FastQCFastQC Report
Wed 25 May 2016
SRR1033082_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033082_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2437440
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA25750.034.8725661
TCAACGC25750.032.3118134
ATCAACG26500.031.5747183
CAACGCA26700.031.3382035
GGTATCA17350.030.620111
AACGCAG27900.030.3223116
CGGACCG856.812103E-427.6513545
GTACATG61350.026.8980221
TATCAAC32700.026.306842
TACATGG63450.025.0409282
ACGCAGA33600.025.038987
ACATGGG63750.024.111983
CGCAGAG35100.023.315228
CATGGGG45050.021.599364
CTATGCG1150.00393894320.4535059
GTACCGT1200.00506598919.583166
CTGTCGC5350.018.46559
GTCTCGC5800.017.8329221
GCAGAGT45950.017.5067759
TACCGTA2705.45042E-717.4076087
Produced by FastQC (version 0.11.5)