FastQCFastQC Report
Wed 25 May 2016
SRR1033082_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033082_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2437440
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32760.13440330838912956No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28630.11745930156229487No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA30900.055.5293461
GTATCAA59300.041.733451
TCAACGC67050.036.1675454
ATCAACG68400.035.386453
CAACGCA68650.035.32465
AACGCAG71550.033.8928576
TATCAAC73250.033.1913762
ACGCAGA81550.029.3333667
CGCAGAG84000.028.477818
GCAGAGT95350.024.6936429
GTACATG61850.024.5412031
TACATGG64450.022.9556642
GTGGTAT15900.022.2353441
ACATGGG64850.021.9593033
GAGTACT63400.021.86758612-13
AGAGTAC86150.020.97534810-11
GAGTACA46000.020.7001361
CAGAGTA94100.020.52675610-11
CGTACAC2151.1954344E-619.673883
TATACTG17200.019.673075
Produced by FastQC (version 0.11.5)