FastQCFastQC Report
Wed 25 May 2016
SRR1033079_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033079_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2793776
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA22600.033.9081041
TCAACGC29350.032.3540534
GTATCAA32200.032.2671621
ATCAACG30200.031.4434263
CAACGCA30850.030.7809245
AACGCAG32200.029.630046
TATCAAC35500.027.4111612
GTACATG69800.025.1233631
ACGCAGA38850.023.9537777
TACATGG69650.023.5553592
ACATGGG69150.022.9778823
CGCAGAG40000.022.5779028
GTATTAG12150.018.573311
GTACCGT2803.9444785E-818.4640396
CATGGGG53250.017.9209844
GCAGAGT50800.017.3183279
CTGTCGC5750.017.1822289
CCGGTCG1650.001561668617.1078479
CCGCGCG1650.001561668617.1078479
TACACCG3151.5101068E-716.4160085
Produced by FastQC (version 0.11.5)