##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1033079_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2793776 Sequences flagged as poor quality 0 Sequence length 100 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.7258019254228 34.0 31.0 34.0 31.0 34.0 2 32.724895983070944 34.0 31.0 34.0 31.0 34.0 3 32.82670836888856 34.0 31.0 34.0 31.0 34.0 4 36.31318509429532 37.0 37.0 37.0 35.0 37.0 5 36.29441336742817 37.0 37.0 37.0 35.0 37.0 6 36.36470318307552 37.0 37.0 37.0 35.0 37.0 7 36.31571428775965 37.0 37.0 37.0 35.0 37.0 8 36.320536435276125 37.0 37.0 37.0 35.0 37.0 9 38.12249335666138 39.0 39.0 39.0 37.0 39.0 10-11 38.085677591904286 39.0 39.0 39.0 36.0 39.0 12-13 38.06295440292994 39.0 39.0 39.0 35.0 39.0 14-15 39.568506387054654 41.0 39.5 41.0 37.0 41.0 16-17 39.570679789646704 41.0 40.0 41.0 37.0 41.0 18-19 39.58721994891502 41.0 40.0 41.0 37.0 41.0 20-21 39.57888320323462 41.0 40.0 41.0 37.0 41.0 22-23 39.513426452228096 41.0 39.0 41.0 37.0 41.0 24-25 39.466231007783016 41.0 39.0 41.0 36.0 41.0 26-27 39.363984800499395 41.0 39.0 41.0 36.0 41.0 28-29 39.31306446901971 41.0 39.0 41.0 36.0 41.0 30-31 39.312689886376006 41.0 39.0 41.0 36.0 41.0 32-33 39.27978102038245 41.0 39.0 41.0 35.5 41.0 34-35 39.206621790723375 41.0 39.0 41.0 35.0 41.0 36-37 39.1254445596211 41.0 39.0 41.0 35.0 41.0 38-39 39.03375127426107 41.0 39.0 41.0 35.0 41.0 40-41 38.93507997777918 41.0 39.0 41.0 35.0 41.0 42-43 38.82122117163294 41.0 39.0 41.0 35.0 41.0 44-45 38.66451623179525 41.0 39.0 41.0 35.0 41.0 46-47 38.48158191637411 41.0 38.5 41.0 35.0 41.0 48-49 38.28990370022507 40.5 38.0 41.0 34.0 41.0 50-51 38.160361281648925 40.0 38.0 41.0 34.0 41.0 52-53 38.03165769195526 40.0 38.0 41.0 34.0 41.0 54-55 37.89015565313755 40.0 38.0 41.0 34.0 41.0 56-57 37.749621301063506 40.0 37.0 41.0 33.5 41.0 58-59 37.57474650795196 40.0 37.0 41.0 33.0 41.0 60-61 37.389705545469646 40.0 36.5 41.0 33.0 41.0 62-63 37.16355301928286 39.5 36.0 41.0 33.0 41.0 64-65 36.90286640732829 39.0 35.5 41.0 32.5 41.0 66-67 36.62880202278207 39.0 35.0 41.0 32.0 41.0 68-69 36.324744718259446 38.5 35.0 40.5 32.0 41.0 70-71 35.999671412453964 37.0 35.0 40.0 31.5 41.0 72-73 35.65315490576195 37.0 35.0 39.5 31.0 41.0 74-75 35.302772305295775 36.5 35.0 39.0 31.0 41.0 76-77 34.945305207002995 36.0 35.0 39.0 31.0 40.5 78-79 34.5774115390783 35.5 35.0 37.5 31.0 39.5 80-81 34.23668665633895 35.0 34.0 37.0 30.0 39.0 82-83 33.930472593364684 35.0 34.0 37.0 30.0 39.0 84-85 33.697158075665335 35.0 34.0 36.0 30.0 38.0 86-87 33.48019615745858 35.0 34.0 36.0 30.0 37.0 88-89 33.26649147963187 35.0 34.0 36.0 30.0 37.0 90-91 33.08016444410718 35.0 34.0 35.0 30.0 36.5 92-93 32.903805995899454 35.0 34.0 35.0 30.0 36.0 94-95 32.7503901529686 35.0 34.0 35.0 29.5 36.0 96-97 32.60820069325529 35.0 34.0 35.0 29.0 36.0 98-99 32.48352534347779 35.0 34.0 35.0 29.0 36.0 100 32.383224710928864 35.0 34.0 35.0 29.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.15283369243115175 1101 2 0.09010209893982335 1101 3 0.124397981881323 1101 4 0.11153899634346232 1101 5 0.08877018297548744 1101 6 0.12716147912183118 1101 7 0.17370544567791768 1101 8 0.14694801429157422 1101 9 0.21375654030675406 1101 10-11 0.25915902646067934 1101 12-13 0.2605181466489981 1101 14-15 0.31603539744493503 1101 16-17 0.33117262870784003 1101 18-19 0.30789068882117476 1101 20-21 0.2995694755186591 1101 22-23 0.29212061766526176 1101 24-25 0.2982298319319767 1101 26-27 0.32048488918492524 1101 28-29 0.36088049335020855 1101 30-31 0.3675797216455905 1101 32-33 0.32776053303954455 1101 34-35 0.35505754380375976 1101 36-37 0.3149623956532963 1101 38-39 0.24456228552418224 1101 40-41 0.33346147429896433 1101 42-43 0.347218276537987 1101 44-45 0.3274045793456395 1101 46-47 0.3649910841513986 1101 48-49 0.3687026396842228 1101 50-51 0.45484574768080677 1101 52-53 0.3977430609073238 1101 54-55 0.3706781552501184 1101 56-57 0.29381224628288294 1101 58-59 0.3121646626486907 1101 60-61 0.2689559096001801 1101 62-63 0.25948287228492006 1101 64-65 0.33783256085039426 1101 66-67 0.3637431319080022 1101 68-69 0.30070423891586984 1101 70-71 0.3547720890840651 1101 72-73 0.3182945095830405 1101 74-75 0.32606897359045917 1101 76-77 0.30688736907615066 1101 78-79 0.31382084461245086 1101 80-81 0.2879496976425884 1101 82-83 0.28947873709817173 1101 84-85 0.2687095034935254 1101 86-87 0.29119804492129475 1101 88-89 0.31007660586467267 1101 90-91 0.2948999240578445 1101 92-93 0.2625349611122658 1101 94-95 0.29619405341954774 1101 96-97 0.3312674731480314 1101 98-99 0.3672464033917535 1101 100 0.42340326636372794 1102 1 0.07496546181680941 1102 2 -0.02869236472424319 1102 3 0.03877096187621021 1102 4 0.03100706743422421 1102 5 0.06263506553596443 1102 6 0.1147657150608552 1102 7 0.12090365586222163 1102 8 0.11086244251102784 1102 9 0.1833148144432286 1102 10-11 0.1289633945461972 1102 12-13 0.1505984308567463 1102 14-15 0.24289474421187407 1102 16-17 0.23811415238351685 1102 18-19 0.2635671217392712 1102 20-21 0.2335916106994702 1102 22-23 0.2401524156127266 1102 24-25 0.2721537008183361 1102 26-27 0.24396863372395217 1102 28-29 0.29428314721387494 1102 30-31 0.2331110603347213 1102 32-33 0.28411903772583 1102 34-35 0.28296412088257483 1102 36-37 0.28677617414533074 1102 38-39 0.2730982142273106 1102 40-41 0.2900031113654151 1102 42-43 0.2787052843447384 1102 44-45 0.29882044100775573 1102 46-47 0.2886665253284306 1102 48-49 0.36097817869363524 1102 50-51 0.29095921753126675 1102 52-53 0.23903448104826452 1102 54-55 0.17033771886407578 1102 56-57 0.19658129922298428 1102 58-59 0.2302449314407795 1102 60-61 0.2071826671678707 1102 62-63 0.16715182261094697 1102 64-65 0.13902633988769253 1102 66-67 0.10569722577312746 1102 68-69 0.13782586465180913 1102 70-71 0.12438623786606229 1102 72-73 0.15797024679792315 1102 74-75 0.17312103471470408 1102 76-77 0.13165226949310238 1102 78-79 0.21055384431084434 1102 80-81 0.1530617445416098 1102 82-83 0.20856912441816178 1102 84-85 0.27334526489386946 1102 86-87 0.21895478317231465 1102 88-89 0.22110902571999702 1102 90-91 0.2482639359901384 1102 92-93 0.2737564940544672 1102 94-95 0.28661646545428 1102 96-97 0.2415157281448117 1102 98-99 0.2081312710761125 1102 100 0.17347153063560228 1103 1 0.15038808408903748 1103 2 0.03801777142839313 1103 3 0.057836049260501454 1103 4 0.08612445547374392 1103 5 0.13250899585568732 1103 6 0.11712160595244114 1103 7 0.1223362287246772 1103 8 0.09830654295822683 1103 9 0.14584587428976192 1103 10-11 0.18107948830628118 1103 12-13 0.18412815311777564 1103 14-15 0.19785071501541296 1103 16-17 0.2157354359263124 1103 18-19 0.21741797074245994 1103 20-21 0.2499481283033802 1103 22-23 0.20350955911187896 1103 24-25 0.21311507098960192 1103 26-27 0.2268407778726953 1103 28-29 0.281972682752226 1103 30-31 0.249507922033942 1103 32-33 0.22336164730039343 1103 34-35 0.20956135893602124 1103 36-37 0.22216260316790937 1103 38-39 0.24366306015984662 1103 40-41 0.2183713019970881 1103 42-43 0.23554333272823413 1103 44-45 0.2476864961600782 1103 46-47 0.30402138586246963 1103 48-49 0.31099133393281164 1103 50-51 0.2917259596158601 1103 52-53 0.27898963884167927 1103 54-55 0.28864897427426683 1103 56-57 0.3415613294065025 1103 58-59 0.2961656511059019 1103 60-61 0.3517010383368202 1103 62-63 0.37808413277716113 1103 64-65 0.32417918170533255 1103 66-67 0.3235394070261748 1103 68-69 0.342725251584973 1103 70-71 0.38360011427381835 1103 72-73 0.3780595430906786 1103 74-75 0.3021176535731058 1103 76-77 0.27198843896526625 1103 78-79 0.28574839477344227 1103 80-81 0.23663050354497983 1103 82-83 0.2723024791594 1103 84-85 0.25507970555516124 1103 86-87 0.20342802615122224 1103 88-89 0.3200847351019718 1103 90-91 0.2834891316232486 1103 92-93 0.30546971065753326 1103 94-95 0.30750193970065 1103 96-97 0.35754258852850995 1103 98-99 0.3915877202891238 1103 100 0.3819918767246122 1104 1 0.09623628791091932 1104 2 0.10800200409297389 1104 3 0.10109704171554057 1104 4 0.06474236436763192 1104 5 0.07452911384145011 1104 6 0.08660637490413592 1104 7 0.10534823203474986 1104 8 0.13326952314851326 1104 9 0.10874886680567641 1104 10-11 0.1514081973176502 1104 12-13 0.1647093697234041 1104 14-15 0.24640752646008934 1104 16-17 0.22768557904460351 1104 18-19 0.18799801149663864 1104 20-21 0.2049583155473229 1104 22-23 0.20909465836856356 1104 24-25 0.22734319865689656 1104 26-27 0.21388426720665166 1104 28-29 0.21639246249860378 1104 30-31 0.18642316681786042 1104 32-33 0.17361187993428473 1104 34-35 0.1541235402757266 1104 36-37 0.2276194188529388 1104 38-39 0.21081820595372136 1104 40-41 0.16626882368205287 1104 42-43 0.22888003362727005 1104 44-45 0.2755284536613729 1104 46-47 0.261887402793171 1104 48-49 0.1558322076736829 1104 50-51 0.1754864430989116 1104 52-53 0.13844508434759462 1104 54-55 0.14014248120697914 1104 56-57 0.19289653863911838 1104 58-59 0.20581073517537618 1104 60-61 0.0891377355157914 1104 62-63 0.11853407935657145 1104 64-65 0.1220026468615032 1104 66-67 0.15563484454046517 1104 68-69 0.16413435865847958 1104 70-71 0.17801520362161938 1104 72-73 0.188266941693648 1104 74-75 0.11284612085855628 1104 76-77 0.0855024319876776 1104 78-79 0.05957645849731108 1104 80-81 0.11376173428944014 1104 82-83 0.13517026767159024 1104 84-85 0.16831520128450705 1104 86-87 0.21760936039401457 1104 88-89 0.19555514071608116 1104 90-91 0.1587940530558214 1104 92-93 0.18538181706271928 1104 94-95 0.1741975539048326 1104 96-97 0.17592917856711665 1104 98-99 0.2520358432582128 1104 100 0.1714491718552651 1105 1 0.0549417476597327 1105 2 0.1135079675068269 1105 3 0.13683501726875846 1105 4 0.06534272172761746 1105 5 0.05464521876589856 1105 6 0.09898435105048264 1105 7 0.11209371066482277 1105 8 0.1372513313999164 1105 9 0.11302853682845893 1105 10-11 0.1222882086365189 1105 12-13 0.14197129037268041 1105 14-15 0.17398590593388974 1105 16-17 0.1651613905186764 1105 18-19 0.17235863121717898 1105 20-21 0.20584983108976473 1105 22-23 0.16840893991683004 1105 24-25 0.18545769224863307 1105 26-27 0.19459748581903114 1105 28-29 0.20522474673570912 1105 30-31 0.221534495821345 1105 32-33 0.20828166794022707 1105 34-35 0.2270278192043449 1105 36-37 0.21735094166120206 1105 38-39 0.2077432975179505 1105 40-41 0.20816103367923944 1105 42-43 0.22181139258375993 1105 44-45 0.315694752094025 1105 46-47 0.2662569379780564 1105 48-49 0.21211362495448327 1105 50-51 0.313197837840832 1105 52-53 0.3191986171698531 1105 54-55 0.27437523846415957 1105 56-57 0.20780874942714433 1105 58-59 0.274158427163691 1105 60-61 0.23084190520988557 1105 62-63 0.22496235775136597 1105 64-65 0.22295369150926092 1105 66-67 0.2776931737960453 1105 68-69 0.29749821340128335 1105 70-71 0.2949842732005479 1105 72-73 0.2671268805519773 1105 74-75 0.2385082624482422 1105 76-77 0.23541723153492455 1105 78-79 0.2564720789839683 1105 80-81 0.31060496174931984 1105 82-83 0.28224184964443566 1105 84-85 0.31578661989984624 1105 86-87 0.31923684968062815 1105 88-89 0.28199133984912805 1105 90-91 0.23365683028166018 1105 92-93 0.20129014409405954 1105 94-95 0.22082753309589975 1105 96-97 0.2508281922436737 1105 98-99 0.2545392166771592 1105 100 0.29213119561006096 1106 1 0.013820128363548179 1106 2 0.031129691964530082 1106 3 0.051351130881435836 1106 4 0.06078719644582975 1106 5 0.06367524715950879 1106 6 0.034749066492970826 1106 7 0.06412165494833744 1106 8 0.05379001171846198 1106 9 0.07698633837911473 1106 10-11 0.09750444700900118 1106 12-13 0.11625117297418797 1106 14-15 0.14239323473571375 1106 16-17 0.16039739447657553 1106 18-19 0.12593424234900397 1106 20-21 0.10821411297999362 1106 22-23 0.1304395371386491 1106 24-25 0.09120620325712991 1106 26-27 0.10560520802053475 1106 28-29 0.06879625895835062 1106 30-31 0.042596973713528996 1106 32-33 0.04873025351781024 1106 34-35 0.06472224851190589 1106 36-37 0.04967173687348492 1106 38-39 0.11567325277650298 1106 40-41 0.07906493278154159 1106 42-43 0.07813572265708757 1106 44-45 0.07619680274363816 1106 46-47 0.10671108038587107 1106 48-49 0.1438022560383203 1106 50-51 0.10717082806080214 1106 52-53 0.11923482619270942 1106 54-55 0.1792576607163312 1106 56-57 0.16739882265780182 1106 58-59 0.15033360713328392 1106 60-61 0.1665393411808651 1106 62-63 0.1482685402926407 1106 64-65 0.16512716499421742 1106 66-67 0.1465646473001172 1106 68-69 0.23811469890333115 1106 70-71 0.20149514546753977 1106 72-73 0.2085334517128672 1106 74-75 0.20341646122262347 1106 76-77 0.17890187795322277 1106 78-79 0.23115390563955174 1106 80-81 0.23101675274557465 1106 82-83 0.19407532876987688 1106 84-85 0.2243039858906144 1106 86-87 0.2641265376377291 1106 88-89 0.24321386640241371 1106 90-91 0.15579512245859917 1106 92-93 0.21701824420782145 1106 94-95 0.22129370932749026 1106 96-97 0.1295151192328987 1106 98-99 0.12973711153475875 1106 100 0.06365512941566465 1107 1 -0.004138858266848899 1107 2 0.038243519622412236 1107 3 0.04608332185375019 1107 4 0.03468150987445284 1107 5 -0.013416479597651687 1107 6 0.02263446899239341 1107 7 0.053529100610276714 1107 8 0.03225446423199685 1107 9 0.03766181119083711 1107 10-11 0.055017550373214874 1107 12-13 0.07018290396548821 1107 14-15 0.05374504643219069 1107 16-17 0.02997922554742871 1107 18-19 0.05018693359075144 1107 20-21 0.007655816641879198 1107 22-23 0.0644371538339712 1107 24-25 0.09911914367130947 1107 26-27 0.09641360700435087 1107 28-29 0.045676519550099215 1107 30-31 -0.03446027050623002 1107 32-33 0.042670949968837135 1107 34-35 -0.002229607450523474 1107 36-37 0.014211659632600515 1107 38-39 0.04470979464311853 1107 40-41 -0.01209687211466104 1107 42-43 0.020598802284482076 1107 44-45 6.419546592653091E-4 1107 46-47 -0.05356375345641595 1107 48-49 -0.05673174213972487 1107 50-51 -0.00423222629008535 1107 52-53 -0.008459222281715029 1107 54-55 -0.05560089672675872 1107 56-57 0.0017250659537495494 1107 58-59 0.001029833621011278 1107 60-61 0.08912363895601061 1107 62-63 0.022005517120376794 1107 64-65 0.03988618819214196 1107 66-67 0.08464483603988526 1107 68-69 0.04497720499792024 1107 70-71 0.040181825905840185 1107 72-73 0.07015743487897197 1107 74-75 0.02397053946430816 1107 76-77 -0.030820513563114105 1107 78-79 0.07605826872302401 1107 80-81 0.006970876536286141 1107 82-83 0.02618626951764469 1107 84-85 0.029932738873753806 1107 86-87 0.049147563966343455 1107 88-89 0.04114980586711425 1107 90-91 0.12237633123993419 1107 92-93 0.08630737467050409 1107 94-95 0.033750004716132764 1107 96-97 0.11948190372827128 1107 98-99 0.14645512777843805 1107 100 0.0889661137878619 1108 1 -0.09603046516095759 1108 2 0.016880110380085966 1108 3 -0.001218639176556735 1108 4 -0.015586168815943324 1108 5 -0.013678750637744486 1108 6 -0.003062739362206912 1108 7 -0.022502624992569054 1108 8 -0.02472124243974605 1108 9 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50-51 -0.23943420978791607 2214 52-53 -0.29628326762559 2214 54-55 -0.26244756159012184 2214 56-57 -0.2780251775692619 2214 58-59 -0.29822097442393414 2214 60-61 -0.28222801133804865 2214 62-63 -0.2732905766403846 2214 64-65 -0.24819682752188044 2214 66-67 -0.2676847351530043 2214 68-69 -0.29989136526755544 2214 70-71 -0.2511564927944292 2214 72-73 -0.2567684820913527 2214 74-75 -0.23578527849755204 2214 76-77 -0.2553682695167083 2214 78-79 -0.2727068849891552 2214 80-81 -0.29851836751318217 2214 82-83 -0.2712433766260034 2214 84-85 -0.2434999667071125 2214 86-87 -0.28586059587198775 2214 88-89 -0.2397257033695439 2214 90-91 -0.3048523969386423 2214 92-93 -0.3167012393105182 2214 94-95 -0.3260387739805637 2214 96-97 -0.25274438730718174 2214 98-99 -0.24490613239673564 2214 100 -0.19834814491507302 2215 1 -0.1895736623824007 2215 2 -0.14736441468704697 2215 3 -0.1988835360805581 2215 4 -0.11581809439939406 2215 5 -0.12132605617066616 2215 6 -0.16210781434752874 2215 7 -0.2060796251514958 2215 8 -0.17232359275256925 2215 9 -0.24743415240235578 2215 10-11 -0.22438366648472652 2215 12-13 -0.21132060810415965 2215 14-15 -0.34941334296235027 2215 16-17 -0.2998152441795199 2215 18-19 -0.34728907551094323 2215 20-21 -0.35281361351245977 2215 22-23 -0.34748286001644146 2215 24-25 -0.3744723185368457 2215 26-27 -0.3957460685002019 2215 28-29 -0.3953911990885999 2215 30-31 -0.4015333809138326 2215 32-33 -0.3370777639211582 2215 34-35 -0.3075014218549583 2215 36-37 -0.3370324044750319 2215 38-39 -0.3323677676837491 2215 40-41 -0.3393067553754392 2215 42-43 -0.31207086530555017 2215 44-45 -0.30106832432768016 2215 46-47 -0.34719256232068574 2215 48-49 -0.2535003907348923 2215 50-51 -0.18236464615553416 2215 52-53 -0.2375784842524311 2215 54-55 -0.21995709973428745 2215 56-57 -0.17566536736639193 2215 58-59 -0.22739072435287255 2215 60-61 -0.2106636582343313 2215 62-63 -0.1817316217795053 2215 64-65 -0.261218135116124 2215 66-67 -0.2476971208259613 2215 68-69 -0.22584788404046208 2215 70-71 -0.25709508347286913 2215 72-73 -0.18810334865882083 2215 74-75 -0.10442404898048352 2215 76-77 -0.13956482499959577 2215 78-79 -0.16377033238500616 2215 80-81 -0.19498549465485127 2215 82-83 -0.22409815865805882 2215 84-85 -0.22751804268540354 2215 86-87 -0.2177264467182667 2215 88-89 -0.13420016445342497 2215 90-91 -0.10681712938531263 2215 92-93 -0.17637441203309834 2215 94-95 -0.1324611646546643 2215 96-97 -0.18912885013006786 2215 98-99 -0.1844275888816327 2215 100 -0.2012619234103994 2216 1 -0.18489889920994784 2216 2 -0.13782233953560308 2216 3 -0.17583127111154795 2216 4 -0.16911680163156717 2216 5 -0.2267336350264202 2216 6 -0.19904698366990203 2216 7 -0.16285269876711794 2216 8 -0.17564744547417632 2216 9 -0.20410468301896856 2216 10-11 -0.25037311473527524 2216 12-13 -0.26317970893379794 2216 14-15 -0.3484827278218674 2216 16-17 -0.3406039300790198 2216 18-19 -0.3669697528695792 2216 20-21 -0.3406112211768004 2216 22-23 -0.3828732932336649 2216 24-25 -0.3867035838373667 2216 26-27 -0.3764766110222766 2216 28-29 -0.40362281962201507 2216 30-31 -0.4005365432051917 2216 32-33 -0.36639757674799966 2216 34-35 -0.40789904543218825 2216 36-37 -0.4367952344941486 2216 38-39 -0.48510354379092036 2216 40-41 -0.49740332228822837 2216 42-43 -0.4151121147613068 2216 44-45 -0.4217886978775951 2216 46-47 -0.3538982382403475 2216 48-49 -0.38804951103946195 2216 50-51 -0.4296939249736198 2216 52-53 -0.45934072461187725 2216 54-55 -0.45493052725049665 2216 56-57 -0.4722267937290283 2216 58-59 -0.4580904852630141 2216 60-61 -0.4330104685370344 2216 62-63 -0.44774112878794625 2216 64-65 -0.5082241976528366 2216 66-67 -0.47681001011417834 2216 68-69 -0.49028786566056937 2216 70-71 -0.4506295752327176 2216 72-73 -0.5298945083894537 2216 74-75 -0.4952340930148509 2216 76-77 -0.46455833747081243 2216 78-79 -0.4933659671669588 2216 80-81 -0.49611474381779885 2216 82-83 -0.45389907778763217 2216 84-85 -0.46674127871941096 2216 86-87 -0.5274448696308909 2216 88-89 -0.6199954516520378 2216 90-91 -0.576122660292711 2216 92-93 -0.5315656027062658 2216 94-95 -0.3803159950549073 2216 96-97 -0.5418137439520265 2216 98-99 -0.6686166373043108 2216 100 -0.6689573233988249 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 25.0 3 4.0 4 6.0 5 27.0 6 54.0 7 137.0 8 363.0 9 595.0 10 1072.0 11 1733.0 12 2675.0 13 4211.0 14 5160.0 15 7017.0 16 8639.0 17 11961.0 18 13961.0 19 7537.0 20 5073.0 21 4641.0 22 4938.0 23 5398.0 24 6074.0 25 7566.0 26 9644.0 27 12478.0 28 16512.0 29 20998.0 30 27310.0 31 34918.0 32 45705.0 33 59736.0 34 83446.0 35 124404.0 36 227911.0 37 525069.0 38 999479.0 39 505458.0 40 1841.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.52217591261343 20.092087645838564 13.822743815077008 23.562992626471008 2 15.497155339694348 20.307064000551133 40.29862474596121 23.897155913793302 3 18.183676259925015 25.568905443609154 30.28999555427967 25.957422742186164 4 12.230487250058077 17.047112090457848 39.25082838201252 31.471572277471555 5 12.532133998145875 37.79670481106175 36.03614470768889 13.635016483103476 6 30.558914214249256 38.56163741233185 17.715892552511406 13.163555820907488 7 26.750426662695936 32.70509160362177 22.864467301601845 17.680014432080455 8 24.973745079215767 36.295933583462016 20.405138304551354 18.325183032770866 9 24.785916981175298 16.644892074382483 21.553696502511297 37.01549444193092 10-11 23.686169780571625 26.876763528994445 29.104407359403584 20.332659331030342 12-13 24.71164474174021 24.087704239709986 29.34986555829816 21.850785460251647 14-15 21.718221311941903 25.97942133327511 26.849089419411136 25.453267935371848 16-17 20.942417465989088 29.58934558916304 27.34028987686005 22.12794706798782 18-19 20.785577463256143 28.121229888038247 30.031804733860184 21.061387914845426 20-21 21.857071192728263 27.39178577519919 29.76404152731671 20.987101504755838 22-23 21.331880577921282 27.24141899221178 29.806768377442467 21.619932052424474 24-25 21.29646764468482 27.400657540326694 29.81889900474095 21.483975810247536 26-27 21.137601576363142 27.373721374950893 29.844717134269168 21.643959914416797 28-29 21.163873632199984 27.373199361220546 29.930214257424254 21.532712749155216 30-31 21.486312063896364 27.82592650658214 29.461438183618288 21.22632324590321 32-33 21.0874458081106 27.474124625596325 29.95454896885076 21.483880597442315 34-35 21.166890258918393 27.342492741007153 29.946209001723833 21.54440799835062 36-37 21.583494882911154 27.673854310438635 29.275468040386915 21.467182766263292 38-39 21.511244996019723 27.78603223737336 29.38635738870976 21.316365377897153 40-41 21.347954657025703 27.546524440868236 29.57401521633733 21.531505685768728 42-43 21.31248174513633 27.51577077045547 29.39034840302157 21.781399081386628 44-45 21.54141921184805 27.711598925611792 29.17834142751602 21.56864043502414 46-47 21.58319063518335 27.906925966863483 28.90965489001266 21.600228507940507 48-49 21.593409779452614 27.853736305272864 28.709227225088913 21.843626690185612 50-51 21.667986266615504 27.81296710974681 28.61397978936035 21.90506683427734 52-53 21.721694939035917 27.815544266970583 28.510678737307497 21.952082056686002 54-55 21.779448316543633 28.20839967126928 28.304291396303782 21.707860615883305 56-57 21.585320369277994 27.900787321531862 28.45949353133537 22.054398777854775 58-59 21.413366712291896 27.60819049200795 28.850970872396353 22.1274719233038 60-61 21.67297950873656 27.946692934580298 28.50147971777265 21.87884783891049 62-63 21.560139395570726 27.93624113028389 28.50373473034345 21.999884743801935 64-65 21.569571075132725 27.660431616564825 28.635814038061746 22.134183270240708 66-67 21.422211627240628 27.811772993212948 28.485878697125088 22.280136682421332 68-69 21.82267655843599 27.8719207608499 28.45697239626519 21.84843028444892 70-71 21.76320569838301 27.70063802561051 28.49916331844905 22.036992957557427 72-73 21.636153493170152 27.861155873469716 28.524633058029913 21.97805757533022 74-75 21.633859754432272 27.981409356743985 28.33695030838247 22.047780580441277 76-77 21.654348809639714 27.919095875975742 28.25788466935073 22.168670645033817 78-79 21.80040561591194 27.997717068225942 28.342125496102767 21.859751819759353 80-81 21.81517057917313 27.736314579264764 28.384541208744007 22.063973632818094 82-83 21.553198168353813 27.852891307125745 28.536143441964285 22.057767082556158 84-85 21.546645113996256 27.7949806999559 28.377525614079296 22.280848571968548 86-87 21.67314956433281 27.93538888974397 28.239155031514862 22.152306514408355 88-89 21.449461547313874 27.76134297908562 28.39448194052367 22.39471353307684 90-91 21.36515202848119 27.683897020567862 28.473565645029975 22.477385305920976 92-93 21.55914832934587 27.886597827440905 28.29253879007329 22.26171505313993 94-95 21.318811436065687 27.643339186858707 28.408061829263666 22.629787547811944 96-97 21.35644023475818 27.652989143517082 28.41362840192378 22.576942219800962 98-99 21.227788094375633 27.74490903337853 28.338121364234446 22.68918150801139 100 21.400641998499523 27.56677700717595 28.260569208125492 22.772011786199037 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 26.0 1 22.0 2 67.5 3 126.5 4 87.0 5 58.0 6 172.0 7 401.5 8 713.0 9 892.0 10 777.5 11 668.5 12 757.0 13 910.5 14 1105.0 15 1291.0 16 1507.5 17 1804.5 18 2121.0 19 2535.0 20 3045.5 21 3794.5 22 5003.0 23 6980.0 24 9488.0 25 12532.5 26 16753.0 27 22344.5 28 29252.5 29 37080.0 30 45254.0 31 53809.0 32 62489.5 33 71538.5 34 79919.0 35 86420.5 36 91757.5 37 96965.5 38 101213.5 39 102675.5 40 103012.0 41 103742.5 42 104119.5 43 103467.5 44 100899.0 45 98719.0 46 96100.0 47 92253.5 48 90398.0 49 88479.0 50 85050.0 51 81339.5 52 76093.0 53 72416.5 54 71173.5 55 67644.0 56 61514.5 57 56158.0 58 50483.5 59 43096.5 60 36740.0 61 31675.0 62 27005.0 63 22200.0 64 17668.5 65 13934.5 66 10816.0 67 8092.0 68 6363.0 69 5267.0 70 4016.0 71 2821.5 72 2135.0 73 1583.5 74 1132.0 75 748.5 76 430.0 77 287.5 78 185.5 79 85.5 80 45.0 81 21.0 82 9.0 83 2.0 84 1.0 85 2.5 86 3.0 87 1.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04363270355246805 2 0.24339818224510482 3 0.002791920325752673 4 0.0027561264754225106 5 2.1476310198097486E-4 6 7.158770066032494E-5 7 0.0 8 5.011139046222746E-4 9 0.0 10-11 6.084954556127621E-4 12-13 0.0 14-15 3.221446529714623E-4 16-17 1.2527847615556866E-4 18-19 3.400415781365435E-4 20-21 1.7896925165081236E-5 22-23 1.6107232648573114E-4 24-25 0.0011096093602350368 26-27 1.2527847615556866E-4 28-29 3.75835428466706E-4 30-31 7.158770066032494E-5 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 8.948462582540619E-5 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 1.7896925165081236E-5 68-69 1.968661768158936E-4 70-71 3.579385033016247E-5 72-73 3.5793850330162474E-4 74-75 1.7896925165081237E-4 76-77 0.0 78-79 0.0 80-81 0.0 82-83 1.7896925165081237E-4 84-85 0.0 86-87 5.369077549524371E-4 88-89 0.0027382295502574295 90-91 0.0025950541489367794 92-93 7.158770066032495E-4 94-95 6.442893059429246E-4 96-97 2.6845387747621857E-4 98-99 1.4317540132064989E-4 100 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2793776.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 65.5890151997504 #Duplication Level Percentage of deduplicated Percentage of total 1 77.52536668875922 50.84812454115253 2 13.971653488988148 18.32773986109779 3 4.221891084317804 8.307290355030334 4 1.7017562261911927 4.464660599436961 5 0.8174690909491846 2.680849631579611 6 0.4771641700402747 1.8778036800928721 7 0.29121484220724475 1.3370346298346725 8 0.19377044704242383 1.0167370237062374 9 0.13817722236880015 0.8156617144385871 >10 0.6162750130281939 6.937880662980675 >50 0.03191236678907467 1.4267960503826072 >100 0.01274875159375041 1.43466709287791 >500 3.8220491586786416E-4 0.14550441158089206 >1k 2.1840280883835496E-4 0.3792497458083126 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4221 0.1510858422436158 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3547 0.1269607871210863 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 7.158770066032496E-5 0.0 5 0.0 0.0 0.0 7.158770066032496E-5 0.0 6 0.0 0.0 0.0 7.158770066032496E-5 0.0 7 0.0 0.0 0.0 7.158770066032496E-5 0.0 8 0.0 0.0 0.0 1.7896925165081237E-4 0.0 9 0.0 0.0 0.0 2.505569523111373E-4 0.0 10-11 0.0 0.0 0.0 2.505569523111373E-4 0.0 12-13 0.0 0.0 0.0 2.505569523111373E-4 0.0 14-15 0.0 0.0 0.0 4.1162927879686846E-4 0.0 16-17 0.0 0.0 0.0 5.369077549524371E-4 0.0 18-19 0.0 1.789692516508124E-5 0.0 5.727016052825997E-4 0.0 20-21 0.0 3.579385033016248E-5 0.0 6.442893059429246E-4 0.0 22-23 1.789692516508124E-5 3.579385033016248E-5 0.0 7.695677820984933E-4 0.0 24-25 3.579385033016248E-5 3.579385033016248E-5 0.0 8.053616324286558E-4 0.0 26-27 3.579385033016248E-5 3.579385033016248E-5 0.0 9.127431834191431E-4 0.0 28-29 3.579385033016248E-5 3.579385033016248E-5 0.0 0.0013780632377112553 0.0 30-31 3.579385033016248E-5 3.579385033016248E-5 0.0 0.003024580352898729 0.0 32-33 5.3690775495243714E-5 3.579385033016248E-5 0.0 0.008357864052092937 0.0 34-35 7.158770066032496E-5 3.579385033016248E-5 0.0 0.015158695614823808 0.0 36-37 7.158770066032496E-5 1.0738155099048743E-4 0.0 0.028652977189295062 0.0 38-39 7.158770066032496E-5 1.7896925165081237E-4 0.0 0.050558813591354494 0.0 40-41 7.158770066032496E-5 1.7896925165081237E-4 0.0 0.07837063529789073 0.0 42-43 7.158770066032496E-5 2.863508026412998E-4 0.0 0.10689833401103024 0.0 44-45 7.158770066032496E-5 2.863508026412998E-4 0.0 0.13913069623334157 0.0 46-47 7.158770066032496E-5 2.863508026412998E-4 0.0 0.17911242705213304 0.0 48-49 7.158770066032496E-5 2.863508026412998E-4 0.0 0.2266287633654237 0.0 50-51 7.158770066032496E-5 2.863508026412998E-4 0.0 0.28098172509177544 0.0 52-53 7.158770066032496E-5 2.863508026412998E-4 0.0 0.3360505638247304 0.0 54-55 7.158770066032496E-5 2.863508026412998E-4 0.0 0.3955578399986255 0.0 56-57 7.158770066032496E-5 2.863508026412998E-4 0.0 0.46564219894508363 0.0 58-59 7.158770066032496E-5 2.863508026412998E-4 0.0 0.5482901993574287 0.0 60-61 7.158770066032496E-5 3.221446529714623E-4 0.0 0.6385264960397683 0.0 62-63 7.158770066032496E-5 3.221446529714623E-4 0.0 0.7329685701359021 0.0 64-65 1.2527847615556868E-4 3.5793850330162474E-4 0.0 0.8313479677683536 0.0 66-67 1.431754013206499E-4 3.5793850330162474E-4 0.0 0.945333484144756 0.0 68-69 1.7896925165081237E-4 3.5793850330162474E-4 0.0 1.077949699618008 0.0 70-71 1.7896925165081237E-4 3.5793850330162474E-4 0.0 1.2229863811558264 0.0 72-73 1.7896925165081237E-4 3.5793850330162474E-4 0.0 1.3798708271529285 0.0 74-75 1.7896925165081237E-4 3.5793850330162474E-4 0.0 1.5403346581830468 0.0 76-77 2.505569523111373E-4 3.5793850330162474E-4 0.0 1.7076172177010611 0.0 78-79 2.863508026412998E-4 3.5793850330162474E-4 0.0 1.9075795625705139 0.0 80-81 3.221446529714623E-4 3.5793850330162474E-4 0.0 2.130825807079737 0.0 82-83 3.221446529714623E-4 3.5793850330162474E-4 0.0 2.367190497734965 0.0 84-85 3.579385033016248E-4 3.5793850330162474E-4 0.0 2.597989244663853 0.0 86-87 3.9373235363178726E-4 3.5793850330162474E-4 0.0 2.8421927885413862 0.0 88 3.9373235363178726E-4 3.5793850330162474E-4 0.0 3.0450902291379123 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3775 0.0 51.308258 1 GTATCAA 6550 0.0 42.305653 1 TCAACGC 7600 0.0 35.86946 4 ATCAACG 7635 0.0 35.768513 3 CAACGCA 7770 0.0 35.203133 5 AACGCAG 8045 0.0 33.94077 6 TATCAAC 8245 0.0 33.253616 2 ACGCAGA 9320 0.0 28.9446 7 CGCAGAG 9350 0.0 28.700937 8 GTACATG 6810 0.0 27.057772 1 GTGGTAT 1745 0.0 27.006416 1 TACATGG 6930 0.0 25.220125 2 GCAGAGT 10860 0.0 24.450638 9 ACATGGG 6900 0.0 24.183157 3 GAGTACT 7585 0.0 21.283672 12-13 AGAGTAC 9975 0.0 20.40077 10-11 CAGAGTA 11080 0.0 19.99924 10-11 TGGTATC 2075 0.0 19.97888 2 AGTACTT 8200 0.0 18.627089 12-13 GTACTTT 8775 0.0 18.504623 14-15 >>END_MODULE