FastQCFastQC Report
Wed 25 May 2016
SRR1033084_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033084_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2180252
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA17950.043.2116931
TCAACGC28650.033.3083574
GTATCAA30550.033.237191
ATCAACG29250.032.625113
CAACGCA29400.032.45945
AACGCAG31350.030.2876576
TATCAAC34600.027.8522052
GTACATG60050.027.0861
TACATGG61300.025.460062
CGCAGAG38250.024.4710678
ACGCAGA38350.024.268997
ACATGGG63600.023.5784573
CATGGGG43250.020.2166234
CCTAGCG1200.00505990219.5871182
TACGCAG1708.617606E-519.3571265
GCAGAGT46200.019.0392959
CTGTCGC6450.018.2318829
TCTGTCG5700.018.1542848
GAGTACA45950.018.107961
TCGTCGA650.0079904918.09725230-31
Produced by FastQC (version 0.11.5)