FastQCFastQC Report
Wed 25 May 2016
SRR1033078_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033078_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2238668
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC22200.030.7020634
GTATCAA24250.029.4667951
CCGATCG804.7513237E-429.4076359
GGTATCA16150.029.4001751
CAACGCA23450.029.0667925
ATCAACG23300.029.0508673
AACGCAG25550.026.6729686
GTACATG52600.025.9187071
TATCAAC27700.025.1150742
TACATGG55300.023.205452
ACATGGG55050.022.3715723
ACGCAGA31750.020.8722677
CGCAGAG32900.020.0190838
GAGTACA40600.019.9160631
CATGGGG42850.019.1972834
AGTACAT40400.018.3835032
TCTGTCG5900.016.7447748
CTGTCGC5800.016.2249019
GTGTAAG10150.016.2107491
GTCTTAC7250.016.2107491
Produced by FastQC (version 0.11.5)