##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1033080_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3939772
Sequences flagged as poor quality	0
Sequence length	100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.74379304183085	34.0	31.0	34.0	31.0	34.0
2	32.74479335352401	34.0	31.0	34.0	31.0	34.0
3	32.844420438543146	34.0	31.0	34.0	31.0	34.0
4	36.32616633652912	37.0	37.0	37.0	35.0	37.0
5	36.31129644050468	37.0	37.0	37.0	35.0	37.0
6	36.37839118608894	37.0	37.0	37.0	35.0	37.0
7	36.330792492560484	37.0	37.0	37.0	35.0	37.0
8	36.33507015126764	37.0	37.0	37.0	35.0	37.0
9	38.13897910843572	39.0	39.0	39.0	37.0	39.0
10-11	38.10577071465049	39.0	39.0	39.0	37.0	39.0
12-13	38.08188126114912	39.0	39.0	39.0	35.0	39.0
14-15	39.58738767116473	41.0	40.0	41.0	37.0	41.0
16-17	39.593799336611355	41.0	40.0	41.0	37.0	41.0
18-19	39.611510767628175	41.0	40.0	41.0	37.0	41.0
20-21	39.60405500622879	41.0	40.0	41.0	37.0	41.0
22-23	39.53638903469541	41.0	39.5	41.0	37.0	41.0
24-25	39.485821006900906	41.0	39.0	41.0	36.5	41.0
26-27	39.381630459833715	41.0	39.0	41.0	36.0	41.0
28-29	39.33114695469687	41.0	39.0	41.0	36.0	41.0
30-31	39.32792037204183	41.0	39.0	41.0	36.0	41.0
32-33	39.28547210346183	41.0	39.0	41.0	35.5	41.0
34-35	39.20797878658968	41.0	39.0	41.0	35.0	41.0
36-37	39.120581470196754	41.0	39.0	41.0	35.0	41.0
38-39	39.030460772856905	41.0	39.0	41.0	35.0	41.0
40-41	38.929661411878655	41.0	39.0	41.0	35.0	41.0
42-43	38.813984413311225	41.0	39.0	41.0	35.0	41.0
44-45	38.65506607996605	41.0	39.0	41.0	35.0	41.0
46-47	38.456635561651794	41.0	38.5	41.0	35.0	41.0
48-49	38.255389651990015	41.0	38.0	41.0	34.0	41.0
50-51	38.12209577102431	40.0	38.0	41.0	34.0	41.0
52-53	37.995184365998846	40.0	38.0	41.0	34.0	41.0
54-55	37.85354266693606	40.0	38.0	41.0	34.0	41.0
56-57	37.70807777708964	40.0	37.0	41.0	33.0	41.0
58-59	37.53626491583777	40.0	37.0	41.0	33.0	41.0
60-61	37.34952606394482	40.0	36.5	41.0	33.0	41.0
62-63	37.126956966037625	39.5	36.0	41.0	33.0	41.0
64-65	36.868051501457444	39.0	35.5	41.0	32.5	41.0
66-67	36.59368384769474	39.0	35.0	41.0	32.0	41.0
68-69	36.29112941053442	38.5	35.0	40.5	32.0	41.0
70-71	35.967148987301805	37.0	35.0	40.0	31.5	41.0
72-73	35.625096579192906	37.0	35.0	39.5	31.0	41.0
74-75	35.27877856891211	36.5	35.0	39.0	31.0	41.0
76-77	34.91952846509899	36.0	35.0	39.0	31.0	40.5
78-79	34.55625376290811	35.5	35.0	37.5	31.0	39.5
80-81	34.214139168459496	35.0	34.5	37.0	30.0	39.0
82-83	33.90837743910053	35.0	34.0	37.0	30.0	39.0
84-85	33.67623583293653	35.0	34.0	36.0	30.0	38.0
86-87	33.458699005932324	35.0	34.0	36.0	30.0	37.0
88-89	33.242865196260084	35.0	34.0	36.0	30.0	37.0
90-91	33.05564522515516	35.0	34.0	35.0	30.0	36.5
92-93	32.88154822157222	35.0	34.0	35.0	30.0	36.0
94-95	32.727477503774324	35.0	34.0	35.0	29.5	36.0
96-97	32.58783744338505	35.0	34.0	35.0	29.0	36.0
98-99	32.46592417023117	35.0	34.0	35.0	29.0	36.0
100	32.365804670930196	35.0	34.0	35.0	29.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.10921011612087739
1101	2	0.08872486553926251
1101	3	0.15140171242655498
1101	4	0.11389101386834
1101	5	0.08601092190959747
1101	6	0.07570096263333426
1101	7	0.13911186444706658
1101	8	0.1744414016688154
1101	9	0.2290369954562479
1101	10-11	0.23212712670785152
1101	12-13	0.23186357469423768
1101	14-15	0.3249982486905054
1101	16-17	0.3037373155771519
1101	18-19	0.3036309972681295
1101	20-21	0.2915649770052866
1101	22-23	0.31052126671646363
1101	24-25	0.35207916718597687
1101	26-27	0.32904011657032584
1101	28-29	0.36311370762183515
1101	30-31	0.3363301575296731
1101	32-33	0.37061807102211475
1101	34-35	0.3913383346352859
1101	36-37	0.3905818391467406
1101	38-39	0.3674872686746582
1101	40-41	0.4287382677482725
1101	42-43	0.4732818945698938
1101	44-45	0.4840464256124761
1101	46-47	0.4700324305959853
1101	48-49	0.44510589645633303
1101	50-51	0.440833597847444
1101	52-53	0.41689825806295033
1101	54-55	0.4552566545190757
1101	56-57	0.4655008570532999
1101	58-59	0.4541232506613113
1101	60-61	0.4667042617798174
1101	62-63	0.47009756702797745
1101	64-65	0.47326944145977734
1101	66-67	0.5059540142464911
1101	68-69	0.4405811877415644
1101	70-71	0.41976623926431955
1101	72-73	0.4185812707915062
1101	74-75	0.4204648805721476
1101	76-77	0.40533292760012074
1101	78-79	0.36661791555272316
1101	80-81	0.3632899615688814
1101	82-83	0.4255169554738103
1101	84-85	0.4058711614005759
1101	86-87	0.43252628479943667
1101	88-89	0.4335047186425882
1101	90-91	0.4118589089835538
1101	92-93	0.40685479168271854
1101	94-95	0.3988028610133867
1101	96-97	0.4679525391503958
1101	98-99	0.47856216564365184
1101	100	0.5116865102158528
1102	1	0.1488839282908927
1102	2	0.12627216182421108
1102	3	0.14995165640896602
1102	4	0.09071010390123746
1102	5	0.10822284504586577
1102	6	0.11712476529937277
1102	7	0.10654311711656561
1102	8	0.13217901239019625
1102	9	0.18323772737463884
1102	10-11	0.18393117808749082
1102	12-13	0.20311808349492821
1102	14-15	0.30016301698343284
1102	16-17	0.31358337064210673
1102	18-19	0.30166059825744185
1102	20-21	0.29334997849237965
1102	22-23	0.2620367045369463
1102	24-25	0.2704179522640544
1102	26-27	0.2581060317143056
1102	28-29	0.3124176527805105
1102	30-31	0.271934462609714
1102	32-33	0.3246249496685678
1102	34-35	0.36332807424855673
1102	36-37	0.3483499185150549
1102	38-39	0.2993430217142574
1102	40-41	0.3209761842742438
1102	42-43	0.3058916208328526
1102	44-45	0.2974297368443928
1102	46-47	0.29825977310382257
1102	48-49	0.2841229136149366
1102	50-51	0.2894163026135175
1102	52-53	0.27247274859362136
1102	54-55	0.23425365181994806
1102	56-57	0.291981493529363
1102	58-59	0.28669642852442223
1102	60-61	0.3161386910154107
1102	62-63	0.32750080531404535
1102	64-65	0.3388033360125391
1102	66-67	0.3228449935143942
1102	68-69	0.3046764869604459
1102	70-71	0.24186547033629324
1102	72-73	0.28558723426043287
1102	74-75	0.28029030640642816
1102	76-77	0.24362114311017535
1102	78-79	0.2665833378313991
1102	80-81	0.23175415727487803
1102	82-83	0.20752978976238268
1102	84-85	0.247199532910102
1102	86-87	0.20310862450081402
1102	88-89	0.21445415522419609
1102	90-91	0.1702953698349745
1102	92-93	0.2453520947881742
1102	94-95	0.29970428571613184
1102	96-97	0.29744887284972776
1102	98-99	0.2908020640293074
1102	100	0.2526932278536762
1103	1	0.18186819150639622
1103	2	0.1617280455074095
1103	3	0.17598251922174768
1103	4	0.11401729505838887
1103	5	0.16937790279687448
1103	6	0.14016982456517013
1103	7	0.17858970286967946
1103	8	0.16624066758524947
1103	9	0.21395124493932371
1103	10-11	0.23437066651464988
1103	12-13	0.22867760621797828
1103	14-15	0.2889066011197414
1103	16-17	0.2946645281004123
1103	18-19	0.28236473740770407
1103	20-21	0.30806558773581827
1103	22-23	0.270359870920835
1103	24-25	0.24597816047493382
1103	26-27	0.2769277130761765
1103	28-29	0.32165490374849526
1103	30-31	0.30736398525345976
1103	32-33	0.25627053915248865
1103	34-35	0.3373859048247283
1103	36-37	0.33615162892816386
1103	38-39	0.30821578804879124
1103	40-41	0.27633615812893453
1103	42-43	0.2915817431010339
1103	44-45	0.3392045750591848
1103	46-47	0.42376683248601665
1103	48-49	0.36650747914401194
1103	50-51	0.4266451535329523
1103	52-53	0.4221965305993578
1103	54-55	0.3968783484411773
1103	56-57	0.4100370817538632
1103	58-59	0.41435748221061885
1103	60-61	0.41746896416081114
1103	62-63	0.3632107059223415
1103	64-65	0.38201408642413526
1103	66-67	0.37948540823722254
1103	68-69	0.36683242415202244
1103	70-71	0.3031389427501807
1103	72-73	0.38723286541125645
1103	74-75	0.35991195274121424
1103	76-77	0.300634711871389
1103	78-79	0.28762867115791835
1103	80-81	0.2956110955609432
1103	82-83	0.322776406779127
1103	84-85	0.32099034917423097
1103	86-87	0.26074136686746385
1103	88-89	0.2964038056052445
1103	90-91	0.29030590887438024
1103	92-93	0.35819285112587806
1103	94-95	0.36987325645078073
1103	96-97	0.34666787371222796
1103	98-99	0.402755735266922
1103	100	0.3997052334338136
1104	1	0.07012280901079748
1104	2	0.024315504262631293
1104	3	0.09493571158294145
1104	4	0.0377862125768047
1104	5	0.05612596534261627
1104	6	0.08055907466204815
1104	7	0.09524928064821125
1104	8	0.1068141751512286
1104	9	0.1016947225080429
1104	10-11	0.12559164588283522
1104	12-13	0.11725064456604883
1104	14-15	0.17900096204972016
1104	16-17	0.18147442805764058
1104	18-19	0.18077106634129336
1104	20-21	0.16815443863713853
1104	22-23	0.17339813405787652
1104	24-25	0.19226109179216166
1104	26-27	0.20091967318313664
1104	28-29	0.2161949949178137
1104	30-31	0.16647749737777673
1104	32-33	0.1870827925347882
1104	34-35	0.1238732592992875
1104	36-37	0.16164811063039508
1104	38-39	0.12026713703739489
1104	40-41	0.16771293753101446
1104	42-43	0.13786701664696466
1104	44-45	0.10163794862651798
1104	46-47	0.06243097001756581
1104	48-49	0.013593392736105159
1104	50-51	0.06040968966807725
1104	52-53	0.0751826083662408
1104	54-55	0.07491752208745339
1104	56-57	0.11394245532839875
1104	58-59	0.02595052332046066
1104	60-61	0.10871280599756261
1104	62-63	0.045670562760690814
1104	64-65	0.06461541001500137
1104	66-67	0.05001037118303486
1104	68-69	0.07822113798147967
1104	70-71	0.0768081932897644
1104	72-73	0.04635130329400283
1104	74-75	0.03137718538050649
1104	76-77	0.0033362345903071855
1104	78-79	-8.917088699718079E-4
1104	80-81	0.052335061463750776
1104	82-83	-0.005158228824782896
1104	84-85	0.04598292353708899
1104	86-87	0.07248970610505978
1104	88-89	0.08340376390842863
1104	90-91	0.10031131466099197
1104	92-93	0.0640707802147773
1104	94-95	0.09464835423560913
1104	96-97	0.11623877098014646
1104	98-99	0.11805125760773194
1104	100	0.154052223290293
1105	1	0.022615698411946994
1105	2	0.0625858643471986
1105	3	0.07285278328372158
1105	4	0.03229439389596678
1105	5	0.04251595610512737
1105	6	0.08824979086099205
1105	7	0.09916128134656788
1105	8	0.10694215853261824
1105	9	0.10017790983182095
1105	10-11	0.08993304685451875
1105	12-13	0.13602030752341676
1105	14-15	0.19025776625289836
1105	16-17	0.17584106320933302
1105	18-19	0.15700203713593197
1105	20-21	0.17070177645950224
1105	22-23	0.18265911264001033
1105	24-25	0.19343729359063389
1105	26-27	0.18255411960198842
1105	28-29	0.2048354834345787
1105	30-31	0.1946521510458723
1105	32-33	0.21817991835848005
1105	34-35	0.22502914157824705
1105	36-37	0.2480684573741243
1105	38-39	0.23024197437027283
1105	40-41	0.21865790615658653
1105	42-43	0.17588566375165726
1105	44-45	0.15921296193037904
1105	46-47	0.2108162340258346
1105	48-49	0.21893009013358267
1105	50-51	0.19499753139479736
1105	52-53	0.22127964541532918
1105	54-55	0.21245126260028258
1105	56-57	0.1790311904728341
1105	58-59	0.165269003183667
1105	60-61	0.15619260607716257
1105	62-63	0.19085069382158792
1105	64-65	0.147384778043417
1105	66-67	0.16315594130583833
1105	68-69	0.1871695190040299
1105	70-71	0.1434191662248594
1105	72-73	0.13389884085921722
1105	74-75	0.19070132955637575
1105	76-77	0.14908840697873416
1105	78-79	0.1766270977824007
1105	80-81	0.1944479684521383
1105	82-83	0.24304254430333572
1105	84-85	0.20253378620426332
1105	86-87	0.19510487511956853
1105	88-89	0.2147447979451016
1105	90-91	0.24339285117176956
1105	92-93	0.26294357706347427
1105	94-95	0.18488791989108222
1105	96-97	0.18547808855993253
1105	98-99	0.15123039439311725
1105	100	0.20784642502662365
1106	1	-0.004096486427357604
1106	2	0.01692363642087713
1106	3	0.04565731239136994
1106	4	0.022907949274681982
1106	5	0.033515150903213
1106	6	0.014231875707316988
1106	7	0.04421084437493761
1106	8	0.04672975766928289
1106	9	0.010830768864003915
1106	10-11	0.06522544492862892
1106	12-13	0.06010264118608433
1106	14-15	0.08165232979717985
1106	16-17	0.09216190295624216
1106	18-19	0.13205479458734715
1106	20-21	0.09443027358711475
1106	22-23	0.06589929885885937
1106	24-25	0.07840773339861329
1106	26-27	0.12752959368876304
1106	28-29	0.06728064701682968
1106	30-31	0.06314669437765019
1106	32-33	0.06257270471046894
1106	34-35	0.09627251421052563
1106	36-37	0.08685457190502888
1106	38-39	0.14187365550842657
1106	40-41	0.11902335663384633
1106	42-43	0.06850251814957176
1106	44-45	0.14365475534920336
1106	46-47	0.17848365500980634
1106	48-49	0.1738530941727845
1106	50-51	0.11021386874848815
1106	52-53	0.10934450586662336
1106	54-55	0.13438643041821763
1106	56-57	0.07834914817170358
1106	58-59	0.070453238470364
1106	60-61	0.08249878587229631
1106	62-63	0.08468347849627378
1106	64-65	0.09476390234746646
1106	66-67	0.06384955043379392
1106	68-69	0.07179913790182724
1106	70-71	0.09715916186196694
1106	72-73	0.11580559103319388
1106	74-75	0.16081368561297893
1106	76-77	0.11258646982972209
1106	78-79	0.12443349972657103
1106	80-81	0.12288535375562759
1106	82-83	0.10744287853987089
1106	84-85	0.11520059233247082
1106	86-87	0.06428877541587497
1106	88-89	0.0431167046434453
1106	90-91	0.035253220961571685
1106	92-93	0.07425889017582676
1106	94-95	0.0389807037459633
1106	96-97	0.02742947349310043
1106	98-99	0.0438044915799054
1106	100	0.03689555647807907
1107	1	-0.01368282549758959
1107	2	-0.043238655619589395
1107	3	-0.014322333336771464
1107	4	-0.015920058762432632
1107	5	0.004364861447413659
1107	6	0.015583379521586949
1107	7	0.01170329478739518
1107	8	0.0036061236422213483
1107	9	0.01591840697161473
1107	10-11	0.03305213640197735
1107	12-13	1.0742740987979005E-4
1107	14-15	0.046821985172165625
1107	16-17	-0.008642152208921061
1107	18-19	0.03629435024454608
1107	20-21	0.0111774526568027
1107	22-23	0.020792126443559766
1107	24-25	0.05019554211511945
1107	26-27	-0.007607347088637084
1107	28-29	0.04153115187242662
1107	30-31	0.01716706318907768
1107	32-33	0.008891482656174787
1107	34-35	0.05281621706047446
1107	36-37	0.03062009134696808
1107	38-39	0.053619151820058164
1107	40-41	0.03150229820720085
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2216	14-15	-0.3080648996532105
2216	16-17	-0.2734698937376123
2216	18-19	-0.24713949574044847
2216	20-21	-0.28442324586453793
2216	22-23	-0.3246040908751695
2216	24-25	-0.2946956988007088
2216	26-27	-0.2813752829297087
2216	28-29	-0.3082125813790455
2216	30-31	-0.3012310842156438
2216	32-33	-0.30456611373579534
2216	34-35	-0.2756450540634816
2216	36-37	-0.2941762768466276
2216	38-39	-0.280213951815675
2216	40-41	-0.2613434101919978
2216	42-43	-0.28694163362303016
2216	44-45	-0.24787890457494655
2216	46-47	-0.22017707799958686
2216	48-49	-0.27280568483394063
2216	50-51	-0.32293180031581414
2216	52-53	-0.3356117762257469
2216	54-55	-0.2436960949261504
2216	56-57	-0.30420908432840577
2216	58-59	-0.21544912655441806
2216	60-61	-0.2992160855185233
2216	62-63	-0.3060053692338158
2216	64-65	-0.39121627826858685
2216	66-67	-0.3718741345650969
2216	68-69	-0.36663180319499133
2216	70-71	-0.3219494152286444
2216	72-73	-0.30634595763937256
2216	74-75	-0.3371255867700427
2216	76-77	-0.29278599835501495
2216	78-79	-0.4048941874202967
2216	80-81	-0.38588874735820866
2216	82-83	-0.3102831892338429
2216	84-85	-0.39349716299853554
2216	86-87	-0.4244527259082531
2216	88-89	-0.45019444987330104
2216	90-91	-0.4459931934547612
2216	92-93	-0.4092025349354671
2216	94-95	-0.3623737575065178
2216	96-97	-0.4631329519866796
2216	98-99	-0.5292570183121867
2216	100	-0.48756196250024075
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	29.0
3	4.0
4	10.0
5	34.0
6	80.0
7	197.0
8	452.0
9	874.0
10	1559.0
11	2565.0
12	4092.0
13	6643.0
14	7746.0
15	10168.0
16	12481.0
17	17846.0
18	21146.0
19	10748.0
20	7159.0
21	6563.0
22	6785.0
23	7249.0
24	8419.0
25	10462.0
26	13398.0
27	17424.0
28	22366.0
29	29425.0
30	38205.0
31	49258.0
32	63344.0
33	83399.0
34	116830.0
35	176975.0
36	320117.0
37	732975.0
38	1402176.0
39	728025.0
40	2544.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.92421057015386	20.404694132994003	14.167849815779176	23.503245481072955
2	15.307955734106432	20.74679421366922	40.26040676725802	23.684843284966334
3	17.67823809550401	26.135140060807494	30.65496240017504	25.531659443513455
4	12.094524740288962	17.48147749543299	39.647817950095785	30.776179814182264
5	12.396621214647901	37.942270072864865	36.206351708315154	13.454757004172082
6	29.90062112747162	39.04437592946392	17.944443979104367	13.110558963960086
7	26.136284028618917	33.02851027927504	23.410593303368824	17.424612388737216
8	24.720598108258002	36.0497289806092	20.91218870208934	18.317484209043457
9	24.83016783712357	16.503543859898492	21.860782806720795	36.80550549625715
10-11	23.475174928745425	26.92189371551016	29.385935188224018	20.216996167520403
12-13	24.56753842608151	24.329961226182633	29.52125148358839	21.58124886414747
14-15	21.652307721594894	26.026518095951218	27.118487472277835	25.20268671017606
16-17	20.784424461595766	29.4867146348103	27.587706634972747	22.141154268621182
18-19	20.626965302796073	28.080295845770127	30.339839616950847	20.952899234482953
20-21	21.782065505837775	27.404016629392924	29.922678247035172	20.89123961773413
22-23	21.309755452481188	27.236427239587325	29.98675555857886	21.467061749352627
24-25	21.223252581524704	27.40184215856636	29.93962016647858	21.435285093430355
26-27	21.027966751845128	27.347375194094575	29.983584056673397	21.6410739973869
28-29	21.032148118516744	27.376182938876543	30.108821239038686	21.48284770356803
30-31	21.41190529342016	27.771936346006115	29.607856400699685	21.20830195987404
32-33	21.0333745201499	27.514003348417116	30.049023141440674	21.403598989992314
34-35	21.212039681484107	27.33392947612197	30.02549386106607	21.428536981327852
36-37	21.5011680878995	27.654468329639382	29.447681236376116	21.396682346085
38-39	21.42269908004829	27.811177905726524	29.467162059124234	21.298960955100952
40-41	21.291930911186554	27.572107962153623	29.63948779236958	21.496473334290243
42-43	21.29667655894808	27.515069399955124	29.45165354746417	21.736600493632626
44-45	21.44553035048729	27.635482459391053	29.30665784720537	21.61232934291629
46-47	21.526169534683735	27.89618282479291	28.946167443192145	21.631480197331214
48-49	21.58872391600326	27.910967944337894	28.67787780612685	21.82243033353199
50-51	21.640935059186166	27.856967357501905	28.59571569116182	21.906381892150105
52-53	21.703552895954385	27.82596302527151	28.539164195288457	21.931319883485642
54-55	21.750129195293535	28.14863398186494	28.413141674188253	21.68809514865327
56-57	21.563240207808978	27.877564996146987	28.483932572747868	22.07526222329617
58-59	21.419399396716358	27.528217368923887	28.885605562961516	22.166777671398243
60-61	21.639247144250987	27.957315804061757	28.548022575925714	21.855414475761542
62-63	21.537794572883914	27.90133540722661	28.53194296522743	22.028927054662045
64-65	21.511320959690053	27.683987804370407	28.649830497805457	22.154860738134087
66-67	21.424742345496135	27.77287619689667	28.501154381522586	22.301227076084608
68-69	21.84133364333498	27.823100571506316	28.424748284415006	21.910817500743697
70-71	21.783547599143755	27.680044388361104	28.462018672910343	22.074389339584798
72-73	21.63204207365555	27.87306071503132	28.490141842486498	22.004755368826633
74-75	21.707569873712124	27.90830222638716	28.30515295505307	22.078974944847648
76-77	21.70737291396558	27.914381847477472	28.22594810054998	22.152297138006972
78-79	21.86160772755378	27.96141502604719	28.236037516891844	21.94093972950719
80-81	21.771056802271808	27.818792559569438	28.319075317048803	22.09107532110995
82-83	21.63300690979598	27.902803438475708	28.43758098507806	22.02660866665025
84-85	21.62606110201301	27.8254934549512	28.2454289232981	22.30301651973769
86-87	21.710489244241383	27.930223998984705	28.192055333460246	22.167231423313662
88-89	21.496881977096592	27.809939192969168	28.277845690863916	22.41533313907032
90-91	21.434642435290836	27.752227937991496	28.355918257942164	22.457211368775504
92-93	21.5856074967279	27.92945635701595	28.170537919264078	22.314398226992076
94-95	21.36206612094676	27.70015863950758	28.281946824037057	22.655828415508598
96-97	21.31582450782921	27.72582530610007	28.301011824180904	22.657338361889813
98-99	21.296901238854673	27.787194575924268	28.23628193335242	22.67962225186864
100	21.44410894843661	27.64500077669469	28.087995955095877	22.822894319772818
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	23.0
1	25.0
2	92.0
3	170.5
4	124.0
5	90.5
6	239.0
7	559.5
8	973.0
9	1170.0
10	1034.0
11	936.0
12	1052.0
13	1227.0
14	1491.0
15	1814.5
16	2082.0
17	2393.0
18	2873.5
19	3443.0
20	4112.0
21	5172.0
22	6876.5
23	9516.5
24	12999.0
25	17524.5
26	23799.5
27	32172.0
28	42384.5
29	53714.0
30	65709.0
31	78714.0
32	91735.0
33	103983.0
34	115293.5
35	124403.0
36	131851.0
37	139026.0
38	144136.0
39	145556.5
40	146481.0
41	146884.0
42	145660.5
43	144398.0
44	141599.0
45	138464.5
46	134836.5
47	129125.0
48	125166.5
49	122001.5
50	117436.0
51	112747.5
52	106232.0
53	100473.0
54	96416.5
55	91473.5
56	84165.5
57	76855.0
58	69511.5
59	60950.0
60	52915.5
61	45024.0
62	37954.5
63	31512.0
64	25805.5
65	20747.5
66	16244.5
67	12405.5
68	9762.5
69	7740.0
70	5658.0
71	4021.0
72	2950.0
73	2048.0
74	1376.0
75	891.0
76	529.0
77	336.5
78	229.0
79	133.0
80	68.5
81	35.0
82	14.0
83	6.5
84	2.5
85	1.0
86	1.5
87	1.0
88	1.0
89	1.0
90	0.5
91	0.0
92	0.0
93	0.5
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.04657629933914957
2	0.24399889130640048
3	0.00319815461402335
4	0.002665128845019458
5	3.2996833319288527E-4
6	2.538217947637579E-5
7	0.0
8	4.314970510983884E-4
9	0.0
10-11	6.345544869093947E-4
12-13	0.0
14-15	2.4113070502557003E-4
16-17	1.1421980764369106E-4
18-19	4.1880596136020055E-4
20-21	2.538217947637579E-5
22-23	1.5229307685825475E-4
24-25	0.0011675802559132863
26-27	1.1421980764369106E-4
28-29	2.0305743581100632E-4
30-31	8.883762816731526E-5
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	6.345544869093947E-5
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	2.0305743581100632E-4
70-71	1.2691089738187895E-5
72-73	3.6804160240744895E-4
74-75	8.883762816731526E-5
76-77	0.0
78-79	0.0
80-81	0.0
82-83	7.614653842912737E-5
84-85	0.0
86-87	5.584079484802674E-4
88-89	0.002436689229732076
90-91	0.002195558524706506
92-93	6.726277561239584E-4
94-95	5.584079484802674E-4
96-97	1.6498416659644263E-4
98-99	1.0152871790550316E-4
100	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3939772.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.06866356191519
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.80129117078141	41.69405020307217
2	15.775835842181571	18.321634078552893
3	5.6808879439495925	9.89644712250447
4	2.526971511498886	5.869514341270925
5	1.3448268388965412	3.904614862845859
6	0.7814222117037195	2.7225686106738567
7	0.4943874985795364	2.0095894926962345
8	0.3235895617500815	1.503233071473045
9	0.23515954801452996	1.2289860609325003
>10	0.9824931306224237	9.531860321903016
>50	0.038591015005024216	1.5029571311854184
>100	0.01397495603323191	1.2811716423249009
>500	3.9376454333642355E-4	0.14872659580917094
>1k	8.750322000416082E-5	0.10304663547215888
>5k	8.750322000416082E-5	0.28159982928338484
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6035	0.1531814531399279	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5049	0.12815462417622137	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	5.076435895275158E-5	0.0
5	0.0	0.0	0.0	7.614653842912737E-5	0.0
6	0.0	0.0	0.0	7.614653842912737E-5	0.0
7	0.0	0.0	0.0	7.614653842912737E-5	0.0
8	0.0	0.0	0.0	7.614653842912737E-5	0.0
9	0.0	0.0	0.0	1.2691089738187895E-4	0.0
10-11	0.0	0.0	0.0	2.0305743581100632E-4	0.0
12-13	0.0	0.0	0.0	2.538217947637579E-4	0.0
14-15	0.0	0.0	0.0	2.918950639783216E-4	0.0
16-17	0.0	0.0	0.0	3.045861537165095E-4	0.0
18-19	0.0	0.0	0.0	3.045861537165095E-4	0.0
20-21	0.0	0.0	0.0	3.8073269214563687E-4	0.0
22-23	0.0	0.0	0.0	5.203346792657037E-4	0.0
24-25	0.0	0.0	0.0	6.599366663857705E-4	0.0
26-27	0.0	0.0	0.0	7.487742945530857E-4	0.0
28-29	0.0	0.0	0.0	0.0012944911532951652	0.0
30-31	0.0	0.0	0.0	0.003591578395907174	0.0
32-33	0.0	0.0	0.0	0.009201040060186225	0.0
34-35	0.0	0.0	0.0	0.01743755730027017	0.0
36-37	0.0	0.0	0.0	0.03143582928149142	0.0
38-39	0.0	2.538217947637579E-5	0.0	0.05658956914257983	0.0
40-41	0.0	2.538217947637579E-5	0.0	0.08541103393800453	0.0
42-43	0.0	2.538217947637579E-5	0.0	0.1164915127068267	0.0
44-45	0.0	2.538217947637579E-5	0.0	0.15236922339668385	0.0
46-47	0.0	2.538217947637579E-5	0.0	0.19667381767269781	0.0
48-49	0.0	2.538217947637579E-5	0.0	0.24969719059884685	0.0
50-51	0.0	2.538217947637579E-5	0.0	0.3060329379466629	0.0
52-53	0.0	2.538217947637579E-5	0.0	0.3638789249733233	0.0
54-55	0.0	2.538217947637579E-5	0.0	0.42889537770205993	0.0
56-57	0.0	2.538217947637579E-5	0.0	0.5047627121569471	0.0
58-59	0.0	2.538217947637579E-5	0.0	0.5913032530816504	0.0
60-61	0.0	2.538217947637579E-5	0.0	0.6874890222073764	0.0
62-63	0.0	2.538217947637579E-5	0.0	0.7842331992815827	0.0
64-65	0.0	2.538217947637579E-5	0.0	0.8900768876980698	0.0
66-67	0.0	2.538217947637579E-5	0.0	1.0123936105947247	0.0
68-69	0.0	2.538217947637579E-5	0.0	1.1520717442532207	0.0
70-71	0.0	2.538217947637579E-5	0.0	1.3036668111758751	0.0
72-73	0.0	2.538217947637579E-5	0.0	1.4664554192476111	0.0
74-75	0.0	2.538217947637579E-5	0.0	1.631985302702796	0.0
76-77	0.0	2.538217947637579E-5	0.0	1.817046773265052	0.0
78-79	0.0	2.538217947637579E-5	0.0	2.0287722233672403	0.0
80-81	0.0	2.538217947637579E-5	0.0	2.2581509792952486	0.0
82-83	0.0	2.538217947637579E-5	0.0	2.5007665418201865	0.0
84-85	0.0	2.538217947637579E-5	0.0	2.747671692676632	0.0
86-87	0.0	2.538217947637579E-5	0.0	3.01283932166633	0.0
88	0.0	2.538217947637579E-5	0.0	3.23127835824002	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	4975	0.0	53.54847	1
GTATCAA	9805	0.0	45.203487	1
TCAACGC	11640	0.0	37.307133	4
ATCAACG	11695	0.0	37.133095	3
CAACGCA	11810	0.0	36.730316	5
AACGCAG	12270	0.0	35.468216	6
TATCAAC	12505	0.0	34.97405	2
ACGCAGA	14215	0.0	30.317638	7
CGCAGAG	14355	0.0	29.825523	8
GCAGAGT	16275	0.0	25.960083	9
GTACATG	9870	0.0	23.981607	1
TACATGG	9845	0.0	22.881992	2
AGAGTAC	15065	0.0	21.681185	10-11
GTGGTAT	2575	0.0	21.6071	1
ACATGGG	10240	0.0	21.15877	3
GAGTACT	11570	0.0	20.736359	12-13
CAGAGTA	16150	0.0	20.501038	10-11
AGTACTT	12230	0.0	19.482811	12-13
GTACTTT	12705	0.0	18.625061	14-15
CATGGGG	7465	0.0	18.572275	4
>>END_MODULE