FastQCFastQC Report
Wed 25 May 2016
SRR1033084_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033084_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2180252
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48270.22139642573427293No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39840.18273117052524204No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA32500.057.13481
GTATCAA60000.042.3375171
ATCAACG73500.034.2103423
TCAACGC73600.034.163084
CAACGCA75550.033.1561285
TATCAAC76300.033.032192
AACGCAG78100.032.1939166
ACGCAGA91700.027.2654917
GTACATG57400.026.8487281
CGCAGAG92950.026.79778
TACATGG57650.025.0051942
GCAGAGT102150.024.0621479
ATAACGC2151.880835E-924.0461313
ACATGGG60550.023.286173
TAACGCA2052.9906005E-822.9259414
TATAACG1852.7546594E-722.9181142
TGGTATC14750.022.357032
GTGGTAT15200.022.0141851
CGTACAC1503.293522E-521.9329853
GAGTACT76100.020.56532712-13
Produced by FastQC (version 0.11.5)