FastQCFastQC Report
Wed 25 May 2016
SRR1033081_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033081_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2125358
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43320.20382448509851048No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35770.16830105798646627No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA32750.055.2657971
GTATCAA60800.042.7154051
TCAACGC72800.034.926424
ATCAACG73300.034.8829963
CAACGCA74250.034.1794475
TATCAAC77000.033.3510172
AACGCAG78000.032.656716
ACGCAGA89200.028.3982547
CGCAGAG91150.027.5844868
GTGGTAT15550.026.3709261
TGGTATC15200.026.0398832
GCAGAGT100450.024.7966869
GTACATG56550.024.5881521
TACATGG57250.022.963182
ACACCGT4000.021.1485336
AGAGTAC93600.021.1384910-11
ACATGGG60100.021.0376223
CAGAGTA98050.021.01792110-11
GAGTACT72100.020.85846912-13
TATAACG2151.1661632E-619.7245622
Produced by FastQC (version 0.11.5)