FastQCFastQC Report
Wed 25 May 2016
SRR1033081_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033081_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2125358
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA15350.042.2614631
TCAACGC26600.034.4609154
GTATCAA28150.033.7323651
ATCAACG27550.033.272613
CAACGCA28550.032.1071935
AACGCAG29350.031.066756
GTACATG59150.028.6102471
TACATGG58850.026.9987752
TATCAAC34550.026.3953552
ACATGGG60400.025.3719883
ACGCAGA35700.025.278167
CGCAGAG35800.025.1022788
TATACCG1351.4562231E-524.3746345
CATGGGG41250.021.994154
GCAGAGT44350.019.94759
GAGTACA42500.019.2457181
CTAGTAC4250.018.8032883
TGTACGG1350.00998347617.4104545
CTGTCGC5200.017.1939059
AGAGTAC38100.016.43331110-11
Produced by FastQC (version 0.11.5)