##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1033082_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2437440 Sequences flagged as poor quality 0 Sequence length 100 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.67229634370487 34.0 31.0 34.0 31.0 34.0 2 32.670209317972954 34.0 31.0 34.0 31.0 34.0 3 32.77686384075095 34.0 31.0 34.0 31.0 34.0 4 36.2782419259551 37.0 37.0 37.0 35.0 37.0 5 36.25926217670999 37.0 37.0 37.0 35.0 37.0 6 36.329362774058026 37.0 37.0 37.0 35.0 37.0 7 36.27716579690167 37.0 37.0 37.0 35.0 37.0 8 36.281748473808584 37.0 37.0 37.0 35.0 37.0 9 38.06816742155704 39.0 39.0 39.0 35.0 39.0 10-11 38.03202683963502 39.0 38.5 39.0 35.0 39.0 12-13 38.00143962518052 39.0 38.0 39.0 35.0 39.0 14-15 39.48832032788499 41.0 39.0 41.0 36.0 41.0 16-17 39.49245560916371 41.0 39.0 41.0 36.0 41.0 18-19 39.50493099317316 41.0 39.5 41.0 36.5 41.0 20-21 39.49893474300906 41.0 39.0 41.0 36.5 41.0 22-23 39.42753052382828 41.0 39.0 41.0 36.0 41.0 24-25 39.377754119075746 41.0 39.0 41.0 36.0 41.0 26-27 39.2706140458842 41.0 39.0 41.0 36.0 41.0 28-29 39.22071456117894 41.0 39.0 41.0 36.0 41.0 30-31 39.22362519692793 41.0 39.0 41.0 36.0 41.0 32-33 39.18709034889064 41.0 39.0 41.0 35.0 41.0 34-35 39.11451645989234 41.0 39.0 41.0 35.0 41.0 36-37 39.02812807699882 41.0 39.0 41.0 35.0 41.0 38-39 38.94151856045687 41.0 39.0 41.0 35.0 41.0 40-41 38.83943932978863 41.0 39.0 41.0 35.0 41.0 42-43 38.72197961795983 41.0 38.5 41.0 35.0 41.0 44-45 38.563195606866216 41.0 38.0 41.0 35.0 41.0 46-47 38.376507524287774 40.0 38.0 41.0 34.5 41.0 48-49 38.1851036333202 40.0 38.0 41.0 34.0 41.0 50-51 38.05262796212419 40.0 38.0 41.0 34.0 41.0 52-53 37.917670178547986 40.0 38.0 41.0 33.5 41.0 54-55 37.76954181436261 40.0 37.5 41.0 33.0 41.0 56-57 37.623228264080346 40.0 37.0 41.0 33.0 41.0 58-59 37.44347512143889 40.0 37.0 41.0 33.0 41.0 60-61 37.25431559340948 40.0 36.0 41.0 33.0 41.0 62-63 37.0233517542996 39.0 36.0 41.0 32.5 41.0 64-65 36.75636590028883 39.0 35.0 41.0 32.0 41.0 66-67 36.482437926677164 39.0 35.0 41.0 32.0 41.0 68-69 36.17648988282788 38.0 35.0 40.5 31.0 41.0 70-71 35.85329874786662 37.0 35.0 40.0 31.0 41.0 72-73 35.51096129545753 37.0 35.0 39.5 31.0 41.0 74-75 35.16318432453722 36.0 35.0 39.0 31.0 41.0 76-77 34.80813476434292 36.0 35.0 39.0 30.5 40.5 78-79 34.44102849711172 35.0 34.0 37.5 30.0 39.5 80-81 34.10456339438099 35.0 34.0 37.0 30.0 39.0 82-83 33.801986100170666 35.0 34.0 37.0 30.0 39.0 84-85 33.57561252789812 35.0 34.0 36.0 30.0 37.5 86-87 33.35849436293816 35.0 34.0 36.0 30.0 37.0 88-89 33.14544214421689 35.0 34.0 36.0 30.0 37.0 90-91 32.95633636110017 35.0 34.0 35.0 29.0 36.0 92-93 32.77898286727058 35.0 34.0 35.0 29.0 36.0 94-95 32.61638460844164 35.0 34.0 35.0 29.0 36.0 96-97 32.465549305829065 35.0 34.0 35.0 29.0 36.0 98-99 32.33578508599186 35.0 34.0 35.0 29.0 35.5 100 32.22903168898517 35.0 34.0 35.0 29.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.10690744662148433 1101 2 0.11744214431315925 1101 3 0.16647883539437203 1101 4 0.08145311210176231 1101 5 0.06148196346006074 1101 6 0.07288318460584264 1101 7 0.12887574334452978 1101 8 0.10500997875855234 1101 9 0.16863366540733438 1101 10-11 0.18048370175898043 1101 12-13 0.24541481872653748 1101 14-15 0.25699394105780016 1101 16-17 0.29730927099947024 1101 18-19 0.2930072226960121 1101 20-21 0.28354890385570997 1101 22-23 0.30963382796337413 1101 24-25 0.3152278412407554 1101 26-27 0.31261284062169636 1101 28-29 0.3199454409898408 1101 30-31 0.3391700538262086 1101 32-33 0.23861103803086792 1101 34-35 0.2663480780435563 1101 36-37 0.2804613430948706 1101 38-39 0.29840090471719094 1101 40-41 0.32238307626875695 1101 42-43 0.28152257649725243 1101 44-45 0.33418187287718837 1101 46-47 0.36754110819317987 1101 48-49 0.3430658459833893 1101 50-51 0.2621638386514391 1101 52-53 0.3277550153167397 1101 54-55 0.4266000107156174 1101 56-57 0.3977061629156253 1101 58-59 0.3597984891858417 1101 60-61 0.3542139885554292 1101 62-63 0.3000793576179248 1101 64-65 0.3557584696063856 1101 66-67 0.33276453328976885 1101 68-69 0.30949165089747055 1101 70-71 0.3126769447364026 1101 72-73 0.37666875382230813 1101 74-75 0.3472599174881239 1101 76-77 0.3133679280923616 1101 78-79 0.32297663737364957 1101 80-81 0.2684583174267985 1101 82-83 0.3126667598378532 1101 84-85 0.2563261728154842 1101 86-87 0.34835095042195974 1101 88-89 0.32953590336279603 1101 90-91 0.3472587121635442 1101 92-93 0.31880237117386656 1101 94-95 0.2916965356312389 1101 96-97 0.3774279729684267 1101 98-99 0.38214910242445654 1101 100 0.3450385253128232 1102 1 0.06731421008721128 1102 2 0.049708971961820225 1102 3 0.0999141404484547 1102 4 0.08372395812754974 1102 5 0.09362027711198806 1102 6 0.07377009784407562 1102 7 0.101958912640427 1102 8 0.08106980257956309 1102 9 0.14229801795789143 1102 10-11 0.11631109857280109 1102 12-13 0.1529133974802548 1102 14-15 0.21238222369230186 1102 16-17 0.20389385413450611 1102 18-19 0.23320664160534932 1102 20-21 0.17390276177551556 1102 22-23 0.20038757922027628 1102 24-25 0.2563558385889948 1102 26-27 0.23865956305182578 1102 28-29 0.18197594362030145 1102 30-31 0.2239258216788258 1102 32-33 0.21404398576048322 1102 34-35 0.2520680712289831 1102 36-37 0.229446101038576 1102 38-39 0.15045152701010522 1102 40-41 0.20587652742510443 1102 42-43 0.18621079117118455 1102 44-45 0.22695323832914482 1102 46-47 0.3498227501354165 1102 48-49 0.3818157788561223 1102 50-51 0.37009379111607643 1102 52-53 0.324591284040622 1102 54-55 0.321352463870042 1102 56-57 0.29215265461314743 1102 58-59 0.42838598332956224 1102 60-61 0.427630479456667 1102 62-63 0.33801774497096204 1102 64-65 0.4036301185668876 1102 66-67 0.43399754546155833 1102 68-69 0.38345631695550964 1102 70-71 0.3614444418059506 1102 72-73 0.38402932817247404 1102 74-75 0.30510163231667775 1102 76-77 0.2897265376968363 1102 78-79 0.2519477476493961 1102 80-81 0.164629609547994 1102 82-83 0.2361184809809913 1102 84-85 0.33042421381033904 1102 86-87 0.33331962744998833 1102 88-89 0.3022070501187457 1102 90-91 0.1760591340005604 1102 92-93 0.24692277064129087 1102 94-95 0.2768472895861862 1102 96-97 0.26370840939750906 1102 98-99 0.25475066829529425 1102 100 0.2430299848725852 1103 1 0.13932692514909206 1103 2 0.06419921978486087 1103 3 0.12849375320893586 1103 4 0.08277741178923037 1103 5 0.12614909796636908 1103 6 0.16045806306399868 1103 7 0.1406005523502074 1103 8 0.15186038100418386 1103 9 0.15772564653941146 1103 10-11 0.1480115912716542 1103 12-13 0.18667031704810455 1103 14-15 0.24634781592757804 1103 16-17 0.24481585451607657 1103 18-19 0.24388080470781404 1103 20-21 0.288858220616909 1103 22-23 0.2911489772862126 1103 24-25 0.27166992076384844 1103 26-27 0.2641277729055247 1103 28-29 0.29873421862613725 1103 30-31 0.20268272166971002 1103 32-33 0.1460399215822079 1103 34-35 0.1346209539551282 1103 36-37 0.15535408965118336 1103 38-39 0.1879933416311843 1103 40-41 0.1403027211929384 1103 42-43 0.2496391843222341 1103 44-45 0.20036647178910272 1103 46-47 0.1587088865818842 1103 48-49 0.1534213197071992 1103 50-51 0.05022506535591731 1103 52-53 -0.008569356717060828 1103 54-55 0.10028873336869992 1103 56-57 0.05418568166779636 1103 58-59 0.05813090613224858 1103 60-61 0.05045378772199882 1103 62-63 0.0026595897049688233 1103 64-65 0.03745701689195613 1103 66-67 0.016472680934171535 1103 68-69 0.0047676159976006716 1103 70-71 0.02649473325359253 1103 72-73 -0.011840296704377806 1103 74-75 0.007665129318198183 1103 76-77 -9.691262542048662E-4 1103 78-79 -0.033862261804500804 1103 80-81 0.017571351342759556 1103 82-83 0.07278330978576264 1103 84-85 0.039833197644746576 1103 86-87 0.020632355966583305 1103 88-89 0.06801131284306194 1103 90-91 0.03319946774868754 1103 92-93 0.11073177561882375 1103 94-95 0.14104342907955925 1103 96-97 0.09184822560182937 1103 98-99 0.25020783318033324 1103 100 0.2155942898689105 1104 1 0.11586245449316834 1104 2 0.09333397400246213 1104 3 0.12967537326668577 1104 4 0.06667270729262498 1104 5 0.11515694422209322 1104 6 0.08930675870200844 1104 7 0.12013711845307995 1104 8 0.13410554731983382 1104 9 0.15856462052816056 1104 10-11 0.18024525689251192 1104 12-13 0.20747402223950218 1104 14-15 0.22224894116369853 1104 16-17 0.22878646430395122 1104 18-19 0.2080620773699593 1104 20-21 0.2160121752274975 1104 22-23 0.2731600586636418 1104 24-25 0.2549450608077066 1104 26-27 0.2135097886686026 1104 28-29 0.2836557560688675 1104 30-31 0.32174222266846897 1104 32-33 0.20182575489744892 1104 34-35 0.19129495302149735 1104 36-37 0.25301212073223667 1104 38-39 0.26093505651708426 1104 40-41 0.2495303155325601 1104 42-43 0.23527892149574114 1104 44-45 0.2111242013416188 1104 46-47 0.2717554579499719 1104 48-49 0.21627600927664759 1104 50-51 0.34426882499072065 1104 52-53 0.35363060745776664 1104 54-55 0.2982455450368491 1104 56-57 0.3239148012913091 1104 58-59 0.3337859368550511 1104 60-61 0.31978615033789026 1104 62-63 0.27198381023605833 1104 64-65 0.2647914883078073 1104 66-67 0.29396561767559604 1104 68-69 0.35223329032506 1104 70-71 0.2633218577949137 1104 72-73 0.2660058029656014 1104 74-75 0.2529430593831137 1104 76-77 0.21269936469180095 1104 78-79 0.24085100110885094 1104 80-81 0.2693042583927152 1104 82-83 0.2673475861172676 1104 84-85 0.23660143455770566 1104 86-87 0.23336281089200384 1104 88-89 0.24157833984221355 1104 90-91 0.277463199374715 1104 92-93 0.3016599325765057 1104 94-95 0.3339662271884265 1104 96-97 0.3824400805315875 1104 98-99 0.33472504826165306 1104 100 0.3504170366826429 1105 1 0.06829263000727792 1105 2 0.08099170904217345 1105 3 0.06286590527031422 1105 4 0.023712903330185497 1105 5 0.03746255070118565 1105 6 0.07645140749766455 1105 7 0.08283814792172706 1105 8 0.09095597173288184 1105 9 0.03517396307653087 1105 10-11 0.11695080533332458 1105 12-13 0.11335460781867113 1105 14-15 0.17283068127174062 1105 16-17 0.1512480729145551 1105 18-19 0.16025663392157696 1105 20-21 0.1528745740765558 1105 22-23 0.14545862040638724 1105 24-25 0.159216828962208 1105 26-27 0.14689635338694984 1105 28-29 0.20497627089044812 1105 30-31 0.16105045379657668 1105 32-33 0.15206769958075483 1105 34-35 0.04467932676221409 1105 36-37 0.15232231824661113 1105 38-39 0.13896910004386598 1105 40-41 0.1394137752348925 1105 42-43 0.16024197702409282 1105 44-45 0.10053016202875398 1105 46-47 0.16090540063878223 1105 48-49 0.11427404385984374 1105 50-51 0.12634313791080132 1105 52-53 0.1631711112986025 1105 54-55 0.15948338599966405 1105 56-57 0.16565702113943104 1105 58-59 0.1434496831876686 1105 60-61 0.08388701415827882 1105 62-63 0.10481754896488127 1105 64-65 0.09336784727138081 1105 66-67 0.0754718275460533 1105 68-69 0.07423086732354278 1105 70-71 0.14977529031038017 1105 72-73 0.14416866337415257 1105 74-75 0.16028260016325646 1105 76-77 0.13988474369699588 1105 78-79 0.14596502727584237 1105 80-81 0.149526613538562 1105 82-83 0.20650917847326866 1105 84-85 0.15083338541000302 1105 86-87 0.11779015461599585 1105 88-89 0.1505386547096137 1105 90-91 0.12701930019716912 1105 92-93 0.14384212529270002 1105 94-95 0.11644440062401173 1105 96-97 0.12571191299354467 1105 98-99 0.10711042506940771 1105 100 0.11724983632872465 1106 1 -0.008311618245045338 1106 2 0.0338600547609218 1106 3 -0.0033133081822214194 1106 4 0.032376409724079735 1106 5 -2.5977094901463715E-4 1106 6 0.020915178691311098 1106 7 0.044356960299971604 1106 8 0.054714591346602504 1106 9 -0.007568656317374689 1106 10-11 0.04221449903368324 1106 12-13 0.06499000007949007 1106 14-15 0.125682960174899 1106 16-17 0.09875941631922558 1106 18-19 0.08346156312745734 1106 20-21 0.041753450312242535 1106 22-23 0.0395641016121715 1106 24-25 0.10904505374673334 1106 26-27 0.0999049926766844 1106 28-29 0.0807886347009017 1106 30-31 0.0781278720968217 1106 32-33 0.10721951235997551 1106 34-35 0.07872560757382274 1106 36-37 0.017560272442679548 1106 38-39 0.05005953386185524 1106 40-41 0.07795762343747725 1106 42-43 0.14199263587995858 1106 44-45 0.11333145557040325 1106 46-47 0.1295151507276131 1106 48-49 0.20968147268966675 1106 50-51 0.2191908203075812 1106 52-53 0.24436767458345798 1106 54-55 0.2453376958414566 1106 56-57 0.220887631170811 1106 58-59 0.13535713087853907 1106 60-61 0.11762138918011544 1106 62-63 0.20139028763043854 1106 64-65 0.1923061334683993 1106 66-67 0.2454955272633228 1106 68-69 0.21915761894408092 1106 70-71 0.22744996104544413 1106 72-73 0.22927959192898584 1106 74-75 0.3317374107308382 1106 76-77 0.33058026450166267 1106 78-79 0.2858426074550735 1106 80-81 0.35148159571836146 1106 82-83 0.3605541972248716 1106 84-85 0.3013279871928134 1106 86-87 0.2628535905953271 1106 88-89 0.22850543878045926 1106 90-91 0.25291677155981773 1106 92-93 0.300364227875356 1106 94-95 0.2531236366632328 1106 96-97 0.22826267132395373 1106 98-99 0.23523210255672922 1106 100 0.22215380101339832 1107 1 -0.01654280173030287 1107 2 -0.002460505767963639 1107 3 0.06031931291994397 1107 4 -0.017778440685852104 1107 5 1.418923886475909E-4 1107 6 -0.0050835160149560465 1107 7 0.007928920820667429 1107 8 -0.01590416153283769 1107 9 0.006248592394896946 1107 10-11 0.038507521631771624 1107 12-13 0.02979976233725523 1107 14-15 0.0681983891594129 1107 16-17 0.013578386787472141 1107 18-19 0.04152623645572362 1107 20-21 0.06428015636493711 1107 22-23 0.07987640644221017 1107 24-25 0.12785827984627218 1107 26-27 0.10475873609827602 1107 28-29 0.09423082821622586 1107 30-31 0.05558375116550707 1107 32-33 0.009585648747325592 1107 34-35 0.07104295539394201 1107 36-37 0.07822601111307392 1107 38-39 0.035658400893197495 1107 40-41 0.08166930958339691 1107 42-43 0.07391234446999562 1107 44-45 0.020734378459714264 1107 46-47 -0.007154051186617494 1107 48-49 0.04728533786592237 1107 50-51 0.12701177502560057 1107 52-53 0.08335023010702969 1107 54-55 0.03714633487690833 1107 56-57 0.013903278252385576 1107 58-59 0.031553243040519874 1107 60-61 0.02289379226294841 1107 62-63 0.05469004089224683 1107 64-65 -0.03696649862382628 1107 66-67 -0.030631200434648065 1107 68-69 -0.025696247054334265 1107 70-71 -0.04328689909230121 1107 72-73 0.027726364137286907 1107 74-75 -0.034010094158034576 1107 76-77 -0.05204315466833975 1107 78-79 -0.0045593877369256575 1107 80-81 -0.04686110577328151 1107 82-83 -0.09038255881225155 1107 84-85 -0.10546182733050813 1107 86-87 -0.08063852158579721 1107 88-89 -0.07854921643334478 1107 90-91 -0.09589353063392281 1107 92-93 -0.05747729583234218 1107 94-95 -0.1223985480983174 1107 96-97 -0.1527248703858035 1107 98-99 -0.10832817429947994 1107 100 -0.07473953412570467 1108 1 -0.023541596796967212 1108 2 0.005966734477098612 1108 3 -0.00794246277941113 1108 4 -0.016332978290897415 1108 5 -0.006368980468721475 1108 6 -0.015505631646796303 1108 7 -0.028556134488148643 1108 8 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2214 52-53 -0.30403855048206196 2214 54-55 -0.2523161327725063 2214 56-57 -0.27585118866750946 2214 58-59 -0.26999333705922623 2214 60-61 -0.24153663245809298 2214 62-63 -0.205353705356778 2214 64-65 -0.21887554403801346 2214 66-67 -0.21612873828821932 2214 68-69 -0.22227972306362886 2214 70-71 -0.14822050033880174 2214 72-73 -0.17323889949823013 2214 74-75 -0.213410956082825 2214 76-77 -0.20471412443786363 2214 78-79 -0.20687818505585653 2214 80-81 -0.25102880056935106 2214 82-83 -0.21247605537573122 2214 84-85 -0.21124618331339917 2214 86-87 -0.2909006835415724 2214 88-89 -0.19347989579391367 2214 90-91 -0.21742637850083923 2214 92-93 -0.2770037294339076 2214 94-95 -0.331001956039664 2214 96-97 -0.2725961875000351 2214 98-99 -0.2446019051565571 2214 100 -0.24640222605984263 2215 1 -0.1606195356263811 2215 2 -0.1230447808446371 2215 3 -0.139465280511736 2215 4 -0.0894580608446276 2215 5 -0.13619017802594158 2215 6 -0.09044391602646584 2215 7 -0.1883118517364295 2215 8 -0.15331399630326814 2215 9 -0.2324033048361258 2215 10-11 -0.19441893533738863 2215 12-13 -0.18271683694069196 2215 14-15 -0.28792659200409787 2215 16-17 -0.2449104095838237 2215 18-19 -0.2628663604248018 2215 20-21 -0.2841328356210795 2215 22-23 -0.25013869688906 2215 24-25 -0.3200568069527705 2215 26-27 -0.3088461369571789 2215 28-29 -0.3190813172127065 2215 30-31 -0.4114155065913465 2215 32-33 -0.32024870960005103 2215 34-35 -0.22360404264218658 2215 36-37 -0.3612644696917542 2215 38-39 -0.25608594852482724 2215 40-41 -0.2904832101662649 2215 42-43 -0.3132300608757177 2215 44-45 -0.24367041764043762 2215 46-47 -0.27206401884880904 2215 48-49 -0.28920677567874975 2215 50-51 -0.24525150817624564 2215 52-53 -0.2674700534181511 2215 54-55 -0.32213122894648905 2215 56-57 -0.31529704010262094 2215 58-59 -0.3186921921885215 2215 60-61 -0.2936516340416162 2215 62-63 -0.31553646274751657 2215 64-65 -0.3759407620011501 2215 66-67 -0.3536743631902368 2215 68-69 -0.325266214199587 2215 70-71 -0.25970698042693385 2215 72-73 -0.2664295439864759 2215 74-75 -0.30110759428117007 2215 76-77 -0.26140595209594863 2215 78-79 -0.2933435427477207 2215 80-81 -0.27250491148114264 2215 82-83 -0.30000839657897416 2215 84-85 -0.33453637399892244 2215 86-87 -0.25818245999033707 2215 88-89 -0.2600914899114173 2215 90-91 -0.22239490271084605 2215 92-93 -0.19649591335815586 2215 94-95 -0.15888349280315595 2215 96-97 -0.21837449650885787 2215 98-99 -0.22971229926942982 2215 100 -0.14103277088614874 2216 1 -0.19744485392570255 2216 2 -0.13694609606729813 2216 3 -0.10790707088621332 2216 4 -0.15589752539926138 2216 5 -0.1675932290368678 2216 6 -0.14363388747806027 2216 7 -0.10556893981467397 2216 8 -0.14819649094191334 2216 9 -0.13735422910195894 2216 10-11 -0.19445343668761694 2216 12-13 -0.22772121685786573 2216 14-15 -0.31823880583294084 2216 16-17 -0.28816082467210435 2216 18-19 -0.27189255689069114 2216 20-21 -0.2882233410444215 2216 22-23 -0.25685963788317423 2216 24-25 -0.30485496625701103 2216 26-27 -0.2951103112618938 2216 28-29 -0.2920526280729945 2216 30-31 -0.29395142015786035 2216 32-33 -0.27581774407295256 2216 34-35 -0.2560499310753457 2216 36-37 -0.24964509716410532 2216 38-39 -0.22564688863009508 2216 40-41 -0.22328637275105478 2216 42-43 -0.24138124267366123 2216 44-45 -0.32485321554010227 2216 46-47 -0.23033658497347176 2216 48-49 -0.3433698508912997 2216 50-51 -0.35455846181617545 2216 52-53 -0.3354800603691004 2216 54-55 -0.29476927040813905 2216 56-57 -0.3020467673080489 2216 58-59 -0.26136171759418403 2216 60-61 -0.24820512029585018 2216 62-63 -0.29110185606095484 2216 64-65 -0.3600095686091933 2216 66-67 -0.33385372732840324 2216 68-69 -0.23114867492164848 2216 70-71 -0.25180422199525054 2216 72-73 -0.2704031444109347 2216 74-75 -0.34641473816583357 2216 76-77 -0.2868347142147911 2216 78-79 -0.3662159497373523 2216 80-81 -0.27493399171412847 2216 82-83 -0.2896636930040444 2216 84-85 -0.3702003417633577 2216 86-87 -0.3485269257593444 2216 88-89 -0.439879829000823 2216 90-91 -0.43790602754452834 2216 92-93 -0.4017875725690274 2216 94-95 -0.20845896051086754 2216 96-97 -0.361953132493106 2216 98-99 -0.4582444188569603 2216 100 -0.44167792664317673 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 21.0 3 5.0 4 8.0 5 21.0 6 47.0 7 101.0 8 288.0 9 529.0 10 989.0 11 1615.0 12 2360.0 13 3682.0 14 4614.0 15 6128.0 16 7496.0 17 10400.0 18 11862.0 19 6435.0 20 4624.0 21 4304.0 22 4469.0 23 4871.0 24 5752.0 25 7111.0 26 9123.0 27 12065.0 28 15563.0 29 20040.0 30 25917.0 31 33510.0 32 43012.0 33 55961.0 34 78106.0 35 117363.0 36 211395.0 37 469544.0 38 847446.0 39 409219.0 40 1444.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.735105880402116 19.850713004246874 13.670480930527695 23.74370018482331 2 15.637861097779199 19.954522068394564 40.52857710993461 23.879039723891623 3 18.4022673535794 24.947043525032424 30.050558407207085 26.60013071418109 4 12.157763902274114 16.7093147626415 39.07587176277566 32.05704957230873 5 12.422705535990337 37.7510839276747 36.205769022479394 13.620441513855566 6 31.082283051069975 38.492434685571745 17.273779046868846 13.151503216489433 7 27.19353091768413 32.429023893921496 22.37371996849153 18.00372521990285 8 25.02254423716784 36.71309535043058 19.956716705710523 18.307643706691064 9 24.873555861887883 16.322247932256793 21.342802284363923 37.4613939214914 10-11 24.01844159307466 26.61460352626235 28.88144250592326 20.485512374739738 12-13 24.80735936064067 23.88352123539451 29.204513752133387 22.10460565183143 14-15 21.84253048445894 25.948698844951686 26.571773020810234 25.636997649779143 16-17 21.057200657904186 29.475797733438853 27.052473561367947 22.414528047289018 18-19 20.764099735356556 28.015075711902348 29.86766186769223 21.353162685048865 20-21 21.979905146383093 27.250886175659712 29.575107489825392 21.19410118813181 22-23 21.52303107097603 27.07050094248648 29.75200779671913 21.654460189818355 24-25 21.446167700723002 27.208273544739647 29.620857406896466 21.724701347640885 26-27 21.246386574755952 27.162619110721998 29.69525788963658 21.895736424885474 28-29 21.22365904656759 27.249685171156322 29.743699884571306 21.78295589770478 30-31 21.536395372355983 27.745002028768724 29.319359376788505 21.39924322208679 32-33 21.09967835105685 27.365186425101744 29.93780359721675 21.597331626624655 34-35 21.301693580149667 27.131088354995402 29.877514933700933 21.689703131153998 36-37 21.65961008270973 27.592638177760275 29.182502953918863 21.565248785611132 38-39 21.538253249310753 27.72775945254037 29.257868911645 21.476118386503874 40-41 21.274588056273007 27.484919106676948 29.4908774108557 21.749615426194342 42-43 21.386864907443876 27.38442792437968 29.337214454509645 21.8914927136668 44-45 21.516221937770776 27.621992746488118 29.161661415255352 21.700123900485753 46-47 21.58857243665485 27.907353616909546 28.85221790074833 21.65185604568728 48-49 21.64666617434686 27.753319056058817 28.547369371143493 22.052645398450835 50-51 21.687631285282922 27.744211139556256 28.487634567414993 22.080523007745832 52-53 21.69848283444926 27.751165156885914 28.560108146251807 21.990243862413024 54-55 21.82078328081922 28.199463371406065 28.24442858080609 21.735324766968624 56-57 21.580408133123278 27.936974858868318 28.33577441906262 22.146842588945777 58-59 21.460138505973482 27.50939510305895 28.861736083760007 22.16873030720756 60-61 21.72796048312984 27.918102599448602 28.382052481291847 21.97188443612971 62-63 21.55041765130629 27.871209137455693 28.503245208087176 22.075128003150844 64-65 21.547607325718786 27.628844197190496 28.634366384403307 22.18918209268741 66-67 21.44696895103059 27.716723283444928 28.454382466850465 22.38192529867402 68-69 21.976533518446036 27.760177301211026 28.39363035060642 21.86965882973652 70-71 21.89272351319417 27.63600334777471 28.444802743862414 22.0264703951687 72-73 21.670794986117563 27.81283173995587 28.454634128329708 22.061739145596853 74-75 21.765677369911078 27.84665131038178 28.310704044430253 22.076967275276893 76-77 21.73495552711041 27.882409413154786 28.173985821189447 22.20864923854536 78-79 21.940683668110804 27.952770119469605 28.217043291322042 21.889502921097545 80-81 21.76933586057503 27.771227189182092 28.304491597741894 22.154945352500985 82-83 21.626986691151387 27.843369176288963 28.448471622976335 22.08117250958332 84-85 21.6438353354339 27.69861001706709 28.26682913220428 22.390725515294736 86-87 21.78855707972562 27.90165947431894 28.166180054102103 22.14360339185334 88-89 21.491383273402214 27.809577614337357 28.287405972351888 22.411633139908545 90-91 21.487569059640123 27.665109260494646 28.396999900713304 22.450321779151928 92-93 21.58611174246931 27.890569806016675 28.175481019731468 22.347837431782544 94-95 21.37890545189885 27.62293091154357 28.354789027441242 22.643374609116336 96-97 21.323233929075105 27.633549578194312 28.377055821944154 22.666160670786432 98-99 21.283876232389815 27.671830966846763 28.289097696393632 22.75519510436979 100 21.57534134173559 27.494502428777736 28.17505251411317 22.755103715373508 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 14.0 1 13.5 2 54.5 3 90.0 4 61.0 5 48.0 6 124.5 7 312.0 8 583.5 9 697.0 10 594.5 11 556.5 12 626.5 13 732.5 14 878.5 15 1048.5 16 1218.5 17 1407.5 18 1716.0 19 2087.5 20 2594.0 21 3350.5 22 4437.0 23 6133.0 24 8282.5 25 10803.5 26 14363.0 27 18831.5 28 24598.5 29 31448.5 30 38051.0 31 45208.0 32 52882.0 33 60324.5 34 68311.0 35 75386.5 36 79959.5 37 84255.0 38 88309.0 39 89990.5 40 90416.0 41 90784.5 42 89654.5 43 88467.5 44 87310.5 45 85355.5 46 83924.0 47 81634.0 48 78950.5 49 76489.0 50 73952.0 51 70726.0 52 65659.0 53 61731.5 54 59458.5 55 57062.5 56 53897.0 57 49468.5 58 45288.0 59 41650.0 60 36892.5 61 31245.0 62 26501.0 63 22071.0 64 17553.5 65 13676.5 66 10422.0 67 7550.0 68 5532.5 69 4194.5 70 2968.5 71 2071.0 72 1468.5 73 1027.5 74 722.5 75 494.0 76 331.5 77 201.5 78 122.5 79 78.5 80 34.0 81 11.5 82 5.5 83 2.5 84 0.5 85 1.5 86 2.0 87 0.5 88 0.0 89 0.0 90 0.5 91 0.5 92 0.0 93 0.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04344722331626624 2 0.24332906656163844 3 0.0025026257056583955 4 0.0020923591965340683 5 3.282132072994617E-4 6 0.0 7 0.0 8 4.102665091243272E-4 9 0.0 10-11 6.153997636864908E-4 12-13 0.0 14-15 3.897531836681108E-4 16-17 1.2307995273729813E-4 18-19 3.897531836681108E-4 20-21 0.0 22-23 1.8461992910594722E-4 24-25 0.0013333661546540633 26-27 8.205330182486543E-5 28-29 1.8461992910594722E-4 30-31 4.1026650912432715E-5 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 6.153997636864907E-5 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 2.4615990547459626E-4 70-71 0.0 72-73 3.076998818432454E-4 74-75 6.153997636864907E-5 76-77 0.0 78-79 0.0 80-81 0.0 82-83 6.153997636864907E-5 84-85 0.0 86-87 4.7180648549297624E-4 88-89 0.002400059078377314 90-91 0.0022154391492713666 92-93 5.74373112774058E-4 94-95 5.74373112774058E-4 96-97 1.025666272810818E-4 98-99 4.1026650912432715E-5 100 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2437440.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 63.25683499537361 #Duplication Level Percentage of deduplicated Percentage of total 1 74.72965903332852 47.27161710731792 2 15.091275528644907 19.092526519704208 3 4.964914332040108 9.421943000040804 4 2.104296724552446 5.324446027452769 5 1.0274980159746105 3.249813622728985 6 0.6036974621050581 2.2912794448503284 7 0.3699178691296477 1.6379883526562036 8 0.23590446803144516 1.1938056007149211 9 0.16920841628394495 0.9633229981831797 >10 0.6663017651111207 7.056085530501733 >50 0.028271928968665056 1.2127751371511208 >100 0.008794651171043067 0.9271123589551766 >500 0.0 0.0 >1k 2.598046584618153E-4 0.3572842997427152 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3276 0.13440330838912956 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2863 0.11745930156229487 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 2.0513325456216357E-5 0.0 26-27 0.0 0.0 0.0 8.205330182486543E-5 0.0 28-29 0.0 0.0 0.0 3.897531836681108E-4 0.0 30-31 0.0 0.0 0.0 0.0018256859656032558 0.0 32-33 0.0 0.0 0.0 0.005210384665878955 0.0 34-35 0.0 0.0 0.0 0.010154096100827098 0.0 36-37 0.0 0.0 0.0 0.019631252461599052 0.0 38-39 0.0 0.0 0.0 0.037990678744912694 0.0 40-41 0.0 0.0 0.0 0.06199126952868583 0.0 42-43 0.0 0.0 0.0 0.0867713666797952 0.0 44-45 0.0 0.0 0.0 0.11345920309833268 0.0 46-47 0.0 0.0 0.0 0.1483728830248129 0.0 48-49 0.0 0.0 0.0 0.18771744124983591 0.0 50-51 0.0 0.0 0.0 0.23420063673362215 0.0 52-53 0.0 0.0 0.0 0.28320697124852307 0.0 54-55 0.0 0.0 0.0 0.3343261782854142 0.0 56-57 0.0 0.0 0.0 0.39668668767231197 0.0 58-59 0.0 0.0 0.0 0.4704731193383222 0.0 60-61 0.0 0.0 0.0 0.5524033412104503 0.0 62-63 0.0 0.0 0.0 0.6392772745175266 0.0 64-65 0.0 0.0 0.0 0.7291461533412105 0.0 66-67 0.0 0.0 0.0 0.8318153472495733 0.0 68-69 0.0 0.0 0.0 0.9483925758172509 0.0 70-71 0.0 0.0 0.0 1.0794522121570171 0.0 72-73 0.0 0.0 0.0 1.2195171983720625 0.0 74-75 0.0 0.0 0.0 1.355910299330445 0.0 76-77 0.0 0.0 0.0 1.5044472889589078 0.0 78-79 0.0 0.0 0.0 1.6786054220821844 0.0 80-81 0.0 0.0 0.0 1.874343573585401 0.0 82-83 0.0 0.0 0.0 2.086656492057241 0.0 84-85 0.0 0.0 0.0 2.293389786004989 0.0 86-87 0.0 0.0 0.0 2.5130259616646975 0.0 88 0.0 0.0 0.0 2.694179138768544 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3090 0.0 55.529346 1 GTATCAA 5930 0.0 41.73345 1 TCAACGC 6705 0.0 36.167545 4 ATCAACG 6840 0.0 35.38645 3 CAACGCA 6865 0.0 35.3246 5 AACGCAG 7155 0.0 33.892857 6 TATCAAC 7325 0.0 33.191376 2 ACGCAGA 8155 0.0 29.333366 7 CGCAGAG 8400 0.0 28.47781 8 GCAGAGT 9535 0.0 24.693642 9 GTACATG 6185 0.0 24.541203 1 TACATGG 6445 0.0 22.955664 2 GTGGTAT 1590 0.0 22.235344 1 ACATGGG 6485 0.0 21.959303 3 GAGTACT 6340 0.0 21.867586 12-13 AGAGTAC 8615 0.0 20.975348 10-11 GAGTACA 4600 0.0 20.700136 1 CAGAGTA 9410 0.0 20.526756 10-11 CGTACAC 215 1.1954344E-6 19.67388 3 TATACTG 1720 0.0 19.67307 5 >>END_MODULE