FastQCFastQC Report
Wed 25 May 2016
SRR1033079_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033079_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2793776
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42210.1510858422436158No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35470.1269607871210863No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA37750.051.3082581
GTATCAA65500.042.3056531
TCAACGC76000.035.869464
ATCAACG76350.035.7685133
CAACGCA77700.035.2031335
AACGCAG80450.033.940776
TATCAAC82450.033.2536162
ACGCAGA93200.028.94467
CGCAGAG93500.028.7009378
GTACATG68100.027.0577721
GTGGTAT17450.027.0064161
TACATGG69300.025.2201252
GCAGAGT108600.024.4506389
ACATGGG69000.024.1831573
GAGTACT75850.021.28367212-13
AGAGTAC99750.020.4007710-11
CAGAGTA110800.019.9992410-11
TGGTATC20750.019.978882
AGTACTT82000.018.62708912-13
GTACTTT87750.018.50462314-15
Produced by FastQC (version 0.11.5)