FastQCFastQC Report
Wed 25 May 2016
SRR1033080_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033080_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3939772
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60350.1531814531399279No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50490.12815462417622137No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA49750.053.548471
GTATCAA98050.045.2034871
TCAACGC116400.037.3071334
ATCAACG116950.037.1330953
CAACGCA118100.036.7303165
AACGCAG122700.035.4682166
TATCAAC125050.034.974052
ACGCAGA142150.030.3176387
CGCAGAG143550.029.8255238
GCAGAGT162750.025.9600839
GTACATG98700.023.9816071
TACATGG98450.022.8819922
AGAGTAC150650.021.68118510-11
GTGGTAT25750.021.60711
ACATGGG102400.021.158773
GAGTACT115700.020.73635912-13
CAGAGTA161500.020.50103810-11
AGTACTT122300.019.48281112-13
GTACTTT127050.018.62506114-15
CATGGGG74650.018.5722754
Produced by FastQC (version 0.11.5)