.0 41.0 62-63 37.035730122403045 39.0 35.5 41.0 33.0 41.0 64-65 36.76421723004414 39.0 35.0 41.0 32.0 41.0 66-67 36.47803474637821 38.5 35.0 41.0 32.0 41.0 68-69 36.16476356165225 37.5 35.0 40.0 32.0 41.0 70-71 35.83315809315194 37.0 35.0 40.0 31.0 41.0 72-73 35.488187268251366 36.5 35.0 39.0 31.0 41.0 74-75 35.142631079068465 36.0 35.0 39.0 31.0 41.0 76-77 34.79166027027422 35.5 35.0 38.5 31.0 40.0 78-79 34.436996960434314 35.0 34.5 37.0 30.5 39.0 80-81 34.1150980699085 35.0 34.0 37.0 30.0 39.0 82-83 33.82896969343118 35.0 34.0 36.5 30.0 39.0 84-85 33.617569510121065 35.0 34.0 36.0 30.0 37.0 86-87 33.418765102826306 35.0 34.0 36.0 30.0 37.0 88-89 33.222758917997155 35.0 34.0 35.5 30.0 37.0 90-91 33.04704607466188 35.0 34.0 35.0 30.0 36.0 92-93 32.88009960252607 35.0 34.0 35.0 30.0 36.0 94-95 32.73479839661526 35.0 34.0 35.0 29.5 36.0 96-97 32.59720331266858 35.0 34.0 35.0 29.0 36.0 98-99 32.476683450800465 35.0 34.0 35.0 29.0 35.0 100 32.37542662889062 35.0 34.0 35.0 29.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0893355860246885 1101 2 0.05298628804312955 1101 3 0.12260212534727799 1101 4 0.11404017700494506 1101 5 0.11772387905607218 1101 6 0.09842776051069535 1101 7 0.14306985318761178 1101 8 0.14438097891620316 1101 9 0.22389619375557146 1101 10-11 0.19379417525124865 1101 12-13 0.235563758118154 1101 14-15 0.2522239278397578 1101 16-17 0.28227538938600105 1101 18-19 0.2511414474784175 1101 20-21 0.24642363797122613 1101 22-23 0.23732893033955094 1101 24-25 0.27371520466346766 1101 26-27 0.2900630171109526 1101 28-29 0.2950305601000309 1101 30-31 0.2918913514109178 1101 32-33 0.28164677338430266 1101 34-35 0.30023301493312715 1101 36-37 0.3100713818530636 1101 38-39 0.317979433221808 1101 40-41 0.41911168984069036 1101 42-43 0.3900607878449378 1101 44-45 0.38690677126140827 1101 46-47 0.3763793853438244 1101 48-49 0.44785395558508867 1101 50-51 0.43332317449305435 1101 52-53 0.46708115128827643 1101 54-55 0.4428870576630075 1101 56-57 0.37484238397379244 1101 58-59 0.36687738270909875 1101 60-61 0.36966862015601976 1101 62-63 0.355473893931439 1101 64-65 0.3332929790210244 1101 66-67 0.3355350753817845 1101 68-69 0.29060839031412655 1101 70-71 0.3142081955694991 1101 72-73 0.3276811110116711 1101 74-75 0.3296654210908727 1101 76-77 0.31539503669380053 1101 78-79 0.29249768763722983 1101 80-81 0.3163831183631416 1101 82-83 0.2944417234535237 1101 84-85 0.30162656293207846 1101 86-87 0.33019763723241624 1101 88-89 0.3211832518627702 1101 90-91 0.3344930736557643 1101 92-93 0.36339194029905997 1101 94-95 0.30775395139629325 1101 96-97 0.3643570057909784 1101 98-99 0.39074012522581114 1101 100 0.4157404722913256 1102 1 0.17365181744131064 1102 2 0.08757788390331456 1102 3 0.11938454706707802 1102 4 0.0942993510391048 1102 5 0.10119393848464853 1102 6 0.11334790269734185 1102 7 0.13488222291537255 1102 8 0.12464493358305617 1102 9 0.1665483612944314 1102 10-11 0.1863758550168484 1102 12-13 0.192651949105489 1102 14-15 0.3019807529897065 1102 16-17 0.2918467472344233 1102 18-19 0.2930768388210083 1102 20-21 0.2923905899885355 1102 22-23 0.27237144609970443 1102 24-25 0.3051770796488569 1102 26-27 0.2983756874873933 1102 28-29 0.3071339003812099 1102 30-31 0.27948513148906073 1102 32-33 0.25734793284708957 1102 34-35 0.24502478119632087 1102 36-37 0.24757332066521798 1102 38-39 0.24905120857881258 1102 40-41 0.30857942481941336 1102 42-43 0.2811123464374319 1102 44-45 0.32222449211069915 1102 46-47 0.27938332246043984 1102 48-49 0.2982653024699218 1102 50-51 0.3196950248528836 1102 52-53 0.31693199554801765 1102 54-55 0.2813807652880058 1102 56-57 0.29964149116408123 1102 58-59 0.291496950582399 1102 60-61 0.32284343833262596 1102 62-63 0.2797422977737938 1102 64-65 0.3435626279526005 1102 66-67 0.33638108801540056 1102 68-69 0.3319338791603741 1102 70-71 0.35601578466455663 1102 72-73 0.336100442615475 1102 74-75 0.3735796756627465 1102 76-77 0.31806889768149915 1102 78-79 0.3355038344685255 1102 80-81 0.32276303205679113 1102 82-83 0.3455779736102258 1102 84-85 0.41204409276853937 1102 86-87 0.33742032335307215 1102 88-89 0.3540998202608705 1102 90-91 0.35660652763102263 1102 92-93 0.3620085314317265 1102 94-95 0.41781181829922787 1102 96-97 0.3901419856804509 1102 98-99 0.3700569834364913 1102 100 0.3884294613256145 1103 1 0.049168735478147596 1103 2 0.013172415574828733 1103 3 0.09265350268514538 1103 4 0.06371789449584497 1103 5 0.1188858656967895 1103 6 0.11937486558725396 1103 7 0.11707779294042808 1103 8 0.12328458937768971 1103 9 0.17647678115638143 1103 10-11 0.16225442553755443 1103 12-13 0.1616505469045464 1103 14-15 0.23264250302020884 1103 16-17 0.23705907578128915 1103 18-19 0.2467819008838461 1103 20-21 0.2467970587953161 1103 22-23 0.2551802174358002 1103 24-25 0.18552855379746802 1103 26-27 0.20740020584132424 1103 28-29 0.24208872317149854 1103 30-31 0.18787179388838382 1103 32-33 0.20946621592425174 1103 34-35 0.20196945321553983 1103 36-37 0.2041984011246072 1103 38-39 0.1814451642606869 1103 40-41 0.16917951782305352 1103 42-43 0.1895168745204998 1103 44-45 0.18334702875193898 1103 46-47 0.17791093489557142 1103 48-49 0.20267802426600667 1103 50-51 0.23385842848002625 1103 52-53 0.18637677603746283 1103 54-55 0.22792985533769183 1103 56-57 0.1926815312240393 1103 58-59 0.2528112115326593 1103 60-61 0.2694307609455251 1103 62-63 0.16918051390058508 1103 64-65 0.2297560275523054 1103 66-67 0.23219117681805557 1103 68-69 0.2093022435264018 1103 70-71 0.18875934625497592 1103 72-73 0.20260851305526728 1103 74-75 0.17197007964669808 1103 76-77 0.1963533632653025 1103 78-79 0.18772012358224544 1103 80-81 0.18244140891348337 1103 82-83 0.231187726844297 1103 84-85 0.192724805946213 1103 86-87 0.21633835712737692 1103 88-89 0.20922147791046797 1103 90-91 0.16915691523978893 1103 92-93 0.23116113142469175 1103 94-95 0.2093266904600526 1103 96-97 0.2805704200901715 1103 98-99 0.3149331819048413 1103 100 0.32102723893664376 1104 1 0.10962493438113796 1104 2 0.0882570331656467 1104 3 0.13945506082070835 1104 4 0.04435942836244067 1104 5 0.08500867169774295 1104 6 0.08309294621535912 1104 7 0.11721348623333228 1104 8 0.121441303119866 1104 9 0.10599979488493716 1104 10-11 0.14191180971172201 1104 12-13 0.15499962780160814 1104 14-15 0.18860978600385891 1104 16-17 0.22404868599255678 1104 18-19 0.23956295277630346 1104 20-21 0.1987851035020327 1104 22-23 0.1998361172616896 1104 24-25 0.2148421681035657 1104 26-27 0.19205782293400375 1104 28-29 0.19281592397955194 1104 30-31 0.1645436009935466 1104 32-33 0.20532171100756358 1104 34-35 0.2154761136299257 1104 36-37 0.2342842660873785 1104 38-39 0.15660261149328392 1104 40-41 0.1684471148977309 1104 42-43 0.15080163030422966 1104 44-45 0.26655053639109383 1104 46-47 0.2680875520520445 1104 48-49 0.21541139522338426 1104 50-51 0.1997543702279927 1104 52-53 0.18921731061060854 1104 54-55 0.24067802919975634 1104 56-57 0.2021832430533692 1104 58-59 0.19878929317034988 1104 60-61 0.18560553130978974 1104 62-63 0.17466093227092472 1104 64-65 0.19217723089536065 1104 66-67 0.18008069975387286 1104 68-69 0.18042758728409325 1104 70-71 0.13394910829341455 1104 72-73 0.18432645506846512 1104 74-75 0.11657316296838616 1104 76-77 0.13749505027379172 1104 78-79 0.05807423925233479 1104 80-81 0.11928619575083843 1104 82-83 0.18070943233369263 1104 84-85 0.1467439487638984 1104 86-87 0.18322901511486833 1104 88-89 0.1403799853686536 1104 90-91 0.17084415421076926 1104 92-93 0.1076169654490613 1104 94-95 0.1051140791968237 1104 96-97 0.17437993300156052 1104 98-99 0.1559866358104287 1104 100 0.18244673036922165 1105 1 0.08580241058076155 1105 2 0.10179878370917095 1105 3 0.1091672573929614 1105 4 0.054082743134472366 1105 5 0.0880736094172434 1105 6 0.09422848914895354 1105 7 0.0897283053380562 1105 8 0.07641785889358488 1105 9 0.0937290496868215 1105 10-11 0.09625526298121656 1105 12-13 0.122452336740146 1105 14-15 0.1837918736239459 1105 16-17 0.21704933016681593 1105 18-19 0.15664510667201625 1105 20-21 0.11339314824736846 1105 22-23 0.20424863344346988 1105 24-25 0.1871364039828336 1105 26-27 0.19957925411575417 1105 28-29 0.18901336028992688 1105 30-31 0.20691718179715934 1105 32-33 0.20898491007599063 1105 34-35 0.19394977681351122 1105 36-37 0.22714190667530687 1105 38-39 0.22253175542287096 1105 40-41 0.1588361434953498 1105 42-43 0.2075480393424911 1105 44-45 0.21057264581400403 1105 46-47 0.24261378976472514 1105 48-49 0.20645992817847514 1105 50-51 0.22879818956189268 1105 52-53 0.24876998114644522 1105 54-55 0.30649041654668707 1105 56-57 0.28813103087227887 1105 58-59 0.2848773747079463 1105 60-61 0.253861677289585 1105 62-63 0.2505369163399962 1105 64-65 0.25500450548082654 1105 66-67 0.2990654904282124 1105 68-69 0.2973943008185671 1105 70-71 0.24483387233362208 1105 72-73 0.2109585696323819 1105 74-75 0.21542603728627086 1105 76-77 0.23022517223331818 1105 78-79 0.20762246560525455 1105 80-81 0.19412871372887963 1105 82-83 0.21786396292267085 1105 84-85 0.19156771445790355 1105 86-87 0.14572127263591028 1105 88-89 0.14520711129852515 1105 90-91 0.18286017185123882 1105 92-93 0.24026227629411068 1105 94-95 0.17569080096929923 1105 96-97 0.20500399229037214 1105 98-99 0.18379885901681092 1105 100 0.20416453869022178 1106 1 0.05809877283802223 1106 2 0.05808529726361655 1106 3 0.06117248609829318 1106 4 0.04256936701722225 1106 5 0.021404347023739945 1106 6 0.05310266495614968 1106 7 0.05380645771950299 1106 8 0.052252632756243145 1106 9 0.07718711661139821 1106 10-11 0.10147127699100622 1106 12-13 0.06313652079818155 1106 14-15 0.11558280031031387 1106 16-17 0.15275069952169673 1106 18-19 0.18236869029681912 1106 20-21 0.13437900530089308 1106 22-23 0.11118210714419519 1106 24-25 0.10864236741756628 1106 26-27 0.14816992305239296 1106 28-29 0.12661869528135128 1106 30-31 0.12387222182683644 1106 32-33 0.13865273035499825 1106 34-35 0.13478708894034952 1106 36-37 0.12385931286653573 1106 38-39 0.10744968091798768 1106 40-41 0.16022538244408935 1106 42-43 0.1437364641444816 1106 44-45 0.10326057247422682 1106 46-47 0.11894043404870303 1106 48-49 0.1750680499105357 1106 50-51 0.16923972269723464 1106 52-53 0.164900144760459 1106 54-55 0.1920775640132959 1106 56-57 0.1638465300959382 1106 58-59 0.14663004315476513 1106 60-61 0.17095804572903717 1106 62-63 0.20299374207122156 1106 64-65 0.21600850488162848 1106 66-67 0.261582550892399 1106 68-69 0.23984899845007845 1106 70-71 0.2324265457261916 1106 72-73 0.19478221793239214 1106 74-75 0.20868222006982506 1106 76-77 0.2617241390944116 1106 78-79 0.26919870388584854 1106 80-81 0.2561500993476784 1106 82-83 0.27219116835919266 1106 84-85 0.24273825846675834 1106 86-87 0.15114129888643646 1106 88-89 0.22855259104829173 1106 90-91 0.155249445277569 1106 92-93 0.20715632665041994 1106 94-95 0.18410904032938902 1106 96-97 0.20400863012108772 1106 98-99 0.21688284405590963 1106 100 0.19664491618419788 1107 1 -0.016444845474445913 1107 2 -0.02103384723604762 1107 3 0.013169711892103919 1107 4 0.038860217315921375 1107 5 0.0459025354580902 1107 6 0.03265066300158992 1107 7 0.05993971644350182 1107 8 -0.0016734206877586644 1107 9 0.04096773815067678 1107 10-11 0.07732262277374247 1107 12-13 0.07932667101514568 1107 14-15 0.09177073251340317 1107 16-17 0.08456889669299983 1107 18-19 0.10285857863051717 1107 20-21 0.10829485250337711 1107 22-23 0.15053620348931673 1107 24-25 0.11937659756409147 1107 26-27 0.09146493466037242 1107 28-29 0.11757527850415528 1107 30-31 0.1145631781848735 1107 32-33 0.0758109648159504 1107 34-35 0.1015100972877363 1107 36-37 0.05163537678136265 1107 38-39 0.07853485828027829 1107 40-41 0.07320422574422736 1107 42-43 0.07385424968843068 1107 44-45 0.06680672630305651 1107 46-47 0.044940932820345836 1107 48-49 0.09259142912785734 1107 50-51 0.07795879716621101 1107 52-53 0.06373895257237194 1107 54-55 0.016459503750866133 1107 56-57 -0.003941631564622128 1107 58-59 0.038413821704658346 1107 60-61 0.024717973311439323 1107 62-63 0.06625960424811694 1107 64-65 0.054008233540848494 1107 66-67 0.08074126498478051 1107 68-69 0.09301498807833042 1107 70-71 0.041853160991983884 1107 72-73 0.10262137702365948 1107 74-75 0.09534950785543828 1107 76-77 0.06677636938054832 1107 78-79 0.035373935989177596 1107 80-81 0.07302059998831112 1107 82-83 0.07571372905058382 1107 84-85 0.06902279249244714 1107 86-87 0.09599393044167925 1107 88-89 0.06847912074602647 1107 90-91 0.07378894506477707 1107 92-93 0.11569933923117048 1107 94-95 0.03302477494234779 1107 96-97 0.06819229405482474 1107 98-99 0.09911342855323113 1107 100 0.07304102644087607 1108 1 -0.03721024104635262 1108 2 0.003624514149358049 1108 3 0.05147420963967164 1108 4 0.019700420800340623 1108 5 0.0112088834561348 1108 6 0.048035021268738376 1108 7 0.028462869373825583 1108 8 0.015088436118382731 1108 9 0.01880829313955701 1108 10-11 0.015925144548688763 1108 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48-49 -0.282770937640791 2214 50-51 -0.2182231822522951 2214 52-53 -0.19675282017745843 2214 54-55 -0.16184385047542804 2214 56-57 -0.17417868979379136 2214 58-59 -0.2184212833559087 2214 60-61 -0.278409826528879 2214 62-63 -0.2485094332588318 2214 64-65 -0.2661010464258311 2214 66-67 -0.26262316621900084 2214 68-69 -0.2971629375232041 2214 70-71 -0.3040745166990817 2214 72-73 -0.23369654505036408 2214 74-75 -0.30316531611170916 2214 76-77 -0.2882052003406983 2214 78-79 -0.23534122356008425 2214 80-81 -0.24898452662193193 2214 82-83 -0.32943493983203354 2214 84-85 -0.2564024783327383 2214 86-87 -0.26929517960429905 2214 88-89 -0.27980383539750875 2214 90-91 -0.2780749335590045 2214 92-93 -0.2911536144449869 2214 94-95 -0.2333395793317976 2214 96-97 -0.2253729282108594 2214 98-99 -0.2924442349058438 2214 100 -0.2703286936759426 2215 1 -0.20643311819696208 2215 2 -0.1749968503367043 2215 3 -0.2118323298287379 2215 4 -0.12481614653066231 2215 5 -0.1600600464516191 2215 6 -0.18100936707823223 2215 7 -0.21189713033199808 2215 8 -0.18753399914168511 2215 9 -0.2669022927825395 2215 10-11 -0.24294868292738414 2215 12-13 -0.2702288302984428 2215 14-15 -0.4131479106460958 2215 16-17 -0.3619733687637421 2215 18-19 -0.39048832488188623 2215 20-21 -0.38981181846390456 2215 22-23 -0.42927544432163955 2215 24-25 -0.4515645531589527 2215 26-27 -0.44925388413300027 2215 28-29 -0.4600500762735109 2215 30-31 -0.47465835953832425 2215 32-33 -0.4364927088133683 2215 34-35 -0.42972803664364534 2215 36-37 -0.4564911309950688 2215 38-39 -0.4538970170655503 2215 40-41 -0.42523597982264505 2215 42-43 -0.4543895744501185 2215 44-45 -0.4486658242513286 2215 46-47 -0.43055730441751905 2215 48-49 -0.4302449891156783 2215 50-51 -0.40633703166692925 2215 52-53 -0.38365491694285225 2215 54-55 -0.43651663702050314 2215 56-57 -0.4014937115046422 2215 58-59 -0.4194671289939791 2215 60-61 -0.43474762805104916 2215 62-63 -0.35389071228743774 2215 64-65 -0.3933434365868038 2215 66-67 -0.4167671129528827 2215 68-69 -0.329659755697179 2215 70-71 -0.3449219707563671 2215 72-73 -0.3708659831184633 2215 74-75 -0.37731768843514857 2215 76-77 -0.33519088600523617 2215 78-79 -0.3969851600377723 2215 80-81 -0.3458102028346772 2215 82-83 -0.393750695454834 2215 84-85 -0.41205956272660416 2215 86-87 -0.3629852366646844 2215 88-89 -0.3477716122088097 2215 90-91 -0.37514520783212646 2215 92-93 -0.3482642190340357 2215 94-95 -0.37413105515711464 2215 96-97 -0.4221289741612253 2215 98-99 -0.46333984127416983 2215 100 -0.4561102737911895 2216 1 -0.19769838507258441 2216 2 -0.16931832567433247 2216 3 -0.14970297531355214 2216 4 -0.13441446254586964 2216 5 -0.1673282071462978 2216 6 -0.14767709828129938 2216 7 -0.14291924394734679 2216 8 -0.15876954978763536 2216 9 -0.1716267575595296 2216 10-11 -0.22278351012226238 2216 12-13 -0.27398388264627016 2216 14-15 -0.3363510719395961 2216 16-17 -0.315995743525292 2216 18-19 -0.32466298043970454 2216 20-21 -0.32658489804467905 2216 22-23 -0.3056548674312367 2216 24-25 -0.341247459460547 2216 26-27 -0.308191630738186 2216 28-29 -0.3459866559858611 2216 30-31 -0.32675705558934 2216 32-33 -0.34836387393691837 2216 34-35 -0.3444163041729311 2216 36-37 -0.33839641024118094 2216 38-39 -0.36739356895350994 2216 40-41 -0.37361289934902686 2216 42-43 -0.3831494314936279 2216 44-45 -0.41710275598860846 2216 46-47 -0.3697612587138579 2216 48-49 -0.39019357292874446 2216 50-51 -0.3693863280750094 2216 52-53 -0.35656847611380726 2216 54-55 -0.3829059448389671 2216 56-57 -0.43999807004409774 2216 58-59 -0.36846565976788526 2216 60-61 -0.43234589755344643 2216 62-63 -0.4409787032977164 2216 64-65 -0.4688421459393055 2216 66-67 -0.4618232999275875 2216 68-69 -0.4475646935053703 2216 70-71 -0.4482394212433931 2216 72-73 -0.43954623544418325 2216 74-75 -0.5350753033733326 2216 76-77 -0.48255169048368884 2216 78-79 -0.5333034939917383 2216 80-81 -0.5043004405720737 2216 82-83 -0.4948940315100785 2216 84-85 -0.5421650459988285 2216 86-87 -0.5594858739214743 2216 88-89 -0.6062391316459284 2216 90-91 -0.5870313477210871 2216 92-93 -0.5525377933662483 2216 94-95 -0.5038310071866334 2216 96-97 -0.66149564220202 2216 98-99 -0.6906924297183963 2216 100 -0.7096607868022176 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 29.0 3 8.0 4 13.0 5 36.0 6 62.0 7 196.0 8 409.0 9 835.0 10 1503.0 11 2500.0 12 3685.0 13 5796.0 14 6889.0 15 8931.0 16 11319.0 17 16120.0 18 18858.0 19 9968.0 20 7083.0 21 6661.0 22 7240.0 23 7727.0 24 9051.0 25 11044.0 26 14315.0 27 18338.0 28 24205.0 29 31501.0 30 40099.0 31 51589.0 32 66696.0 33 87610.0 34 122702.0 35 188255.0 36 352201.0 37 799156.0 38 1396331.0 39 642359.0 40 2274.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.92583631470156 19.454944623340527 13.420386768012204 24.198832293945706 2 15.67983172565712 20.069308726557473 39.84764895485699 24.403210592928414 3 18.30962875494464 25.147389174363923 30.067894905591903 26.47508716509953 4 12.335380488795593 16.67555475916457 38.48929943291537 32.499765319124464 5 12.787652856376727 37.76813017369965 35.7974402447363 13.646776725187317 6 31.339023738992633 38.029977433499035 17.341199605696513 13.289799221811819 7 27.43843482751383 32.45797129752058 22.372416507574755 17.73117736739083 8 25.548805700044518 36.00810653760095 20.03526791814843 18.407819844206095 9 25.46566156482016 15.931245114624193 20.775524625817333 37.82756869473832 10-11 24.200085338994747 26.31849816455729 28.8213768771087 20.66003961933926 12-13 25.158810890090937 23.629464912620666 28.96467278740606 22.24705140988234 14-15 22.172309244869083 25.413628633607075 26.31850477497321 26.095557346550624 16-17 21.575187676634982 28.906895276060183 26.855727440425408 22.66218960687942 18-19 21.484976238004496 27.591484437679746 29.316808654782143 21.606730669533608 20-21 22.525288189986195 26.926756986244694 28.987611718761404 21.56034310500771 22-23 22.08243107328858 26.69634850027802 29.08854507131026 22.132675355123137 24-25 21.985804625991683 26.885518936728587 29.07021634066079 22.05846009661894 26-27 21.796449681805168 26.83692003762343 29.091217948516295 22.2754123320551 28-29 21.831653971786423 26.861635095877563 29.204822297052523 22.101888635283494 30-31 22.09314923287912 27.284900825864966 28.690480639492005 21.931469301763908 32-33 21.810179902627194 26.95164629300326 29.213855265535432 22.024318538834116 34-35 21.909485468319108 26.805280056291608 29.14154037881072 22.143694096578564 36-37 22.192239569518176 27.14492220392924 28.55465354537983 22.108184681172762 38-39 22.08623981211971 27.33537950782088 28.634379858636787 21.94400082142262 40-41 22.0191579809905 27.031247797962145 28.781377151957223 22.168217069090133 42-43 21.971759067483994 27.0375131430136 28.659545992871944 22.331181796630457 44-45 22.129764641279408 27.170604243916213 28.484742024474567 22.214889090329812 46-47 22.216348726115452 27.349585790596624 28.22951967412876 22.204545809159164 48-49 22.25306611596454 27.31368630011018 28.025019667333908 22.40822791659138 50-51 22.307374633644 27.31357305250612 27.950792154407324 22.42826015944256 52-53 22.32477701546761 27.296296501353684 27.873821532849103 22.505104950329603 54-55 22.380570335066942 27.608469813473647 27.713261596428822 22.297698255030586 56-57 22.132067342561925 27.3504288560935 27.926154508991104 22.59134929235347 58-59 22.09713674824353 27.02715727877584 28.202239584617857 22.673466388362776 60-61 22.25545689871688 27.398886247563286 27.86138946253694 22.484267391182893 62-63 22.110034392039047 27.352265983892664 27.91719536520339 22.620504258864894 64-65 22.176045162137854 27.162513331759612 27.97207012090314 22.689371385199394 66-67 22.038008915858036 27.21872189257383 27.935465978658115 22.807803212910024 68-69 22.387772543765298 27.28791050492981 27.843655925560363 22.48066102574453 70-71 22.296490280587296 27.14849579499063 27.847900414586896 22.707113509835178 72-73 22.25403933860608 27.302575575787813 27.834300450916082 22.609084634690024 74-75 22.2746559156833 27.392005717751456 27.661875029884815 22.671463336680432 76-77 22.282548242221022 27.336046410378113 27.609791035520992 22.771614311879876 78-79 22.419502344728727 27.41438858625214 27.62807423204283 22.538034836976298 80-81 22.349238497944178 27.227970446905246 27.74697163323681 22.675819421913765 82-83 22.197787291699804 27.352065838666878 27.791504512793463 22.658642356839856 84-85 22.220287226123254 27.255250536416153 27.62008398442317 22.90437825303743 86-87 22.32503487732255 27.370459389106095 27.591054443352647 22.713451290218707 88-89 22.099591631702708 27.217766518732304 27.657354171571562 23.025287677993425 90-91 22.03858316167505 27.23238609838442 27.704879457505292 23.024151282435238 92-93 22.15974401818418 27.312838966836694 27.585176378834763 22.942240636144355 94-95 21.967782327429187 27.104744367431305 27.725572341911146 23.201900963228354 96-97 22.02377701747918 27.12719118881313 27.687176693086972 23.161855100620723 98-99 21.941053304442583 27.242808140749297 27.607314795940198 23.208823758867926 100 22.00081336945848 27.046698782009436 27.57184050509438 23.380647343437705 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 35.0 1 34.5 2 106.5 3 193.5 4 137.5 5 91.0 6 259.5 7 617.5 8 1051.5 9 1288.0 10 1053.0 11 849.0 12 966.0 13 1146.0 14 1405.0 15 1649.5 16 1904.0 17 2254.0 18 2598.5 19 2970.5 20 3596.0 21 4565.0 22 5904.0 23 8125.5 24 11128.0 25 14936.0 26 20306.5 27 26999.0 28 35851.0 29 46337.0 30 56245.0 31 66192.0 32 76733.5 33 88014.5 34 98802.0 35 107528.0 36 114787.5 37 122126.0 38 129018.5 39 133156.0 40 137325.0 41 141241.5 42 142852.0 43 144271.5 44 142626.5 45 140220.5 46 139051.0 47 134974.5 48 131203.0 49 129104.5 50 126779.5 51 123600.5 52 117379.0 53 113149.5 54 110440.0 55 105081.0 56 97691.5 57 90213.0 58 81987.0 59 73800.0 60 65897.0 61 56284.5 62 48326.5 63 40711.5 64 32861.5 65 26684.0 66 21525.5 67 16608.0 68 12985.0 69 10454.5 70 7898.0 71 5663.0 72 4341.5 73 3283.5 74 2361.5 75 1533.0 76 882.5 77 558.0 78 346.5 79 200.5 80 130.5 81 67.5 82 24.0 83 13.5 84 6.5 85 5.0 86 4.0 87 0.5 88 0.5 89 0.5 90 0.0 91 0.0 92 0.0 93 0.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04288309273670133 2 0.24136839344935593 3 0.0025166134235153365 4 0.0028437731685723305 5 2.76827476586687E-4 6 1.2583067117576683E-4 7 0.0 8 2.76827476586687E-4 9 0.0 10-11 7.424009599370242E-4 12-13 0.0 14-15 2.8941054370426367E-4 16-17 1.0066453694061347E-4 18-19 5.53654953173374E-4 20-21 0.0 22-23 1.8874600676365024E-4 24-25 0.0011576421748170547 26-27 1.6357987252849687E-4 28-29 2.642444094691103E-4 30-31 6.291533558788341E-5 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 5.0332268470306735E-5 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 3.019936108218404E-4 70-71 0.0 72-73 4.1524121488003055E-4 74-75 1.0066453694061347E-4 76-77 0.0 78-79 0.0 80-81 0.0 82-83 5.0332268470306735E-5 84-85 0.0 86-87 5.914041545261041E-4 88-89 0.0024536980879274533 90-91 0.002491447289280183 92-93 7.675670941721776E-4 94-95 7.424009599370242E-4 96-97 2.2649520811638025E-4 98-99 1.0066453694061347E-4 100 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 3973594.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.93054878293343 #Duplication Level Percentage of deduplicated Percentage of total 1 70.58659854441699 38.773605947654346 2 15.857391511759195 17.42110436013526 3 5.830232459356702 9.607736055736057 4 2.818461307660302 6.192785054129782 5 1.509016700748338 4.144555774735892 6 0.8804863280181942 2.9019358316345603 7 0.5501185692641193 2.1152820433752173 8 0.3863228240245176 1.6976739784831496 9 0.2730984354678396 1.3501302238808062 >10 1.233362773107787 11.469434730183686 >50 0.05464188860225084 2.0420201584679294 >100 0.019627569622264057 1.7708499990119193 >500 4.579199866536869E-4 0.15623022482737547 >1k 1.3737597362209158E-4 0.21335446432788033 >5k 4.5791991207363816E-5 0.14330115341616761 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5697 0.1433714667376687 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4776 0.12019345710709246 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 5.033226847030673E-5 0.0 10-11 0.0 0.0 0.0 7.54984027054601E-5 0.0 12-13 0.0 0.0 0.0 7.54984027054601E-5 0.0 14-15 0.0 0.0 0.0 1.635798725284969E-4 0.0 16-17 0.0 0.0 0.0 1.8874600676365024E-4 0.0 18-19 0.0 0.0 0.0 2.1391214099880358E-4 0.0 20-21 0.0 0.0 0.0 2.76827476586687E-4 0.0 22-23 0.0 0.0 0.0 3.1457667793941707E-4 0.0 24-25 0.0 0.0 0.0 3.5232587929214714E-4 0.0 26-27 0.0 0.0 0.0 4.1524121488003055E-4 0.0 28-29 0.0 0.0 0.0 0.001107309906346748 0.0 30-31 0.0 0.0 0.0 0.003523258792921471 0.0 32-33 0.0 0.0 0.0 0.009487632606652818 0.0 34-35 1.2583067117576682E-5 0.0 0.0 0.018522274797072878 0.0 36-37 2.5166134235153364E-5 0.0 0.0 0.035521998472918974 0.0 38-39 2.5166134235153364E-5 0.0 0.0 0.0649663755280484 0.0 40-41 2.5166134235153364E-5 0.0 0.0 0.09775784843645324 0.0 42-43 2.5166134235153364E-5 0.0 0.0 0.13276394115755158 0.0 44-45 2.5166134235153364E-5 1.2583067117576682E-5 0.0 0.17383507222932187 0.0 46-47 2.5166134235153364E-5 2.5166134235153364E-5 0.0 0.22477132792127227 0.0 48-49 2.5166134235153364E-5 2.5166134235153364E-5 0.0 0.2823891922526559 0.0 50-51 2.5166134235153364E-5 2.5166134235153364E-5 0.0 0.34539260931036236 0.0 52-53 2.5166134235153364E-5 2.5166134235153364E-5 0.0 0.40966691614694406 0.0 54-55 2.5166134235153364E-5 2.5166134235153364E-5 0.0 0.48160431085812994 0.0 56-57 2.5166134235153364E-5 5.033226847030673E-5 0.0 0.5678738190162357 0.0 58-59 2.5166134235153364E-5 5.033226847030673E-5 0.0 0.6626746466800584 0.0 60-61 2.5166134235153364E-5 5.033226847030673E-5 0.0 0.7666736964068297 0.0 62-63 2.5166134235153364E-5 5.033226847030673E-5 0.0 0.8743470017319334 0.0 64-65 2.5166134235153364E-5 5.033226847030673E-5 0.0 0.9906648741668123 0.0 66-67 2.5166134235153364E-5 5.033226847030673E-5 0.0 1.123781141203656 0.0 68-69 2.5166134235153364E-5 5.033226847030673E-5 0.0 1.2733183108289372 0.0 70-71 2.5166134235153364E-5 5.033226847030673E-5 0.0 1.437527336713313 0.0 72-73 2.5166134235153364E-5 5.033226847030673E-5 0.0 1.6092232875326467 0.0 74-75 2.5166134235153364E-5 5.033226847030673E-5 0.0 1.7876511792598841 0.0 76-77 2.5166134235153364E-5 5.033226847030673E-5 0.0 1.9760574432113596 0.0 78-79 2.5166134235153364E-5 5.033226847030673E-5 0.0 2.19803533023253 0.0 80-81 2.5166134235153364E-5 5.033226847030673E-5 0.0 2.441064688541406 0.0 82-83 2.5166134235153364E-5 5.033226847030673E-5 0.0 2.6996593008747247 0.0 84-85 2.5166134235153364E-5 5.033226847030673E-5 0.0 2.9570207726305204 0.0 86-87 2.5166134235153364E-5 5.033226847030673E-5 0.0 3.230060746014817 0.0 88 2.5166134235153364E-5 5.033226847030673E-5 0.0 3.4564678726613742 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5240 0.0 51.995808 1 GTATCAA 9250 0.0 43.21414 1 TCAACGC 10730 0.0 36.70539 4 ATCAACG 10750 0.0 36.681286 3 CAACGCA 10925 0.0 36.049335 5 TATCAAC 11380 0.0 34.777237 2 AACGCAG 11495 0.0 34.425304 6 ACGCAGA 13025 0.0 30.164991 7 CGCAGAG 13445 0.0 29.153143 8 GTACATG 10490 0.0 26.871843 1 TACATGG 10510 0.0 25.64197 2 GCAGAGT 15135 0.0 25.49417 9 TATACCG 225 1.1641532E-10 25.065329 5 ACATGGG 10745 0.0 24.101065 3 GAGTACT 9740 0.0 22.533703 12-13 CAGAGTA 15170 0.0 21.051544 10-11 AGAGTAC 13765 0.0 20.929766 10-11 GTACTTT 11155 0.0 19.548811 14-15 CATGGGG 8320 0.0 19.432144 4 AGTACTT 10370 0.0 19.147964 12-13 >>END_MODULE