FastQCFastQC Report
Wed 25 May 2016
SRR1033078_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033078_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2238668
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44420.19842156139275677No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37190.16612557109852824No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA32800.054.902121
GTATCAA59350.041.7002031
TCAACGC73200.033.4499364
ATCAACG73400.033.359543
TATCAAC74300.033.099292
CAACGCA74900.032.6914565
AACGCAG76250.032.1742946
ACGCAGA88400.027.6458197
TCGCGTG350.00884160326.85533760-61
CGCAGAG91000.026.7532428
GTACATG58300.024.9855821
TACATGG59250.023.774962
GCAGAGT102100.023.6145529
ACATGGG60750.022.9767463
GTGGTAT15250.022.5658971
TCTGTCG6050.021.7515748
CTGTCGC6850.021.2695949
GAGTACT71300.020.82959212-13
TGGTATC15350.020.5637992
AGAGTAC91600.020.4465910-11
Produced by FastQC (version 0.11.5)