FastQCFastQC Report
Wed 25 May 2016
SRR1033080_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033080_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3939772
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA46050.036.3425371
TCAACGC44200.035.3070144
ATCAACG45950.033.8600543
CAACGCA46700.033.5179945
GGTATCA29350.032.9954451
AACGCAG49500.031.900836
TATCAAC56050.028.7649542
ACGCAGA59950.026.105297
GTACATG96650.025.7305261
CGCAGAG62650.024.8513628
TACATGG98150.024.3286822
ACATGGG100100.022.4460013
CATGGGG74350.019.9147454
GCAGAGT77600.018.793349
TATACTG24800.018.3853025
TAGACCG1350.00998800817.4095255
CCTACAC14600.017.0635033
GAGTACA74850.016.9576951
AGAGTAC65150.016.39763310-11
GTATAGG14200.016.2219311
Produced by FastQC (version 0.11.5)