FastQCFastQC Report
Wed 25 May 2016
SRR1033077_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033077_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3455250
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA42400.033.039431
TCAACGC40700.032.6836134
ATCAACG41950.031.9338233
CAACGCA42650.031.1901875
GGTATCA27650.030.4326631
AACGCAG47150.028.1084066
GTACATG88450.026.3080961
TATCAAC53750.025.3601192
TACATGG88000.025.1576462
CGCAGAG54950.023.451958
ACATGGG92000.023.399993
ACGCAGA55450.023.3928227
CATGGGG63850.019.6556954
GAGTACA65450.019.2489591
GCAGAGT71300.017.4829799
TCTGTCG9750.017.3657688
AGTACAT68000.017.073392
AGAGTAC56000.016.2600610-11
GTACCGG2901.1487846E-616.210041
GTATTAG15950.015.9153121
Produced by FastQC (version 0.11.5)