##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1033083_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2673909
Sequences flagged as poor quality	0
Sequence length	100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.604091238707078	33.0	31.0	34.0	30.0	34.0
2	31.655035006800905	34.0	31.0	34.0	30.0	34.0
3	31.836573346362947	34.0	31.0	34.0	30.0	34.0
4	35.23999358242932	37.0	35.0	37.0	33.0	37.0
5	35.26924027706253	37.0	35.0	37.0	33.0	37.0
6	35.31366400277646	37.0	35.0	37.0	33.0	37.0
7	35.27136862174442	37.0	36.0	37.0	33.0	37.0
8	35.24325659549371	37.0	36.0	37.0	33.0	37.0
9	36.894072685345684	39.0	38.0	39.0	34.0	39.0
10-11	36.89019932989492	39.0	38.0	39.0	34.0	39.0
12-13	36.81853309144029	39.0	38.0	39.0	33.5	39.0
14-15	38.22566325181597	41.0	39.0	41.0	34.0	41.0
16-17	38.23132743111303	41.0	39.0	41.0	34.0	41.0
18-19	38.23335349108739	41.0	39.0	41.0	34.0	41.0
20-21	38.266100117842456	41.0	39.0	41.0	34.0	41.0
22-23	38.23100711355548	40.0	39.0	41.0	34.0	41.0
24-25	38.24325435158788	40.0	39.0	41.0	34.0	41.0
26-27	38.16041383607296	40.0	38.0	41.0	33.0	41.0
28-29	38.17244734207485	40.0	38.0	41.0	33.0	41.0
30-31	38.31751959397272	40.5	39.0	41.0	34.0	41.0
32-33	38.30824197831714	41.0	39.0	41.0	34.0	41.0
34-35	38.26972028591848	41.0	39.0	41.0	33.5	41.0
36-37	38.19552553957521	41.0	38.0	41.0	33.0	41.0
38-39	38.11022102846432	40.5	38.0	41.0	33.0	41.0
40-41	38.045449751655724	40.0	38.0	41.0	33.0	41.0
42-43	37.92925544586596	40.0	38.0	41.0	33.0	41.0
44-45	37.79660339974173	40.0	38.0	41.0	33.0	41.0
46-47	37.67399096229528	40.0	38.0	41.0	32.0	41.0
48-49	37.56613089675079	40.0	38.0	41.0	32.0	41.0
50-51	37.45010694081212	40.0	37.5	41.0	32.0	41.0
52-53	37.30638421128019	40.0	37.0	41.0	31.0	41.0
54-55	37.159476631403685	40.0	37.0	41.0	31.0	41.0
56-57	36.99762239477858	40.0	36.5	41.0	31.0	41.0
58-59	36.812640968709104	40.0	36.0	41.0	31.0	41.0
60-61	36.595453136213685	39.0	35.5	41.0	30.5	41.0
62-63	36.353771762614215	39.0	35.0	41.0	30.0	41.0
64-65	36.08580770699377	39.0	35.0	41.0	30.0	41.0
66-67	35.794643721981565	38.0	35.0	40.0	30.0	41.0
68-69	35.482313160245916	37.5	35.0	40.0	29.5	41.0
70-71	35.145177528479834	37.0	34.5	40.0	29.0	41.0
72-73	34.79308925621628	36.5	34.0	39.0	29.0	41.0
74-75	34.43436070561863	36.0	34.0	39.0	29.0	41.0
76-77	34.06555851377141	35.5	34.0	38.5	28.5	40.0
78-79	33.67122684429425	35.0	34.0	37.0	28.0	39.5
80-81	33.31157324351726	35.0	34.0	37.0	28.0	39.0
82-83	33.078808403726526	35.0	34.0	36.5	28.0	39.0
84-85	32.84433239874655	35.0	34.0	36.0	27.5	37.5
86-87	32.659649786137074	35.0	34.0	36.0	28.0	37.0
88-89	32.435028641588026	35.0	34.0	35.5	27.0	37.0
90-91	32.22116440761447	35.0	33.5	35.0	27.0	36.0
92-93	31.999144884885762	35.0	33.0	35.0	27.0	36.0
94-95	31.800892812732222	35.0	33.0	35.0	26.0	36.0
96-97	31.631736906528978	35.0	33.0	35.0	26.0	36.0
98-99	31.464350881050926	35.0	33.0	35.0	25.0	35.0
100	31.330573703143973	35.0	33.0	35.0	25.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.07350211172514065
1101	2	0.12565200517163788
1101	3	0.13005207228435367
1101	4	0.04993186798449756
1101	5	0.04135931075560961
1101	6	0.1724905052625374
1101	7	0.1827225537423658
1101	8	0.2550190534391277
1101	9	0.22603815995678644
1101	10-11	0.22861947293151985
1101	12-13	0.16064211370297699
1101	14-15	0.23035346889026442
1101	16-17	0.23502889606465516
1101	18-19	0.29055558390862046
1101	20-21	0.2848983121324764
1101	22-23	0.2713154271291529
1101	24-25	0.2447159302625863
1101	26-27	0.15343867549523083
1101	28-29	0.19330231219690575
1101	30-31	0.2705101763394495
1101	32-33	0.24813511420063605
1101	34-35	0.27589626497270103
1101	36-37	0.23427679061094153
1101	38-39	0.29064660663543407
1101	40-41	0.2517638827417059
1101	42-43	0.287096066251614
1101	44-45	0.2634276084728384
1101	46-47	0.23726015241047804
1101	48-49	0.2685868303492356
1101	50-51	0.20860746496348526
1101	52-53	0.14096527914990276
1101	54-55	0.19316834252128245
1101	56-57	0.21262892192622473
1101	58-59	0.24614331490624863
1101	60-61	0.2672647805285209
1101	62-63	0.28041619019835906
1101	64-65	0.30640258100266493
1101	66-67	0.2780309576356572
1101	68-69	0.23692487436862564
1101	70-71	0.2451855491426116
1101	72-73	0.2628641288040612
1101	74-75	0.20500327306137223
1101	76-77	0.2186053296071364
1101	78-79	0.18583957399197715
1101	80-81	0.27745527348425014
1101	82-83	0.2774660113632379
1101	84-85	0.22296638370108468
1101	86-87	0.21134725555175748
1101	88-89	0.25862353260814075
1101	90-91	0.27735431788169507
1101	92-93	0.2778622517943532
1101	94-95	0.25778290115220415
1101	96-97	0.3032917540983746
1101	98-99	0.3341337197246901
1101	100	0.2709446650527134
1102	1	-0.08520088949568105
1102	2	0.007838779465810575
1102	3	0.07634273588034901
1102	4	-0.034690389497377794
1102	5	-0.08413862694007435
1102	6	0.10714445791619198
1102	7	0.14153808630212694
1102	8	0.15735317369592394
1102	9	0.2468902709784615
1102	10-11	0.1580041321602792
1102	12-13	0.14284463930366087
1102	14-15	0.20342539728921594
1102	16-17	0.22059342057931275
1102	18-19	0.14045791810589492
1102	20-21	0.21864962634601426
1102	22-23	0.13434160356689517
1102	24-25	0.1797790317680139
1102	26-27	0.019880882834804936
1102	28-29	0.11339293034903619
1102	30-31	0.14924753004292768
1102	32-33	0.11318554533970371
1102	34-35	0.07996899826726178
1102	36-37	0.19283179116936822
1102	38-39	0.22485659581362683
1102	40-41	0.09988755167928076
1102	42-43	0.13294215018412814
1102	44-45	0.17501575609608722
1102	46-47	0.09670334950206438
1102	48-49	0.10482126678751058
1102	50-51	0.19884616080151574
1102	52-53	0.16300667158079563
1102	54-55	0.18634340539171745
1102	56-57	0.19250583836728197
1102	58-59	0.12863040384144142
1102	60-61	0.07365769282570511
1102	62-63	0.09442880664827413
1102	64-65	0.06306580728615785
1102	66-67	0.06139364976116468
1102	68-69	0.10174223238542623
1102	70-71	0.08162583089458764
1102	72-73	0.14555086266089745
1102	74-75	0.12693956692144326
1102	76-77	0.15987702616534705
1102	78-79	0.0605519104614487
1102	80-81	0.15637347837447635
1102	82-83	0.13712418105372137
1102	84-85	0.11345594509074175
1102	86-87	0.12635593995180727
1102	88-89	0.19333259557870974
1102	90-91	0.22968481803382446
1102	92-93	0.18474233775258853
1102	94-95	0.2735209211089895
1102	96-97	0.17110806074435203
1102	98-99	0.20658006322641143
1102	100	0.17539693166186865
1103	1	0.037392344456876
1103	2	0.05918100941710591
1103	3	0.06480051624540195
1103	4	0.06304369938528964
1103	5	0.06501388545795805
1103	6	0.1201713467281138
1103	7	0.14399190224382608
1103	8	0.10945763404063769
1103	9	0.1955608627681258
1103	10-11	0.18395415684582161
1103	12-13	0.2054425156107058
1103	14-15	0.2406990088199379
1103	16-17	0.25167847882980965
1103	18-19	0.22878143863174216
1103	20-21	0.2286446892388554
1103	22-23	0.22609899264777766
1103	24-25	0.19219703526083265
1103	26-27	0.16480524193197965
1103	28-29	0.19586251555834622
1103	30-31	0.2251641003009084
1103	32-33	0.16274953245901003
1103	34-35	0.15426722901438694
1103	36-37	0.2483838260680784
1103	38-39	0.2789525525439345
1103	40-41	0.3002388231685913
1103	42-43	0.2416515019493275
1103	44-45	0.2034672729669893
1103	46-47	0.24481796407611256
1103	48-49	0.23455930535590142
1103	50-51	0.24915351414364295
1103	52-53	0.2309362551689702
1103	54-55	0.2691047220518712
1103	56-57	0.17161273779029784
1103	58-59	0.1641867126538088
1103	60-61	0.12501664999077633
1103	62-63	0.18524423989784822
1103	64-65	0.19375427582500038
1103	66-67	0.1788276804301816
1103	68-69	0.16425868326857795
1103	70-71	0.1711216564599667
1103	72-73	0.10133914182114978
1103	74-75	0.03678172686897341
1103	76-77	0.08649392875695128
1103	78-79	0.038258218905376395
1103	80-81	0.05336029556150379
1103	82-83	0.02756903651437881
1103	84-85	-0.024563243307788696
1103	86-87	-0.054368635788577535
1103	88-89	0.03968075742739785
1103	90-91	-0.004028844489113226
1103	92-93	0.03179569535388538
1103	94-95	0.06371523680383717
1103	96-97	0.020249225164754137
1103	98-99	-0.03868900911325923
1103	100	0.04133045019812798
1104	1	-0.04445172431773514
1104	2	-0.050196180617291475
1104	3	0.025186062515935248
1104	4	-0.012813199100435213
1104	5	-0.06940420639105582
1104	6	-0.01644565433996803
1104	7	0.06350873363518872
1104	8	0.03416420736558479
1104	9	0.06093598579821702
1104	10-11	0.0457772690423397
1104	12-13	0.04285337393268662
1104	14-15	0.022449311481366863
1104	16-17	0.0037808412757129872
1104	18-19	0.03969419521290263
1104	20-21	-0.0064841788250404875
1104	22-23	0.029234829017376285
1104	24-25	-0.0563297089716599
1104	26-27	-0.05798222432076017
1104	28-29	-0.06559309783901313
1104	30-31	-0.0050619490065386685
1104	32-33	-0.003381421946770047
1104	34-35	0.08359732003230391
1104	36-37	0.018762676170602788
1104	38-39	0.030711672861684747
1104	40-41	0.12027115645708619
1104	42-43	0.1364467133987617
1104	44-45	0.04284618926898531
1104	46-47	-0.023021240096177564
1104	48-49	0.02501332079124552
1104	50-51	-0.003509708833249192
1104	52-53	0.042425045933342176
1104	54-55	0.013273416930047688
1104	56-57	-5.559186950137018E-4
1104	58-59	0.01322891643836499
1104	60-61	0.05510408269210387
1104	62-63	0.09301045386271056
1104	64-65	-0.013987243903073932
1104	66-67	0.019289430899398496
1104	68-69	0.08287356454216166
1104	70-71	-0.021768668805272284
1104	72-73	0.04933924740154083
1104	74-75	0.09469620505978327
1104	76-77	-0.031210596901267706
1104	78-79	0.031547406556022395
1104	80-81	-0.0017716171133557168
1104	82-83	0.03465188564353383
1104	84-85	-0.01700429437467932
1104	86-87	-0.055419993449348226
1104	88-89	-0.03417131343736912
1104	90-91	-0.004376532850251635
1104	92-93	0.023266832456094733
1104	94-95	0.03865411521545781
1104	96-97	0.036216812267518605
1104	98-99	0.03533399470810039
1104	100	0.0010798322776075509
1105	1	-0.10489243425491424
1105	2	-0.11343973603214152
1105	3	-0.05718543951247668
1105	4	-0.07717782571673126
1105	5	-0.09711151295502418
1105	6	-0.030228566588867523
1105	7	0.05795790717426286
1105	8	0.028111896649257062
1105	9	0.05680252560441801
1105	10-11	0.07969444589632957
1105	12-13	0.025756183992953652
1105	14-15	0.07401677802860007
1105	16-17	0.07129069729024451
1105	18-19	0.08620830017773073
1105	20-21	0.12066857431079825
1105	22-23	0.16972323823978286
1105	24-25	0.10329465212326738
1105	26-27	0.07652980691354827
1105	28-29	0.10855749226914924
1105	30-31	0.10011529647026407
1105	32-33	0.07741187174970321
1105	34-35	0.07521896242071335
1105	36-37	0.13239327413262458
1105	38-39	0.14414274225642032
1105	40-41	0.2165712731463003
1105	42-43	0.10836386305496859
1105	44-45	0.1238755208727369
1105	46-47	0.09703285803704631
1105	48-49	0.08555655833367126
1105	50-51	0.023743709596544704
1105	52-53	0.11278480762984344
1105	54-55	0.049688536030551234
1105	56-57	0.022824260699159993
1105	58-59	0.0017812286619474094
1105	60-61	-0.01410513237249944
1105	62-63	-0.015617426665592404
1105	64-65	0.0348525395141408
1105	66-67	0.04694857193042168
1105	68-69	0.0037848215908908855
1105	70-71	0.025661418655701596
1105	72-73	0.04607782451666509
1105	74-75	0.046180249439863985
1105	76-77	0.0117938551615282
1105	78-79	-0.007791139328539032
1105	80-81	0.022859291881459853
1105	82-83	0.05184714837915294
1105	84-85	0.01666076706450781
1105	86-87	-0.033884004652151134
1105	88-89	0.05858210509290984
1105	90-91	0.06875375183484778
1105	92-93	0.07662181638628596
1105	94-95	0.06886489066914692
1105	96-97	0.11128785307913347
1105	98-99	0.07508094277849153
1105	100	0.018349219021846608
1106	1	-0.06628587552444287
1106	2	-0.01750709390704941
1106	3	-0.027718464964738843
1106	4	0.017654052675325715
1106	5	0.03422230811069227
1106	6	-0.041829167109362686
1106	7	0.02359696996600036
1106	8	0.08999884942685554
1106	9	0.09191582812423604
1106	10-11	0.05673990534912576
1106	12-13	0.02972053380376849
1106	14-15	0.14018577349039418
1106	16-17	0.1386446030232591
1106	18-19	0.14610298046533643
1106	20-21	0.09139548608839476
1106	22-23	0.07959683426273045
1106	24-25	0.06758248027888669
1106	26-27	0.049027955689702196
1106	28-29	0.04042727915296496
1106	30-31	0.07479036381925397
1106	32-33	0.00692711148159475
1106	34-35	0.06457575898422618
1106	36-37	0.03341824247448244
1106	38-39	0.0717897383202768
1106	40-41	0.07301540556056096
1106	42-43	0.029931859173437658
1106	44-45	0.06073646760702189
1106	46-47	0.08856152438109888
1106	48-49	0.08177996643993879
1106	50-51	0.03454199669056379
1106	52-53	-0.01681476916069613
1106	54-55	0.0025507249582616964
1106	56-57	0.04427711895066011
1106	58-59	0.0337203649945792
1106	60-61	0.07744560259194344
1106	62-63	0.013137318881625504
1106	64-65	0.06845393514004883
1106	66-67	0.08414496169464059
1106	68-69	0.1310566193546947
1106	70-71	0.004112030035869907
1106	72-73	0.05284419054203937
1106	74-75	0.09422982794509238
1106	76-77	0.08747363217638338
1106	78-79	-0.011102829537186665
1106	80-81	0.054697611135324564
1106	82-83	0.06466796896349081
1106	84-85	0.11827568202252081
1106	86-87	0.12781712716640925
1106	88-89	0.1488941712619365
1106	90-91	0.123149344715479
1106	92-93	0.14582084930441397
1106	94-95	0.15739095450366136
1106	96-97	0.13292227733263928
1106	98-99	0.18210006595251116
1106	100	0.1723171252146507
1107	1	0.06502562593631112
1107	2	0.0673487252771281
1107	3	0.08676379205483187
1107	4	0.1644917316025527
1107	5	0.11043644626513327
1107	6	0.16792733294563789
1107	7	0.2416962768712736
1107	8	0.16216687905757254
1107	9	0.2812957100414337
1107	10-11	0.21761000204012504
1107	12-13	0.21388937021140464
1107	14-15	0.36056147483625267
1107	16-17	0.24460405637076832
1107	18-19	0.2780806840848271
1107	20-21	0.2401046430065037
1107	22-23	0.20650039997226344
1107	24-25	0.12785482861093556
1107	26-27	0.10855006259987476
1107	28-29	0.10760887730470614
1107	30-31	0.20784804836947757
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2216	50-51	-0.5522986175342979
2216	52-53	-0.6057134547825314
2216	54-55	-0.616032970450199
2216	56-57	-0.7189793756772929
2216	58-59	-0.610356297975585
2216	60-61	-0.5973630581239675
2216	62-63	-0.6156041746252612
2216	64-65	-0.6721266576329938
2216	66-67	-0.7210907355802405
2216	68-69	-0.6536238152566654
2216	70-71	-0.6129860968691858
2216	72-73	-0.6332931798532471
2216	74-75	-0.6570336476125647
2216	76-77	-0.5372873073404065
2216	78-79	-0.5032005019546375
2216	80-81	-0.5179949552653085
2216	82-83	-0.7205936038060372
2216	84-85	-0.5526083266793975
2216	86-87	-0.5875503109172229
2216	88-89	-0.6273611235187282
2216	90-91	-0.6266384095703366
2216	92-93	-0.8438591005201523
2216	94-95	-0.6979969961207999
2216	96-97	-0.7067538850951713
2216	98-99	-0.6626662062189119
2216	100	-0.6890256735806517
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	27898.0
3	5710.0
4	3189.0
5	2562.0
6	2168.0
7	2345.0
8	2753.0
9	3126.0
10	3558.0
11	3959.0
12	4364.0
13	4885.0
14	5361.0
15	5560.0
16	5496.0
17	5582.0
18	5696.0
19	5663.0
20	5610.0
21	5723.0
22	6776.0
23	9493.0
24	14937.0
25	20676.0
26	21096.0
27	18157.0
28	18873.0
29	23660.0
30	28952.0
31	35460.0
32	46409.0
33	62489.0
34	89285.0
35	136877.0
36	246100.0
37	523865.0
38	881205.0
39	377628.0
40	763.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.62202142950428	20.069213863578785	13.550140571949898	23.758624134967036
2	15.078190094886187	20.317377293538076	40.91902778416937	23.685404827406362
3	17.81666830065237	25.563519260949512	30.383116013234535	26.23669642516358
4	11.920923154540507	16.99375196958548	39.856662831666966	31.228662044207038
5	12.515234590230698	38.23245813669357	36.20880297295355	13.043504300122176
6	30.2926705466807	38.83512735320056	18.136956909085193	12.735245191033545
7	26.745404819820124	32.7618322473802	23.11769995051258	17.375062982287098
8	24.314003559611514	37.398758104405786	20.720173652734964	17.56706468324774
9	24.448437678408645	17.416427540585918	21.538081413305328	36.597053367700106
10-11	23.289552895933294	27.492778137186193	29.361963957326243	19.855705009554274
12-13	23.926672882965537	24.770646838907194	29.350652608421278	21.95202766970599
14-15	21.29759539201821	26.853043830675016	26.296870013019387	25.552490764287395
16-17	21.038787612037048	30.178555655859313	26.86918668624835	21.913470045855284
18-19	20.960917667556277	29.235603979773938	28.701631884013484	21.101846468656305
20-21	22.252849424780706	28.46862427460042	28.177481800085914	21.101044500532964
22-23	21.77053129747169	28.271089895227604	28.24416568920635	21.714213118094357
24-25	21.65404992109001	28.401087944146912	28.1686597720644	21.776202362698672
26-27	21.418465996478837	28.311751616614895	28.294067110452854	21.975715276453418
28-29	21.483279865202302	28.32581598542748	28.365035303935688	21.82586884543453
30-31	21.71352657176601	28.845429402485916	27.95112978591491	21.489914239833162
32-33	21.340052432929077	28.477742202894103	28.462472656096498	21.719732708080322
34-35	21.463286083194884	28.231466763026052	28.52546388384056	21.77978326993851
36-37	21.841540433805488	28.5751196845432	27.94934370886253	21.633996172788784
38-39	21.69095135078487	28.70964300952923	28.117809101048742	21.481596538637163
40-41	21.394147405402382	28.45447431959316	28.443544615370797	21.707833659633664
42-43	21.398103711078402	28.255528347488912	28.382315650793146	21.96405229063954
44-45	21.590708345497145	28.46045451483236	28.318680619493534	21.630156520176953
46-47	21.537661122417948	28.681198680609164	28.195101410625306	21.58603878634758
48-49	21.50117974138512	28.472545561522466	28.200460218910894	21.82581447818152
50-51	21.507416889463094	28.37297759260843	28.249077391684636	21.870528126243844
52-53	21.552354441039554	28.289335423050833	28.31473016912676	21.84357996678285
54-55	21.602341881986003	28.788651662644142	28.088306345828034	21.520700109541817
56-57	21.471374226858334	28.444277942335837	28.117515474996647	21.966832355809185
58-59	21.34188204196724	28.07795706721461	28.55595896874341	22.02420192207474
60-61	21.579233262048355	28.53184818077356	28.120660661863152	21.76825789531493
62-63	21.47725820356453	28.50630257486676	28.144359007898125	21.872080213670582
64-65	21.41478726639066	28.26601174186607	28.310060112740626	22.009140879002643
66-67	21.285053763621946	28.33269804629119	28.192317386786513	22.18993080330035
68-69	21.74626069135762	28.448854012210994	28.138302212036	21.666583084395388
70-71	21.67310411489624	28.242276325103017	28.226409572890603	21.858209987110136
72-73	21.470692738853835	28.382981916129502	28.358957653869883	21.787367691146777
74-75	21.482064708839786	28.44164137725343	28.166010317594115	21.91028359631267
76-77	21.48625433089517	28.410017478873613	28.072564080783753	22.03116410944746
78-79	21.64068845093675	28.486375959858762	28.16710125320701	21.70583433599748
80-81	21.560769253219128	28.213122863050387	28.326027138932687	21.900080744797798
82-83	21.372691285717973	28.330088470077218	28.44584458558113	21.851375658623674
84-85	21.385167228703974	28.24309494794997	28.325483019713648	22.046254803632408
86-87	21.568963078864016	28.332936742221015	28.15200140078974	21.94609877812523
88-89	21.267627239767876	28.244478450711075	28.281987196515896	22.205907113005154
90-91	21.301710725420982	28.20591485339325	28.383779863428554	22.10859455775721
92-93	21.5160524165427	28.366540154986787	28.151760886938664	21.96564654153185
94-95	21.310902235595744	28.038753693489266	28.389867843469947	22.260476227445043
96-97	21.269097534342215	28.11082598478801	28.40580919368076	22.214267287189017
98-99	21.286197586553183	28.18096885088143	28.309921232974094	22.22291232959129
100	21.40693540523136	28.09575144408094	28.202170209406237	22.295142941281462
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	474.0
1	429.5
2	365.5
3	336.0
4	336.0
5	332.5
6	318.0
7	306.5
8	286.0
9	253.0
10	241.5
11	236.5
12	228.5
13	261.0
14	294.5
15	331.5
16	450.0
17	635.5
18	873.5
19	1199.5
20	1679.0
21	2573.5
22	4012.0
23	6166.5
24	9069.0
25	12478.0
26	17207.5
27	23681.0
28	31373.0
29	39245.0
30	46658.0
31	54463.0
32	62533.0
33	70930.0
34	78853.0
35	84817.0
36	89557.5
37	93930.5
38	97631.5
39	98706.5
40	99261.5
41	100390.5
42	99999.0
43	98496.5
44	95961.0
45	93651.0
46	91637.5
47	88833.5
48	86678.0
49	84410.5
50	81536.0
51	78777.5
52	73220.5
53	68347.0
54	65490.5
55	61550.5
56	56135.5
57	50955.5
58	45924.0
59	40117.0
60	35082.5
61	29725.0
62	24762.0
63	20400.5
64	16350.0
65	13063.5
66	9985.0
67	7350.5
68	5716.0
69	4606.0
70	3495.5
71	2495.5
72	1856.0
73	1388.0
74	976.5
75	621.5
76	358.5
77	261.5
78	187.0
79	96.5
80	54.5
81	33.5
82	23.5
83	19.0
84	15.0
85	10.5
86	12.0
87	15.0
88	9.0
89	6.5
90	8.0
91	4.5
92	3.0
93	3.5
94	2.5
95	1.0
96	0.5
97	1.5
98	1.5
99	1.0
100	8.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.06028627002639208
2	0.042185429646259466
3	0.05635943481995835
4	0.07580661869943967
5	0.0881481007768028
6	0.09128956894194978
7	0.09435624024602184
8	0.10587495685156077
9	0.1018733247840521
10-11	0.10669772232338498
12-13	0.1112042332031494
14-15	0.10813756189907735
16-17	0.07573182183836473
18-19	0.10105055931222791
20-21	0.0948611190582776
22-23	0.05955700063091152
24-25	0.022813042627853077
26-27	0.1834767002167987
28-29	0.06569034323905563
30-31	0.07844320805233088
32-33	0.07195458035408085
34-35	0.021821984218610282
36-37	0.05486349759845978
38-39	0.07962125861426099
40-41	0.08566110514606144
42-43	0.07896678607985537
44-45	0.07662938417126386
46-47	0.08308061343897642
48-49	0.0799204460585607
50-51	0.07481556029019686
52-53	0.07840580962179342
54-55	0.0699350651050578
56-57	0.07245945916633663
58-59	0.06913099884850232
60-61	0.06621392126658013
62-63	0.07275864661063634
64-65	0.06905620198742739
66-67	0.07062693607000088
68-69	0.07072043214634455
70-71	0.061875703324234296
72-73	0.060230172380585874
74-75	0.06273586722659596
76-77	0.057593583027694656
78-79	0.0540594313419043
80-81	0.04828137382386611
82-83	0.05534967719544681
84-85	0.04457892920065716
86-87	0.042297624937871856
88-89	0.044840718214419414
90-91	0.040969980653791885
92-93	0.043569171576145635
94-95	0.042316324153140586
96-97	0.045420393887750106
98-99	0.045513889964093765
100	0.05415292741824797
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	2673909.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	68.51583778479119
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.50020957286898	54.47023462951596
2	12.809632552243306	17.55325412064566
3	3.824184340790578	7.860515818582376
4	1.5060881028254702	4.127635525711754
5	0.7249005783477306	2.4833585218087215
6	0.4450203516330429	1.829456533405218
7	0.27163608983463955	1.3027961987323593
8	0.18740942194755764	1.0272410842800277
9	0.12008335649635665	0.7404850596921861
>10	0.5811986795262658	6.845252174713902
>50	0.023261592845726934	1.0648718492765876
>100	0.006320725269356238	0.6667107703813611
>500	5.4635370964694836E-5	0.028187713253896785
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	3.739843053746406E-5	0.0
2	0.0	0.0	0.0	3.739843053746406E-5	0.0
3	0.0	0.0	0.0	3.739843053746406E-5	0.0
4	0.0	0.0	0.0	3.739843053746406E-5	0.0
5	0.0	0.0	0.0	3.739843053746406E-5	0.0
6	0.0	0.0	0.0	3.739843053746406E-5	0.0
7	0.0	0.0	0.0	3.739843053746406E-5	0.0
8	0.0	0.0	0.0	7.479686107492812E-5	0.0
9	0.0	0.0	0.0	1.121952916123922E-4	0.0
10-11	0.0	0.0	0.0	1.4959372214985627E-4	0.0
12-13	0.0	0.0	0.0	1.8699215268732034E-4	0.0
14-15	0.0	0.0	0.0	2.6178901376224846E-4	0.0
16-17	0.0	0.0	0.0	3.7398430537464067E-4	0.0
18-19	0.0	0.0	0.0	3.7398430537464067E-4	0.0
20-21	0.0	0.0	0.0	3.926835206433727E-4	0.0
22-23	0.0	0.0	0.0	5.42277242793229E-4	0.0
24-25	0.0	0.0	0.0	5.98374888599425E-4	0.0
26-27	0.0	0.0	0.0	7.292693954805493E-4	0.0
28-29	0.0	0.0	0.0	0.0012902458535425103	0.0
30-31	0.0	0.0	0.0	0.003721143838477674	0.0
32-33	0.0	0.0	0.0	0.008751232745766592	0.0
34-35	0.0	0.0	0.0	0.01664230158917151	0.0
36-37	0.0	0.0	0.0	0.030161834228464766	0.0
38-39	0.0	0.0	0.0	0.05637813403522708	0.0
40-41	0.0	0.0	0.0	0.08597899180562989	0.0
42-43	0.0	0.0	0.0	0.11578554094398874	0.0
44-45	0.0	0.0	0.0	0.15122055387823596	0.0
46-47	0.0	0.0	0.0	0.19658485012017984	0.0
48-49	0.0	0.0	0.0	0.24690443840833776	0.0
50-51	0.0	0.0	0.0	0.30251590461754685	0.0
52-53	0.0	0.0	0.0	0.3601655852910477	0.0
54-55	0.0	0.0	0.0	0.424060803864305	0.0
56-57	0.0	0.0	0.0	0.5008397817577188	0.0
58-59	0.0	0.0	0.0	0.5872488555145294	0.0
60-61	0.0	0.0	0.0	0.6824465604476443	0.0
62-63	0.0	0.0	0.0	0.7784296324220458	0.0
64-65	0.0	0.0	0.0	0.881555804629103	0.0
66-67	0.0	0.0	0.0	1.0012307823489879	0.0
68-69	0.0	0.0	0.0	1.135061066027303	0.0
70-71	0.0	0.0	0.0	1.28420600701071	0.0
72-73	0.0	0.0	0.0	1.4438225085446064	0.0
74-75	0.0	0.0	0.0	1.606019501785588	0.0
76-77	0.0	0.0	0.0	1.7810628559161885	0.0
78-79	0.0	0.0	0.0	1.97998510794496	0.0
80-81	0.0	0.0	0.0	2.2033098358994265	0.0
82-83	0.0	0.0	0.0	2.4424727991865094	0.0
84-85	0.0	0.0	0.0	2.6790178723359697	0.0
86-87	0.0	0.0	0.0	2.9204808391011063	0.0
88	0.0	0.0	0.0	3.1249754572799597	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCAACGC	2925	0.0	33.587803	4
GTATCAA	3185	0.0	32.471844	1
GGTATCA	2060	0.0	31.720592	1
CAACGCA	3115	0.0	31.538519	5
ATCAACG	3125	0.0	31.287762	3
AACGCAG	3390	0.0	28.975742	6
GTACATG	6890	0.0	27.633093	1
TATCAAC	3740	0.0	26.645597	2
TACATGG	6815	0.0	26.141743	2
ACATGGG	6955	0.0	24.939653	3
ACGCAGA	3960	0.0	24.211573	7
TACACCG	275	1.8189894E-12	23.930315	5
CGCAGAG	4080	0.0	23.176016	8
CATGGGG	4990	0.0	21.666487	4
TATACCG	195	1.1310702E-5	19.284502	5
CCGCGCA	125	0.006372694	18.820715	9
GGACGCG	255	2.9586408E-7	18.451683	9
GCAGAGT	5130	0.0	18.252058	9
GAGTACA	4765	0.0	18.15301	1
CCGGTCG	130	0.007990745	18.096842	9
>>END_MODULE