##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1033083_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2673909 Sequences flagged as poor quality 0 Sequence length 100 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.70349177926399 34.0 31.0 34.0 31.0 34.0 2 32.69906829290002 34.0 31.0 34.0 31.0 34.0 3 32.801135715538564 34.0 31.0 34.0 31.0 34.0 4 36.29706358742949 37.0 37.0 37.0 35.0 37.0 5 36.282455012492946 37.0 37.0 37.0 35.0 37.0 6 36.35273825698631 37.0 37.0 37.0 35.0 37.0 7 36.305727307847796 37.0 37.0 37.0 35.0 37.0 8 36.30859688942294 37.0 37.0 37.0 35.0 37.0 9 38.104818451188876 39.0 39.0 39.0 37.0 39.0 10-11 38.07059870025495 39.0 39.0 39.0 35.0 39.0 12-13 38.044202887981605 39.0 38.5 39.0 35.0 39.0 14-15 39.539884865191745 41.0 39.0 41.0 37.0 41.0 16-17 39.54464381547764 41.0 39.0 41.0 37.0 41.0 18-19 39.561744995809505 41.0 39.5 41.0 37.0 41.0 20-21 39.55500991245401 41.0 40.0 41.0 37.0 41.0 22-23 39.48726452545692 41.0 39.0 41.0 36.5 41.0 24-25 39.43713604314881 41.0 39.0 41.0 36.0 41.0 26-27 39.33261659241208 41.0 39.0 41.0 36.0 41.0 28-29 39.27831949404411 41.0 39.0 41.0 36.0 41.0 30-31 39.27276507914069 41.0 39.0 41.0 36.0 41.0 32-33 39.23065930815147 41.0 39.0 41.0 35.5 41.0 34-35 39.150814219930446 41.0 39.0 41.0 35.0 41.0 36-37 39.06445956089007 41.0 39.0 41.0 35.0 41.0 38-39 38.971218541842674 41.0 39.0 41.0 35.0 41.0 40-41 38.870061583995565 41.0 39.0 41.0 35.0 41.0 42-43 38.75348731015154 41.0 39.0 41.0 35.0 41.0 44-45 38.59336779972692 41.0 38.5 41.0 35.0 41.0 46-47 38.3915752181544 41.0 38.0 41.0 34.5 41.0 48-49 38.186827412600806 40.0 38.0 41.0 34.0 41.0 50-51 38.05600153183971 40.0 38.0 41.0 34.0 41.0 52-53 37.929103795230134 40.0 38.0 41.0 34.0 41.0 54-55 37.788301883123175 40.0 38.0 41.0 33.0 41.0 56-57 37.648371728432046 40.0 37.0 41.0 33.0 41.0 58-59 37.47708149379803 40.0 37.0 41.0 33.0 41.0 60-61 37.29184052262063 40.0 36.5 41.0 33.0 41.0 62-63 37.06995077244588 39.5 36.0 41.0 32.5 41.0 64-65 36.8082042059023 39.0 35.5 41.0 32.0 41.0 66-67 36.53643205509238 39.0 35.0 41.0 32.0 41.0 68-69 36.231953854824525 38.5 35.0 40.5 31.5 41.0 70-71 35.90660246852081 37.0 35.0 40.0 31.0 41.0 72-73 35.55745614379546 37.0 35.0 39.5 31.0 41.0 74-75 35.21146512465458 36.5 35.0 39.0 31.0 41.0 76-77 34.85299368826688 36.0 35.0 39.0 31.0 40.5 78-79 34.48256859152649 35.5 35.0 37.5 30.0 39.5 80-81 34.140291610522276 35.0 34.0 37.0 30.0 39.0 82-83 33.83284640576774 35.0 34.0 37.0 30.0 39.0 84-85 33.597491163685824 35.0 34.0 36.0 30.0 38.0 86-87 33.37620745507794 35.0 34.0 36.0 30.0 37.0 88-89 33.15995832318901 35.0 34.0 36.0 30.0 37.0 90-91 32.96875211534873 35.0 34.0 35.0 30.0 36.5 92-93 32.789546502891454 35.0 34.0 35.0 29.0 36.0 94-95 32.632540972785534 35.0 34.0 35.0 29.0 36.0 96-97 32.486793305232155 35.0 34.0 35.0 29.0 36.0 98-99 32.359181632583606 35.0 34.0 35.0 29.0 36.0 100 32.25261592672002 35.0 34.0 35.0 29.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.12285985412194833 1101 2 0.07973489832738778 1101 3 0.1310242741079648 1101 4 0.10801272359377379 1101 5 0.13819986107023396 1101 6 0.14610537456687211 1101 7 0.16869619810603353 1101 8 0.18281533852560017 1101 9 0.22767505558660162 1101 10-11 0.2482712179705331 1101 12-13 0.24496062401991026 1101 14-15 0.30848980924147185 1101 16-17 0.3080080088550119 1101 18-19 0.36614197909153035 1101 20-21 0.3157638830455838 1101 22-23 0.33784248218804436 1101 24-25 0.3010494931571017 1101 26-27 0.3726810788511159 1101 28-29 0.3770671004468866 1101 30-31 0.3390923624091613 1101 32-33 0.3206453406593397 1101 34-35 0.3035053846491138 1101 36-37 0.3098122625975037 1101 38-39 0.18684692637695122 1101 40-41 0.31058828309234343 1101 42-43 0.2966522041384678 1101 44-45 0.2762923702241338 1101 46-47 0.19413081007765243 1101 48-49 0.15546489083140358 1101 50-51 0.2597262503203268 1101 52-53 0.2816686331264009 1101 54-55 0.2528777100029629 1101 56-57 0.26618244946817526 1101 58-59 0.26672058389274156 1101 60-61 0.2953956934186337 1101 62-63 0.20639482595622383 1101 64-65 0.2092220821635351 1101 66-67 0.2681768611364106 1101 68-69 0.26807274543759974 1101 70-71 0.3196038657732956 1101 72-73 0.3619586381330393 1101 74-75 0.3049259407805991 1101 76-77 0.3367288627922349 1101 78-79 0.3163769294728169 1101 80-81 0.3129129106684658 1101 82-83 0.2676145382943744 1101 84-85 0.33168723211088036 1101 86-87 0.336277337045388 1101 88-89 0.3002666919840422 1101 90-91 0.2571071799802809 1101 92-93 0.2790604661964977 1101 94-95 0.2812344172772825 1101 96-97 0.3376827612502993 1101 98-99 0.3773624194680636 1101 100 0.40119068160259275 1102 1 0.14651847054874878 1102 2 0.09339809765265983 1102 3 0.14087843010366896 1102 4 0.09246537532044385 1102 5 0.117348520283592 1102 6 0.11469788557160854 1102 7 0.1505146460054121 1102 8 0.1800944062331098 1102 9 0.18252087378802884 1102 10-11 0.18327285471998778 1102 12-13 0.19270741695376614 1102 14-15 0.26707092076765093 1102 16-17 0.2673895486318685 1102 18-19 0.22839830560956642 1102 20-21 0.2207573553036397 1102 22-23 0.2700139288049783 1102 24-25 0.28087083714022043 1102 26-27 0.2603491922759318 1102 28-29 0.22133393297082904 1102 30-31 0.21894347597647368 1102 32-33 0.2633159766039057 1102 34-35 0.212295807546667 1102 36-37 0.12192004928861166 1102 38-39 0.1894743906476748 1102 40-41 0.20814363410769943 1102 42-43 0.18111710260224356 1102 44-45 0.13398975258036216 1102 46-47 0.1262113689601989 1102 48-49 0.16917988846292786 1102 50-51 0.1588034702339911 1102 52-53 0.18286889038490983 1102 54-55 0.1433512505038479 1102 56-57 0.11321007394778348 1102 58-59 0.19698334957568875 1102 60-61 0.13565426132063152 1102 62-63 0.1454300295485993 1102 64-65 0.23695770668935268 1102 66-67 0.23820582197381412 1102 68-69 0.18652199272541026 1102 70-71 0.16451076361317263 1102 72-73 0.21074378492058088 1102 74-75 0.18377805950462545 1102 76-77 0.17780468858047271 1102 78-79 0.23862543810522396 1102 80-81 0.23190301699941074 1102 82-83 0.21331939963280888 1102 84-85 0.25954836643602164 1102 86-87 0.24319786034307356 1102 88-89 0.22880374606609877 1102 90-91 0.16463202968817114 1102 92-93 0.13642264226452738 1102 94-95 0.17042963719864446 1102 96-97 0.14045140960333669 1102 98-99 0.07307328863979023 1102 100 0.14682649136994286 1103 1 0.10883331060858126 1103 2 0.10109040534496927 1103 3 0.1255374381135752 1103 4 0.08432268555499434 1103 5 0.09128398418744155 1103 6 0.12231061004418109 1103 7 0.1300918233065076 1103 8 0.13433582340393002 1103 9 0.16156292762460112 1103 10-11 0.19660658105687645 1103 12-13 0.15059234551308975 1103 14-15 0.20160406938931885 1103 16-17 0.2469437352250452 1103 18-19 0.26254665462619897 1103 20-21 0.2545314421230387 1103 22-23 0.26811211091791165 1103 24-25 0.19130154447292824 1103 26-27 0.1801121374445671 1103 28-29 0.2374308181720366 1103 30-31 0.2432219582949955 1103 32-33 0.2090475895641788 1103 34-35 0.19225697388296226 1103 36-37 0.22321998223237927 1103 38-39 0.259061810603896 1103 40-41 0.187944307617542 1103 42-43 0.2161062335976851 1103 44-45 0.2642190701542546 1103 46-47 0.28086706100260983 1103 48-49 0.23026553621875934 1103 50-51 0.29063727394344596 1103 52-53 0.2807730515556699 1103 54-55 0.2423181424106744 1103 56-57 0.22755399135809284 1103 58-59 0.21760299336709465 1103 60-61 0.21227477463239808 1103 62-63 0.21842302180397155 1103 64-65 0.20330012086105853 1103 66-67 0.15437425838460683 1103 68-69 0.16240459273867458 1103 70-71 0.17958043577451832 1103 72-73 0.2023300700790429 1103 74-75 0.17514593293783065 1103 76-77 0.20950474826788223 1103 78-79 0.24965368277941735 1103 80-81 0.10433457911484822 1103 82-83 0.10009171056739774 1103 84-85 0.17007472100873855 1103 86-87 0.17163611334402162 1103 88-89 0.1742242475877589 1103 90-91 0.19648948729904703 1103 92-93 0.25863638216002727 1103 94-95 0.1904224157583414 1103 96-97 0.2830618128986728 1103 98-99 0.28075594318229946 1103 100 0.3022644569870465 1104 1 0.04130731179057534 1104 2 0.10263451853956695 1104 3 0.0847287120920015 1104 4 0.04403282786409335 1104 5 0.012894003258253406 1104 6 0.0678827901197252 1104 7 0.03936171091844187 1104 8 0.11297061533222319 1104 9 0.1112705278176378 1104 10-11 0.08444724895728939 1104 12-13 0.12279177981110934 1104 14-15 0.1617881236734462 1104 16-17 0.18355396813849723 1104 18-19 0.20885207497941138 1104 20-21 0.22092367270496283 1104 22-23 0.2175770032506179 1104 24-25 0.234611558843433 1104 26-27 0.1358711312916867 1104 28-29 0.17420859643599584 1104 30-31 0.13324360933232526 1104 32-33 0.17864255467819135 1104 34-35 0.15209196359099053 1104 36-37 0.17946414478722517 1104 38-39 0.15590627671647894 1104 40-41 0.11814187280390342 1104 42-43 0.20345539514401878 1104 44-45 0.21507665674746335 1104 46-47 0.08263236370144966 1104 48-49 0.013551624209846125 1104 50-51 0.09403720243673064 1104 52-53 0.07674897075787612 1104 54-55 0.007264472144157708 1104 56-57 0.059026905541983865 1104 58-59 0.06305747530402783 1104 60-61 0.12074342283218442 1104 62-63 0.023949096560819783 1104 64-65 0.011168337056389532 1104 66-67 -0.011936592018841452 1104 68-69 0.04909231008359427 1104 70-71 0.0847056932149357 1104 72-73 0.0885571082851726 1104 74-75 0.11187639057941823 1104 76-77 0.0962165317309811 1104 78-79 0.08563172488143067 1104 80-81 0.1748440708341974 1104 82-83 0.1163347876767915 1104 84-85 0.11988398467162398 1104 86-87 0.09083620356726385 1104 88-89 0.02698787779724654 1104 90-91 0.046293514287540916 1104 92-93 0.04012979136068395 1104 94-95 0.11905218979513421 1104 96-97 0.12199787028133358 1104 98-99 0.11710573976361616 1104 100 0.13190781687685416 1105 1 0.012459975964198122 1105 2 -0.011937618456606458 1105 3 0.042750005008983294 1105 4 0.014320723101981514 1105 5 0.05031259329165749 1105 6 0.03852238083461401 1105 7 0.06923298300500136 1105 8 0.0422307210672912 1105 9 0.07168395288476148 1105 10-11 0.09931931185624876 1105 12-13 0.11392794846202037 1105 14-15 0.11084453140760786 1105 16-17 0.1395167706811833 1105 18-19 0.10149960972474759 1105 20-21 0.12892438976179932 1105 22-23 0.15504206397972098 1105 24-25 0.16003265723597337 1105 26-27 0.15985471963996645 1105 28-29 0.14386421019585072 1105 30-31 0.1290014249939233 1105 32-33 0.12444711904237238 1105 34-35 0.15433514433292572 1105 36-37 0.18896772695638475 1105 38-39 0.135703682704424 1105 40-41 0.15776184205969201 1105 42-43 0.15026899057912857 1105 44-45 0.13195835449474203 1105 46-47 0.13227706083370094 1105 48-49 0.08169768059487836 1105 50-51 0.13589318454567945 1105 52-53 0.09724682149656871 1105 54-55 0.13047009669939058 1105 56-57 0.032248476974984897 1105 58-59 0.06004160285171878 1105 60-61 0.06945955681865001 1105 62-63 0.08293315082186581 1105 64-65 0.1652920134169662 1105 66-67 0.16512469684660402 1105 68-69 0.19979938211692883 1105 70-71 0.16682253422319349 1105 72-73 0.10116733383451759 1105 74-75 0.13161792856852372 1105 76-77 0.11057826774540302 1105 78-79 0.11741662230056704 1105 80-81 0.12104591710817658 1105 82-83 0.18508535834457973 1105 84-85 0.15902115977450393 1105 86-87 0.06626069498378229 1105 88-89 0.03780224575659474 1105 90-91 0.05295476060119597 1105 92-93 0.07152946500067969 1105 94-95 0.06867465977322951 1105 96-97 0.15083113828863048 1105 98-99 0.08544514157448901 1105 100 0.11129209791115358 1106 1 0.030934745339180836 1106 2 0.021756306607883857 1106 3 0.04190276050645281 1106 4 0.0704739625214259 1106 5 0.048231093717788553 1106 6 0.060492883186270774 1106 7 0.09361217207369776 1106 8 0.09708207065074959 1106 9 0.08828247673221057 1106 10-11 0.1659209157845254 1106 12-13 0.12319095815189485 1106 14-15 0.1737539440076148 1106 16-17 0.21111032829520582 1106 18-19 0.13071582758870903 1106 20-21 0.14298321231992617 1106 22-23 0.19501665490052744 1106 24-25 0.16567149140315252 1106 26-27 0.17937938378413776 1106 28-29 0.17613288342404587 1106 30-31 0.1784142726778981 1106 32-33 0.19226370372173562 1106 34-35 0.18668993281075785 1106 36-37 0.1161008575759439 1106 38-39 0.09526393607124106 1106 40-41 0.1331787325879077 1106 42-43 0.11372665756714184 1106 44-45 0.08220552304311468 1106 46-47 0.14741289557370152 1106 48-49 0.11616584907084615 1106 50-51 0.16788852441513313 1106 52-53 0.10575752938170524 1106 54-55 0.21724207037708254 1106 56-57 0.18498851053198706 1106 58-59 0.20311672380045565 1106 60-61 0.1758495179143722 1106 62-63 0.1585026799949958 1106 64-65 0.15766887686586983 1106 66-67 0.15118237755208952 1106 68-69 0.20420072236488096 1106 70-71 0.13304582801718112 1106 72-73 0.14086373312476752 1106 74-75 0.19674677755819658 1106 76-77 0.18885423536708856 1106 78-79 0.2080873749961185 1106 80-81 0.19164914935134902 1106 82-83 0.21023993394754115 1106 84-85 0.22929040964464065 1106 86-87 0.14181641142327805 1106 88-89 0.15393971008163732 1106 90-91 0.10171510745004753 1106 92-93 0.13404684999300542 1106 94-95 0.14570497284915973 1106 96-97 0.09370875302322901 1106 98-99 0.14048520167734324 1106 100 0.14104580575691017 1107 1 -0.008054583202387278 1107 2 -0.002179297924733703 1107 3 0.014415503914712247 1107 4 -7.681072281044976E-4 1107 5 0.012342966784885334 1107 6 0.03510446899188935 1107 7 0.035225359209270835 1107 8 0.0341962540335885 1107 9 0.03258919865087506 1107 10-11 0.01808790638435198 1107 12-13 0.03226078102768781 1107 14-15 0.08783831331587066 1107 16-17 0.023736692787238667 1107 18-19 0.035396050552513714 1107 20-21 -0.006557780504643063 1107 22-23 0.05038752780871647 1107 24-25 0.09337327731389422 1107 26-27 0.10015633441184235 1107 28-29 0.11892842659272418 1107 30-31 0.08560161605926453 1107 32-33 0.04244828450333671 1107 34-35 0.045025099728007945 1107 36-37 0.02306878208118235 1107 38-39 0.005571122497826764 1107 40-41 0.03727190309898276 1107 42-43 0.033204208387971335 1107 44-45 0.046791071957024144 1107 46-47 0.1205680045497104 1107 48-49 0.1415924629302978 1107 50-51 0.11537376406455735 1107 52-53 0.11275479122971177 1107 54-55 0.07478910180471132 1107 56-57 0.14823279280611956 1107 58-59 0.11657003464183191 1107 60-61 0.086252356302289 1107 62-63 0.08795401056573127 1107 64-65 0.015912975781610328 1107 66-67 0.0568356223844404 1107 68-69 0.08805846070023193 1107 70-71 0.0856706034031447 1107 72-73 0.14573250963533013 1107 74-75 0.12984113378687567 1107 76-77 0.10105062442914203 1107 78-79 0.09958535194184748 1107 80-81 0.0858278259927161 1107 82-83 0.10406289530781265 1107 84-85 0.09898157299251409 1107 86-87 0.09501907134236376 1107 88-89 0.08765198409242458 1107 90-91 0.047755788565353896 1107 92-93 0.054713174390677466 1107 94-95 0.033249131661975184 1107 96-97 0.03714421698108339 1107 98-99 0.13645284503304822 1107 100 0.13684567618364696 1108 1 -0.06012577559964427 1108 2 0.04741392119617416 1108 3 0.01877236835309759 1108 4 -0.027642742374140994 1108 5 -0.025500702825745236 1108 6 -0.017286822934288182 1108 7 -0.04432013951628022 1108 8 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-0.11402761288189112 2214 60-61 -0.166741192605663 2214 62-63 -0.16035875214392092 2214 64-65 -0.2072882764079651 2214 66-67 -0.139490877444004 2214 68-69 -0.1391006049802357 2214 70-71 -0.12411889957702016 2214 72-73 -0.13194021083894825 2214 74-75 -0.1530648000918191 2214 76-77 -0.1826701143499534 2214 78-79 -0.07769109323341894 2214 80-81 -0.10362464380995107 2214 82-83 -0.10981268880123451 2214 84-85 -0.059483135083091554 2214 86-87 -0.0982004304374513 2214 88-89 -0.0626637189470145 2214 90-91 -0.016513786988269885 2214 92-93 -0.08652022384661961 2214 94-95 -0.10950616342794461 2214 96-97 0.002866111496750534 2214 98-99 -0.03744599190081033 2214 100 -0.007390353266387706 2215 1 -0.20749902879100546 2215 2 -0.11334959723417626 2215 3 -0.14665799814788727 2215 4 -0.1080856701617634 2215 5 -0.14742119805052312 2215 6 -0.17333274975547397 2215 7 -0.20402829282728163 2215 8 -0.15271191833141273 2215 9 -0.2378913481917877 2215 10-11 -0.21949661099898776 2215 12-13 -0.23182805824976072 2215 14-15 -0.3688144378884317 2215 16-17 -0.3803030991192742 2215 18-19 -0.3573835956638405 2215 20-21 -0.3646494124856332 2215 22-23 -0.3422913565927175 2215 24-25 -0.34387924610795295 2215 26-27 -0.3443349239729656 2215 28-29 -0.36416572829522664 2215 30-31 -0.3178519861370006 2215 32-33 -0.3619360644116085 2215 34-35 -0.37059864525448916 2215 36-37 -0.35362542717798817 2215 38-39 -0.3259322623165346 2215 40-41 -0.35543936718981683 2215 42-43 -0.37824412556664555 2215 44-45 -0.3689591273011388 2215 46-47 -0.40707389094633584 2215 48-49 -0.375629157455613 2215 50-51 -0.3035061785161446 2215 52-53 -0.23988329416194176 2215 54-55 -0.3067070232161413 2215 56-57 -0.30623537840555315 2215 58-59 -0.28474270078658037 2215 60-61 -0.23464359489542375 2215 62-63 -0.2582998330058004 2215 64-65 -0.27394797600305765 2215 66-67 -0.29030736568478943 2215 68-69 -0.22232757495822142 2215 70-71 -0.24590852010808106 2215 72-73 -0.26204669378511625 2215 74-75 -0.24592752903124193 2215 76-77 -0.2523705918797461 2215 78-79 -0.31370068804158535 2215 80-81 -0.2942266893929002 2215 82-83 -0.2631954874367324 2215 84-85 -0.2868555996216884 2215 86-87 -0.31911322372036466 2215 88-89 -0.22484011251535918 2215 90-91 -0.2471744556314448 2215 92-93 -0.25524829206702293 2215 94-95 -0.2693237185638466 2215 96-97 -0.2654642628696493 2215 98-99 -0.2659967431667525 2215 100 -0.25800316564759385 2216 1 -0.21548843716486488 2216 2 -0.22507530876227833 2216 3 -0.22217787165551783 2216 4 -0.20063213912624178 2216 5 -0.2197778263590493 2216 6 -0.2666518438484715 2216 7 -0.24477476049852243 2216 8 -0.20286278485249198 2216 9 -0.24796806107812586 2216 10-11 -0.29383708471237924 2216 12-13 -0.31217954014832117 2216 14-15 -0.4467576878452988 2216 16-17 -0.44544383753823524 2216 18-19 -0.37008422210193714 2216 20-21 -0.37553261293867735 2216 22-23 -0.3887849521676614 2216 24-25 -0.42606168195055005 2216 26-27 -0.3818616097863696 2216 28-29 -0.39851534316530035 2216 30-31 -0.3936248920344738 2216 32-33 -0.4096866541115105 2216 34-35 -0.5147097375342327 2216 36-37 -0.4415131733655713 2216 38-39 -0.4128565455644235 2216 40-41 -0.5081844878507056 2216 42-43 -0.44746737228361155 2216 44-45 -0.48218325171887955 2216 46-47 -0.39106614648760996 2216 48-49 -0.4546152939351913 2216 50-51 -0.4349615673913618 2216 52-53 -0.35031700052661563 2216 54-55 -0.36176696483661885 2216 56-57 -0.332119766596378 2216 58-59 -0.3176059417996768 2216 60-61 -0.37100133743130925 2216 62-63 -0.39211678661833815 2216 64-65 -0.4147978091922653 2216 66-67 -0.4145103221649151 2216 68-69 -0.3869088939869769 2216 70-71 -0.48034171053852504 2216 72-73 -0.43761688449360037 2216 74-75 -0.5462625970062547 2216 76-77 -0.47402351958531597 2216 78-79 -0.548130772997169 2216 80-81 -0.5549187117087584 2216 82-83 -0.4779262691417614 2216 84-85 -0.5355305828351149 2216 86-87 -0.5110680699457504 2216 88-89 -0.6311300689161854 2216 90-91 -0.6382591988503634 2216 92-93 -0.5159067922707052 2216 94-95 -0.504777844489233 2216 96-97 -0.6200638880345792 2216 98-99 -0.6855561809345687 2216 100 -0.7597322770325512 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 17.0 3 4.0 4 4.0 5 16.0 6 53.0 7 110.0 8 334.0 9 588.0 10 1104.0 11 1772.0 12 2903.0 13 4445.0 14 5480.0 15 7242.0 16 8684.0 17 12551.0 18 14673.0 19 7778.0 20 5217.0 21 4886.0 22 4849.0 23 5361.0 24 6081.0 25 7476.0 26 9493.0 27 12406.0 28 15821.0 29 21047.0 30 26747.0 31 34414.0 32 44564.0 33 58282.0 34 80921.0 35 121666.0 36 216680.0 37 497912.0 38 953027.0 39 477421.0 40 1880.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.479960729615016 20.2124626602094 13.804872281453722 23.502704328721858 2 15.486492920757094 20.494056735169018 40.404790603249346 23.61465974082454 3 17.94199276770217 25.65360143224779 30.716770905054492 25.687634894995547 4 12.016530471043627 17.195766404248555 39.49511004731006 31.29259307739776 5 12.219319684132383 37.95894020169004 36.29489454561774 13.526845568559839 6 30.166842065502674 38.96139206090267 17.743106900541754 13.128658973052904 7 26.385901689249707 33.066121547143155 23.004223404760594 17.54375335884654 8 24.70980568443523 36.51567860853331 20.595550017240747 18.17896568979071 9 24.76527810033924 16.480254189652676 21.67758887830513 37.07687883170295 10-11 23.517004220434984 26.868052036448702 29.362222525566633 20.25272121754968 12-13 24.525909445684203 24.24091470577346 29.578699200309362 21.654476648232983 14-15 21.54989370393315 26.139669796864094 27.128618708369558 25.1818177908332 16-17 20.657816815141747 29.7207399438761 27.610474640932527 22.010968600049626 18-19 20.410909773603763 28.20887573787462 30.47044462462265 20.90976986389897 20-21 21.670595371794626 27.486425304675667 30.110804069996398 20.73217525353331 22-23 21.195094144092174 27.301160306091482 30.216688579050917 21.287056970765434 24-25 21.05990369781684 27.44230751250526 30.208255808517603 21.2895329811603 26-27 20.931120989294314 27.349540335374538 30.27353990753602 21.44579876779513 28-29 20.89242221808761 27.45339443005121 30.357838402858743 21.296344949002442 30-31 21.210626405949352 27.90961159801863 29.873163033092048 21.00659896293997 32-33 20.864565697635935 27.57477909682042 30.3205344684505 21.240120737093147 34-35 20.96767317062772 27.31816976568761 30.390862217076197 21.32329484660847 36-37 21.347884314686848 27.7534874971437 29.71108964441198 21.187538543757473 38-39 21.2635321546096 27.90250154362022 29.768122250981616 21.065844050788566 40-41 21.04228007929969 27.677813775871783 29.964093739984225 21.315812404844298 42-43 21.087217253840727 27.57081486318345 29.68999692958885 21.65197095338697 44-45 21.29388098099075 27.797580246747366 29.57423008786013 21.33430868440175 46-47 21.38477786641206 28.06763431365839 29.174085580324537 21.373502239605013 48-49 21.411686037183763 27.989639886772512 28.95652021067284 21.642153865370886 50-51 21.405926678880995 28.02139115429882 28.86726885619518 21.70541331062501 52-53 21.49904877091928 27.97103416758012 28.78734841013662 21.742568651363978 54-55 21.540299239802103 28.42564948919354 28.589679005530854 21.444372265473508 56-57 21.344275366139982 28.11458804319818 28.747799569843252 21.793337020818583 58-59 21.172560472327216 27.683515033608096 29.189643327428122 21.954281166636562 60-61 21.45286170920551 28.086034341482822 28.81599560792832 21.645108341383345 62-63 21.34034853093355 28.069990414782254 28.764198781633933 21.825462272650267 64-65 21.29214195397076 27.813250189142565 28.957025089485093 21.937582767401583 66-67 21.222618271601615 27.923388567075396 28.770724807762715 22.083268353560275 68-69 21.58319564441778 28.06523948895659 28.686185955955008 21.665378910670626 70-71 21.64617824431913 27.82000580797735 28.72731060169037 21.80650534601315 72-73 21.406122932489772 27.993938438575732 28.83622919867751 21.763709430256984 74-75 21.484788063593168 28.009817856179176 28.622106220362852 21.8832878598648 76-77 21.517299205021562 28.04910339132708 28.48666502861541 21.94693237503595 78-79 21.605596899520517 28.184130424782595 28.537919577667004 21.672353098029888 80-81 21.558699267626537 27.94279835252434 28.626684752547675 21.87181762730145 82-83 21.323712691950178 28.050750522597394 28.7994873412597 21.826049444192734 84-85 21.366022553497523 27.978177267812782 28.617653031572875 22.038147147116824 86-87 21.487919030493153 28.09115015900958 28.485612096658226 21.93531871383904 88-89 21.188682579471305 27.95080488099056 28.66614245229478 22.19437008724336 90-91 21.178205696626826 27.882968555961817 28.65051477357237 22.28831097383899 92-93 21.363418856096654 28.04671922557728 28.50158112426902 22.088280794057045 94-95 21.09622664054493 27.80945966186723 28.653753771891555 22.440559925696284 96-97 21.116447258808897 27.857738963084465 28.621136567670874 22.40467721043576 98-99 21.077454336906946 27.938320160648967 28.550215611664516 22.434009890779567 100 21.17398161268764 27.785313561531076 28.463870685202824 22.576834140578455 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 20.0 1 22.5 2 98.5 3 188.0 4 133.0 5 94.0 6 237.5 7 540.0 8 956.5 9 1174.0 10 985.5 11 784.0 12 878.0 13 1105.0 14 1333.0 15 1544.0 16 1720.0 17 1959.0 18 2365.5 19 2869.0 20 3396.0 21 4234.0 22 5618.5 23 7614.0 24 10261.0 25 13612.5 26 18084.5 27 24135.5 28 31876.0 29 39618.0 30 46518.0 31 54228.0 32 62374.0 33 70090.0 34 77752.0 35 84182.5 36 88966.5 37 93369.5 38 96651.5 39 97901.5 40 98890.0 41 99592.0 42 99368.5 43 98454.5 44 95949.0 45 93541.0 46 91674.0 47 88637.5 48 85942.0 49 83839.5 50 81203.5 51 78004.0 52 72171.5 53 67591.5 54 64948.5 55 60874.0 56 55466.0 57 50318.0 58 45346.5 59 39674.5 60 34750.5 61 29314.5 62 24342.5 63 20020.5 64 15768.0 65 12429.5 66 9495.0 67 7001.5 68 5401.5 69 4226.0 70 3118.5 71 2190.0 72 1594.0 73 1189.5 74 849.5 75 511.0 76 301.5 77 191.0 78 109.0 79 64.0 80 32.5 81 14.0 82 10.5 83 7.5 84 3.5 85 1.5 86 2.0 87 1.5 88 0.0 89 0.0 90 0.5 91 0.5 92 0.0 93 0.0 94 0.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04326998413184592 2 0.24697923526941268 3 0.0026178901376224845 4 0.00220650740171038 5 1.4959372214985625E-4 6 1.1219529161239218E-4 7 0.0 8 4.113827359121047E-4 9 0.0 10-11 5.235780275244969E-4 12-13 0.0 14-15 3.552850901059086E-4 16-17 1.3089450688112423E-4 18-19 4.861795969870329E-4 20-21 0.0 22-23 9.349607634366017E-5 24-25 0.0012715466382737781 26-27 1.1219529161239218E-4 28-29 2.4308979849351644E-4 30-31 1.4959372214985625E-4 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 7.479686107492812E-5 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 2.6178901376224846E-4 70-71 1.869921526873203E-5 72-73 3.926835206433727E-4 74-75 2.0569136795605235E-4 76-77 0.0 78-79 0.0 80-81 0.0 82-83 2.0569136795605235E-4 84-85 0.0 86-87 5.048788122557649E-4 88-89 0.0024869956307413603 90-91 0.0023748003391289682 92-93 7.666678260180133E-4 94-95 6.918709649430852E-4 96-97 2.804882290309805E-4 98-99 1.682929374185883E-4 100 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2673909.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.67179151618733 #Duplication Level Percentage of deduplicated Percentage of total 1 78.3253658534318 52.22092462609103 2 13.656781325201134 18.210441545919412 3 3.9326526923884586 7.865910012374884 4 1.6200554359489854 4.3204799308102695 5 0.7605591026221502 2.535391896288126 6 0.43571911209026254 1.7430104280540148 7 0.28333135696735234 1.3223146413208038 8 0.17833470455374809 0.9511915393686685 9 0.14582870187391378 0.8750394727572435 >10 0.624511945019971 7.193482794971537 >50 0.027782522240027724 1.251434415982006 >100 0.008572300610821445 0.9603555699456982 >500 2.805261397357451E-4 0.09980017009685274 >1k 2.2442091166816587E-4 0.4502229560194946 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4822 0.18033523205165172 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4030 0.1507156750659802 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 3.739843053746406E-5 0.0 0.0 2 0.0 0.0 3.739843053746406E-5 0.0 0.0 3 0.0 0.0 3.739843053746406E-5 3.739843053746406E-5 0.0 4 0.0 0.0 3.739843053746406E-5 7.479686107492812E-5 0.0 5 0.0 0.0 3.739843053746406E-5 7.479686107492812E-5 0.0 6 0.0 0.0 3.739843053746406E-5 1.121952916123922E-4 0.0 7 0.0 0.0 3.739843053746406E-5 1.121952916123922E-4 0.0 8 0.0 0.0 3.739843053746406E-5 1.121952916123922E-4 0.0 9 0.0 0.0 3.739843053746406E-5 1.4959372214985625E-4 0.0 10-11 0.0 0.0 3.739843053746406E-5 1.4959372214985625E-4 0.0 12-13 0.0 0.0 3.739843053746406E-5 1.4959372214985625E-4 0.0 14-15 0.0 0.0 3.739843053746406E-5 3.1788665956844454E-4 0.0 16-17 0.0 0.0 3.739843053746406E-5 4.3008195118083675E-4 0.0 18-19 0.0 0.0 3.739843053746406E-5 4.487811664495688E-4 0.0 20-21 0.0 0.0 3.739843053746406E-5 4.674803817183008E-4 0.0 22-23 0.0 0.0 3.739843053746406E-5 5.79675673330693E-4 0.0 24-25 0.0 0.0 3.739843053746406E-5 6.17074103868157E-4 0.0 26-27 0.0 0.0 3.739843053746406E-5 7.479686107492813E-4 0.0 28-29 0.0 0.0 3.739843053746406E-5 0.0013463434993487063 0.0 30-31 0.0 0.0 3.739843053746406E-5 0.003926835206433727 0.0 32-33 0.0 0.0 3.739843053746406E-5 0.009087818620603767 0.0 34-35 0.0 0.0 3.739843053746406E-5 0.017034985109814882 0.0 36-37 0.0 0.0 3.739843053746406E-5 0.030648013825451802 0.0 38-39 0.0 0.0 3.739843053746406E-5 0.05684561441694538 0.0 40-41 0.0 0.0 3.739843053746406E-5 0.08631557768046706 0.0 42-43 0.0 0.0 3.739843053746406E-5 0.11615952524936338 0.0 44-45 0.0 0.0 3.739843053746406E-5 0.15185632719737285 0.0 46-47 0.0 0.0 3.739843053746406E-5 0.19729542030039168 0.0 48-49 0.0 0.0 3.739843053746406E-5 0.24806378975499913 0.0 50-51 0.0 0.0 3.739843053746406E-5 0.30388094733216425 0.0 52-53 0.0 0.0 3.739843053746406E-5 0.3617176201583524 0.0 54-55 0.0 0.0 3.739843053746406E-5 0.42578113166902837 0.0 56-57 0.0 0.0 3.739843053746406E-5 0.5031210860205041 0.0 58-59 0.0 0.0 3.739843053746406E-5 0.5897545503605396 0.0 60-61 0.0 0.0 3.739843053746406E-5 0.6855880286127912 0.0 62-63 0.0 0.0 3.739843053746406E-5 0.7824125652742857 0.0 64-65 0.0 0.0 3.739843053746406E-5 0.8863054053073609 0.0 66-67 0.0 0.0 3.739843053746406E-5 1.0069714414364888 0.0 68-69 0.0 0.0 3.739843053746406E-5 1.1417553850935092 0.0 70-71 0.0 0.0 3.739843053746406E-5 1.2921905719304583 0.0 72-73 0.0 0.0 3.739843053746406E-5 1.452854229519404 0.0 74-75 0.0 0.0 3.739843053746406E-5 1.6159674843085536 0.0 76-77 0.0 0.0 3.739843053746406E-5 1.7918149046957095 0.0 78-79 0.0 0.0 3.739843053746406E-5 1.9919152072864108 0.0 80-81 0.0 0.0 3.739843053746406E-5 2.2166236771707637 0.0 82-83 0.0 0.0 3.739843053746406E-5 2.456796398082358 0.0 84-85 0.0 0.0 3.739843053746406E-5 2.6952674904044978 0.0 86-87 0.0 0.0 3.739843053746406E-5 2.938245093606402 0.0 88 0.0 0.0 3.739843053746406E-5 3.1435624772570794 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3840 0.0 54.495224 1 GTATCAA 6945 0.0 40.175026 1 TCAACGC 8155 0.0 33.425247 4 ATCAACG 8165 0.0 33.38556 3 CAACGCA 8340 0.0 32.627445 5 TATCAAC 8595 0.0 31.901026 2 AACGCAG 8630 0.0 31.639956 6 ACGCAGA 9970 0.0 27.151752 7 CGCAGAG 10220 0.0 26.487572 8 GTACATG 6925 0.0 25.181911 1 ATAACGC 280 0.0 25.177929 3 TACATGG 6935 0.0 23.980366 2 GCAGAGT 11435 0.0 23.4677 9 ACATGGG 7080 0.0 22.171751 3 GAGTACT 7765 0.0 21.788918 12-13 TATAACG 270 1.1114025E-9 20.938475 2 AGAGTAC 10395 0.0 20.81976 10-11 GTGGTAT 1955 0.0 20.73282 1 CAGAGTA 11280 0.0 20.248728 10-11 GAGTACA 5100 0.0 19.499298 1 >>END_MODULE