FastQCFastQC Report
Wed 25 May 2016
SRR1033083_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033083_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2673909
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC29250.033.5878034
GTATCAA31850.032.4718441
GGTATCA20600.031.7205921
CAACGCA31150.031.5385195
ATCAACG31250.031.2877623
AACGCAG33900.028.9757426
GTACATG68900.027.6330931
TATCAAC37400.026.6455972
TACATGG68150.026.1417432
ACATGGG69550.024.9396533
ACGCAGA39600.024.2115737
TACACCG2751.8189894E-1223.9303155
CGCAGAG40800.023.1760168
CATGGGG49900.021.6664874
TATACCG1951.1310702E-519.2845025
CCGCGCA1250.00637269418.8207159
GGACGCG2552.9586408E-718.4516839
GCAGAGT51300.018.2520589
GAGTACA47650.018.153011
CCGGTCG1300.00799074518.0968429
Produced by FastQC (version 0.11.5)