##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1033081_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2125358 Sequences flagged as poor quality 0 Sequence length 100 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.62814311753596 34.0 31.0 34.0 31.0 34.0 2 32.62490648634254 34.0 31.0 34.0 31.0 34.0 3 32.72585418550663 34.0 31.0 34.0 31.0 34.0 4 36.25102923836831 37.0 37.0 37.0 35.0 37.0 5 36.231330909898475 37.0 37.0 37.0 35.0 37.0 6 36.30811373895598 37.0 37.0 37.0 35.0 37.0 7 36.25520265291777 37.0 37.0 37.0 35.0 37.0 8 36.26263057800145 37.0 37.0 37.0 35.0 37.0 9 38.038024652787904 39.0 38.0 39.0 35.0 39.0 10-11 37.999496555403844 39.0 38.0 39.0 35.0 39.0 12-13 37.9705364931461 39.0 38.0 39.0 35.0 39.0 14-15 39.439870130114556 41.0 39.0 41.0 36.0 41.0 16-17 39.44419057871662 41.0 39.0 41.0 36.0 41.0 18-19 39.46541029793569 41.0 39.0 41.0 36.0 41.0 20-21 39.4637202297213 41.0 39.0 41.0 36.0 41.0 22-23 39.390393053782 41.0 39.0 41.0 36.0 41.0 24-25 39.332449874327054 41.0 39.0 41.0 36.0 41.0 26-27 39.220506145317636 41.0 39.0 41.0 36.0 41.0 28-29 39.164348312143176 40.5 39.0 41.0 36.0 41.0 30-31 39.150601451614264 41.0 39.0 41.0 36.0 41.0 32-33 39.10096369646902 41.0 39.0 41.0 35.0 41.0 34-35 39.013570890174734 41.0 39.0 41.0 35.0 41.0 36-37 38.918299411205076 41.0 39.0 41.0 35.0 41.0 38-39 38.81886604515569 41.0 39.0 41.0 35.0 41.0 40-41 38.71009848693726 41.0 38.5 41.0 35.0 41.0 42-43 38.57909655690947 40.5 38.0 41.0 35.0 41.0 44-45 38.401564112963555 40.0 38.0 41.0 34.5 41.0 46-47 38.18355637026797 40.0 38.0 41.0 34.0 41.0 48-49 37.95975642691725 40.0 38.0 41.0 33.5 41.0 50-51 37.81920175330462 40.0 38.0 41.0 33.0 41.0 52-53 37.68281084880759 40.0 38.0 41.0 33.0 41.0 54-55 37.53127002603797 40.0 37.0 41.0 33.0 41.0 56-57 37.38478011704381 40.0 37.0 41.0 33.0 41.0 58-59 37.2055620747187 40.0 36.5 41.0 33.0 41.0 60-61 37.01727473677376 40.0 36.0 41.0 32.0 41.0 62-63 36.78305890113572 39.0 35.5 41.0 32.0 41.0 64-65 36.51659014622478 39.0 35.0 41.0 31.5 41.0 66-67 36.2420575733594 38.5 35.0 41.0 31.0 41.0 68-69 35.93719034628519 38.0 35.0 40.0 31.0 41.0 70-71 35.61222391710008 37.0 35.0 40.0 31.0 41.0 72-73 35.270827785248414 37.0 35.0 39.0 30.5 41.0 74-75 34.92893032609095 36.0 35.0 39.0 30.0 41.0 76-77 34.58079321224942 36.0 34.5 39.0 30.0 40.5 78-79 34.21877984791268 35.0 34.0 37.5 30.0 39.5 80-81 33.88524074532385 35.0 34.0 37.0 29.5 39.0 82-83 33.58572273471104 35.0 34.0 37.0 29.0 39.0 84-85 33.35998664695548 35.0 34.0 36.0 29.0 37.5 86-87 33.142501639723754 35.0 34.0 36.0 29.0 37.0 88-89 32.92467880705274 35.0 34.0 35.5 29.0 37.0 90-91 32.730427062170236 35.0 34.0 35.0 29.0 36.0 92-93 32.54846289425123 35.0 34.0 35.0 29.0 36.0 94-95 32.383104869861924 35.0 34.0 35.0 29.0 36.0 96-97 32.22945663742297 35.0 34.0 35.0 29.0 36.0 98-99 32.09158104187624 35.0 34.0 35.0 29.0 35.5 100 31.978420106165643 35.0 34.0 35.0 28.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.19142214102724608 1101 2 0.16833457377568095 1101 3 0.1709543666023876 1101 4 0.15647283782347188 1101 5 0.08021391831358216 1101 6 0.14965115627143177 1101 7 0.19839084041487354 1101 8 0.1566333582900512 1101 9 0.18847221949956605 1101 10-11 0.25201277845452097 1101 12-13 0.23528044463235176 1101 14-15 0.36936948382732027 1101 16-17 0.35097808185069823 1101 18-19 0.3347857581991889 1101 20-21 0.3110417826646099 1101 22-23 0.329340187367805 1101 24-25 0.36478316412615186 1101 26-27 0.4503309458713787 1101 28-29 0.36437242626409017 1101 30-31 0.44010430234200015 1101 32-33 0.40566302259141906 1101 34-35 0.4215505907467829 1101 36-37 0.4050117547408263 1101 38-39 0.46277457271148137 1101 40-41 0.43571954489996045 1101 42-43 0.4243489845012576 1101 44-45 0.38044788297361265 1101 46-47 0.46996017495987275 1101 48-49 0.5238522810864055 1101 50-51 0.5218887952289393 1101 52-53 0.4191502188416294 1101 54-55 0.5529825062081812 1101 56-57 0.4997258372332496 1101 58-59 0.4386315673018757 1101 60-61 0.4356225108433307 1101 62-63 0.39044765323010466 1101 64-65 0.43580509916315435 1101 66-67 0.39648390664647337 1101 68-69 0.4669724792245873 1101 70-71 0.3951352475206136 1101 72-73 0.3975149097828137 1101 74-75 0.3778591801001312 1101 76-77 0.4231104663059071 1101 78-79 0.46371204980255953 1101 80-81 0.47812068141255537 1101 82-83 0.5240174243414515 1101 84-85 0.48031839362747775 1101 86-87 0.5457000802236607 1101 88-89 0.5157741800920306 1101 90-91 0.5230223265777667 1101 92-93 0.5543160839276737 1101 94-95 0.5589042076172319 1101 96-97 0.6219724979775805 1101 98-99 0.6527681607451399 1101 100 0.6553270588017313 1102 1 0.1913419677971362 1102 2 0.16321409135657916 1102 3 0.1946682796177015 1102 4 0.08447203329443198 1102 5 0.1736970699325795 1102 6 0.10628489675581676 1102 7 0.1812421593926885 1102 8 0.15600315941149745 1102 9 0.22496921801425884 1102 10-11 0.24170824603342567 1102 12-13 0.21678519128378326 1102 14-15 0.3287567319487792 1102 16-17 0.266719745990585 1102 18-19 0.2684643180828914 1102 20-21 0.2863108465885489 1102 22-23 0.2657092116945563 1102 24-25 0.3254205552892415 1102 26-27 0.36293187033397345 1102 28-29 0.32569729122230484 1102 30-31 0.29379130372102935 1102 32-33 0.33517595390416943 1102 34-35 0.3299479558442542 1102 36-37 0.31620504470759414 1102 38-39 0.2698547662179607 1102 40-41 0.2665757623689231 1102 42-43 0.24021851795132676 1102 44-45 0.20725948916737025 1102 46-47 0.14089130778663872 1102 48-49 0.1417478316818901 1102 50-51 0.1259938510600378 1102 52-53 0.15099255538787304 1102 54-55 0.10564484010218678 1102 56-57 0.21295283538204046 1102 58-59 0.17587703186263326 1102 60-61 0.20602735535737082 1102 62-63 0.13306338410348673 1102 64-65 0.21512909899627886 1102 66-67 0.16841198426720894 1102 68-69 0.1382561761613843 1102 70-71 0.2502599483567707 1102 72-73 0.21054283826735798 1102 74-75 0.23466128436774625 1102 76-77 0.13410653944907125 1102 78-79 0.14928312908106278 1102 80-81 0.13618564223837382 1102 82-83 0.144042809423496 1102 84-85 0.14247533552215685 1102 86-87 0.1800217039926011 1102 88-89 0.2252514483010799 1102 90-91 0.13902955288931196 1102 92-93 0.23987673369440898 1102 94-95 0.22177758122518298 1102 96-97 0.20015479838527028 1102 98-99 0.2003626259023008 1102 100 0.1000731368974499 1103 1 0.18088194404228375 1103 2 0.15685918398745713 1103 3 0.19938348048258092 1103 4 0.12208131781075338 1103 5 0.12256697618683177 1103 6 0.16826691692548934 1103 7 0.15981342957297784 1103 8 0.1568958327927703 1103 9 0.20599029774233202 1103 10-11 0.21641809932677347 1103 12-13 0.2123691268178618 1103 14-15 0.2814583030249622 1103 16-17 0.29802549280807966 1103 18-19 0.2548204890790373 1103 20-21 0.32413714288991713 1103 22-23 0.286310900965546 1103 24-25 0.2741379682814511 1103 26-27 0.28589485954511673 1103 28-29 0.3343470076806696 1103 30-31 0.33190252897458095 1103 32-33 0.298308799548785 1103 34-35 0.34279187773694275 1103 36-37 0.2704367856591503 1103 38-39 0.2402800435723833 1103 40-41 0.26392415136145786 1103 42-43 0.25982078298606126 1103 44-45 0.2598800276530042 1103 46-47 0.2531722582079965 1103 48-49 0.1695949287996541 1103 50-51 0.2237935131345239 1103 52-53 0.17657214400038868 1103 54-55 0.20280137841933055 1103 56-57 0.1840298166773522 1103 58-59 0.14443428068583586 1103 60-61 0.23018491783643213 1103 62-63 0.18718640187215385 1103 64-65 0.18447817618639561 1103 66-67 0.1719978324021909 1103 68-69 0.21648065092321644 1103 70-71 0.24498346977106422 1103 72-73 0.17855574687492037 1103 74-75 0.1437162974959847 1103 76-77 0.13873638420234613 1103 78-79 0.14189815396303374 1103 80-81 0.11264390293265336 1103 82-83 0.20560256198882598 1103 84-85 0.20650545664649655 1103 86-87 0.11843665660045843 1103 88-89 0.1110041872460954 1103 90-91 0.11364719706731563 1103 92-93 0.19822022117352844 1103 94-95 0.15056618076873463 1103 96-97 0.1751256229754503 1103 98-99 0.1523598419113199 1103 100 0.1233181912852288 1104 1 0.11695742155609423 1104 2 0.09898228714781965 1104 3 0.13328547856786344 1104 4 0.08310170520000781 1104 5 0.14901626108546395 1104 6 0.14124757198609927 1104 7 0.11862587812830583 1104 8 0.1744705523582155 1104 9 0.1700221834740958 1104 10-11 0.2244696556710153 1104 12-13 0.2155067296715174 1104 14-15 0.2795990806891737 1104 16-17 0.2718748208549613 1104 18-19 0.2566004336416725 1104 20-21 0.26838399188911666 1104 22-23 0.21491085443477687 1104 24-25 0.2595503295043855 1104 26-27 0.2182293246479432 1104 28-29 0.3084061047152957 1104 30-31 0.25417574618394667 1104 32-33 0.27538436299970925 1104 34-35 0.2571744724815517 1104 36-37 0.2903145245344376 1104 38-39 0.24481568352952365 1104 40-41 0.2675046527077498 1104 42-43 0.25989371853735577 1104 44-45 0.218439950074945 1104 46-47 0.21207624616538823 1104 48-49 0.1662040598867165 1104 50-51 0.36945939199153344 1104 52-53 0.34393370677459245 1104 54-55 0.22154438810168386 1104 56-57 0.29436862133977826 1104 58-59 0.2884704199343915 1104 60-61 0.37501626600147375 1104 62-63 0.30285314855652246 1104 64-65 0.3654936441255714 1104 66-67 0.37656228718727647 1104 68-69 0.32024568978465595 1104 70-71 0.2939154554668164 1104 72-73 0.26468412380415884 1104 74-75 0.16987262459691976 1104 76-77 0.16005629147483802 1104 78-79 0.14161883194232416 1104 80-81 0.15717438666342787 1104 82-83 0.15870679947036592 1104 84-85 0.2106963968459965 1104 86-87 0.13125354703657166 1104 88-89 0.11702343144986571 1104 90-91 0.23360699372300076 1104 92-93 0.1831638393497883 1104 94-95 0.18278942444102597 1104 96-97 0.24229546038307603 1104 98-99 0.19827134445026218 1104 100 0.2254986788689024 1105 1 0.06088515575783049 1105 2 0.0899148325610497 1105 3 0.10373877838164702 1105 4 0.10349942715597393 1105 5 0.03841753929517466 1105 6 0.10619169638976445 1105 7 0.08128065658856798 1105 8 0.09030149196834003 1105 9 0.11327514495969382 1105 10-11 0.13226357929359267 1105 12-13 0.16347874549094854 1105 14-15 0.2233025995534419 1105 16-17 0.1913482469827983 1105 18-19 0.17982708678925974 1105 20-21 0.23682791111650658 1105 22-23 0.238693276033338 1105 24-25 0.24784069869610192 1105 26-27 0.25914477541394376 1105 28-29 0.21257718851187946 1105 30-31 0.17114138351249863 1105 32-33 0.19286385246117987 1105 34-35 0.14942087521280456 1105 36-37 0.18702730894055009 1105 38-39 0.08073745161836143 1105 40-41 0.07091109536433038 1105 42-43 0.11161178121962223 1105 44-45 0.0775439029488112 1105 46-47 0.05030791833380022 1105 48-49 0.002359157757624075 1105 50-51 0.023993737041713814 1105 52-53 0.04465793041632793 1105 54-55 -0.024539843949639817 1105 56-57 -0.06483923620267973 1105 58-59 -0.0034021994233128794 1105 60-61 0.0049321923389342714 1105 62-63 0.023196112954686043 1105 64-65 0.08915786150466687 1105 66-67 0.05019564710778468 1105 68-69 0.05761266446553748 1105 70-71 0.08503988019345599 1105 72-73 0.02193679119701386 1105 74-75 0.052159167342253454 1105 76-77 0.04450842626005169 1105 78-79 0.10290345619833374 1105 80-81 0.12160496274898946 1105 82-83 0.1419791099091583 1105 84-85 0.052438497356860125 1105 86-87 0.024291792461418993 1105 88-89 0.07041099707893039 1105 90-91 0.07104186088908904 1105 92-93 0.11174021576257331 1105 94-95 0.09362093927387605 1105 96-97 0.1524153396349206 1105 98-99 0.18303872762029982 1105 100 0.14664171934229842 1106 1 0.051560326562068326 1106 2 0.1056993243517752 1106 3 0.1005446293286738 1106 4 0.06524217934710208 1106 5 0.027487401208809104 1106 6 0.0636729690930764 1106 7 0.0675661270922916 1106 8 0.06614892566994257 1106 9 0.08242960908296482 1106 10-11 0.12530867851375405 1106 12-13 0.0940410976645154 1106 14-15 0.07917397564900597 1106 16-17 0.11023956435558091 1106 18-19 0.14217741741857992 1106 20-21 0.12368441516624529 1106 22-23 0.1685172802242363 1106 24-25 0.12970051217598666 1106 26-27 0.09400264244700196 1106 28-29 0.08248148951631151 1106 30-31 0.10851551537525239 1106 32-33 0.12407907207559532 1106 34-35 0.12200906917364307 1106 36-37 0.024055323031511477 1106 38-39 0.11339395943237918 1106 40-41 0.1219308233323062 1106 42-43 0.12672438810950837 1106 44-45 0.12567552573023733 1106 46-47 0.09189694671020732 1106 48-49 -0.036949932039291866 1106 50-51 0.044947644992504365 1106 52-53 -0.001843680382137336 1106 54-55 0.013305097484426653 1106 56-57 -0.0025970502221142056 1106 58-59 0.016180098732824888 1106 60-61 -0.009424813614906213 1106 62-63 0.03039431958860206 1106 64-65 9.98210516229392E-4 1106 66-67 -0.005395525340183838 1106 68-69 -0.03922502602275557 1106 70-71 -0.004083387453178489 1106 72-73 0.08208842897282409 1106 74-75 0.1021952809105997 1106 76-77 0.06728742220089856 1106 78-79 0.10272994638297206 1106 80-81 0.037831483047874315 1106 82-83 0.04453874238988931 1106 84-85 -0.02304689880232047 1106 86-87 -0.09614029207178021 1106 88-89 0.030314349170929233 1106 90-91 0.056515070189369965 1106 92-93 0.03269404529084596 1106 94-95 0.031995350320784155 1106 96-97 0.06834385437521462 1106 98-99 0.12924813612632136 1106 100 0.08590622593920472 1107 1 0.010854582320199313 1107 2 0.04538343735405448 1107 3 0.07171392019506584 1107 4 0.022246428015264996 1107 5 0.006071650393160155 1107 6 0.016255475389790774 1107 7 0.003756019862187543 1107 8 0.03952168652237731 1107 9 0.024114323290383766 1107 10-11 0.0736142402686113 1107 12-13 0.0639787529948137 1107 14-15 0.07480304535995685 1107 16-17 -0.011988923583118094 1107 18-19 0.011411266103138473 1107 20-21 0.0293051234193058 1107 22-23 0.13251224664935535 1107 24-25 0.08852037950094882 1107 26-27 0.10924190235739673 1107 28-29 0.1436136434400126 1107 30-31 0.13526458965596788 1107 32-33 0.09819755123912444 1107 34-35 0.09099424703775583 1107 36-37 0.1551669585524138 1107 38-39 0.12221073343541633 1107 40-41 0.12369525035799 1107 42-43 0.20012972372924054 1107 44-45 0.1468741272450913 1107 46-47 0.10711419279591894 1107 48-49 0.17876500676987206 1107 50-51 0.10951205782307483 1107 52-53 0.13279519610816237 1107 54-55 0.1128154132385788 1107 56-57 0.10492136358313786 1107 58-59 0.10293501616579448 1107 60-61 0.07579055939797286 1107 62-63 0.06407115398543084 1107 64-65 0.09847034974379909 1107 66-67 0.1259091106953818 1107 68-69 0.10032668141503365 1107 70-71 0.05947849492619639 1107 72-73 0.043193796246924876 1107 74-75 0.1017450887071405 1107 76-77 0.14194379778408006 1107 78-79 0.16828621485750972 1107 80-81 0.17586656131840783 1107 82-83 0.1413884691907299 1107 84-85 0.09660369435638927 1107 86-87 0.08833313913957141 1107 88-89 0.14942356355066266 1107 90-91 0.09303462947259789 1107 92-93 0.16334082853969534 1107 94-95 0.19439750266555933 1107 96-97 0.20933922274241468 1107 98-99 0.20928219492904532 1107 100 0.16766870208456908 1108 1 -0.01933325068129932 1108 2 0.05728383922157576 1108 3 0.008798823060644168 1108 4 -0.05152624617497281 1108 5 -0.007483799534568902 1108 6 -0.01617772160001607 1108 7 -0.021642654735835265 1108 8 -0.04639936949409673 1108 9 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-0.3074771330111048 2214 72-73 -0.3412413507002441 2214 74-75 -0.35536068923865116 2214 76-77 -0.40423887488510246 2214 78-79 -0.3012545951948553 2214 80-81 -0.32284282398837405 2214 82-83 -0.24241932441892544 2214 84-85 -0.2862620729316703 2214 86-87 -0.3152456580987817 2214 88-89 -0.24700441415529895 2214 90-91 -0.27941687721646247 2214 92-93 -0.37764266401974567 2214 94-95 -0.3606396887694814 2214 96-97 -0.4386079935281231 2214 98-99 -0.49519660445647773 2214 100 -0.4227558209691118 2215 1 -0.19950754094755752 2215 2 -0.17125415026939095 2215 3 -0.13075995812199182 2215 4 -0.08989020675674908 2215 5 -0.16328914109917747 2215 6 -0.17861346711950432 2215 7 -0.16023945577182985 2215 8 -0.15034572940666635 2215 9 -0.1565663606906611 2215 10-11 -0.20028178559371668 2215 12-13 -0.23185140674911509 2215 14-15 -0.31181971024332 2215 16-17 -0.33184442956442695 2215 18-19 -0.31331753848402144 2215 20-21 -0.3179914074260779 2215 22-23 -0.2373956667565551 2215 24-25 -0.27363562055529655 2215 26-27 -0.3143976238600956 2215 28-29 -0.34766053823869214 2215 30-31 -0.3541438511609769 2215 32-33 -0.31637397705004133 2215 34-35 -0.30385011447074817 2215 36-37 -0.30058469770572316 2215 38-39 -0.2539226322382717 2215 40-41 -0.29528349883452165 2215 42-43 -0.32210082810310325 2215 44-45 -0.3602511850921175 2215 46-47 -0.32057110874281136 2215 48-49 -0.3090308917940163 2215 50-51 -0.3215873333959465 2215 52-53 -0.2993658893710389 2215 54-55 -0.2734390245894005 2215 56-57 -0.2716520210721072 2215 58-59 -0.3269365961706896 2215 60-61 -0.24729944559688022 2215 62-63 -0.25735363518322885 2215 64-65 -0.27294801610904784 2215 66-67 -0.3023263635340143 2215 68-69 -0.371061677185061 2215 70-71 -0.2473402222585861 2215 72-73 -0.2388174555592144 2215 74-75 -0.3390599843538098 2215 76-77 -0.2966308314928767 2215 78-79 -0.3015089071882713 2215 80-81 -0.28972540026531846 2215 82-83 -0.27086816281332915 2215 84-85 -0.28907490929658053 2215 86-87 -0.21906701325627864 2215 88-89 -0.2275204031284943 2215 90-91 -0.17854955882645385 2215 92-93 -0.16963568039729182 2215 94-95 -0.2258933984632847 2215 96-97 -0.2425761210535491 2215 98-99 -0.177808783454374 2215 100 -0.26319270468753686 2216 1 -0.4551636745049521 2216 2 -0.41530288211372834 2216 3 -0.4023403763147684 2216 4 -0.3358889658780555 2216 5 -0.3939899088393872 2216 6 -0.36238614510818223 2216 7 -0.3534166643411467 2216 8 -0.3803828289326532 2216 9 -0.4712145906448342 2216 10-11 -0.480009982576874 2216 12-13 -0.5354553735682543 2216 14-15 -0.6573890025559663 2216 16-17 -0.6488671702762474 2216 18-19 -0.6374975089728139 2216 20-21 -0.6180637032070138 2216 22-23 -0.6898540047461239 2216 24-25 -0.6882971418857835 2216 26-27 -0.7551692696844796 2216 28-29 -0.7225244067016448 2216 30-31 -0.7291490014754842 2216 32-33 -0.7052494403062894 2216 34-35 -0.7527282990443638 2216 36-37 -0.7834024604781504 2216 38-39 -0.7505446263214708 2216 40-41 -0.6309017455901653 2216 42-43 -0.6717365986115382 2216 44-45 -0.8021141347164544 2216 46-47 -0.871552955694753 2216 48-49 -0.9344228316662679 2216 50-51 -0.9517579395810216 2216 52-53 -0.9443034322856363 2216 54-55 -0.9339768607698531 2216 56-57 -0.990530105829059 2216 58-59 -0.8961400119418315 2216 60-61 -0.975872617475396 2216 62-63 -0.9256339044540525 2216 64-65 -1.041829369298739 2216 66-67 -0.9329473830163479 2216 68-69 -0.9139649040325324 2216 70-71 -1.0258171085851941 2216 72-73 -1.077220962381162 2216 74-75 -1.1764920424179834 2216 76-77 -1.0137975858806314 2216 78-79 -0.9865363106139711 2216 80-81 -1.0273370082374171 2216 82-83 -1.0591913997790599 2216 84-85 -1.1155168153625041 2216 86-87 -1.1477216670504404 2216 88-89 -1.2157069137849739 2216 90-91 -1.1641765226535696 2216 92-93 -1.268934119377306 2216 94-95 -1.2444860901275945 2216 96-97 -1.3955159956252885 2216 98-99 -1.4186292343720055 2216 100 -1.5056200537319135 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 11.0 3 4.0 4 6.0 5 22.0 6 43.0 7 124.0 8 259.0 9 590.0 10 1099.0 11 1651.0 12 2525.0 13 3949.0 14 4805.0 15 6312.0 16 7749.0 17 11050.0 18 12741.0 19 6452.0 20 4544.0 21 4164.0 22 4398.0 23 4795.0 24 5225.0 25 6592.0 26 8269.0 27 10689.0 28 13951.0 29 18024.0 30 23188.0 31 29934.0 32 38251.0 33 49349.0 34 69234.0 35 104976.0 36 187616.0 37 413404.0 38 727738.0 39 340443.0 40 1182.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.60048821404599 20.23645337819455 13.845424617247154 23.317633790512303 2 15.738338333711447 20.448567168811223 40.208605618503775 23.604488878973555 3 18.11179117708504 25.67793292567017 30.491501371110875 25.71877452613392 4 12.287454136016178 17.228342648070445 39.33508868840534 31.149114527508036 5 12.187010816556121 37.82082317619708 36.02225136247955 13.969914644767245 6 30.056159950464817 38.99738302911791 17.598823351171898 13.34763366924537 7 26.439780968665044 32.98023203620284 22.824625310183038 17.75536168494908 8 24.82186480168932 36.30504499960477 20.429510029397484 18.443580169308422 9 24.843108784496543 16.403542367921077 21.727116090559804 37.02623275702258 10-11 23.55394901521639 26.840811352536537 29.075583200805514 20.529656431441555 12-13 24.684453160361688 24.161200136635806 29.432523838336884 21.721822864665626 14-15 21.779234268197865 26.10562247909464 26.981453664745025 25.133689587962465 16-17 20.687476224543353 29.6575134442341 27.402947321469114 22.252063009753435 18-19 20.458391353311733 27.934611102053758 30.486384449022523 21.120613095611983 20-21 21.71210685446875 27.323843794786573 30.156448937073186 20.807600413671484 22-23 21.291402779440897 27.1529163003554 30.268489200983083 21.28719171922062 24-25 21.075669514463065 27.255352410393225 30.263594669527404 21.40538340561631 26-27 20.890454223262154 27.15663535501271 30.39202654993628 21.560883871788857 28-29 21.017426281657684 27.242509832484295 30.34797043126483 21.39209345459319 30-31 21.302587403654307 27.771131827655104 29.86614703570628 21.06013373298431 32-33 20.868107867004053 27.350662805983745 30.428850104311838 21.352379222700364 34-35 20.999450445524943 27.12634765531266 30.42774440823617 21.446457490926235 36-37 21.4839100048086 27.640331652361628 29.675918127675434 21.199840215154342 38-39 21.321043325406823 27.77339158861707 29.77576013076385 21.12980495521225 40-41 21.053042655687484 27.46319380451882 30.058053817349617 21.42570972244408 42-43 21.056805488769424 27.45918569953862 29.756022279540673 21.727986532151288 44-45 21.3550846492685 27.71638942709887 29.540835002855992 21.38769092077664 46-47 21.434388935887508 28.074399701132702 29.085782254095545 21.405429108884245 48-49 21.410369453052144 27.82415950630435 29.048000383935317 21.717470656708187 50-51 21.48520390447162 27.845944071539947 28.795337067919853 21.873514956068576 52-53 21.520303873512134 27.816160853842035 28.78651502476289 21.877020247882946 54-55 21.64990556885005 28.304549163011593 28.54716711255233 21.498378155586025 56-57 21.399218390501744 27.92858897183439 28.71226870955387 21.959923928109994 58-59 21.21388490786023 27.56253299444141 29.13989078545826 22.083691312240102 60-61 21.585351738389484 28.01316295889916 28.639269243111045 21.762216059600313 62-63 21.389620007546963 27.914191397402227 28.724125535556833 21.972063059493976 64-65 21.411992708993026 27.67258504214349 28.9138347516042 22.001587497259283 66-67 21.297946345497166 27.774903751486512 28.699783448196364 22.227366454819954 68-69 21.807498665514856 27.934555766422637 28.544454881933646 21.713490686128857 70-71 21.768591456121744 27.66131635235099 28.665852999824033 21.904239191703233 72-73 21.480593087918184 27.882241568569142 28.776658537813283 21.860506805699387 74-75 21.538163307459985 27.894048659210963 28.584192837613326 21.983595195715726 76-77 21.636237283318856 27.903369691129683 28.370796825758294 22.089596199793167 78-79 21.768873761502768 28.007281596794513 28.503997914704254 21.719846726998462 80-81 21.544511559934847 27.84839071817548 28.599440658938402 22.007657062951278 82-83 21.45002163168544 27.88513633843201 28.678321663515426 21.986520366367117 84-85 21.454809025114827 27.79898727649648 28.52500614014204 22.221197558246658 86-87 21.591855459100852 27.938033735620017 28.422448067377136 22.047662737901994 88-89 21.31778039910582 27.84963165217539 28.513866976959967 22.318720971758825 90-91 21.208021260037395 27.719331182109645 28.61195726536746 22.46069029248549 92-93 21.41296551114093 27.960581337984515 28.362140901319954 22.2643122495546 94-95 21.124198923986206 27.682171055092482 28.637169174220933 22.556460846700382 96-97 21.113445106322512 27.681117858498094 28.622610069543857 22.582826965635533 98-99 21.114487542895805 27.69769828877878 28.5202908598499 22.667523308475506 100 21.253689966584453 27.673596636425486 28.38241839727707 22.69029499971299 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 28.0 1 26.5 2 100.0 3 186.0 4 133.0 5 97.5 6 254.5 7 568.0 8 980.5 9 1179.5 10 968.0 11 754.0 12 791.0 13 935.5 14 1107.0 15 1252.5 16 1422.5 17 1732.5 18 2035.5 19 2376.5 20 2851.5 21 3484.5 22 4527.5 23 6141.5 24 8228.0 25 10826.5 26 14291.5 27 18603.0 28 24263.0 29 30570.5 30 36835.0 31 43059.0 32 48909.0 33 55167.5 34 61420.5 35 66211.5 36 70224.5 37 73982.0 38 76201.0 39 76827.5 40 77058.0 41 77471.5 42 76929.5 43 75657.0 44 73790.0 45 71938.5 46 71113.0 47 69560.0 48 67492.5 49 65665.0 50 64416.0 51 62472.5 52 57728.0 53 53884.5 54 51506.0 55 48735.0 56 45451.5 57 41601.5 58 37836.5 59 34083.0 60 30132.0 61 25429.5 62 21286.0 63 17613.0 64 14146.5 65 11223.5 66 8657.5 67 6431.5 68 4666.5 69 3551.0 70 2546.5 71 1760.0 72 1270.5 73 894.0 74 642.5 75 453.0 76 287.5 77 180.0 78 111.5 79 63.0 80 41.0 81 22.0 82 8.5 83 4.5 84 0.5 85 1.0 86 2.0 87 2.0 88 1.0 89 0.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.041404789216687256 2 0.24395890010059482 3 0.0034817663659487013 4 0.0024466466355315196 5 2.3525448418572307E-4 6 0.0 7 0.0 8 2.823053810228677E-4 9 0.0 10-11 8.46916143068603E-4 12-13 0.0 14-15 2.587799326042954E-4 16-17 2.1172903576715076E-4 18-19 5.881362104643076E-4 20-21 0.0 22-23 1.1762724209286154E-4 24-25 0.0014350523535329107 26-27 2.1172903576715076E-4 28-29 2.3525448418572307E-4 30-31 1.6467813893000614E-4 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 1.1762724209286154E-4 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 2.3525448418572306E-5 68-69 3.0583082944143995E-4 70-71 0.0 72-73 3.293562778600123E-4 74-75 2.3525448418572307E-4 76-77 0.0 78-79 0.0 80-81 0.0 82-83 1.6467813893000614E-4 84-85 0.0 86-87 3.764071746971569E-4 88-89 0.002681901119717243 90-91 0.0026348502228800983 92-93 9.174924883243199E-4 94-95 6.351871073014523E-4 96-97 3.9993262311572924E-4 98-99 2.1172903576715076E-4 100 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2125358.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 70.87214516033826 #Duplication Level Percentage of deduplicated Percentage of total 1 81.5979273598329 57.830201526288135 2 12.117741106553662 17.176206134381385 3 3.099927580022648 6.590955523137036 4 1.214338190768731 3.442510101196162 5 0.5995780771190398 2.124669225826853 6 0.3428608891142857 1.457957202186618 7 0.22176094606223634 1.100167177215173 8 0.1489265122820944 0.8443793117343594 9 0.11602602168961176 0.7400711746406449 >10 0.511501769061174 6.272518899075117 >50 0.022892512343369517 1.1068267392863704 >100 0.006054038465044602 0.7221303615122907 >500 1.9928429362263064E-4 0.07617385104739456 >1k 2.6571239149612315E-4 0.5152327724725396 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4332 0.20382448509851048 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3577 0.16830105798646627 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 4.705089683714461E-5 0.0 16-17 0.0 0.0 0.0 4.705089683714461E-5 0.0 18-19 0.0 0.0 0.0 4.705089683714461E-5 0.0 20-21 0.0 0.0 0.0 4.705089683714461E-5 0.0 22-23 0.0 0.0 0.0 7.057634525571692E-5 0.0 24-25 0.0 0.0 0.0 9.410179367428923E-5 0.0 26-27 0.0 0.0 0.0 2.1172903576715076E-4 0.0 28-29 0.0 0.0 0.0 5.175598652085908E-4 0.0 30-31 0.0 2.3525448418572306E-5 0.0 0.002799528361810105 0.0 32-33 0.0 4.705089683714461E-5 0.0 0.008328008740174597 0.0 34-35 0.0 4.705089683714461E-5 0.0 0.016303135754070608 0.0 36-37 0.0 1.1762724209286154E-4 0.0 0.029265657832703948 0.0 38-39 0.0 1.8820358734857845E-4 0.0 0.05225002093764909 0.0 40-41 0.0 1.8820358734857845E-4 0.0 0.08203323863556164 0.0 42-43 0.0 2.3525448418572307E-4 0.0 0.11096954019040557 0.0 44-45 0.0 2.3525448418572307E-4 0.0 0.14385811707956964 0.0 46-47 0.0 2.3525448418572307E-4 0.0 0.1861098224393255 0.0 48-49 0.0 2.3525448418572307E-4 0.0 0.23638370570981454 0.0 50-51 0.0 2.3525448418572307E-4 0.0 0.29220959480708664 0.0 52-53 0.0 2.3525448418572307E-4 0.0 0.3488824000474273 0.0 54-55 0.0 2.3525448418572307E-4 0.0 0.40981331145152955 0.0 56-57 0.0 2.3525448418572307E-4 0.0 0.4827657269975223 0.0 58-59 0.0 2.3525448418572307E-4 0.0 0.5678337484790797 0.0 60-61 0.0 2.3525448418572307E-4 0.0 0.6619590676017876 0.0 62-63 0.0 2.3525448418572307E-4 0.0 0.7587192369473754 0.0 64-65 0.0 2.3525448418572307E-4 0.0 0.8611960902586764 0.0 66-67 0.0 2.3525448418572307E-4 0.0 0.9807288936734423 0.0 68-69 0.0 2.3525448418572307E-4 0.0 1.1112245560512628 0.0 70-71 0.0 2.3525448418572307E-4 0.0 1.256917658107481 0.0 72-73 0.0 2.3525448418572307E-4 0.0 1.4232190529783688 0.0 74-75 0.0 2.3525448418572307E-4 0.0 1.5849094599592162 0.0 76-77 0.0 2.3525448418572307E-4 0.0 1.75984469439972 0.0 78-79 0.0 2.3525448418572307E-4 0.0 1.9593169715407943 0.0 80-81 0.0 2.3525448418572307E-4 0.0 2.18447903835495 0.0 82-83 0.0 2.3525448418572307E-4 0.0 2.426838207963082 0.0 84-85 0.0 2.3525448418572307E-4 0.0 2.6606576397952724 0.0 86-87 0.0 2.3525448418572307E-4 0.0 2.9118153271119502 0.0 88 0.0 2.3525448418572307E-4 0.0 3.1190039513343164 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3275 0.0 55.265797 1 GTATCAA 6080 0.0 42.715405 1 TCAACGC 7280 0.0 34.92642 4 ATCAACG 7330 0.0 34.882996 3 CAACGCA 7425 0.0 34.179447 5 TATCAAC 7700 0.0 33.351017 2 AACGCAG 7800 0.0 32.65671 6 ACGCAGA 8920 0.0 28.398254 7 CGCAGAG 9115 0.0 27.584486 8 GTGGTAT 1555 0.0 26.370926 1 TGGTATC 1520 0.0 26.039883 2 GCAGAGT 10045 0.0 24.796686 9 GTACATG 5655 0.0 24.588152 1 TACATGG 5725 0.0 22.96318 2 ACACCGT 400 0.0 21.148533 6 AGAGTAC 9360 0.0 21.13849 10-11 ACATGGG 6010 0.0 21.037622 3 CAGAGTA 9805 0.0 21.017921 10-11 GAGTACT 7210 0.0 20.858469 12-13 TATAACG 215 1.1661632E-6 19.724562 2 >>END_MODULE