##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1033084_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2180252
Sequences flagged as poor quality	0
Sequence length	100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.55313605950138	33.0	31.0	34.0	30.0	34.0
2	31.615455461111836	33.0	31.0	34.0	30.0	34.0
3	31.663042162098694	34.0	31.0	34.0	30.0	34.0
4	35.029143878780985	37.0	35.0	37.0	33.0	37.0
5	35.06589146575717	37.0	35.0	37.0	33.0	37.0
6	35.13125134158803	37.0	35.0	37.0	33.0	37.0
7	35.00656437879658	37.0	35.0	37.0	32.0	37.0
8	35.00587959556969	37.0	35.0	37.0	32.0	37.0
9	36.60313119767807	39.0	37.0	39.0	33.0	39.0
10-11	36.61700711660854	39.0	37.0	39.0	33.0	39.0
12-13	36.586281081269505	39.0	37.0	39.0	33.0	39.0
14-15	37.97522946888708	41.0	38.0	41.0	33.0	41.0
16-17	37.9882942430508	41.0	38.0	41.0	33.0	41.0
18-19	37.996626995411546	41.0	38.5	41.0	33.0	41.0
20-21	38.02207061385565	40.5	39.0	41.0	33.0	41.0
22-23	37.99710996710472	40.0	38.5	41.0	33.0	41.0
24-25	38.01698840317541	40.0	38.0	41.0	33.0	41.0
26-27	37.93697173537738	40.0	38.0	41.0	33.0	41.0
28-29	37.952920121160304	40.0	38.0	41.0	33.0	41.0
30-31	38.10496791196614	40.0	38.0	41.0	33.0	41.0
32-33	38.089209183158644	41.0	38.0	41.0	33.0	41.0
34-35	38.04672372734895	41.0	38.0	41.0	33.0	41.0
36-37	37.97068228810248	40.0	38.0	41.0	33.0	41.0
38-39	37.879582268471715	40.0	38.0	41.0	32.5	41.0
40-41	37.807158759629615	40.0	38.0	41.0	32.0	41.0
42-43	37.68983722982481	40.0	38.0	41.0	32.0	41.0
44-45	37.56499707373276	40.0	38.0	41.0	32.0	41.0
46-47	37.43996083938921	40.0	38.0	41.0	31.0	41.0
48-49	37.32341651332048	40.0	37.5	41.0	31.0	41.0
50-51	37.20608764491444	40.0	37.0	41.0	31.0	41.0
52-53	37.05121632728694	40.0	37.0	41.0	30.5	41.0
54-55	36.89948226168351	40.0	36.5	41.0	30.0	41.0
56-57	36.73464053696546	40.0	36.0	41.0	30.0	41.0
58-59	36.54037767193884	40.0	36.0	41.0	30.0	41.0
60-61	36.32261270715495	39.0	35.0	41.0	30.0	41.0
62-63	36.0743322331547	39.0	35.0	41.0	30.0	41.0
64-65	35.8065157146972	39.0	35.0	41.0	29.5	41.0
66-67	35.517722263298	38.0	35.0	40.0	29.0	41.0
68-69	35.209540915453815	37.5	35.0	40.0	29.0	41.0
70-71	34.8763418173679	37.0	34.0	40.0	29.0	41.0
72-73	34.52963533573183	36.5	34.0	39.0	28.0	41.0
74-75	34.183129748304324	36.0	34.0	39.0	28.0	41.0
76-77	33.81865949440707	35.5	34.0	38.5	28.0	40.0
78-79	33.43789800445086	35.0	34.0	37.0	27.0	39.5
80-81	33.08119497195737	35.0	33.5	37.0	27.0	39.0
82-83	32.85039825671528	35.0	33.0	36.5	27.0	39.0
84-85	32.62017853899458	35.0	33.5	36.0	27.0	37.5
86-87	32.43224636418175	35.0	33.5	36.0	27.0	37.0
88-89	32.20499556931951	35.0	33.0	35.5	26.5	37.0
90-91	31.987389989781	35.0	33.0	35.0	26.0	36.0
92-93	31.76067009685119	35.0	33.0	35.0	26.0	36.0
94-95	31.556178827034675	35.0	33.0	35.0	25.0	36.0
96-97	31.380906656661708	35.0	33.0	35.0	25.0	36.0
98-99	31.203939269405556	35.0	33.0	35.0	24.5	35.5
100	31.062071265156504	35.0	33.0	35.0	24.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.097670551153751
1101	2	0.19260586928064427
1101	3	0.13064951607291775
1101	4	-0.010771745573812552
1101	5	0.08746476365922007
1101	6	0.1765281922609887
1101	7	0.1543118477303338
1101	8	0.22630389562843334
1101	9	0.17504904018304757
1101	10-11	0.21130977608117263
1101	12-13	0.23348240340109783
1101	14-15	0.29305391863207575
1101	16-17	0.25684510808588357
1101	18-19	0.2425987177539639
1101	20-21	0.1588443451636934
1101	22-23	0.18516476954032157
1101	24-25	0.11683995011747328
1101	26-27	0.15918072559930607
1101	28-29	0.12494627315226126
1101	30-31	0.2245998392346138
1101	32-33	0.12362288483224404
1101	34-35	0.19679330133335782
1101	36-37	0.16009618916583435
1101	38-39	0.20886557698359098
1101	40-41	0.23433308044717194
1101	42-43	0.24306625190972397
1101	44-45	0.2573904872289887
1101	46-47	0.18847751343385966
1101	48-49	0.13554486632165919
1101	50-51	0.14175531655721585
1101	52-53	0.09408460228895166
1101	54-55	0.13342797593724498
1101	56-57	0.20796069279131757
1101	58-59	0.15331336464934253
1101	60-61	0.11538696803105353
1101	62-63	0.11307678975860824
1101	64-65	0.1325535367595876
1101	66-67	0.09008381874302529
1101	68-69	0.14323121049508813
1101	70-71	0.1779142032277221
1101	72-73	0.011547066014152563
1101	74-75	0.0705197499864596
1101	76-77	0.08274857328746066
1101	78-79	0.10554764865601385
1101	80-81	0.18157691407744636
1101	82-83	0.06875459945112539
1101	84-85	0.09054836941911049
1101	86-87	0.08334442383841179
1101	88-89	0.20064308960367327
1101	90-91	0.18061835496455103
1101	92-93	0.13736257485710013
1101	94-95	0.19980396752065133
1101	96-97	0.21873152044077315
1101	98-99	0.14587537903843284
1101	100	0.09700723781322296
1102	1	0.12859052168562002
1102	2	0.1133806718723882
1102	3	0.12820653371639779
1102	4	0.014547811798529153
1102	5	0.05606092293671594
1102	6	0.23403590057238688
1102	7	0.16034909770492334
1102	8	0.1435069148499437
1102	9	0.10604591601975244
1102	10-11	0.1198273368523104
1102	12-13	0.1516472551530441
1102	14-15	0.2766739146537489
1102	16-17	0.2641925817167987
1102	18-19	0.17765428711361864
1102	20-21	0.250640129917862
1102	22-23	0.2203274946953968
1102	24-25	0.24311532892542687
1102	26-27	0.1725617783071982
1102	28-29	0.18720091092987445
1102	30-31	0.23459815979896348
1102	32-33	0.26220385806520596
1102	34-35	0.28858254730116073
1102	36-37	0.33266353694460804
1102	38-39	0.3770487832235645
1102	40-41	0.29411152571650234
1102	42-43	0.2933530751998177
1102	44-45	0.22973474767734103
1102	46-47	0.23739767301695736
1102	48-49	0.15752545553284136
1102	50-51	0.21121231279477826
1102	52-53	0.21895746878047362
1102	54-55	0.24293085151195015
1102	56-57	0.26076724314100375
1102	58-59	0.18743911858862106
1102	60-61	0.21502224141082849
1102	62-63	0.22398699570284464
1102	64-65	0.2499380015668109
1102	66-67	0.20773723415763357
1102	68-69	0.2825898404426894
1102	70-71	0.2224229189710769
1102	72-73	0.21614764138146114
1102	74-75	0.18740170332613815
1102	76-77	0.11614777628970074
1102	78-79	0.03084878271100422
1102	80-81	0.22977780716094998
1102	82-83	0.11066569627632816
1102	84-85	0.12326713713549964
1102	86-87	0.16827895682108363
1102	88-89	0.10758262326660173
1102	90-91	0.10717499335842717
1102	92-93	0.1554674418478612
1102	94-95	0.25181226971444914
1102	96-97	0.16450203840672017
1102	98-99	0.1535629269178358
1102	100	0.16344692829231633
1103	1	-0.02396518660850333
1103	2	0.062391624364146026
1103	3	-0.0015930771809031796
1103	4	-0.015575815967906692
1103	5	0.013577828536448067
1103	6	0.040546403178630896
1103	7	-0.030546786264665116
1103	8	-0.04890550532227422
1103	9	0.1111828307176026
1103	10-11	0.09124159485235595
1103	12-13	0.1628484389891156
1103	14-15	0.12452114489814647
1103	16-17	0.1383895319398718
1103	18-19	0.09257092396261868
1103	20-21	0.14598556089604386
1103	22-23	0.1892191389836455
1103	24-25	0.07565144637428745
1103	26-27	0.057107860651200326
1103	28-29	0.06761792142382461
1103	30-31	0.0887086459434272
1103	32-33	0.06944394202029969
1103	34-35	-0.011845481112402467
1103	36-37	0.08374654460055098
1103	38-39	0.09276599010039632
1103	40-41	0.12233823094257446
1103	42-43	0.07597137467782034
1103	44-45	0.07733825085941959
1103	46-47	0.09062990320121855
1103	48-49	0.10807672113167399
1103	50-51	0.06803976539901413
1103	52-53	0.10207980553271767
1103	54-55	0.14197223954473515
1103	56-57	0.16447105595244693
1103	58-59	0.1317015308774998
1103	60-61	0.08988483462483998
1103	62-63	-0.009466568223430727
1103	64-65	0.040345140507895394
1103	66-67	0.08526113049826733
1103	68-69	0.07067478677142702
1103	70-71	0.04856768313673854
1103	72-73	0.04410270512838821
1103	74-75	0.08559754932991837
1103	76-77	-0.008190318272205843
1103	78-79	-0.03493733305526803
1103	80-81	-0.001695046841021508
1103	82-83	-0.040408175752290276
1103	84-85	-0.03209821789561573
1103	86-87	-0.0079413914206512
1103	88-89	-0.007418705702370687
1103	90-91	0.06802607193067445
1103	92-93	0.09039904656162534
1103	94-95	0.10709521653240728
1103	96-97	0.045638038217827415
1103	98-99	-0.004066805886029812
1103	100	0.017462803320462683
1104	1	-0.10975522226865664
1104	2	0.006604380321569181
1104	3	-0.044585880485421114
1104	4	-0.11027947077887035
1104	5	-0.0975695578313136
1104	6	-0.03825480999212516
1104	7	-0.049914303507243574
1104	8	0.07150626615622713
1104	9	0.039296312337874895
1104	10-11	0.022637671762204548
1104	12-13	0.12067719138840971
1104	14-15	0.13768464694296512
1104	16-17	0.09553820213628228
1104	18-19	0.05845104843405835
1104	20-21	0.07444381517297671
1104	22-23	0.05955828108959338
1104	24-25	0.09247890705900375
1104	26-27	0.06507769225514437
1104	28-29	0.09378373719914634
1104	30-31	0.11832013794473539
1104	32-33	0.044252511182236276
1104	34-35	0.15249988456584163
1104	36-37	0.1398350309918115
1104	38-39	0.04156725058670929
1104	40-41	0.1468841199308244
1104	42-43	0.10981950121853856
1104	44-45	0.09394443247859385
1104	46-47	0.10785087156131112
1104	48-49	0.1312329523533151
1104	50-51	0.06598910936889268
1104	52-53	0.10810197129170973
1104	54-55	0.0954624712490002
1104	56-57	0.11560292281910023
1104	58-59	0.14982070613587695
1104	60-61	0.2012287382525244
1104	62-63	0.1330977138971292
1104	64-65	0.10881169077830322
1104	66-67	0.21257103632454744
1104	68-69	0.2380060792272758
1104	70-71	0.2135764128560993
1104	72-73	0.21873604902886967
1104	74-75	0.21110536349335263
1104	76-77	0.13419637280929209
1104	78-79	0.159160998125202
1104	80-81	0.07215301661228324
1104	82-83	0.02218888027405086
1104	84-85	0.15227408610365245
1104	86-87	0.10743915967936601
1104	88-89	0.12411190509409664
1104	90-91	0.10111367982984376
1104	92-93	0.048027654175328394
1104	94-95	0.14968426045859218
1104	96-97	0.1987899637242272
1104	98-99	0.20573776511174557
1104	100	0.26112496396937956
1105	1	-0.17631746947262883
1105	2	-0.17343207542688788
1105	3	-0.19686497185396945
1105	4	-0.18904634977523216
1105	5	-0.15258404451403607
1105	6	-0.17876849911049675
1105	7	-0.08286592906633672
1105	8	-0.18985200643017208
1105	9	-0.15658579687456609
1105	10-11	-0.07647096849300539
1105	12-13	-0.11953720240956045
1105	14-15	-0.07553928879050176
1105	16-17	-0.12468517659220169
1105	18-19	-0.14172485586428962
1105	20-21	-0.14741430488209062
1105	22-23	-0.13441711052134053
1105	24-25	-0.1813843550475056
1105	26-27	-0.2244582026940094
1105	28-29	-0.12858524900625667
1105	30-31	-0.02484668580644467
1105	32-33	-0.11249977033749303
1105	34-35	-0.15049064027854797
1105	36-37	-0.05641345697491573
1105	38-39	-0.07672558226171589
1105	40-41	-0.12373242539374729
1105	42-43	-0.12763399948948972
1105	44-45	-0.16799436982410043
1105	46-47	-0.26999474851271543
1105	48-49	-0.18668105639788735
1105	50-51	-0.17983267028479588
1105	52-53	-0.14687775949427362
1105	54-55	-0.18066842340420664
1105	56-57	-0.14021504734546397
1105	58-59	-0.07162413747017382
1105	60-61	-0.17852967032201406
1105	62-63	-0.18137348803463738
1105	64-65	-0.11019690106149937
1105	66-67	-0.12382081592190275
1105	68-69	-0.08612817263307448
1105	70-71	-0.07878108417294527
1105	72-73	-0.011921232027958695
1105	74-75	-0.09688818921191
1105	76-77	-0.05620371461165519
1105	78-79	-0.002757163120904238
1105	80-81	0.03862920882301779
1105	82-83	-0.012457133194239134
1105	84-85	-0.02725013754219674
1105	86-87	-0.07162200614572356
1105	88-89	-0.05911966479485642
1105	90-91	0.05313627571590018
1105	92-93	0.07878540861049999
1105	94-95	0.0836348524471262
1105	96-97	0.10760718565049032
1105	98-99	0.07671340411180694
1105	100	0.05778505888788743
1106	1	0.004078597547632512
1106	2	0.029089118820149196
1106	3	0.12641514733761383
1106	4	0.010680987707004874
1106	5	-0.063319761426051
1106	6	-0.010365048874952265
1106	7	0.1445582926161535
1106	8	-0.032669907000787646
1106	9	0.13304963181728624
1106	10-11	0.13547967476538503
1106	12-13	0.08456733949766004
1106	14-15	0.03817467067614899
1106	16-17	0.0013659737052265086
1106	18-19	0.08728101079709205
1106	20-21	0.08584043268266583
1106	22-23	0.04985639963983601
1106	24-25	-0.022459585636767088
1106	26-27	-0.04555631148211603
1106	28-29	-0.07931208473263496
1106	30-31	-0.0354219906812574
1106	32-33	-0.10864022967420794
1106	34-35	-0.0918161942069915
1106	36-37	-0.015121684243361244
1106	38-39	0.02264722778609496
1106	40-41	0.013242738907699447
1106	42-43	0.08586194866416008
1106	44-45	0.05319930316788657
1106	46-47	-0.03279616323655432
1106	48-49	-0.02990336810006511
1106	50-51	0.01371091791852308
1106	52-53	-0.022380038314274486
1106	54-55	0.01946984404654728
1106	56-57	0.08560909175211151
1106	58-59	0.01676963764376893
1106	60-61	0.05340728921654403
1106	62-63	0.020189583123723764
1106	64-65	-0.012754639713932647
1106	66-67	0.056889857953621004
1106	68-69	0.057148323252711464
1106	70-71	0.07915690845531742
1106	72-73	0.062096367080499704
1106	74-75	0.08894882021033368
1106	76-77	0.11601422820491081
1106	78-79	0.08522446582358612
1106	80-81	0.017767763005302584
1106	82-83	0.004212749107672664
1106	84-85	-0.042760396586182026
1106	86-87	-0.04715744346773931
1106	88-89	-0.038072286179733794
1106	90-91	-0.036161088037246714
1106	92-93	-0.02357171526696078
1106	94-95	-0.0649935112849036
1106	96-97	-0.12375616488381169
1106	98-99	-0.139339630810575
1106	100	-0.06971745694534803
1107	1	-0.16081108548429413
1107	2	-0.10633698097610278
1107	3	-0.12682145646436993
1107	4	-0.03916417025388341
1107	5	-0.08913836240824935
1107	6	-0.06424750470168306
1107	7	-0.029852247814218913
1107	8	-0.04543074862454688
1107	9	-0.10157272920278615
1107	10-11	-0.11014580100005134
1107	12-13	-0.05158757956847637
1107	14-15	0.003065687226801117
1107	16-17	-0.11066605797395823
1107	18-19	-0.07717206142022093
1107	20-21	-0.0761891790796696
1107	22-23	-0.1353031409545764
1107	24-25	-0.12477362295421557
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2216	26-27	-0.4105558590179186
2216	28-29	-0.4106223094758832
2216	30-31	-0.5419628498802069
2216	32-33	-0.551977457358781
2216	34-35	-0.6646057613607752
2216	36-37	-0.7283212879091749
2216	38-39	-0.631831464665737
2216	40-41	-0.5018149181776295
2216	42-43	-0.5292802932409302
2216	44-45	-0.4585933890461007
2216	46-47	-0.5200566208918644
2216	48-49	-0.4874833969416059
2216	50-51	-0.41028514656007076
2216	52-53	-0.45780456896672916
2216	54-55	-0.4735367943370292
2216	56-57	-0.5850557050349039
2216	58-59	-0.5931217266183495
2216	60-61	-0.5081745027067939
2216	62-63	-0.5928950166762732
2216	64-65	-0.6173185437095867
2216	66-67	-0.6138910356448406
2216	68-69	-0.5733460214033315
2216	70-71	-0.5576365862711867
2216	72-73	-0.5244676014218825
2216	74-75	-0.5878409432150917
2216	76-77	-0.44995065782149624
2216	78-79	-0.46575697641829095
2216	80-81	-0.4323203910844313
2216	82-83	-0.6005821256346451
2216	84-85	-0.4256030839545133
2216	86-87	-0.38227864731600647
2216	88-89	-0.46026904115538514
2216	90-91	-0.3751036272235524
2216	92-93	-0.6315840919972118
2216	94-95	-0.571150960572993
2216	96-97	-0.5193338470258801
2216	98-99	-0.5005540325015687
2216	100	-0.4480584694318317
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	27359.0
3	5425.0
4	2873.0
5	2529.0
6	2106.0
7	2197.0
8	2550.0
9	2904.0
10	3287.0
11	3655.0
12	3880.0
13	4429.0
14	4884.0
15	4979.0
16	5000.0
17	5007.0
18	4880.0
19	4946.0
20	4765.0
21	5052.0
22	5872.0
23	8493.0
24	13849.0
25	18437.0
26	18758.0
27	15664.0
28	16227.0
29	20304.0
30	24690.0
31	29737.0
32	38819.0
33	51725.0
34	74720.0
35	113806.0
36	205942.0
37	426136.0
38	687910.0
39	305704.0
40	752.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.7657539489856	20.220348301952257	13.523525939986616	23.49037180907553
2	15.314452162469475	20.379853476828032	40.70423078087875	23.601463579823744
3	17.666981798823304	25.93507053759098	30.294908626972255	26.103039036613463
4	11.99191031577034	17.18911013932489	39.83549812561135	30.98348141929342
5	12.432705890022131	38.33024760927159	35.925678197465835	13.311368303240446
6	30.09667020759902	38.931724887000804	18.110308152927878	12.8612967524723
7	26.625453797576444	33.10876818633383	22.992135093495612	17.273642922594114
8	24.558746961664866	36.99261984148775	20.769081020127622	17.679552176719763
9	24.449455906429314	17.656160042752507	21.665584692840334	36.228799357977856
10-11	23.224462519562014	27.599328653066024	29.248311359523456	19.92789746784851
12-13	23.974954021381464	24.978368067249658	29.176902950046934	21.869774961321944
14-15	21.37700365646284	27.120499356153072	26.186559726932686	25.315937260451406
16-17	21.042481454340486	30.29615908679366	26.673206005940948	21.988153452924905
18-19	20.922722273579456	29.19730214221258	28.705519975336287	21.174455608871686
20-21	22.267768625394098	28.531442206274093	28.13550373753152	21.06528543080029
22-23	21.924545057153967	28.346372676237646	28.143388026292087	21.585694240316293
24-25	21.68414586507868	28.3255684113293	28.2010375810603	21.78924814253172
26-27	21.501625013957245	28.225433294934632	28.317493678459066	21.955448012649057
28-29	21.50837245251082	28.31616495594847	28.30866086656398	21.86680172497673
30-31	21.834633002940237	28.76439622109309	27.895568026039143	21.505402749927534
32-33	21.354041879751083	28.431313150785282	28.51422903602768	21.700415933435963
34-35	21.48828083155965	28.0942121086796	28.579352031979322	21.83815502778143
36-37	21.934609875083204	28.611363318779098	27.864285581530456	21.589741224607238
38-39	21.659270044379735	28.752316010694102	28.051402183390177	21.537011761535986
40-41	21.398738789398486	28.33069194443329	28.445405876851222	21.825163389317005
42-43	21.380373511968628	28.249169245358758	28.41597426594201	21.954482976730606
44-45	21.642946091326245	28.46551661618385	28.36235540791938	21.529181884570527
46-47	21.576404449768198	28.679058552106255	28.102399109844384	21.642137888281155
48-49	21.409361562273936	28.481392364158474	28.23249033309954	21.876755740468052
50-51	21.54982623624939	28.31652611115051	28.261561548949558	21.872086103650542
52-53	21.576919378340893	28.336866582713267	28.266890113993483	21.81932392495236
54-55	21.704019717637554	28.78114156676275	27.98979694872313	21.52504176687657
56-57	21.451065079794922	28.504206617677436	28.138279073396642	21.906449229131
58-59	21.34141527818958	28.118164135111346	28.48798356500882	22.05243702169026
60-61	21.78361848428021	28.555069977410056	27.94444857507139	21.716862963238338
62-63	21.499658742861026	28.517966373664404	28.08286925881336	21.89950562466121
64-65	21.46918547052934	28.202730374330105	28.33977920267966	21.9883049524609
66-67	21.36482595097932	28.353568140322405	28.086672474267406	22.194933434430872
68-69	21.872958989076817	28.470722580402345	28.002956726322896	21.653361704197938
70-71	21.775046473402483	28.173793200300423	28.13258039651949	21.918579929777604
72-73	21.437010904970514	28.29977872476348	28.38254754962286	21.880662820643145
74-75	21.52458732375136	28.441554120337276	28.1665551816441	21.86730337426727
76-77	21.55257235375968	28.420374150534002	28.056356630699362	21.970696865006957
78-79	21.65699712150815	28.491011739978912	28.111748353959275	21.74024278455366
80-81	21.56190574959068	28.28599826726676	28.24469901102048	21.907396972122083
82-83	21.386107989921534	28.311554096952346	28.430089190430092	21.87224872269603
84-85	21.476578280568734	28.24461685438732	28.250673839605522	22.02813102543842
86-87	21.619760786088857	28.392708548181762	28.147703910323575	21.839826755405806
88-89	21.33517796445873	28.319814068524373	28.200119533932376	22.144888433084514
90-91	21.33550794993824	28.15689542201756	28.318041578645015	22.18955504939919
92-93	21.550885542516962	28.395657955474636	28.104102063833945	21.94935443817446
94-95	21.238479405402874	28.166024122700357	28.321712173952147	22.273784297944623
96-97	21.24181102105675	28.149232253828178	28.408237320131548	22.200719404983523
98-99	21.27240101840905	28.203745133430445	28.322545756479833	22.201308091680673
100	21.50595810898932	28.109094863961825	28.17402900514012	22.210918021908732
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	617.0
1	524.0
2	416.0
3	376.5
4	329.0
5	326.0
6	336.5
7	311.5
8	281.5
9	262.0
10	251.5
11	243.0
12	255.5
13	254.5
14	279.5
15	355.0
16	465.0
17	633.5
18	835.0
19	1168.5
20	1713.0
21	2514.5
22	3785.0
23	5851.0
24	8375.0
25	11538.0
26	15753.0
27	20911.5
28	27341.0
29	34116.0
30	40429.5
31	47231.5
32	53485.5
33	59298.0
34	65378.0
35	69767.5
36	72724.0
37	75609.0
38	77848.5
39	78140.0
40	78395.0
41	78368.0
42	76464.5
43	75123.0
44	74185.5
45	72408.0
46	71160.5
47	69540.5
48	67620.5
49	66105.5
50	64307.5
51	61811.5
52	57733.0
53	54905.0
54	53194.5
55	50257.0
56	46418.0
57	42074.5
58	38216.5
59	34962.0
60	31371.5
61	27013.5
62	22953.0
63	19215.5
64	15573.0
65	12475.0
66	9775.0
67	7398.5
68	5578.5
69	4277.0
70	3048.0
71	2191.5
72	1754.5
73	1298.0
74	920.0
75	653.0
76	438.5
77	320.5
78	212.5
79	137.0
80	87.5
81	61.0
82	52.5
83	39.5
84	29.5
85	22.0
86	15.0
87	11.5
88	10.5
89	9.0
90	5.0
91	5.0
92	4.0
93	1.5
94	1.5
95	2.5
96	3.0
97	2.0
98	2.5
99	1.5
100	4.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.06136905275170026
2	0.043022549686916926
3	0.058341879746011016
4	0.07682598158378022
5	0.08902640612186115
6	0.08797148219563611
7	0.09026479507873401
8	0.10319907973940627
9	0.0978785938506191
10-11	0.10285508280694158
12-13	0.10721237728482762
14-15	0.10675371470820803
16-17	0.07533532820976657
18-19	0.09877298587502728
20-21	0.0921682447717053
22-23	0.05822721410185612
24-25	0.0215800742299514
26-27	0.1817679791143409
28-29	0.06604741103322001
30-31	0.0773075772892308
32-33	0.07054230428409193
34-35	0.021488341714627483
36-37	0.05327365827436461
38-39	0.07648198465131553
40-41	0.08207766808607446
42-43	0.07616092084768183
44-45	0.0744409361853584
46-47	0.08056408158322982
48-49	0.07723877790273784
50-51	0.07226228894641537
52-53	0.07625265336300575
54-55	0.06843245643164185
56-57	0.07072576931473977
58-59	0.0681572588856701
60-61	0.06448795827271343
62-63	0.07077163557240172
64-65	0.06726286686126191
66-67	0.0687076539776136
68-69	0.0683865901739799
70-71	0.060428794469630114
72-73	0.0592362717704192
74-75	0.0612085208498834
76-77	0.05669069447018051
78-79	0.0531819257590407
80-81	0.04751744293778884
82-83	0.05276912944008307
84-85	0.04357294477886042
86-87	0.04153189631290328
88-89	0.04377934293833924
90-91	0.039628446619932006
92-93	0.041738294472382094
94-95	0.04100443434979076
96-97	0.04361881103652238
98-99	0.044513203060930574
100	0.051416074839055304
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	2180252.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	73.32854729946676
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.56205991108797	60.541559153316236
2	11.59711793713484	17.00799622381373
3	2.9433070352045387	6.47485287443548
4	1.128170534260596	3.309084255335712
5	0.5467109031583516	2.004475816069069
6	0.31470435756385456	1.3846088021381646
7	0.20310798858179996	1.042552962333405
8	0.13150240842972571	0.7714304461226351
9	0.09984075221373492	0.6589059589007282
>10	0.4547704047824206	5.63975974792784
>50	0.015320330192604993	0.7513209276893849
>100	0.003262265212618112	0.3486512529945283
>500	1.2517217695590872E-4	0.06480157892316578
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	4.586625766195834E-5	0.0
8	0.0	0.0	0.0	4.586625766195834E-5	0.0
9	0.0	0.0	0.0	2.7519754597175004E-4	0.0
10-11	0.0	0.0	0.0	2.7519754597175004E-4	0.0
12-13	0.0	0.0	0.0	2.7519754597175004E-4	0.0
14-15	0.0	0.0	0.0	2.7519754597175004E-4	0.0
16-17	0.0	0.0	0.0	2.7519754597175004E-4	0.0
18-19	0.0	0.0	0.0	2.7519754597175004E-4	0.0
20-21	0.0	0.0	0.0	2.7519754597175004E-4	0.0
22-23	0.0	0.0	0.0	3.210638036337084E-4	0.0
24-25	0.0	0.0	0.0	4.3572944778860425E-4	0.0
26-27	0.0	0.0	0.0	5.962613496054585E-4	0.0
28-29	0.0	0.0	0.0	0.001192522699210917	0.0
30-31	0.0	0.0	0.0	0.003210638036337084	0.0
32-33	0.0	0.0	0.0	0.007957795704349773	0.0
34-35	0.0	0.0	0.0	0.015525728218572898	0.0
36-37	0.0	0.0	0.0	0.02710695827821738	0.0
38-39	0.0	0.0	0.0	0.048251303060380175	0.0
40-41	0.0	0.0	0.0	0.07600038894586497	0.0
42-43	0.0	0.0	0.0	0.10652438341989826	0.0
44-45	0.0	0.0	0.0	0.13886009507157887	0.0
46-47	0.0	0.0	0.0	0.17997919506552454	0.0
48-49	0.0	0.0	0.0	0.22765716990513024	0.0
50-51	0.0	0.0	0.0	0.2827196122283112	0.0
52-53	0.0	0.0	0.0	0.3385159146740836	0.0
54-55	0.0	0.0	0.0	0.40025189748707946	2.293312883097917E-5
56-57	0.0	0.0	0.0	0.4695099465566366	4.586625766195834E-5
58-59	0.0	0.0	0.0	0.5524820066671192	4.586625766195834E-5
60-61	0.0	0.0	0.0	0.6425404035863744	4.586625766195834E-5
62-63	0.0	0.0	0.0	0.7359929035726145	4.586625766195834E-5
64-65	0.0	0.0	0.0	0.8377701293245001	4.586625766195834E-5
66-67	0.0	0.0	0.0	0.9500736612098051	4.586625766195834E-5
68-69	0.0	0.0	0.0	1.0781551857308238	4.586625766195834E-5
70-71	0.0	0.0	0.0	1.222060569145218	4.586625766195834E-5
72-73	0.0	0.0	0.0	1.3808036869132558	4.586625766195834E-5
74-75	0.0	0.0	0.0	1.5384918807550685	4.586625766195834E-5
76-77	0.0	0.0	0.0	1.7005373690747674	4.586625766195834E-5
78-79	0.0	0.0	0.0	1.8938177788622599	4.586625766195834E-5
80-81	0.0	0.0	0.0	2.105215360426226	4.586625766195834E-5
82-83	0.0	0.0	0.0	2.336564764073144	4.586625766195834E-5
84-85	0.0	0.0	0.0	2.5612864934879087	4.586625766195834E-5
86-87	0.0	2.293312883097917E-5	0.0	2.7973601216740085	4.586625766195834E-5
88	0.0	4.586625766195834E-5	0.0	2.996167415509767	4.586625766195834E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1795	0.0	43.211693	1
TCAACGC	2865	0.0	33.308357	4
GTATCAA	3055	0.0	33.23719	1
ATCAACG	2925	0.0	32.62511	3
CAACGCA	2940	0.0	32.4594	5
AACGCAG	3135	0.0	30.287657	6
TATCAAC	3460	0.0	27.852205	2
GTACATG	6005	0.0	27.086	1
TACATGG	6130	0.0	25.46006	2
CGCAGAG	3825	0.0	24.471067	8
ACGCAGA	3835	0.0	24.26899	7
ACATGGG	6360	0.0	23.578457	3
CATGGGG	4325	0.0	20.216623	4
CCTAGCG	120	0.005059902	19.587118	2
TACGCAG	170	8.617606E-5	19.357126	5
GCAGAGT	4620	0.0	19.039295	9
CTGTCGC	645	0.0	18.231882	9
TCTGTCG	570	0.0	18.154284	8
GAGTACA	4595	0.0	18.10796	1
TCGTCGA	65	0.00799049	18.097252	30-31
>>END_MODULE