FastQCFastQC Report
Wed 25 May 2016
SRR1033083_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033083_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2673909
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48220.18033523205165172No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40300.1507156750659802No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA38400.054.4952241
GTATCAA69450.040.1750261
TCAACGC81550.033.4252474
ATCAACG81650.033.385563
CAACGCA83400.032.6274455
TATCAAC85950.031.9010262
AACGCAG86300.031.6399566
ACGCAGA99700.027.1517527
CGCAGAG102200.026.4875728
GTACATG69250.025.1819111
ATAACGC2800.025.1779293
TACATGG69350.023.9803662
GCAGAGT114350.023.46779
ACATGGG70800.022.1717513
GAGTACT77650.021.78891812-13
TATAACG2701.1114025E-920.9384752
AGAGTAC103950.020.8197610-11
GTGGTAT19550.020.732821
CAGAGTA112800.020.24872810-11
GAGTACA51000.019.4992981
Produced by FastQC (version 0.11.5)