FastQCFastQC Report
Wed 25 May 2016
SRR1033077_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033077_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3455250
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54280.1570942768251212No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44960.1301208306200709No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA45850.051.7131541
GTATCAA85650.041.992291
ATCAACG100450.035.044533
TCAACGC100450.034.950954
CAACGCA102550.034.1435745
TATCAAC108750.032.5363462
AACGCAG108200.032.4470636
ACGCAGA125050.027.9621987
CGCAGAG126700.027.4500718
GTACATG81500.026.663411
TACATGG80800.025.0735112
TATACCG1951.7595085E-824.1020375
GCAGAGT143150.024.098679
TAGACCG1452.5356023E-522.689165
ACATGGG86250.022.5595073
GAGTACT97600.021.38006212-13
AGAGTAC127450.020.7982910-11
GTGGTAT25350.020.6403681
CAGAGTA139250.020.14964910-11
CATGGGG60250.019.8916824
Produced by FastQC (version 0.11.5)