##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1033080_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3939772
Sequences flagged as poor quality	0
Sequence length	100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.58713397628086	33.0	31.0	34.0	30.0	34.0
2	31.667384813131317	34.0	31.0	34.0	30.0	34.0
3	31.82213767700263	34.0	31.0	34.0	30.0	34.0
4	35.16793789082211	37.0	35.0	37.0	33.0	37.0
5	35.24817146779052	37.0	35.0	37.0	33.0	37.0
6	35.28918323192307	37.0	35.0	37.0	33.0	37.0
7	35.19421428448144	37.0	35.0	37.0	33.0	37.0
8	35.20582510866111	37.0	35.0	37.0	33.0	37.0
9	36.83761852208706	39.0	38.0	39.0	33.0	39.0
10-11	36.822079551811626	39.0	38.0	39.0	33.0	39.0
12-13	36.755773811276384	39.0	38.0	39.0	33.0	39.0
14-15	38.14619145473392	41.0	39.0	41.0	33.0	41.0
16-17	38.147055971766896	41.0	39.0	41.0	33.0	41.0
18-19	38.16117480910063	41.0	38.5	41.0	33.5	41.0
20-21	38.18436841015165	41.0	39.0	41.0	34.0	41.0
22-23	38.14891750080969	40.0	39.0	41.0	33.0	41.0
24-25	38.15954311569298	40.0	38.5	41.0	33.0	41.0
26-27	38.06192236505056	40.0	38.0	41.0	33.0	41.0
28-29	38.06713040754643	40.0	38.0	41.0	33.0	41.0
30-31	38.21171885073552	40.5	39.0	41.0	33.0	41.0
32-33	38.19362668702656	41.0	39.0	41.0	33.0	41.0
34-35	38.150460356589164	41.0	38.0	41.0	33.0	41.0
36-37	38.07614577696374	40.5	38.0	41.0	33.0	41.0
38-39	37.98994319975877	40.0	38.0	41.0	33.0	41.0
40-41	37.92259602839961	40.0	38.0	41.0	33.0	41.0
42-43	37.80662535801564	40.0	38.0	41.0	32.5	41.0
44-45	37.68784551491812	40.0	38.0	41.0	32.0	41.0
46-47	37.56679193618311	40.0	38.0	41.0	32.0	41.0
48-49	37.456275134703226	40.0	38.0	41.0	32.0	41.0
50-51	37.33785165740555	40.0	37.0	41.0	31.0	41.0
52-53	37.18900814564904	40.0	37.0	41.0	31.0	41.0
54-55	37.038226831400394	40.0	37.0	41.0	31.0	41.0
56-57	36.8709767976421	40.0	36.0	41.0	30.5	41.0
58-59	36.68204086429367	40.0	36.0	41.0	30.0	41.0
60-61	36.46485672267329	39.0	35.5	41.0	30.0	41.0
62-63	36.219394041076484	39.0	35.0	41.0	30.0	41.0
64-65	35.94771156808059	39.0	35.0	41.0	30.0	41.0
66-67	35.65514349561345	38.0	35.0	40.0	29.0	41.0
68-69	35.34312467828087	37.5	35.0	40.0	29.0	41.0
70-71	35.00702540654637	37.0	34.5	40.0	29.0	41.0
72-73	34.65480870466616	36.5	34.0	39.0	29.0	41.0
74-75	34.29463088219318	36.0	34.0	39.0	28.0	41.0
76-77	33.92478130714163	35.5	34.0	38.5	28.0	40.0
78-79	33.53265925033226	35.0	34.0	37.0	27.5	39.0
80-81	33.171108759593196	35.0	34.0	37.0	27.0	39.0
82-83	32.93467566143421	35.0	34.0	36.5	27.0	39.0
84-85	32.699660031088094	35.0	34.0	36.0	27.0	37.5
86-87	32.50876510112768	35.0	34.0	36.0	27.0	37.0
88-89	32.285845729143716	35.0	33.5	35.5	27.0	37.0
90-91	32.06967027533573	35.0	33.0	35.0	26.5	36.0
92-93	31.847846144396172	35.0	33.0	35.0	26.0	36.0
94-95	31.645368564475305	35.0	33.0	35.0	25.5	36.0
96-97	31.471068884189236	35.0	33.0	35.0	25.0	36.0
98-99	31.297558589684886	35.0	33.0	35.0	25.0	35.0
100	31.160107996097235	35.0	33.0	35.0	24.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.12140928619961855
1101	2	0.11780041068680092
1101	3	0.12860213168258028
1101	4	0.0988009456823491
1101	5	0.08934157083334782
1101	6	0.16686774179689934
1101	7	0.2076711906885862
1101	8	0.2267872309603689
1101	9	0.2874826596725484
1101	10-11	0.32652860487920776
1101	12-13	0.3214705259621695
1101	14-15	0.4155299203419389
1101	16-17	0.3821718081363983
1101	18-19	0.36367272633951586
1101	20-21	0.37258549670983854
1101	22-23	0.3204455369746526
1101	24-25	0.3350795943441156
1101	26-27	0.3481320376499468
1101	28-29	0.26256810719841184
1101	30-31	0.34816361721827604
1101	32-33	0.2792797244171936
1101	34-35	0.32280983658726825
1101	36-37	0.325390810792193
1101	38-39	0.3891222818111686
1101	40-41	0.3415256504132316
1101	42-43	0.3946857513488027
1101	44-45	0.32601268633619185
1101	46-47	0.29032739443462674
1101	48-49	0.3693836333381597
1101	50-51	0.3747214080100676
1101	52-53	0.29094871799437527
1101	54-55	0.2992044434540446
1101	56-57	0.36582322297781644
1101	58-59	0.43278577957116937
1101	60-61	0.37564937316730607
1101	62-63	0.3290046297040803
1101	64-65	0.24966148014269862
1101	66-67	0.28546252412616013
1101	68-69	0.3248999237208281
1101	70-71	0.2904024087927226
1101	72-73	0.25257399581469286
1101	74-75	0.25624439070377747
1101	76-77	0.24816370401242693
1101	78-79	0.2234244471951783
1101	80-81	0.19316494199077994
1101	82-83	0.18436051848218682
1101	84-85	0.23434607785168282
1101	86-87	0.2159172030585168
1101	88-89	0.2995502098862346
1101	90-91	0.3110501491938322
1101	92-93	0.31109576498891656
1101	94-95	0.3103309153481426
1101	96-97	0.3555522766471739
1101	98-99	0.37235834027702097
1101	100	0.38365152045057727
1102	1	-0.08846965543698815
1102	2	-0.0699095154952154
1102	3	-0.010059981645131444
1102	4	-0.20558013826352806
1102	5	-0.08657726851805592
1102	6	-0.048234196791725026
1102	7	-0.017378761120639297
1102	8	0.015008331588525436
1102	9	0.027918998649383298
1102	10-11	0.04821114226340484
1102	12-13	0.059307875379964514
1102	14-15	0.15666207656056486
1102	16-17	0.16182933215910822
1102	18-19	0.08685263000268151
1102	20-21	0.0986814455538223
1102	22-23	0.0932325907627174
1102	24-25	0.13029576520111164
1102	26-27	0.058738453172971106
1102	28-29	0.1156584945359782
1102	30-31	0.21411456884635527
1102	32-33	0.18519030648575097
1102	34-35	0.16041691012125625
1102	36-37	0.23513156733148577
1102	38-39	0.30103986943229444
1102	40-41	0.162656453712124
1102	42-43	0.21846669799009533
1102	44-45	0.21843795708785763
1102	46-47	0.21236546458966643
1102	48-49	0.1633886754799363
1102	50-51	0.1985533686702965
1102	52-53	0.14166960897587444
1102	54-55	0.19254461220003805
1102	56-57	0.19209533091621722
1102	58-59	0.13047963843368393
1102	60-61	0.15914664552458646
1102	62-63	0.18134664837214132
1102	64-65	0.1694482946540532
1102	66-67	0.18246570907811588
1102	68-69	0.188089898809217
1102	70-71	0.08986972459996423
1102	72-73	0.17342134286208477
1102	74-75	0.2338904669011086
1102	76-77	0.19157399257567675
1102	78-79	0.16173598938950562
1102	80-81	0.28976026636726715
1102	82-83	0.22988459039042652
1102	84-85	0.2259428110536703
1102	86-87	0.19586408139189615
1102	88-89	0.20004324290865583
1102	90-91	0.22017880738815876
1102	92-93	0.22870309289417534
1102	94-95	0.27319256980126383
1102	96-97	0.28527152064457084
1102	98-99	0.2676827447128076
1102	100	0.15428368617318
1103	1	0.07859979133534978
1103	2	0.149066817298106
1103	3	0.14133568502337113
1103	4	0.17100584200490943
1103	5	0.12972888755246714
1103	6	0.19310926796217132
1103	7	0.23940377135905067
1103	8	0.19942875229794765
1103	9	0.33930070421569525
1103	10-11	0.3133192940981502
1103	12-13	0.3166620173461041
1103	14-15	0.35906894194970107
1103	16-17	0.42235351932847465
1103	18-19	0.3995925759459169
1103	20-21	0.4041859330125561
1103	22-23	0.4320640853533462
1103	24-25	0.397413505263323
1103	26-27	0.34366583917802274
1103	28-29	0.33356477746207247
1103	30-31	0.34519160329696064
1103	32-33	0.3017201970482972
1103	34-35	0.2674004585476766
1103	36-37	0.34148302205091596
1103	38-39	0.3691553672772443
1103	40-41	0.3959512832386878
1103	42-43	0.33268264708765116
1103	44-45	0.3284658947493213
1103	46-47	0.3067162619128254
1103	48-49	0.28926840166651857
1103	50-51	0.3249866430830437
1103	52-53	0.295684091183233
1103	54-55	0.313545227526177
1103	56-57	0.34088411087825676
1103	58-59	0.33824320480913883
1103	60-61	0.2924540188064526
1103	62-63	0.3266320142687391
1103	64-65	0.2890009681439665
1103	66-67	0.29610890620949704
1103	68-69	0.2408792328067122
1103	70-71	0.20663664610233212
1103	72-73	0.23080820007106695
1103	74-75	0.20852998766715558
1103	76-77	0.21399661880859355
1103	78-79	0.27058261982029563
1103	80-81	0.29230179651320043
1103	82-83	0.23316464967033568
1103	84-85	0.1909446249082407
1103	86-87	0.22536569679101603
1103	88-89	0.22075870201682335
1103	90-91	0.2097069054672147
1103	92-93	0.2231054798972103
1103	94-95	0.19502350242627742
1103	96-97	0.21258909452302177
1103	98-99	0.13542215167443317
1103	100	0.111976586817633
1104	1	-0.09083947782752233
1104	2	-0.036368200848375665
1104	3	0.006002483043594964
1104	4	2.4228059726993934E-4
1104	5	0.004939318140330329
1104	6	0.016188251131197262
1104	7	0.11597246937717642
1104	8	0.12630408279993333
1104	9	0.16141409716946953
1104	10-11	0.1167522549441884
1104	12-13	0.1795581322965134
1104	14-15	0.2022506659509986
1104	16-17	0.1680763780777923
1104	18-19	0.17963976670642268
1104	20-21	0.1609714467656218
1104	22-23	0.235470261784684
1104	24-25	0.11027050370753244
1104	26-27	0.013959957468557604
1104	28-29	0.02749867539263562
1104	30-31	0.10254131103958741
1104	32-33	0.09822575913364062
1104	34-35	0.06453025031969162
1104	36-37	0.13508906265872866
1104	38-39	0.10124679282840532
1104	40-41	0.1992080460701473
1104	42-43	0.14809457948166482
1104	44-45	0.16391958316108912
1104	46-47	0.0980212719528879
1104	48-49	0.0676038511562993
1104	50-51	0.0068115242953652455
1104	52-53	0.01648961001455973
1104	54-55	-0.01353282158264335
1104	56-57	0.038702183283234604
1104	58-59	0.026647319564446548
1104	60-61	0.02694196578765684
1104	62-63	-0.041455016772353304
1104	64-65	-0.06104585069120816
1104	66-67	-0.05240362413491084
1104	68-69	-0.028480654750858037
1104	70-71	-0.10347073350099834
1104	72-73	-0.09317147942195447
1104	74-75	-0.06206251382922545
1104	76-77	-0.09335951075885873
1104	78-79	-0.0975273669924448
1104	80-81	-0.07310537742442591
1104	82-83	-0.03516758504620299
1104	84-85	-0.012965252952255923
1104	86-87	-0.08204218380473804
1104	88-89	-0.023877497745431242
1104	90-91	0.019767504843542838
1104	92-93	0.004354092416274824
1104	94-95	0.08786904904518522
1104	96-97	0.12052158608681296
1104	98-99	0.09065622525928063
1104	100	0.0917661452907943
1105	1	-0.06669475944767811
1105	2	-0.03831470724729513
1105	3	-0.005610714188414079
1105	4	0.059129061911399106
1105	5	-0.028897243875881884
1105	6	0.11241769796187384
1105	7	0.07283393217544187
1105	8	0.15549539058294926
1105	9	0.18090086161642915
1105	10-11	0.1827644925227716
1105	12-13	0.20285505324815745
1105	14-15	0.19974364659270805
1105	16-17	0.18821014906990996
1105	18-19	0.20544863893856302
1105	20-21	0.17317277572137613
1105	22-23	0.1730832336278496
1105	24-25	0.1488560136214474
1105	26-27	0.14532867191864085
1105	28-29	0.1370097887395545
1105	30-31	0.14820795156705913
1105	32-33	0.1329106734192962
1105	34-35	0.06936538355803634
1105	36-37	0.12773760105892507
1105	38-39	0.16406363800599877
1105	40-41	0.20065969572414843
1105	42-43	0.21468422185319014
1105	44-45	0.13124140637437165
1105	46-47	0.12319287818623792
1105	48-49	0.12058705993393204
1105	50-51	0.09608751564871909
1105	52-53	0.1472878860118385
1105	54-55	0.11591016219084338
1105	56-57	0.09337235605340055
1105	58-59	0.13444465159180652
1105	60-61	0.17233663546476663
1105	62-63	0.10308938951545343
1105	64-65	0.062077121364026766
1105	66-67	0.08658361550274662
1105	68-69	0.07523939553501435
1105	70-71	0.06824665305369138
1105	72-73	0.08670164194722219
1105	74-75	0.07799902176420659
1105	76-77	0.11914542732824884
1105	78-79	0.16102477106981183
1105	80-81	0.2645335856487847
1105	82-83	0.20429573304256365
1105	84-85	0.1730399122498767
1105	86-87	0.07499231548432306
1105	88-89	0.2093956414773075
1105	90-91	0.2269451038555843
1105	92-93	0.23667857640158374
1105	94-95	0.310442999519811
1105	96-97	0.28320128683750667
1105	98-99	0.24600610321886762
1105	100	0.32544172936601257
1106	1	-0.06089485565729191
1106	2	0.027948486827096275
1106	3	0.04760399203067678
1106	4	-0.014654336417606828
1106	5	0.022070529672504335
1106	6	0.10836035665472821
1106	7	0.10882843663703312
1106	8	0.1632178376240745
1106	9	0.12992260489506435
1106	10-11	0.13910089919261992
1106	12-13	0.1433302425224383
1106	14-15	0.15494139221580383
1106	16-17	0.14865101243105272
1106	18-19	0.184796140339067
1106	20-21	0.2376262678900929
1106	22-23	0.2267816381595935
1106	24-25	0.1643178692420051
1106	26-27	0.15362121510813154
1106	28-29	0.1707269711076549
1106	30-31	0.20204434528017146
1106	32-33	0.13866210725822725
1106	34-35	0.1406154465748557
1106	36-37	0.15322672941960747
1106	38-39	0.2058422626042784
1106	40-41	0.19836635440004358
1106	42-43	0.19231089274140345
1106	44-45	0.18100167152090307
1106	46-47	0.1273446269443923
1106	48-49	0.1663626003591716
1106	50-51	0.18973464215211067
1106	52-53	0.12036232737650465
1106	54-55	0.12288276789302444
1106	56-57	0.1538206283087007
1106	58-59	0.1682145442959353
1106	60-61	0.1307457067781428
1106	62-63	0.050001953727836224
1106	64-65	0.0656722607525353
1106	66-67	0.10124274276564904
1106	68-69	0.13699294061431289
1106	70-71	0.10246308181122998
1106	72-73	0.15199873506936967
1106	74-75	0.14387639973654132
1106	76-77	0.1294386075177414
1106	78-79	0.14253439744953056
1106	80-81	0.16083698328048968
1106	82-83	0.13821999930290474
1106	84-85	0.11809177215322819
1106	86-87	0.08472558822727194
1106	88-89	0.13336277802569896
1106	90-91	0.05432658628460274
1106	92-93	0.05529854088600317
1106	94-95	0.0909771843223659
1106	96-97	0.051244417673625975
1106	98-99	-0.04539936753685225
1106	100	-0.018716127901701185
1107	1	-0.011722591161987594
1107	2	-0.007738833055519478
1107	3	0.023681833510810435
1107	4	0.004464410059505042
1107	5	-0.005208776269256532
1107	6	-0.02005944335516574
1107	7	-0.01346561118958789
1107	8	-0.005772013868295289
1107	9	-0.03734504008559014
1107	10-11	0.029809806392847804
1107	12-13	-0.015524232041713049
1107	14-15	0.030190255565145208
1107	16-17	-0.020098161264854753
1107	18-19	0.023084964176838696
1107	20-21	0.05323554521820739
1107	22-23	-0.021056629178929143
1107	24-25	-0.04745318966366341
1107	26-27	0.019574361736104606
1107	28-29	0.009896721046203538
1107	30-31	0.0292206682317655
1107	32-33	-0.012085598617304072
1107	34-35	0.005518362818250466
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2216	34-35	-0.7757492696341544
2216	36-37	-0.7634878386476984
2216	38-39	-0.6808021310033041
2216	40-41	-0.5704157379780739
2216	42-43	-0.6088237432104577
2216	44-45	-0.47656268362076304
2216	46-47	-0.450204573255121
2216	48-49	-0.5158242293332833
2216	50-51	-0.49100063467367505
2216	52-53	-0.49323261438537713
2216	54-55	-0.5232109961497287
2216	56-57	-0.5661819387070679
2216	58-59	-0.48457274614870016
2216	60-61	-0.49295763195260633
2216	62-63	-0.5455767875619131
2216	64-65	-0.6006231310515844
2216	66-67	-0.6292907209343923
2216	68-69	-0.6617261072793568
2216	70-71	-0.5920257637469177
2216	72-73	-0.53793328301321
2216	74-75	-0.6082287966073991
2216	76-77	-0.41784194727222257
2216	78-79	-0.47095793857601365
2216	80-81	-0.4542864656503127
2216	82-83	-0.6393532795420427
2216	84-85	-0.4324411785181326
2216	86-87	-0.4154544867123633
2216	88-89	-0.5551698326237293
2216	90-91	-0.4952237459482731
2216	92-93	-0.663254345630552
2216	94-95	-0.5902072266458127
2216	96-97	-0.5338940495165012
2216	98-99	-0.47323935576340403
2216	100	-0.43112144227968585
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	44185.0
3	8770.0
4	4831.0
5	3951.0
6	3471.0
7	3888.0
8	4512.0
9	5114.0
10	5682.0
11	6305.0
12	7200.0
13	7846.0
14	8474.0
15	8750.0
16	8914.0
17	8754.0
18	8654.0
19	8548.0
20	8784.0
21	8934.0
22	10517.0
23	14884.0
24	22892.0
25	30223.0
26	30793.0
27	27284.0
28	29281.0
29	36216.0
30	44010.0
31	53993.0
32	70213.0
33	93742.0
34	133406.0
35	203717.0
36	364401.0
37	766237.0
38	1279547.0
39	551944.0
40	905.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.216750332398256	20.22937067199689	13.9097781598788	23.64410083572605
2	15.035885580558203	20.490102771102205	40.73970714603492	23.73430450230468
3	17.63050613635602	26.01767205517387	30.390987617574126	25.960834190895987
4	12.001721215865262	17.329666689697497	39.99230838010624	30.676303714331
5	12.674211402669503	38.21467387300231	36.19570414459146	12.915410579736728
6	30.01881749318066	39.00223335469895	18.23302544997994	12.745923702140448
7	26.426288182847806	32.84711922331754	23.40062284591499	17.325969747919657
8	24.38818393865324	36.85567350838233	21.03525202888461	17.720890524079824
9	24.46133426291136	17.468283739464756	21.70997470423056	36.36040729339332
10-11	23.235636237609473	27.48143390143681	29.453983415267754	19.82894644568597
12-13	24.046335087680294	24.79896581405616	29.25929290394712	21.895406194316426
14-15	21.388607192560606	26.770398957383374	26.29742016418315	25.543573685872868
16-17	21.138845395308383	29.978898698511756	26.88538744397626	21.996868462203597
18-19	21.10235354214044	29.068124960062462	28.715671026226087	21.113850471571016
20-21	22.306891824463097	28.396688180435177	28.08094288638149	21.21547710872024
22-23	21.907484833188885	28.159328501541285	28.11847731883161	21.81470934643822
24-25	21.828168486660257	28.238734921979873	28.114930929101174	21.818165662258696
26-27	21.601602650333657	28.158345636831257	28.14232523650434	22.09772647633075
28-29	21.619752715151268	28.170341286313512	28.255492372024776	21.954413626510448
30-31	21.931675223933674	28.637562433121545	27.816991969822535	21.613770373122243
32-33	21.522105267703388	28.315256728765583	28.283759149697453	21.87887885383358
34-35	21.690162978650537	28.0760501874674	28.359526640528575	21.874260193353486
36-37	21.9825119975742	28.3882909466027	27.827680372484142	21.80151668333896
38-39	21.800191100284717	28.54425554144467	27.95929510590977	21.696258252360842
40-41	21.637225827528233	28.239161261247986	28.270661468793712	21.85295144243007
42-43	21.614447692055823	28.138717331944367	28.21216643874963	22.03466853725018
44-45	21.734390945062145	28.277934875722575	28.14250960315429	21.84516457606098
46-47	21.743095482960236	28.43229190680266	28.02893329893792	21.795679311299185
48-49	21.701829321729722	28.325782602455607	28.0215052944023	21.950882781412375
50-51	21.75391752206783	28.1946396595998	28.045181793623936	22.00626102470843
52-53	21.807937072822416	28.092742194622407	28.05499523590706	22.044325496648113
54-55	21.833835479838196	28.537808812480662	27.87532847798048	21.75302722970066
56-57	21.67365107135904	28.246001922028924	27.973750474823948	22.10659653178809
58-59	21.545636426949688	27.868949683894023	28.35415095619168	22.231262932964604
60-61	21.808175457676644	28.308950242340856	27.977053877822005	21.905820422160495
62-63	21.718137573029267	28.334149108865773	27.948380840087083	21.999332478017877
64-65	21.66899853458706	28.038255418211634	28.109500470585637	22.183245576615672
66-67	21.53375693220674	28.111244487635183	27.964027961995985	22.39097061816209
68-69	21.943081442433947	28.177001161901273	27.950665994595237	21.92925140106955
70-71	21.839888951352975	28.02496384963225	28.020951022299514	22.114196176715264
72-73	21.658151810621746	28.152156024535792	28.154581463248128	22.035110701594338
74-75	21.70300145339147	28.233611884157906	27.957181779334668	22.10620488311596
76-77	21.6929341080128	28.216177984110647	27.912244756723258	22.178643151153288
78-79	21.85645645035541	28.239119890087842	27.96595211913584	21.938471540420906
80-81	21.789768056044924	28.03923398501109	28.07775693340063	22.093241025543357
82-83	21.617192475968057	28.12435558435991	28.22103697714083	22.037414962531198
84-85	21.626812798996056	28.077097068630874	28.061569175494267	22.2345209568788
86-87	21.76388450158603	28.182574885758022	27.95718883978495	22.096351772870996
88-89	21.587740284355135	28.00417260390468	28.07477828280539	22.333308828934793
90-91	21.551341395583627	27.899750916052273	28.19037636319154	22.358531325172564
92-93	21.734923501491576	28.084101636417007	27.994617223057926	22.18635763903349
94-95	21.502889101932464	27.8504068459356	28.20489967209318	22.441804380038757
96-97	21.532759553020675	27.867785312698505	28.199245080255135	22.40021005402568
98-99	21.464020607247477	27.95816539835126	28.114055446984686	22.463758547416575
100	21.636484149416795	27.814922886652393	28.032358909112148	22.51623405481866
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	488.0
1	434.0
2	382.5
3	390.0
4	387.0
5	395.5
6	408.0
7	394.0
8	340.0
9	300.5
10	285.5
11	267.5
12	294.5
13	319.5
14	335.5
15	400.5
16	525.0
17	688.5
18	946.0
19	1357.0
20	2025.0
21	3016.0
22	4590.0
23	7303.5
24	10916.0
25	15554.0
26	22113.0
27	30812.5
28	41275.0
29	52811.0
30	65479.5
31	78877.5
32	92043.0
33	104800.5
34	116430.0
35	125841.0
36	133317.0
37	140355.0
38	145578.0
39	147488.0
40	148156.5
41	147343.5
42	146176.5
43	144901.5
44	141296.5
45	138629.5
46	135391.5
47	129630.0
48	125496.5
49	122334.5
50	118162.5
51	113875.5
52	107528.5
53	101665.0
54	97414.0
55	92413.5
56	85014.5
57	77176.5
58	69922.5
59	61514.5
60	53297.0
61	45370.5
62	38496.5
63	32245.0
64	26315.5
65	21232.5
66	16835.0
67	12956.5
68	10327.5
69	8129.0
70	6013.0
71	4364.5
72	3210.0
73	2352.0
74	1636.5
75	1063.0
76	665.5
77	467.0
78	347.0
79	228.5
80	136.5
81	86.5
82	52.0
83	37.5
84	27.5
85	22.0
86	17.5
87	10.0
88	7.0
89	7.0
90	5.5
91	3.5
92	2.5
93	2.5
94	4.0
95	4.0
96	2.0
97	1.5
98	3.5
99	3.5
100	10.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.06287165856298284
2	0.04314970510983884
3	0.05726219689870378
4	0.0766541820186549
5	0.0915281391918111
6	0.08987829752584667
7	0.09254342637086613
8	0.10437152200685725
9	0.0999042584190151
10-11	0.10416846457104625
12-13	0.10938450245344146
14-15	0.10886416777417576
16-17	0.07736488304399342
18-19	0.09996771386770606
20-21	0.09360947790887392
22-23	0.058620143500689885
24-25	0.022450537746854387
26-27	0.18493455966487402
28-29	0.06732623106108679
30-31	0.07765677810797174
32-33	0.0751693245192869
34-35	0.020826078260366337
36-37	0.05332795907986553
38-39	0.07857053656912126
40-41	0.08350737047727634
42-43	0.07697145926210959
44-45	0.07397636208389724
46-47	0.08123566541414072
48-49	0.07784714445404455
50-51	0.07301183926379495
52-53	0.07693338599289502
54-55	0.06787194791982887
56-57	0.07072744311092113
58-59	0.06708510035606122
60-61	0.0646991754852819
62-63	0.07165389266180885
64-65	0.06742775977899229
66-67	0.0688872350988839
68-69	0.06829075388118906
70-71	0.06087915747408733
72-73	0.05939429997471935
74-75	0.061666005037854986
76-77	0.05658956914257982
78-79	0.052845697669814395
80-81	0.047198162736320784
82-83	0.05320104818248366
84-85	0.04308624966114791
86-87	0.04162677434125629
88-89	0.04323854273800616
90-91	0.039126629662833284
92-93	0.04203288921287831
94-95	0.04087800004670321
96-97	0.04410153684020294
98-99	0.044164992288893874
100	0.052338054080286876
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3939772.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.34634836179939
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.56618525426423	44.39450643002499
2	14.94027698936341	18.031823196437998
3	5.2508706810420716	9.50612613962771
4	2.309429707253486	5.574625985240291
5	1.2584405586748968	3.7971146173206383
6	0.7375250603985962	2.6704166532222495
7	0.4591186120595956	1.9394292191913933
8	0.307585410005533	1.4849325042560635
9	0.2174268808912595	1.1808826467735067
>10	0.9078329925137041	9.062198934737395
>50	0.034636802143708637	1.3963254368077171
>100	0.010628971009333993	0.9424203928992063
>500	4.208038020724296E-5	0.019197843460865383
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	2.538217947637579E-5	2.538217947637579E-5
6	0.0	0.0	0.0	2.538217947637579E-5	2.538217947637579E-5
7	0.0	0.0	0.0	2.538217947637579E-5	2.538217947637579E-5
8	0.0	0.0	0.0	2.538217947637579E-5	2.538217947637579E-5
9	2.538217947637579E-5	0.0	0.0	5.076435895275158E-5	2.538217947637579E-5
10-11	2.538217947637579E-5	0.0	0.0	1.6498416659644263E-4	2.538217947637579E-5
12-13	2.538217947637579E-5	0.0	0.0	2.0305743581100632E-4	2.538217947637579E-5
14-15	2.538217947637579E-5	0.0	0.0	2.4113070502557003E-4	2.538217947637579E-5
16-17	2.538217947637579E-5	0.0	0.0	3.045861537165095E-4	2.538217947637579E-5
18-19	2.538217947637579E-5	0.0	0.0	3.045861537165095E-4	2.538217947637579E-5
20-21	2.538217947637579E-5	0.0	0.0	3.426594229310731E-4	2.538217947637579E-5
22-23	2.538217947637579E-5	0.0	0.0	3.6804160240744895E-4	2.538217947637579E-5
24-25	2.538217947637579E-5	0.0	0.0	4.5687923057476424E-4	2.538217947637579E-5
26-27	2.538217947637579E-5	0.0	0.0	5.964812176948311E-4	5.076435895275158E-5
28-29	2.538217947637579E-5	0.0	0.0	0.0011168158969605347	5.076435895275158E-5
30-31	2.538217947637579E-5	0.0	0.0	0.003413903139572544	5.076435895275158E-5
32-33	2.538217947637579E-5	0.0	0.0	0.00898529153463703	5.076435895275158E-5
34-35	2.538217947637579E-5	0.0	0.0	0.01725988204393554	5.076435895275158E-5
36-37	2.538217947637579E-5	0.0	0.0	0.031194698576465847	5.076435895275158E-5
38-39	2.538217947637579E-5	0.0	0.0	0.056183454270957806	5.076435895275158E-5
40-41	2.538217947637579E-5	0.0	0.0	0.08495415470742977	5.076435895275158E-5
42-43	2.538217947637579E-5	0.0	0.0	0.11580619386096455	5.076435895275158E-5
44-45	2.538217947637579E-5	0.0	0.0	0.1515316114739635	5.076435895275158E-5
46-47	2.538217947637579E-5	0.0	0.0	0.19576005921154827	5.076435895275158E-5
48-49	2.538217947637579E-5	0.0	0.0	0.24863113906083906	5.076435895275158E-5
50-51	2.538217947637579E-5	0.0	0.0	0.3047511378831059	5.076435895275158E-5
52-53	2.538217947637579E-5	0.0	0.0	0.362508287281599	5.076435895275158E-5
54-55	2.538217947637579E-5	0.0	0.0	0.4271313162284518	5.076435895275158E-5
56-57	2.538217947637579E-5	0.0	0.0	0.5025671536322407	5.076435895275158E-5
58-59	2.538217947637579E-5	0.0	0.0	0.5883589202623909	5.076435895275158E-5
60-61	2.538217947637579E-5	0.0	0.0	0.6841005012472803	5.076435895275158E-5
62-63	2.538217947637579E-5	0.0	0.0	0.7801720505653627	5.076435895275158E-5
64-65	2.538217947637579E-5	0.0	0.0	0.8854827132128458	5.076435895275158E-5
66-67	2.538217947637579E-5	0.0	0.0	1.0071141172636386	5.076435895275158E-5
68-69	2.538217947637579E-5	0.0	0.0	1.1453835399611956	5.076435895275158E-5
70-71	2.538217947637579E-5	0.0	0.0	1.2957094979100314	5.076435895275158E-5
72-73	2.538217947637579E-5	0.0	0.0	1.4570893950208286	5.076435895275158E-5
74-75	2.538217947637579E-5	0.0	0.0	1.6217309021943402	5.076435895275158E-5
76-77	2.538217947637579E-5	0.0	0.0	1.8051171489111553	5.076435895275158E-5
78-79	2.538217947637579E-5	0.0	0.0	2.0148754801039246	5.076435895275158E-5
80-81	2.538217947637579E-5	0.0	0.0	2.242579012186492	5.076435895275158E-5
82-83	2.538217947637579E-5	0.0	0.0	2.48400161227604	5.076435895275158E-5
84-85	2.538217947637579E-5	0.0	0.0	2.7286731313385646	5.076435895275158E-5
86-87	2.538217947637579E-5	0.0	0.0	2.9916705839830326	5.076435895275158E-5
88	2.538217947637579E-5	0.0	0.0	3.208459778890758	5.076435895275158E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	4605	0.0	36.342537	1
TCAACGC	4420	0.0	35.307014	4
ATCAACG	4595	0.0	33.860054	3
CAACGCA	4670	0.0	33.517994	5
GGTATCA	2935	0.0	32.995445	1
AACGCAG	4950	0.0	31.90083	6
TATCAAC	5605	0.0	28.764954	2
ACGCAGA	5995	0.0	26.10529	7
GTACATG	9665	0.0	25.730526	1
CGCAGAG	6265	0.0	24.851362	8
TACATGG	9815	0.0	24.328682	2
ACATGGG	10010	0.0	22.446001	3
CATGGGG	7435	0.0	19.914745	4
GCAGAGT	7760	0.0	18.79334	9
TATACTG	2480	0.0	18.385302	5
TAGACCG	135	0.009988008	17.409525	5
CCTACAC	1460	0.0	17.063503	3
GAGTACA	7485	0.0	16.957695	1
AGAGTAC	6515	0.0	16.397633	10-11
GTATAGG	1420	0.0	16.221931	1
>>END_MODULE