##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1033081_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2125358 Sequences flagged as poor quality 0 Sequence length 100 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.251524684312006 33.0 31.0 34.0 28.0 34.0 2 31.274531161338466 33.0 31.0 34.0 28.0 34.0 3 31.467309036877552 34.0 31.0 34.0 28.0 34.0 4 34.88014725048674 37.0 35.0 37.0 32.0 37.0 5 34.92178023655309 37.0 35.0 37.0 32.0 37.0 6 34.99506436092178 37.0 35.0 37.0 32.0 37.0 7 34.85556927350592 37.0 35.0 37.0 32.0 37.0 8 34.83954985465978 37.0 35.0 37.0 32.0 37.0 9 36.417017274266264 39.0 37.0 39.0 32.0 39.0 10-11 36.46462925304819 39.0 37.0 39.0 32.0 39.0 12-13 36.420579497665805 39.0 37.0 39.0 32.0 39.0 14-15 37.74562850117486 40.0 38.0 41.0 32.5 41.0 16-17 37.72514865730855 40.0 38.0 41.0 32.0 41.0 18-19 37.710021793975415 40.0 38.0 41.0 32.0 41.0 20-21 37.759240090375364 40.0 38.0 41.0 32.0 41.0 22-23 37.690387454725276 40.0 38.0 41.0 32.0 41.0 24-25 37.70329092792838 40.0 38.0 41.0 32.0 41.0 26-27 37.62694143763074 40.0 38.0 41.0 32.0 41.0 28-29 37.641420410114435 40.0 38.0 41.0 32.0 41.0 30-31 37.797207576323615 40.0 38.0 41.0 32.0 41.0 32-33 37.79128033959455 40.0 38.0 41.0 32.0 41.0 34-35 37.741230653847495 40.0 38.0 41.0 32.0 41.0 36-37 37.64836935706832 40.0 38.0 41.0 31.5 41.0 38-39 37.54704242767571 40.0 38.0 41.0 31.0 41.0 40-41 37.47525405131748 40.0 38.0 41.0 31.0 41.0 42-43 37.32487303315489 40.0 38.0 41.0 31.0 41.0 44-45 37.149931917352276 40.0 37.5 41.0 30.0 41.0 46-47 36.99667679515639 40.0 37.0 41.0 30.0 41.0 48-49 36.86560758234613 40.0 37.0 41.0 30.0 41.0 50-51 36.73415513998113 40.0 36.5 41.0 30.0 41.0 52-53 36.56878300032277 40.0 36.0 41.0 30.0 41.0 54-55 36.40600477660705 40.0 36.0 41.0 30.0 41.0 56-57 36.229816576783776 39.0 35.5 41.0 29.5 41.0 58-59 36.027020859544606 39.0 35.0 41.0 29.0 41.0 60-61 35.79920888622058 39.0 35.0 41.0 29.0 41.0 62-63 35.54487549862188 38.5 35.0 40.0 28.5 41.0 64-65 35.26432888012278 38.0 34.5 40.0 28.0 41.0 66-67 34.96998905596139 37.5 34.0 40.0 28.0 41.0 68-69 34.65431165008437 37.0 34.0 40.0 28.0 41.0 70-71 34.318855458703894 36.5 34.0 39.5 27.5 41.0 72-73 33.968064674280754 36.0 34.0 39.0 27.0 41.0 74-75 33.61716567279489 35.5 33.5 39.0 26.0 40.0 76-77 33.26127551217253 35.0 33.0 38.0 26.0 40.0 78-79 32.8698433393339 35.0 33.0 37.0 26.0 39.0 80-81 32.5137826192105 35.0 33.0 37.0 26.0 39.0 82-83 32.299282991383095 35.0 33.0 36.0 26.0 38.5 84-85 32.07209538346011 35.0 33.0 36.0 25.5 37.0 86-87 31.896484262886535 35.0 33.0 36.0 25.0 37.0 88-89 31.66599744607732 35.0 33.0 35.0 25.0 37.0 90-91 31.444159760379193 35.0 32.5 35.0 25.0 36.0 92-93 31.20738553222563 35.0 32.0 35.0 24.0 36.0 94-95 30.983590293964593 35.0 32.0 35.0 23.0 36.0 96-97 30.786877316668534 35.0 32.0 35.0 21.0 35.5 98-99 30.584056191945074 35.0 32.0 35.0 19.5 35.0 100 30.421111643309033 34.0 32.0 35.0 18.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.24227153212989805 1101 2 0.2692721792019164 1101 3 0.32324202042475747 1101 4 0.11393220708396257 1101 5 0.1672787470956294 1101 6 0.2532059837905649 1101 7 0.25928735831989513 1101 8 0.3613154186986449 1101 9 0.4823161779995715 1101 10-11 0.396554005810259 1101 12-13 0.4451654575122177 1101 14-15 0.5276108984694261 1101 16-17 0.5207977197929736 1101 18-19 0.5017139886862552 1101 20-21 0.5470723895037111 1101 22-23 0.5646518889400767 1101 24-25 0.5523662257629951 1101 26-27 0.43085546792377727 1101 28-29 0.43761067659810493 1101 30-31 0.5406889376684489 1101 32-33 0.4939625134471939 1101 34-35 0.5278568443434679 1101 36-37 0.5365661063748988 1101 38-39 0.555722971027194 1101 40-41 0.5381662137880525 1101 42-43 0.533051407270662 1101 44-45 0.5071192717237238 1101 46-47 0.545374852979883 1101 48-49 0.5292275552698698 1101 50-51 0.5472375123289055 1101 52-53 0.5293875899520373 1101 54-55 0.4451387511545519 1101 56-57 0.6118132129714482 1101 58-59 0.5897784435015296 1101 60-61 0.6223182116456201 1101 62-63 0.5648310077416312 1101 64-65 0.5309615369198468 1101 66-67 0.5267161696899194 1101 68-69 0.5807050824782252 1101 70-71 0.627136443812887 1101 72-73 0.49159871259905685 1101 74-75 0.5082445854761772 1101 76-77 0.5383010507587755 1101 78-79 0.541084331813984 1101 80-81 0.5499534125848768 1101 82-83 0.5166050117245078 1101 84-85 0.5419719109506929 1101 86-87 0.4806571954774377 1101 88-89 0.5098805228866041 1101 90-91 0.5425093537376604 1101 92-93 0.5513158412181909 1101 94-95 0.5680879528642926 1101 96-97 0.4448063084561902 1101 98-99 0.5120542836943223 1101 100 0.5470017160975402 1102 1 0.18998926362835178 1102 2 0.18708925791781184 1102 3 0.22458058708122408 1102 4 0.0830667953303319 1102 5 0.05962532664693043 1102 6 0.20226207218993864 1102 7 0.2067708627943361 1102 8 0.2734562793349653 1102 9 0.2506308085186788 1102 10-11 0.30611248437405436 1102 12-13 0.3154226807357077 1102 14-15 0.40028339851544814 1102 16-17 0.4253387874813299 1102 18-19 0.40644768188241187 1102 20-21 0.4316008555451134 1102 22-23 0.3406955451283693 1102 24-25 0.37357706760754894 1102 26-27 0.3709537710247375 1102 28-29 0.45175810537968886 1102 30-31 0.5092969812342076 1102 32-33 0.4875363027673174 1102 34-35 0.6104606750856547 1102 36-37 0.5557501153580233 1102 38-39 0.6164537267134875 1102 40-41 0.45254079617059517 1102 42-43 0.5205538223726904 1102 44-45 0.5269787518236342 1102 46-47 0.5486082929544907 1102 48-49 0.5042728799469813 1102 50-51 0.6062972737809176 1102 52-53 0.5887774833239803 1102 54-55 0.5324880097502742 1102 56-57 0.599701286947095 1102 58-59 0.5745605622609276 1102 60-61 0.5288370993166254 1102 62-63 0.5221017058943147 1102 64-65 0.5510856003322147 1102 66-67 0.5353646820919522 1102 68-69 0.6446692198736983 1102 70-71 0.4601862381475712 1102 72-73 0.5513392473218772 1102 74-75 0.599171224873075 1102 76-77 0.5626800053791001 1102 78-79 0.5118473805315773 1102 80-81 0.7075750317625022 1102 82-83 0.656136613611281 1102 84-85 0.5505436295174846 1102 86-87 0.6378198578719214 1102 88-89 0.6210542240161843 1102 90-91 0.6384659310772101 1102 92-93 0.6743710286009765 1102 94-95 0.7750166329495691 1102 96-97 0.7416986169301509 1102 98-99 0.7604666090233074 1102 100 0.7826440073832011 1103 1 0.26793220808213647 1103 2 0.3464303978809973 1103 3 0.27075289477284414 1103 4 0.2756920272877963 1103 5 0.2272104629609757 1103 6 0.2498512244756057 1103 7 0.3049574688603869 1103 8 0.2752457490428526 1103 9 0.4233648349748407 1103 10-11 0.3627780779606269 1103 12-13 0.4161081259697852 1103 14-15 0.4767350624971911 1103 16-17 0.5319933104025054 1103 18-19 0.543985405439372 1103 20-21 0.5938834941454445 1103 22-23 0.5785518274997514 1103 24-25 0.5771765771776316 1103 26-27 0.5090038541132316 1103 28-29 0.5307781414454311 1103 30-31 0.5393155890893979 1103 32-33 0.452047215033609 1103 34-35 0.4527276509509406 1103 36-37 0.4844528016352072 1103 38-39 0.5689058038812078 1103 40-41 0.5236086735568719 1103 42-43 0.48794338621745226 1103 44-45 0.5163138622578387 1103 46-47 0.4963940874466104 1103 48-49 0.5202700750069766 1103 50-51 0.4953108724803883 1103 52-53 0.52829415378487 1103 54-55 0.5028553430080578 1103 56-57 0.5531506711629248 1103 58-59 0.5581449439645425 1103 60-61 0.5397034560492386 1103 62-63 0.493129156130081 1103 64-65 0.5004728247955015 1103 66-67 0.5021013719707952 1103 68-69 0.4499779465778033 1103 70-71 0.4516621043756004 1103 72-73 0.4383199553920534 1103 74-75 0.3411252691871596 1103 76-77 0.3430157178747564 1103 78-79 0.40875998534492197 1103 80-81 0.46474714589855637 1103 82-83 0.507005669201618 1103 84-85 0.48507003180083785 1103 86-87 0.46651465483748566 1103 88-89 0.4717230938956902 1103 90-91 0.5988678268743364 1103 92-93 0.6353776601914056 1103 94-95 0.5428944203809785 1103 96-97 0.6137249565335914 1103 98-99 0.5694303267700356 1103 100 0.55712034596705 1104 1 0.12842066275432984 1104 2 0.1235822688311572 1104 3 0.15366575465297672 1104 4 0.1670510354150352 1104 5 0.1044508396170798 1104 6 0.13763343088596258 1104 7 0.15784720182853818 1104 8 0.2337806196884813 1104 9 0.23814077431867275 1104 10-11 0.23461963826558474 1104 12-13 0.29182430815941274 1104 14-15 0.3004502049852462 1104 16-17 0.3631453422336932 1104 18-19 0.3567212634209582 1104 20-21 0.3280191396837324 1104 22-23 0.31625280590375127 1104 24-25 0.31327191641620544 1104 26-27 0.2642537075286242 1104 28-29 0.2846072314623811 1104 30-31 0.27944410575892675 1104 32-33 0.2893493522460986 1104 34-35 0.28951062445303677 1104 36-37 0.336154847857955 1104 38-39 0.33707877994872604 1104 40-41 0.4337151837736073 1104 42-43 0.4435679698109425 1104 44-45 0.3987031188722767 1104 46-47 0.3450857980675224 1104 48-49 0.3695022486333315 1104 50-51 0.32934903806882687 1104 52-53 0.3324838678214803 1104 54-55 0.3174386373631606 1104 56-57 0.39265218520984035 1104 58-59 0.3348608400142581 1104 60-61 0.34599043714845834 1104 62-63 0.4397366329542791 1104 64-65 0.435269868939578 1104 66-67 0.4012648011974633 1104 68-69 0.4027890148542568 1104 70-71 0.26609852344299156 1104 72-73 0.2926024402787988 1104 74-75 0.40429353456939054 1104 76-77 0.2552611424109301 1104 78-79 0.3270252354552028 1104 80-81 0.365466427849114 1104 82-83 0.4021476012559191 1104 84-85 0.3410549804399352 1104 86-87 0.43657286692074493 1104 88-89 0.40733019832576645 1104 90-91 0.4016414284751981 1104 92-93 0.3206774575468465 1104 94-95 0.36119647651873166 1104 96-97 0.46078021536125036 1104 98-99 0.47412993430277695 1104 100 0.48273654831437085 1105 1 0.17731743012413403 1105 2 0.1500942640478975 1105 3 0.12997943980927218 1105 4 0.10106215260694285 1105 5 0.1777658117329537 1105 6 0.13675257044998546 1105 7 0.1563815991348747 1105 8 0.16738999241619723 1105 9 0.24798255161106653 1105 10-11 0.2720930696595474 1105 12-13 0.2766249590522847 1105 14-15 0.3046527234885801 1105 16-17 0.32219961125725405 1105 18-19 0.3347611737510263 1105 20-21 0.1861070345265432 1105 22-23 0.2741360713378498 1105 24-25 0.2075671450247043 1105 26-27 0.2916643869841451 1105 28-29 0.3952941101863914 1105 30-31 0.392092716778329 1105 32-33 0.2951096812410938 1105 34-35 0.2686717127420266 1105 36-37 0.31013336166876115 1105 38-39 0.350056280638384 1105 40-41 0.33167796362250357 1105 42-43 0.33517418407978994 1105 44-45 0.32139566556556787 1105 46-47 0.17109834197382412 1105 48-49 0.1506198739842901 1105 50-51 0.11340708452332393 1105 52-53 0.2725635341436856 1105 54-55 0.174269093300083 1105 56-57 0.2222952655842505 1105 58-59 0.17891198379604134 1105 60-61 0.20282499458546255 1105 62-63 0.09247796420601162 1105 64-65 0.10684098449494428 1105 66-67 0.04607028679684788 1105 68-69 0.12876323758454333 1105 70-71 0.023563608031111016 1105 72-73 0.08039811792561125 1105 74-75 0.1542877114519996 1105 76-77 0.11240437570547357 1105 78-79 0.1540202152191128 1105 80-81 0.1327454400377519 1105 82-83 0.12478760823960755 1105 84-85 0.09687430014589893 1105 86-87 -0.0084532591283768 1105 88-89 0.03843636985799748 1105 90-91 0.01683053902288023 1105 92-93 0.12301437076396837 1105 94-95 0.12510992939847299 1105 96-97 0.2378982198731059 1105 98-99 0.31824330948693813 1105 100 0.26122020044396876 1106 1 0.10145462560950591 1106 2 0.08736448607772118 1106 3 0.13825183884812375 1106 4 0.016618748567871933 1106 5 0.06114604173050964 1106 6 0.16177758723240032 1106 7 0.10968844134249878 1106 8 0.12129284574099586 1106 9 0.11548849603773448 1106 10-11 0.11912544404236058 1106 12-13 0.10372154579071946 1106 14-15 0.17598529455506906 1106 16-17 0.12915572758608107 1106 18-19 0.24419748055245805 1106 20-21 0.17799618586944632 1106 22-23 0.23784605232822997 1106 24-25 0.16312255680565357 1106 26-27 0.1851603372454491 1106 28-29 0.08604060626840493 1106 30-31 0.13367719664883282 1106 32-33 0.17111368549472417 1106 34-35 0.1278854564446945 1106 36-37 0.12815018957212487 1106 38-39 0.2277865605126621 1106 40-41 0.26151373119071764 1106 42-43 0.13040927125425839 1106 44-45 0.19775825677096037 1106 46-47 0.11068320454620562 1106 48-49 0.2337120580827161 1106 50-51 0.2994841079810655 1106 52-53 0.25284007545980813 1106 54-55 0.1640120164522969 1106 56-57 0.19182458933121893 1106 58-59 0.20925483134894307 1106 60-61 0.16683356930330007 1106 62-63 0.0792722007785116 1106 64-65 0.2044932888621318 1106 66-67 0.18077703364269126 1106 68-69 0.2506677048447088 1106 70-71 0.24261141753341064 1106 72-73 0.11956114578755717 1106 74-75 0.22284957583751464 1106 76-77 0.32499409106310395 1106 78-79 0.35518945571272553 1106 80-81 0.3552491337382264 1106 82-83 0.3369442352558494 1106 84-85 0.36376592179803424 1106 86-87 0.31658669714322585 1106 88-89 0.33108527853027425 1106 90-91 0.28877159038649225 1106 92-93 0.25892745195322675 1106 94-95 0.17138940842201933 1106 96-97 0.14201591167889305 1106 98-99 0.17879435313377456 1106 100 0.21286801433792846 1107 1 0.1533116055860333 1107 2 0.18271423641058604 1107 3 0.18296182188989007 1107 4 0.20787514206574542 1107 5 0.2360358207876132 1107 6 0.26749471034433725 1107 7 0.2614226183366881 1107 8 0.27389618926142134 1107 9 0.2343018522047231 1107 10-11 0.2864936590400049 1107 12-13 0.293009647166663 1107 14-15 0.3176640878027399 1107 16-17 0.2758326553518913 1107 18-19 0.32384800539239933 1107 20-21 0.367579338917146 1107 22-23 0.3169176230677522 1107 24-25 0.2982366282701179 1107 26-27 0.32276761668589415 1107 28-29 0.34246916495498425 1107 30-31 0.39763927517897457 1107 32-33 0.34421593249895466 1107 34-35 0.31392025612647956 1107 36-37 0.30853768412559646 1107 38-39 0.3733485165244872 1107 40-41 0.35610400457205316 1107 42-43 0.3349174653311735 1107 44-45 0.2437998864316242 1107 46-47 0.3005475082944784 1107 48-49 0.284358941911961 1107 50-51 0.30350257985726614 1107 52-53 0.2470189844698396 1107 54-55 0.20100079281764494 1107 56-57 0.17689039168958232 1107 58-59 0.19528070214752802 1107 60-61 0.19840656524819877 1107 62-63 0.22878388290799023 1107 64-65 0.2348109274771204 1107 66-67 0.3094020084026994 1107 68-69 0.29885506564522757 1107 70-71 0.26455151488005413 1107 72-73 0.2511999270111289 1107 74-75 0.19995704846826357 1107 76-77 0.2862489056383879 1107 78-79 0.3018156567945951 1107 80-81 0.31302429326254355 1107 82-83 0.2486748236170655 1107 84-85 0.22345184334987778 1107 86-87 0.2035199455335608 1107 88-89 0.14366137804490364 1107 90-91 0.12047684956511873 1107 92-93 0.06291317826521592 1107 94-95 0.10225195561890033 1107 96-97 0.03777034632592091 1107 98-99 0.08963466257224084 1107 100 0.11823627526096203 1108 1 0.09510567332001685 1108 2 0.19597052869751153 1108 3 0.13617914823506183 1108 4 0.18530642057423563 1108 5 0.23906175805505114 1108 6 0.29004070639975055 1108 7 0.2258188594041144 1108 8 0.21259432538477796 1108 9 0.31037143167234404 1108 10-11 0.32165627180702216 1108 12-13 0.4162780939258255 1108 14-15 0.41878686978881063 1108 16-17 0.39145862885276017 1108 18-19 0.3198142859357276 1108 20-21 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-1.1003504224822152 2215 66-67 -1.078508985493599 2215 68-69 -1.0357982584266665 2215 70-71 -1.0239279404799646 2215 72-73 -1.0906249874618439 2215 74-75 -1.0622271315523761 2215 76-77 -0.8883019806481087 2215 78-79 -0.9731369786994293 2215 80-81 -1.1314933530597493 2215 82-83 -1.1256926664760556 2215 84-85 -1.061788266710085 2215 86-87 -0.9533346460885461 2215 88-89 -1.0316578432728463 2215 90-91 -1.0357731876758116 2215 92-93 -1.046422223452609 2215 94-95 -1.0763587681644573 2215 96-97 -1.1075709383070524 2215 98-99 -1.0138582229599855 2215 100 -1.0231217193679214 2216 1 -1.3046167758151945 2216 2 -1.318409056844736 2216 3 -1.2000645964157393 2216 4 -1.3841201520572852 2216 5 -1.290980791672382 2216 6 -1.2864261368683358 2216 7 -1.3538130014292307 2216 8 -1.2593689755933752 2216 9 -1.3735743692266027 2216 10-11 -1.4448992096422302 2216 12-13 -1.6085039815882425 2216 14-15 -1.80048926189302 2216 16-17 -1.7671551384498372 2216 18-19 -1.8384751052788388 2216 20-21 -1.7017051022991083 2216 22-23 -1.7046859905418756 2216 24-25 -1.72776814498949 2216 26-27 -1.7177734659209065 2216 28-29 -1.6957335816705452 2216 30-31 -1.9717179234554578 2216 32-33 -1.943454768358805 2216 34-35 -2.197727264399056 2216 36-37 -2.2444906509786477 2216 38-39 -2.0934018007940978 2216 40-41 -2.0242964260082985 2216 42-43 -2.1458107474903017 2216 44-45 -1.9293382260785634 2216 46-47 -1.975771592669787 2216 48-49 -2.0648557292418346 2216 50-51 -1.9001097718558384 2216 52-53 -1.976616875516072 2216 54-55 -2.088965448127375 2216 56-57 -2.188509704176738 2216 58-59 -2.0871063111943187 2216 60-61 -2.028488600117683 2216 62-63 -2.0086810765792933 2216 64-65 -2.137260709594706 2216 66-67 -2.18819346052814 2216 68-69 -2.111889061267071 2216 70-71 -2.0266771389634357 2216 72-73 -2.0287396714006434 2216 74-75 -2.2375857529236924 2216 76-77 -2.100582784575991 2216 78-79 -2.102362929019705 2216 80-81 -2.069370390792475 2216 82-83 -2.239295395718713 2216 84-85 -2.138227076958543 2216 86-87 -2.1203464932753917 2216 88-89 -2.1751129211602347 2216 90-91 -2.2888998319770444 2216 92-93 -2.5147339237138375 2216 94-95 -2.445224480909072 2216 96-97 -2.4051655294148624 2216 98-99 -2.352636755233295 2216 100 -2.3442509024646228 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 29700.0 3 6160.0 4 3425.0 5 2946.0 6 2301.0 7 2388.0 8 2889.0 9 3275.0 10 3498.0 11 4005.0 12 4475.0 13 4848.0 14 5374.0 15 5394.0 16 5376.0 17 5565.0 18 5486.0 19 5399.0 20 5402.0 21 5712.0 22 6685.0 23 9876.0 24 15091.0 25 18279.0 26 17339.0 27 15774.0 28 17903.0 29 22380.0 30 26903.0 31 33885.0 32 43605.0 33 59011.0 34 84018.0 35 129178.0 36 230918.0 37 447287.0 38 623223.0 39 210145.0 40 240.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.79891790503703 20.11762556802453 13.511356724110982 23.57209980282746 2 15.293513467459544 20.231762408227763 40.7496739105364 23.725050213776296 3 17.889183498988743 25.63777960235169 30.07803819804076 26.394998700618803 4 11.997035373977967 17.060396250331966 39.61533318139782 31.327235194292246 5 12.455560929503587 38.443816629172 35.696682651938175 13.403939789386238 6 30.272971901281032 38.83444897482484 17.973836817770394 12.918742306123738 7 26.91460363866525 32.9296755793495 22.70308785466437 17.45263292732088 8 24.335772171621116 37.39368351245466 20.528443222356298 17.742101093567932 9 24.338360691562787 17.81613932233101 21.455703028707422 36.38979695739878 10-11 23.2375544389162 27.66600263336813 29.09994246719417 19.996500460521496 12-13 23.963206681335826 24.980635157416327 29.04594501727116 22.010213143976692 14-15 21.377700739038072 27.140949491999645 25.969180840591044 25.512168928371242 16-17 21.030242075033794 30.40758314369353 26.504558182810417 22.057616598462257 18-19 20.883685267014002 29.27767327203656 28.495913286505132 21.34272817444431 20-21 22.29737764481351 28.582011441677235 27.960407416957185 21.160203496552075 22-23 21.95068212483605 28.279792175313943 28.0208116574659 21.74871404238411 24-25 21.752248230852096 28.341121176454532 27.993386019907824 21.913244572785548 26-27 21.486101136934376 28.21729755184012 28.19627420012864 22.100327111096867 28-29 21.59161880171539 28.249796546002027 28.208317119397424 21.950267532885157 30-31 21.870857056485647 28.763749775452606 27.768325009482197 21.597068158579546 32-33 21.431369576251473 28.399339125947453 28.371324017564298 21.797967280236776 34-35 21.549029283045755 28.070587842195753 28.422369350818403 21.958013523940085 36-37 21.97086532178642 28.567807046780818 27.71777098621413 21.743556645218632 38-39 21.735278440147518 28.674998399011532 27.982971133236145 21.60675202760481 40-41 21.40964281787293 28.33535154424332 28.37594336546225 21.879062272421507 42-43 21.41333193954736 28.20542521773197 28.31290212608866 22.068340716632008 44-45 21.613370672900913 28.464992978230754 28.202226954412062 21.719409394456274 46-47 21.637610989516034 28.67781678865939 27.995257164168418 21.68931505765616 48-49 21.499051653343983 28.405572172288313 28.206273402596228 21.889102771771476 50-51 21.59034406382546 28.213167767841675 28.19873603441392 21.99775213391895 52-53 21.54728898699821 28.237615529065202 28.25122380289569 21.963871681040896 54-55 21.750575769853082 28.73213394915145 27.90777606525368 21.609514215741783 56-57 21.52794322741522 28.41237719190034 28.00050568733456 22.059173893349882 58-59 21.342330005252137 27.958871818848046 28.488370313713496 22.210427862186314 60-61 21.72833135629988 28.521707074028935 27.942674426022734 21.80728714364845 62-63 21.48495528136602 28.491361146228407 27.99052186257407 22.033161709831507 64-65 21.51243063845695 28.132884133647813 28.21214774247025 22.14253748542498 66-67 21.393566448975616 28.271798591399754 28.002880575022665 22.331754384601965 68-69 21.871056030875398 28.392363734211468 27.972733070404022 21.76384716450911 70-71 21.771646295962377 28.118586829764126 28.112183958264573 21.99758291600892 72-73 21.487156828658613 28.34077934942244 28.271313539383065 21.90075028253588 74-75 21.570038017443295 28.335895293134495 28.113369663728065 21.980697025694145 76-77 21.6152380755101 28.344286463164973 27.915851527675024 22.124623933649904 78-79 21.748459723309228 28.46200650829425 27.993338707874095 21.79619506052243 80-81 21.603530135035008 28.189958471768616 28.1796023043414 22.026909088854975 82-83 21.376557023320014 28.26242564572966 28.332498903707766 22.028518427242563 84-85 21.457680261850726 28.156837418651193 28.18717516080298 22.198307158695105 86-87 21.67020602326867 28.299962649059555 28.110289536864812 21.919541790806964 88-89 21.357762019191163 28.22541735018817 28.215579347726315 22.201241282894348 90-91 21.34579054774574 28.10011397984042 28.266741539238556 22.28735393317529 92-93 21.545045710826656 28.340830203884128 28.046023379315603 22.068100705973613 94-95 21.290371473571142 28.081044671158477 28.297899982018944 22.330683873251438 96-97 21.341012066726748 28.023830779155766 28.335260548235958 22.299896605881532 98-99 21.34811997106942 28.1458890305501 28.19962196304784 22.30636903533264 100 21.463518660705585 28.078643343942705 28.142902134097973 22.314935861253737 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 659.0 1 526.0 2 370.5 3 317.0 4 301.5 5 295.0 6 277.5 7 262.5 8 248.5 9 250.5 10 238.0 11 238.0 12 238.5 13 250.0 14 280.0 15 338.0 16 438.5 17 569.0 18 737.0 19 1023.5 20 1512.0 21 2225.0 22 3345.0 23 5089.0 24 7379.0 25 10067.5 26 13646.5 27 18233.5 28 23967.5 29 30461.5 30 36928.5 31 43364.0 32 49336.5 33 55876.0 34 62178.0 35 66854.0 36 70934.0 37 74600.5 38 76939.5 39 77542.0 40 77698.5 41 77846.0 42 77225.5 43 76108.5 44 74122.0 45 72331.0 46 71241.5 47 69440.5 48 67925.0 49 66525.5 50 64783.0 51 62559.5 52 58483.5 53 54466.0 54 51980.0 55 49594.5 56 45819.5 57 41676.0 58 37946.0 59 33985.0 60 29953.0 61 25585.0 62 21620.5 63 18021.5 64 14673.0 65 11758.5 66 9030.5 67 6686.0 68 5054.0 69 3771.5 70 2639.0 71 1889.5 72 1431.0 73 1050.0 74 760.0 75 552.0 76 358.0 77 243.5 78 167.0 79 99.0 80 63.5 81 42.0 82 24.5 83 21.5 84 21.5 85 17.5 86 10.0 87 6.5 88 5.0 89 4.0 90 3.5 91 3.5 92 2.0 93 1.0 94 1.0 95 1.0 96 1.5 97 2.0 98 2.0 99 1.5 100 8.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.06257769279340233 2 0.044274893923753084 3 0.05975463898317366 4 0.07763397978128861 5 0.09151399434824628 6 0.09014951833996908 7 0.09137284165773484 8 0.10426478739111245 9 0.09923034142953799 10-11 0.10452356732371676 12-13 0.10866404624538548 14-15 0.10953448783687265 16-17 0.07504618045524565 18-19 0.1005007156441409 20-21 0.09318430118596491 22-23 0.059284130014802205 24-25 0.021878667029272245 26-27 0.18385137939114257 28-29 0.0665064426793039 30-31 0.07723404715817288 32-33 0.0707645488430655 34-35 0.02161988709666795 36-37 0.05429673495006488 38-39 0.0785749977180315 40-41 0.08363296912802455 42-43 0.07744577619394004 44-45 0.07554021487203567 46-47 0.08158625511560876 48-49 0.07819859054333435 50-51 0.07368170444696846 52-53 0.07763397978128861 54-55 0.068811936624324 56-57 0.07132915960511123 58-59 0.06876488572748685 60-61 0.06514196667102672 62-63 0.07165851588297124 64-65 0.06740040971920966 66-67 0.06947064918004402 68-69 0.06900014021167257 70-71 0.061683725753496586 72-73 0.06076623326517227 74-75 0.06271884548391377 76-77 0.057943179454943594 78-79 0.05389680232694915 80-81 0.047991914773887506 82-83 0.05422615860480916 84-85 0.04429841937217165 86-87 0.042980994260731605 88-89 0.04436899571742737 90-91 0.0404402458315258 92-93 0.043098621502824466 94-95 0.04234580715343015 96-97 0.04528648820575169 98-99 0.04528648820575169 100 0.05340276791015913 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2125358.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 73.22296324406943 #Duplication Level Percentage of deduplicated Percentage of total 1 82.9062403208022 60.70640587714086 2 11.264018347480539 16.495696028761824 3 2.9194083464531397 6.413031901403032 4 1.118350714185159 3.2755581295503475 5 0.5400610766617747 1.977243618297884 6 0.3267347791410595 1.4354693234162965 7 0.20335278728735562 1.0423065568384775 8 0.12976243961597558 0.7601272277169088 9 0.09405060948743357 0.6197997889422608 >10 0.47681033394845906 5.986198500086153 >50 0.017024117210596924 0.8250177707856031 >100 0.004121870511479857 0.4135534841809064 >500 0.0 0.0 >1k 6.425721495404246E-5 0.04959179287938409 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 4.705089683714461E-5 0.0 10-11 0.0 0.0 0.0 4.705089683714461E-5 0.0 12-13 0.0 2.3525448418572306E-5 0.0 4.705089683714461E-5 0.0 14-15 0.0 7.057634525571692E-5 0.0 4.705089683714461E-5 0.0 16-17 0.0 9.410179367428923E-5 0.0 4.705089683714461E-5 0.0 18-19 0.0 9.410179367428923E-5 0.0 4.705089683714461E-5 0.0 20-21 0.0 9.410179367428923E-5 0.0 4.705089683714461E-5 0.0 22-23 0.0 9.410179367428923E-5 0.0 7.057634525571692E-5 0.0 24-25 0.0 9.410179367428923E-5 0.0 9.410179367428923E-5 0.0 26-27 0.0 9.410179367428923E-5 0.0 2.1172903576715076E-4 0.0 28-29 0.0 9.410179367428923E-5 0.0 5.41085313627163E-4 0.0 30-31 0.0 1.1762724209286152E-4 0.0 0.002846579258647249 0.0 32-33 0.0 1.4115269051143383E-4 0.0 0.00846916143068603 0.0 34-35 0.0 1.4115269051143383E-4 0.0 0.016373712099326324 0.0 36-37 0.0 1.4115269051143383E-4 0.0 0.02928918328112252 0.0 38-39 0.0 1.4115269051143383E-4 0.0 0.05210886824713766 0.0 40-41 0.0 1.4115269051143383E-4 0.0 0.08182150959979448 0.0 42-43 0.0 1.4115269051143383E-4 0.0 0.11061665846412698 0.0 44-45 0.0 1.4115269051143383E-4 0.0 0.1433170317659425 0.0 46-47 0.0 1.4115269051143383E-4 0.0 0.18540405898676834 0.0 48-49 0.0 1.4115269051143383E-4 0.0 0.23558384046358308 0.0 50-51 0.0 1.4115269051143383E-4 0.0 0.29119800052508804 0.0 52-53 0.0 1.4115269051143383E-4 0.0 0.34704741507077863 0.0 54-55 0.0 1.4115269051143383E-4 0.0 0.40722551212548663 0.0 56-57 0.0 1.4115269051143383E-4 0.0 0.4794015878736665 0.0 58-59 0.0 1.4115269051143383E-4 0.0 0.563857947696341 0.0 60-61 0.0 1.4115269051143383E-4 0.0 0.6577950632317002 0.0 62-63 0.0 1.4115269051143383E-4 0.0 0.7534730619500338 0.0 64-65 0.0 1.4115269051143383E-4 0.0 0.8549618464277547 0.0 66-67 0.0 1.4115269051143383E-4 0.0 0.9733889537668476 0.0 68-69 0.0 1.4115269051143383E-4 0.0 1.102920072759507 0.0 70-71 0.0 1.4115269051143383E-4 0.0 1.2472251733590294 0.0 72-73 0.0 1.4115269051143383E-4 0.0 1.4121620922216398 0.0 74-75 0.0 1.4115269051143383E-4 0.0 1.5718998869837457 0.0 76-77 0.0 1.4115269051143383E-4 0.0 1.7450707127928564 0.0 78-79 0.0 1.4115269051143383E-4 0.0 1.9428726830962124 0.0 80-81 0.0 1.4115269051143383E-4 0.0 2.1660821376916264 0.0 82-83 0.0 1.4115269051143383E-4 0.0 2.406206389699994 0.0 84-85 0.0 1.4115269051143383E-4 0.0 2.63729686951563 0.0 86-87 0.0 1.4115269051143383E-4 0.0 2.8859843847483577 0.0 88 0.0 1.4115269051143383E-4 0.0 3.090632260541518 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1535 0.0 42.261463 1 TCAACGC 2660 0.0 34.460915 4 GTATCAA 2815 0.0 33.732365 1 ATCAACG 2755 0.0 33.27261 3 CAACGCA 2855 0.0 32.107193 5 AACGCAG 2935 0.0 31.06675 6 GTACATG 5915 0.0 28.610247 1 TACATGG 5885 0.0 26.998775 2 TATCAAC 3455 0.0 26.395355 2 ACATGGG 6040 0.0 25.371988 3 ACGCAGA 3570 0.0 25.27816 7 CGCAGAG 3580 0.0 25.102278 8 TATACCG 135 1.4562231E-5 24.374634 5 CATGGGG 4125 0.0 21.99415 4 GCAGAGT 4435 0.0 19.9475 9 GAGTACA 4250 0.0 19.245718 1 CTAGTAC 425 0.0 18.803288 3 TGTACGG 135 0.009983476 17.410454 5 CTGTCGC 520 0.0 17.193905 9 AGAGTAC 3810 0.0 16.433311 10-11 >>END_MODULE