##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1033084_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2180252
Sequences flagged as poor quality	0
Sequence length	100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.674766036219665	34.0	31.0	34.0	31.0	34.0
2	32.67008171532465	34.0	31.0	34.0	31.0	34.0
3	32.767557603433	34.0	31.0	34.0	31.0	34.0
4	36.279355780891386	37.0	37.0	37.0	35.0	37.0
5	36.26353283932316	37.0	37.0	37.0	35.0	37.0
6	36.332566143730176	37.0	37.0	37.0	35.0	37.0
7	36.28315258970064	37.0	37.0	37.0	35.0	37.0
8	36.28482624944272	37.0	37.0	37.0	35.0	37.0
9	38.07152063156002	39.0	39.0	39.0	35.0	39.0
10-11	38.03615017897014	39.0	38.5	39.0	35.0	39.0
12-13	38.01122484923761	39.0	38.0	39.0	35.0	39.0
14-15	39.49125376332644	41.0	39.0	41.0	36.0	41.0
16-17	39.49664465392074	41.0	39.0	41.0	36.0	41.0
18-19	39.52130487668398	41.0	39.5	41.0	36.5	41.0
20-21	39.52065426381905	41.0	39.5	41.0	37.0	41.0
22-23	39.44443463416155	41.0	39.0	41.0	36.0	41.0
24-25	39.38927701935373	41.0	39.0	41.0	36.0	41.0
26-27	39.27669760192859	41.0	39.0	41.0	36.0	41.0
28-29	39.21968102769772	41.0	39.0	41.0	36.0	41.0
30-31	39.19960720136938	41.0	39.0	41.0	36.0	41.0
32-33	39.139174966930426	41.0	39.0	41.0	35.0	41.0
34-35	39.04950941450805	41.0	39.0	41.0	35.0	41.0
36-37	38.9490889126578	41.0	39.0	41.0	35.0	41.0
38-39	38.84302548512741	41.0	39.0	41.0	35.0	41.0
40-41	38.732632512205015	41.0	39.0	41.0	35.0	41.0
42-43	38.60031638544535	41.0	38.0	41.0	35.0	41.0
44-45	38.415827849257795	41.0	38.0	41.0	35.0	41.0
46-47	38.18933017834635	40.0	38.0	41.0	34.0	41.0
48-49	37.95797550007981	40.0	38.0	41.0	33.5	41.0
50-51	37.8093328202428	40.0	38.0	41.0	33.0	41.0
52-53	37.67225508794397	40.0	38.0	41.0	33.0	41.0
54-55	37.52725510629047	40.0	37.0	41.0	33.0	41.0
56-57	37.38168431906037	40.0	37.0	41.0	33.0	41.0
58-59	37.207807858908055	40.0	36.5	41.0	33.0	41.0
60-61	37.01776767089309	40.0	36.0	41.0	32.0	41.0
62-63	36.791545197527626	39.0	35.5	41.0	32.0	41.0
64-65	36.531052832424876	39.0	35.0	41.0	31.5	41.0
66-67	36.25714642160631	39.0	35.0	41.0	31.0	41.0
68-69	35.95879879940484	38.0	35.0	40.5	31.0	41.0
70-71	35.639897360488604	37.0	35.0	40.0	31.0	41.0
72-73	35.30105854736058	37.0	35.0	39.5	31.0	41.0
74-75	34.96518246514623	36.0	35.0	39.0	30.0	41.0
76-77	34.61225995894053	36.0	35.0	39.0	30.0	40.5
78-79	34.25173672584637	35.0	34.0	37.5	30.0	39.5
80-81	33.9225662905022	35.0	34.0	37.0	29.5	39.0
82-83	33.61823128702554	35.0	34.0	37.0	29.0	39.0
84-85	33.3897267380101	35.0	34.0	36.0	29.0	38.0
86-87	33.16940771066831	35.0	34.0	36.0	29.0	37.0
88-89	32.95134209256545	35.0	34.0	36.0	29.0	37.0
90-91	32.75859877665518	35.0	34.0	35.0	29.0	36.5
92-93	32.5725686755476	35.0	34.0	35.0	29.0	36.0
94-95	32.408166808240516	35.0	34.0	35.0	29.0	36.0
96-97	32.25895424015205	35.0	34.0	35.0	29.0	36.0
98-99	32.127053661686816	35.0	34.0	35.0	29.0	36.0
100	32.01531749540879	35.0	34.0	35.0	29.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.17702430662188817
1101	2	0.15660790362542798
1101	3	0.1806225075704404
1101	4	0.17276528669810176
1101	5	0.14752260342363144
1101	6	0.1720024470854895
1101	7	0.1919493007540396
1101	8	0.139335696750841
1101	9	0.2006159171092392
1101	10-11	0.21771253266184942
1101	12-13	0.27167376764958107
1101	14-15	0.35728526796329874
1101	16-17	0.32518909838218946
1101	18-19	0.3354074996664025
1101	20-21	0.30673118783714415
1101	22-23	0.30896097117623356
1101	24-25	0.3793971557427511
1101	26-27	0.3638159770973388
1101	28-29	0.36910329567555067
1101	30-31	0.37108673215524135
1101	32-33	0.41376635336789036
1101	34-35	0.4253599741675629
1101	36-37	0.41773827517315
1101	38-39	0.3715805696026706
1101	40-41	0.4596752357750731
1101	42-43	0.5004618448527793
1101	44-45	0.47218753590977514
1101	46-47	0.4077347353806786
1101	48-49	0.31864421169100865
1101	50-51	0.35757807588014856
1101	52-53	0.42200780693506346
1101	54-55	0.5178655649146506
1101	56-57	0.4428961953082151
1101	58-59	0.4212947416095645
1101	60-61	0.3804845072278269
1101	62-63	0.3809104077590817
1101	64-65	0.3171645128734397
1101	66-67	0.40398942525403925
1101	68-69	0.37410103615859214
1101	70-71	0.3730302339220799
1101	72-73	0.3946722845944848
1101	74-75	0.3890899258688094
1101	76-77	0.31388263870245225
1101	78-79	0.2769789433668777
1101	80-81	0.35810105165395356
1101	82-83	0.33474099421508186
1101	84-85	0.3311076840045857
1101	86-87	0.3304300154501334
1101	88-89	0.3875401109328891
1101	90-91	0.3803081612135273
1101	92-93	0.26121184784258134
1101	94-95	0.28461301203081746
1101	96-97	0.3204713140923019
1101	98-99	0.3136015762535038
1101	100	0.3766720646074262
1102	1	0.14688807272881377
1102	2	0.06675855496884964
1102	3	0.13114637907342086
1102	4	0.06596304460561697
1102	5	0.08047698540851655
1102	6	0.07581930875711862
1102	7	0.09620403168068492
1102	8	0.09553867628076773
1102	9	0.17505006545067658
1102	10-11	0.18851421310699834
1102	12-13	0.19400141728627318
1102	14-15	0.2624163109777342
1102	16-17	0.24648769217603217
1102	18-19	0.2628165319558491
1102	20-21	0.21381491894664606
1102	22-23	0.21888580495914312
1102	24-25	0.23994520469508984
1102	26-27	0.24957586552040567
1102	28-29	0.2545762231498614
1102	30-31	0.3158063983238222
1102	32-33	0.2371153040762266
1102	34-35	0.3113742443231473
1102	36-37	0.2809058537137261
1102	38-39	0.29442541429731506
1102	40-41	0.28731305675778174
1102	42-43	0.3090760420033831
1102	44-45	0.30287145625404577
1102	46-47	0.363903013456671
1102	48-49	0.3277683997630234
1102	50-51	0.3360752195776655
1102	52-53	0.30920032050649127
1102	54-55	0.3471756518300353
1102	56-57	0.34654626056988747
1102	58-59	0.3675170094120972
1102	60-61	0.3319169258881942
1102	62-63	0.3267400042333648
1102	64-65	0.4401458920158987
1102	66-67	0.3645497602418004
1102	68-69	0.40437647737181237
1102	70-71	0.394542095395245
1102	72-73	0.33093185101100175
1102	74-75	0.38192026134066026
1102	76-77	0.2858063519438616
1102	78-79	0.2977917856350558
1102	80-81	0.3359095005459949
1102	82-83	0.34271596190544074
1102	84-85	0.257609603081022
1102	86-87	0.3270154497414097
1102	88-89	0.31114919062279967
1102	90-91	0.27553965375231115
1102	92-93	0.3110228883883366
1102	94-95	0.34619163107126383
1102	96-97	0.35081633028669756
1102	98-99	0.358940373717914
1102	100	0.3772884312585134
1103	1	0.04494528448459789
1103	2	0.11815605769878346
1103	3	0.0547332558551048
1103	4	0.07931099052932211
1103	5	0.11047008845122264
1103	6	0.114068017825673
1103	7	0.09635927114501186
1103	8	0.10787684851757717
1103	9	0.1915254190617901
1103	10-11	0.1752442179164433
1103	12-13	0.1776507958815614
1103	14-15	0.2422166445605285
1103	16-17	0.24441954282352896
1103	18-19	0.24916196878171348
1103	20-21	0.275404659328224
1103	22-23	0.24536588416106042
1103	24-25	0.1870220632353039
1103	26-27	0.14594839257063796
1103	28-29	0.2140362148946977
1103	30-31	0.2810865459767413
1103	32-33	0.25970363913793193
1103	34-35	0.23418988879807046
1103	36-37	0.25760630785811856
1103	38-39	0.2565890772955015
1103	40-41	0.25948679652943696
1103	42-43	0.261873756154678
1103	44-45	0.26550894716945805
1103	46-47	0.24081257195982886
1103	48-49	0.2757079572169019
1103	50-51	0.31280413980719146
1103	52-53	0.24320396394472255
1103	54-55	0.24635297093606567
1103	56-57	0.29928042253109766
1103	58-59	0.2859193625201968
1103	60-61	0.2980805703503151
1103	62-63	0.2649743919924177
1103	64-65	0.26088541001446686
1103	66-67	0.25174727084955606
1103	68-69	0.3020361569385841
1103	70-71	0.3631975153102758
1103	72-73	0.30979749296481174
1103	74-75	0.3509131919318591
1103	76-77	0.36295231135409267
1103	78-79	0.3679899790978496
1103	80-81	0.30815550886714504
1103	82-83	0.2818129382444994
1103	84-85	0.31516664806748196
1103	86-87	0.24213967937798486
1103	88-89	0.26825620666322436
1103	90-91	0.2563805992809165
1103	92-93	0.24603647101395865
1103	94-95	0.2752758274620959
1103	96-97	0.3229584594638055
1103	98-99	0.3929086331503129
1103	100	0.4169640855950618
1104	1	0.054429844631336266
1104	2	0.060028901883939056
1104	3	0.09055491407594474
1104	4	0.049826461926286925
1104	5	0.04159693986934343
1104	6	0.06997497021518484
1104	7	0.047930226335601844
1104	8	0.07639339555475289
1104	9	0.06772238424227339
1104	10-11	0.09370027990612328
1104	12-13	0.1425677334297646
1104	14-15	0.17783421503310137
1104	16-17	0.1589927328853733
1104	18-19	0.1999269824007115
1104	20-21	0.17201450001675767
1104	22-23	0.16042500494346967
1104	24-25	0.21482405080392653
1104	26-27	0.21762987689104563
1104	28-29	0.18796092954248422
1104	30-31	0.14656433053641393
1104	32-33	0.1583108270011806
1104	34-35	0.13007643241276412
1104	36-37	0.1901139848064517
1104	38-39	0.16247528970131953
1104	40-41	0.16585310407106135
1104	42-43	0.17533156093386282
1104	44-45	0.18515673019926027
1104	46-47	0.2669446359746175
1104	48-49	0.19518966265867022
1104	50-51	0.20314514299153075
1104	52-53	0.2523241943399128
1104	54-55	0.2297852726326468
1104	56-57	0.19647445257016471
1104	58-59	0.21536383062242237
1104	60-61	0.26559229277836494
1104	62-63	0.29507749258055327
1104	64-65	0.26716828446580365
1104	66-67	0.3210806420353265
1104	68-69	0.33303872617305785
1104	70-71	0.32706026235659635
1104	72-73	0.39019655233162354
1104	74-75	0.31528363485274014
1104	76-77	0.3547135696954342
1104	78-79	0.3276799676001758
1104	80-81	0.3172561821674478
1104	82-83	0.24788532157617738
1104	84-85	0.24464786458186438
1104	86-87	0.26013425701570725
1104	88-89	0.30008869068239363
1104	90-91	0.2603133102570183
1104	92-93	0.3135876230687984
1104	94-95	0.38006873517626616
1104	96-97	0.4670773729094648
1104	98-99	0.4065296765844124
1104	100	0.4171524014926149
1105	1	0.04171343605376876
1105	2	-0.020603260828295333
1105	3	0.06436141327686329
1105	4	0.061269195894936956
1105	5	0.06545314455577511
1105	6	0.07730224175587352
1105	7	0.05401630257698997
1105	8	0.05764919136613855
1105	9	0.11091106322982824
1105	10-11	0.1349462712156253
1105	12-13	0.09970733830530065
1105	14-15	0.1667420754813449
1105	16-17	0.1453887588748941
1105	18-19	0.14990732786521477
1105	20-21	0.1581509950742941
1105	22-23	0.19699154730847823
1105	24-25	0.19581894973104852
1105	26-27	0.14762785831712932
1105	28-29	0.15774272625539965
1105	30-31	0.10197297033631969
1105	32-33	0.1294584242152439
1105	34-35	0.14698798396759116
1105	36-37	0.1338563297822546
1105	38-39	0.15194986857065373
1105	40-41	0.09164638085397314
1105	42-43	0.07652434081369819
1105	44-45	0.026297391411226556
1105	46-47	-0.03990637353999915
1105	48-49	-0.08721049761306432
1105	50-51	-0.0509283543471426
1105	52-53	-0.04897922767408147
1105	54-55	-0.06803390867627712
1105	56-57	-0.0980200009047465
1105	58-59	-0.04200570955251237
1105	60-61	-0.09391359675252176
1105	62-63	-0.03120160789433868
1105	64-65	-0.05774182828596963
1105	66-67	-0.037934415311902114
1105	68-69	-0.06997319859029005
1105	70-71	-0.06439206363246086
1105	72-73	-0.0663848333814201
1105	74-75	-0.03486821350834646
1105	76-77	-0.08061511636471863
1105	78-79	-0.06250020150471869
1105	80-81	-0.05889929962668816
1105	82-83	0.01834159516731404
1105	84-85	-0.07726261697701631
1105	86-87	-0.10434552006088893
1105	88-89	-0.09212720262762986
1105	90-91	-0.1304188553388954
1105	92-93	-0.039770874140863555
1105	94-95	-0.1034935469353826
1105	96-97	-0.06691252815005555
1105	98-99	-0.08021345305508021
1105	100	-0.06390775959868833
1106	1	0.01715123676552821
1106	2	0.07221466406633681
1106	3	0.11498747945940124
1106	4	0.0410770497702444
1106	5	0.01859963634637296
1106	6	0.07450926065573782
1106	7	0.03946577200690626
1106	8	0.06599807017969539
1106	9	0.08341441434986052
1106	10-11	0.09833560719619072
1106	12-13	0.09300227666191319
1106	14-15	0.08562507374159622
1106	16-17	0.04343191104585031
1106	18-19	0.08721304584846479
1106	20-21	0.09253520384363867
1106	22-23	0.1137308698183972
1106	24-25	0.07072465767333824
1106	26-27	0.03977360355495563
1106	28-29	0.0886252493216162
1106	30-31	0.0632851810811701
1106	32-33	0.10005744792697868
1106	34-35	0.0777932539510644
1106	36-37	0.091355750627379
1106	38-39	0.1410139357975595
1106	40-41	0.12292959834173445
1106	42-43	0.020892148383858
1106	44-45	0.05147435594300731
1106	46-47	0.03685181273275617
1106	48-49	0.1799865591417742
1106	50-51	0.12985986449937315
1106	52-53	0.19090146982591705
1106	54-55	0.2342049615528552
1106	56-57	0.02024459323879313
1106	58-59	0.07848054314465713
1106	60-61	0.09976299518394427
1106	62-63	0.08473313446127406
1106	64-65	0.1023620722455334
1106	66-67	0.05684319845712338
1106	68-69	0.08156139887201164
1106	70-71	0.09305472976623008
1106	72-73	0.10409535290553862
1106	74-75	0.10412779025001129
1106	76-77	0.00870365252470151
1106	78-79	0.14689846302882614
1106	80-81	0.12373131767701295
1106	82-83	0.07893934138159864
1106	84-85	0.045449526653001726
1106	86-87	-0.0031895162169064406
1106	88-89	0.06628019490963055
1106	90-91	0.13928727257216877
1106	92-93	0.173309327888596
1106	94-95	0.1543214439961389
1106	96-97	0.11334182713842722
1106	98-99	0.15436134899362486
1106	100	0.12468034496384206
1107	1	0.008008520514714235
1107	2	0.03995899103990297
1107	3	6.500923224095345E-4
1107	4	0.02917003313977773
1107	5	0.033574212299072315
1107	6	0.016159330609518463
1107	7	0.0290385881438624
1107	8	0.03834042565307527
1107	9	0.05057982634326663
1107	10-11	0.09575702028028843
1107	12-13	0.11011024943463354
1107	14-15	0.07767842120463087
1107	16-17	0.00953932047685413
1107	18-19	0.04216282021803153
1107	20-21	0.046250913878481015
1107	22-23	0.16551121522586243
1107	24-25	0.13761708066659395
1107	26-27	0.16214632688646446
1107	28-29	0.12671562626820077
1107	30-31	0.07027510387603542
1107	32-33	0.018104236529481454
1107	34-35	0.08893638816206817
1107	36-37	0.1237078473151243
1107	38-39	0.12215872711540499
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2216	34-35	-0.2927062176135493
2216	36-37	-0.28001158090690836
2216	38-39	-0.28708044548362466
2216	40-41	-0.27388036088451173
2216	42-43	-0.2513893442710966
2216	44-45	-0.3190066578336115
2216	46-47	-0.1966403136258279
2216	48-49	-0.18503278370288
2216	50-51	-0.27506124164109025
2216	52-53	-0.19111527929828043
2216	54-55	-0.21143291667129915
2216	56-57	-0.23362630680116325
2216	58-59	-0.24739518144772177
2216	60-61	-0.3105016979031632
2216	62-63	-0.3948411257673925
2216	64-65	-0.37241893355182043
2216	66-67	-0.35892684517203577
2216	68-69	-0.3607489185041075
2216	70-71	-0.4244699654548043
2216	72-73	-0.34394463636984085
2216	74-75	-0.4325144346876826
2216	76-77	-0.39552908894673067
2216	78-79	-0.47987936971043865
2216	80-81	-0.3532241913345118
2216	82-83	-0.35124864220863117
2216	84-85	-0.4326137171423454
2216	86-87	-0.4045952735793179
2216	88-89	-0.5219642348127422
2216	90-91	-0.5056754939040289
2216	92-93	-0.48758644575946164
2216	94-95	-0.3579753789408642
2216	96-97	-0.42221532444044385
2216	98-99	-0.48873351226648154
2216	100	-0.5074278141580315
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	9.0
3	4.0
4	11.0
5	21.0
6	65.0
7	153.0
8	306.0
9	624.0
10	1095.0
11	1843.0
12	2772.0
13	4402.0
14	5290.0
15	6688.0
16	8268.0
17	12062.0
18	13885.0
19	6960.0
20	4671.0
21	4318.0
22	4462.0
23	4867.0
24	5293.0
25	6466.0
26	8193.0
27	10689.0
28	13664.0
29	17674.0
30	23007.0
31	29247.0
32	37773.0
33	49243.0
34	68544.0
35	103735.0
36	185029.0
37	410684.0
38	745354.0
39	381222.0
40	1659.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.54671965545358	20.294962348502683	13.816683942626376	23.34163405341736
2	15.582458900547994	20.66855200551349	40.26681943091334	23.482169663025182
3	17.803150530867754	26.00389688304397	30.64387552724424	25.549077058844034
4	12.10716135160759	17.39115278537778	39.535792539087204	30.965893323927425
5	12.077651645453503	37.83887168902649	36.08641210870313	13.997064556816879
6	29.740011228080476	39.13201617430678	17.762612326671096	13.365360270941654
7	26.169727169152925	33.18873231167773	23.085037876355578	17.55650264281377
8	24.76540502062381	36.162645791967606	20.713983323907794	18.357965863500787
9	24.733287711695713	16.52088841106441	21.937097179592083	36.80872669764779
10-11	23.373291499989566	26.927535680156094	29.229095807637556	20.470077012216784
12-13	24.575072055890786	24.363307544265524	29.527206029394765	21.53441437044892
14-15	21.690788207830913	26.167879872133547	27.241315018253697	24.900016901781843
16-17	20.567641976426987	29.64463896874093	27.60383855502644	22.183880499805642
18-19	20.32786779978429	27.880160817268383	30.797685666040664	20.99428571690666
20-21	21.67330733938432	27.29291260501658	30.42739115817399	20.606388897425113
22-23	21.199354385372672	27.182939738965477	30.498479989432393	21.119225886229458
24-25	20.981629618825426	27.243952047991453	30.47337162760756	21.30104670557556
26-27	20.77692537729653	27.150259970425385	30.640482183678188	21.432332468599903
28-29	20.845701956841605	27.288409391959078	30.57129280705681	21.2945958441425
30-31	21.136461936228386	27.682778434940232	30.14125909471808	21.039500534113305
32-33	20.698226627013756	27.380917435232256	30.70495979363853	21.21589614411545
34-35	20.877127965024226	27.13690894447064	30.68129280468496	21.304670285820173
36-37	21.319232822627843	27.618894513111332	29.94032341215603	21.121549252104803
38-39	21.177574885838883	27.749567481190248	29.968989823194754	21.103867809776116
40-41	20.960128866014845	27.47371974485483	30.180520665300154	21.385630723830168
42-43	21.033486037393843	27.44889122908728	29.980823317671536	21.536799415847344
44-45	21.262060532452214	27.705146010644643	29.78951515696351	21.243278299939643
46-47	21.327305283976347	28.04437285231248	29.272212569923113	21.35610929378806
48-49	21.326571423853757	27.889115570126755	29.112620926388328	21.67169207963116
50-51	21.418579136723643	27.8805615130728	28.949130650952277	21.75172869925128
52-53	21.425138011569306	27.952869668276882	28.87959740433675	21.74239491581707
54-55	21.565947422591517	28.354222355947844	28.629970297011536	21.4498599244491
56-57	21.312765680297506	28.009537429618227	28.794928292692774	21.882768597391493
58-59	21.143450390138387	27.65194115175677	29.18059472024335	22.024013737861495
60-61	21.49178168395213	28.047422958447005	28.759152611716445	21.70164274588442
62-63	21.291896533061315	27.987131762750362	28.90216360310643	21.818808101081892
64-65	21.298570073551133	27.668567670159227	29.103470607984764	21.929391648304875
66-67	21.185170609904407	27.8364216238356	28.896459880889893	22.081947885370106
68-69	21.68940420375353	27.92066201825302	28.739401405898978	21.650532372094474
70-71	21.666886060366444	27.703324066816158	28.724628494609107	21.905161378208287
72-73	21.36957889259414	27.902714357757485	28.895311730660783	21.832395018987594
74-75	21.495161212275768	27.963856380459735	28.715904371363514	21.825078035900987
76-77	21.51914090664749	27.97520653575825	28.509823635066038	21.995828922528222
78-79	21.65059360110666	28.07097528175642	28.56438154855494	21.71404956858198
80-81	21.479581259414047	27.863361666449567	28.716267660802515	21.94078941333387
82-83	21.314455970146515	27.92544701336729	28.87033952100862	21.88975749547758
84-85	21.351408002377706	27.876915145588676	28.67753360620699	22.09414324582663
86-87	21.463631549725502	27.96517989234644	28.571146819298566	22.00004173862949
88-89	21.132792128417755	27.91117041258198	28.644269302269475	22.31176815673079
90-91	21.099450096069212	27.80615439232841	28.707150127121423	22.38724538448096
92-93	21.276865577683452	27.967101628521217	28.50521011664139	22.25082267715394
94-95	20.98158406150541	27.74939903157051	28.7173469233306	22.55166998359348
96-97	20.915159037245374	27.69896396917042	28.812463192193167	22.573413801391037
98-99	20.99401558767984	27.764691852645168	28.64248210352265	22.59881045615234
100	21.099716913457712	27.74683843885936	28.490926736909312	22.662517910773616
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	57.0
1	41.0
2	137.0
3	262.5
4	170.5
5	103.5
6	290.0
7	696.5
8	1204.5
9	1402.5
10	1102.0
11	830.0
12	856.0
13	1018.0
14	1200.0
15	1403.5
16	1689.0
17	2006.5
18	2316.5
19	2683.0
20	3244.5
21	4065.0
22	5279.0
23	7196.0
24	9635.0
25	12579.0
26	16589.5
27	21502.0
28	27353.0
29	34122.5
30	40540.0
31	46601.0
32	52604.0
33	58622.5
34	64430.0
35	68870.0
36	72206.0
37	75297.0
38	77508.0
39	77624.5
40	77862.0
41	78405.0
42	76734.5
43	75160.0
44	73451.5
45	71685.5
46	70913.0
47	69084.0
48	67219.5
49	65507.5
50	63369.0
51	61154.0
52	57261.5
53	53921.0
54	52002.0
55	49758.0
56	46285.0
57	41576.0
58	37647.5
59	34467.0
60	30892.5
61	26595.5
62	22496.0
63	18839.0
64	15185.0
65	11872.5
66	9328.5
67	7064.5
68	5269.5
69	4017.0
70	2896.0
71	2052.5
72	1535.0
73	1089.5
74	787.0
75	570.5
76	365.5
77	235.0
78	156.5
79	107.0
80	64.0
81	27.5
82	14.5
83	4.5
84	4.0
85	5.0
86	2.5
87	1.0
88	0.5
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.5
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.04233455582198755
2	0.23955946376840845
3	0.0022015803677740003
4	0.0028895742327033755
5	9.173251532391669E-5
6	1.8346503064783337E-4
7	0.0
8	2.293312883097917E-4
9	0.0
10-11	6.191944784364376E-4
12-13	0.0
14-15	3.8986319012664593E-4
16-17	2.0639815947881256E-4
18-19	6.191944784364376E-4
20-21	4.586625766195834E-5
22-23	1.8346503064783337E-4
24-25	0.0013989208586897295
26-27	1.8346503064783337E-4
28-29	1.8346503064783337E-4
30-31	1.3759877298587502E-4
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	1.3759877298587502E-4
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	2.293312883097917E-5
68-69	4.586625766195834E-4
70-71	4.586625766195834E-5
72-73	5.733282207744793E-4
74-75	2.5226441714077085E-4
76-77	0.0
78-79	0.0
80-81	0.0
82-83	1.8346503064783337E-4
84-85	0.0
86-87	8.02659509084271E-4
88-89	0.002522644171407709
90-91	0.002430911656083792
92-93	0.0010319907973940627
94-95	9.631914109011252E-4
96-97	3.6693006129566674E-4
98-99	2.0639815947881256E-4
100	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	2180252.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	71.0936389485552
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.50618716895039	57.94571442662721
2	12.124408454850709	17.239366343079325
3	3.248341494218512	6.928092521147436
4	1.207555444877286	3.433980432338709
5	0.5694199047627748	2.0241066559662695
6	0.33710500619982076	1.4379612959112311
7	0.22954947305860823	1.1423655150921852
8	0.1456354341094639	0.828300237655546
9	0.1096681864849773	0.7017039408695217
>10	0.4967505031133415	6.000310893444011
>50	0.01904211964312046	0.9212530776156558
>100	0.0058203276647163195	0.7348084906519615
>500	2.582410331913194E-4	0.10942139686141582
>1k	2.5824103319125366E-4	0.5526147727395654
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4827	0.22139642573427293	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3984	0.18273117052524204	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	4.586625766195834E-5	0.0
5	0.0	0.0	0.0	4.586625766195834E-5	0.0
6	0.0	0.0	0.0	4.586625766195834E-5	0.0
7	0.0	0.0	0.0	9.173251532391669E-5	0.0
8	0.0	0.0	0.0	9.173251532391669E-5	0.0
9	0.0	0.0	0.0	1.3759877298587502E-4	0.0
10-11	0.0	0.0	0.0	1.8346503064783337E-4	0.0
12-13	0.0	0.0	0.0	1.8346503064783337E-4	0.0
14-15	0.0	0.0	0.0	2.0639815947881253E-4	0.0
16-17	0.0	0.0	0.0	2.522644171407709E-4	0.0
18-19	0.0	0.0	0.0	2.7519754597175004E-4	0.0
20-21	0.0	0.0	0.0	2.7519754597175004E-4	0.0
22-23	0.0	0.0	0.0	2.7519754597175004E-4	0.0
24-25	0.0	0.0	0.0	3.210638036337084E-4	0.0
26-27	0.0	0.0	0.0	4.5866257661958344E-4	0.0
28-29	0.0	0.0	0.0	0.0010778570550560211	0.0
30-31	0.0	0.0	0.0	0.0031418386498441465	0.0
32-33	0.0	0.0	0.0	0.007934862575518793	0.0
34-35	0.0	0.0	0.0	0.015571594476234856	0.0
36-37	0.0	0.0	0.0	0.0271986907935413	0.0
38-39	0.0	0.0	0.0	0.04848063434868997	0.0
40-41	0.0	0.0	0.0	0.07639025213599163	0.0
42-43	0.0	0.0	0.0	0.10696011286768686	0.0
44-45	0.0	0.0	0.0	0.13931875764819845	0.0
46-47	0.0	0.0	0.0	0.1806901220592849	0.0
48-49	0.0	0.0	0.0	0.22866622757369331	0.0
50-51	0.0	0.0	0.0	0.2838433355410292	0.0
52-53	0.0	0.0	0.0	0.33982310301744934	0.0
54-55	0.0	0.0	0.0	0.4019948152782339	0.0
56-57	0.0	0.0	0.0	0.47148219563610083	0.0
58-59	0.0	0.0	0.0	0.5550734502250199	0.0
60-61	0.0	0.0	0.0	0.6456134428497257	0.0
62-63	0.0	0.0	0.0	0.7400520673756978	0.0
64-65	0.0	0.0	0.0	0.8425860863790058	0.0
66-67	0.0	0.0	0.0	0.955646411515733	0.0
68-69	0.0	0.0	0.0	1.084461796159343	0.0
70-71	0.0	0.0	0.0	1.228734109635033	0.0
72-73	0.0	0.0	0.0	1.3872249629859301	0.0
74-75	0.0	0.0	0.0	1.5455782175638413	0.0
76-77	0.0	0.0	0.0	1.7085410310367792	0.0
78-79	0.0	0.0	0.0	1.9026699665910178	0.0
80-81	0.0	0.0	0.0	2.11491607392173	0.0
82-83	0.0	0.0	0.0	2.3479395959733096	0.0
84-85	0.0	0.0	0.0	2.5738538480872855	0.0
86-87	0.0	0.0	0.0	2.8117391934510323	0.0
88	0.0	0.0	0.0	3.0118536756301566	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	3250	0.0	57.1348	1
GTATCAA	6000	0.0	42.337517	1
ATCAACG	7350	0.0	34.210342	3
TCAACGC	7360	0.0	34.16308	4
CAACGCA	7555	0.0	33.156128	5
TATCAAC	7630	0.0	33.03219	2
AACGCAG	7810	0.0	32.193916	6
ACGCAGA	9170	0.0	27.265491	7
GTACATG	5740	0.0	26.848728	1
CGCAGAG	9295	0.0	26.7977	8
TACATGG	5765	0.0	25.005194	2
GCAGAGT	10215	0.0	24.062147	9
ATAACGC	215	1.880835E-9	24.046131	3
ACATGGG	6055	0.0	23.28617	3
TAACGCA	205	2.9906005E-8	22.925941	4
TATAACG	185	2.7546594E-7	22.918114	2
TGGTATC	1475	0.0	22.35703	2
GTGGTAT	1520	0.0	22.014185	1
CGTACAC	150	3.293522E-5	21.932985	3
GAGTACT	7610	0.0	20.565327	12-13
>>END_MODULE