##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1033077_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3455250 Sequences flagged as poor quality 0 Sequence length 100 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.74456696331669 34.0 31.0 34.0 31.0 34.0 2 32.745602199551406 34.0 31.0 34.0 31.0 34.0 3 32.85762448448014 34.0 31.0 34.0 31.0 34.0 4 36.33367455321612 37.0 37.0 37.0 35.0 37.0 5 36.31539657043629 37.0 37.0 37.0 35.0 37.0 6 36.38211070110701 37.0 37.0 37.0 35.0 37.0 7 36.337137978438605 37.0 37.0 37.0 35.0 37.0 8 36.341183705954705 37.0 37.0 37.0 35.0 37.0 9 38.15116272339194 39.0 39.0 39.0 37.0 39.0 10-11 38.12141350119384 39.0 39.0 39.0 37.0 39.0 12-13 38.09337457492222 39.0 39.0 39.0 37.0 39.0 14-15 39.60547615946748 41.0 40.0 41.0 37.0 41.0 16-17 39.604993705231166 41.0 40.0 41.0 37.0 41.0 18-19 39.62346414875913 41.0 40.0 41.0 37.0 41.0 20-21 39.619845018450185 41.0 40.0 41.0 37.0 41.0 22-23 39.54732783445481 41.0 39.5 41.0 37.0 41.0 24-25 39.49537819260546 41.0 39.0 41.0 36.5 41.0 26-27 39.39099732291441 41.0 39.0 41.0 36.0 41.0 28-29 39.34098994284061 41.0 39.0 41.0 36.0 41.0 30-31 39.33418102886911 41.0 39.0 41.0 36.0 41.0 32-33 39.29269502930323 41.0 39.0 41.0 35.5 41.0 34-35 39.21198191158382 41.0 39.0 41.0 35.0 41.0 36-37 39.12409811156935 41.0 39.0 41.0 35.0 41.0 38-39 39.03130772013603 41.0 39.0 41.0 35.0 41.0 40-41 38.93147804066276 41.0 39.0 41.0 35.0 41.0 42-43 38.81594819477607 41.0 39.0 41.0 35.0 41.0 44-45 38.659031184429494 41.0 39.0 41.0 35.0 41.0 46-47 38.46596946675349 41.0 38.5 41.0 35.0 41.0 48-49 38.27110049924028 41.0 38.0 41.0 34.0 41.0 50-51 38.1411457926344 40.0 38.0 41.0 34.0 41.0 52-53 38.013818247594244 40.0 38.0 41.0 34.0 41.0 54-55 37.8756817885826 40.0 38.0 41.0 34.0 41.0 56-57 37.733256783156065 40.0 37.0 41.0 33.5 41.0 58-59 37.56180146154402 40.0 37.0 41.0 33.0 41.0 60-61 37.379496273786266 40.0 36.5 41.0 33.0 41.0 62-63 37.15568685333912 39.5 36.0 41.0 33.0 41.0 64-65 36.89674365096592 39.0 35.5 41.0 32.5 41.0 66-67 36.625574994573476 39.0 35.0 41.0 32.0 41.0 68-69 36.322843064901235 38.5 35.0 41.0 32.0 41.0 70-71 36.00120454381015 37.5 35.0 40.0 31.5 41.0 72-73 35.6564912813834 37.0 35.0 39.5 31.0 41.0 74-75 35.306455683380364 36.5 35.0 39.0 31.0 41.0 76-77 34.945469068808336 36.0 35.0 39.0 31.0 40.5 78-79 34.57815556037913 35.5 35.0 38.0 31.0 39.5 80-81 34.239138267853264 35.0 34.5 37.0 30.0 39.0 82-83 33.928367846031406 35.0 34.0 37.0 30.0 39.0 84-85 33.69042153245062 35.0 34.0 36.0 30.0 38.0 86-87 33.46947644888213 35.0 34.0 36.0 30.0 37.0 88-89 33.252440633818104 35.0 34.0 36.0 30.0 37.0 90-91 33.06348773605383 35.0 34.0 35.0 30.0 36.5 92-93 32.885252876058175 35.0 34.0 35.0 30.0 36.0 94-95 32.730510527458215 35.0 34.0 35.0 29.5 36.0 96-97 32.58951349395847 35.0 34.0 35.0 29.0 36.0 98-99 32.46640735113233 35.0 34.0 35.0 29.0 36.0 100 32.367817089935606 35.0 34.0 35.0 29.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.12206028344318298 1101 2 0.11951386079650916 1101 3 0.1516982987267852 1101 4 0.14464888496421224 1101 5 0.1356979604895585 1101 6 0.0907623476301751 1101 7 0.1485086209121036 1101 8 0.14297409857564958 1101 9 0.23715627976574893 1101 10-11 0.2249370773819237 1101 12-13 0.2285143516666608 1101 14-15 0.2696265852955264 1101 16-17 0.2938866508323059 1101 18-19 0.3046243197247236 1101 20-21 0.2693637260239541 1101 22-23 0.29152646589005826 1101 24-25 0.31834933854140246 1101 26-27 0.3515043986145372 1101 28-29 0.30841784998488464 1101 30-31 0.3385052313215482 1101 32-33 0.31742524016503637 1101 34-35 0.29570352020608226 1101 36-37 0.2831222291964508 1101 38-39 0.2859756292283322 1101 40-41 0.3238198143683917 1101 42-43 0.3060680471384529 1101 44-45 0.3431273336979217 1101 46-47 0.3260991924582086 1101 48-49 0.28125015626372374 1101 50-51 0.3639630344407223 1101 52-53 0.3596438115302547 1101 54-55 0.34023559336411324 1101 56-57 0.3350426567333429 1101 58-59 0.31247845323845524 1101 60-61 0.29685305890325253 1101 62-63 0.33328324564633505 1101 64-65 0.3056273372716447 1101 66-67 0.301192258715389 1101 68-69 0.29511101627238645 1101 70-71 0.2700157729495345 1101 72-73 0.29164369861531725 1101 74-75 0.2798623306966661 1101 76-77 0.32356896293054405 1101 78-79 0.30843556219765844 1101 80-81 0.2971781904997286 1101 82-83 0.25474138562066884 1101 84-85 0.34389196717410186 1101 86-87 0.37022139793787545 1101 88-89 0.42892263787994267 1101 90-91 0.38228775776017443 1101 92-93 0.390291278136047 1101 94-95 0.45870216517803186 1101 96-97 0.45327315583206484 1101 98-99 0.4149580734151499 1101 100 0.3886494436119463 1102 1 0.13510483501289627 1102 2 0.04453975933358123 1102 3 0.12200008919197813 1102 4 0.10446507239145575 1102 5 0.11685911643060365 1102 6 0.13128858954752332 1102 7 0.14872315037652584 1102 8 0.16025284937514073 1102 9 0.18955142135804692 1102 10-11 0.21451744500706837 1102 12-13 0.20834047534556532 1102 14-15 0.26962066576574983 1102 16-17 0.2664713590655765 1102 18-19 0.21997207236692162 1102 20-21 0.256672159104923 1102 22-23 0.23804819774382224 1102 24-25 0.26062805130375466 1102 26-27 0.28809052068827157 1102 28-29 0.28157865446276986 1102 30-31 0.2591297336293579 1102 32-33 0.33217842002231635 1102 34-35 0.30249505138921506 1102 36-37 0.3028679024243317 1102 38-39 0.30698449207193335 1102 40-41 0.3346439003977508 1102 42-43 0.289869907687077 1102 44-45 0.28517673005804056 1102 46-47 0.2539847231114791 1102 48-49 0.23853038385514225 1102 50-51 0.2711365108013055 1102 52-53 0.30626279959401614 1102 54-55 0.2990716588100568 1102 56-57 0.3193742795365253 1102 58-59 0.323908494666604 1102 60-61 0.3258987175438932 1102 62-63 0.3213285056577533 1102 64-65 0.3073479710312341 1102 66-67 0.29926082788564656 1102 68-69 0.3395788567271296 1102 70-71 0.30933397910283134 1102 72-73 0.3425245769461185 1102 74-75 0.3294493757415822 1102 76-77 0.2666287683439492 1102 78-79 0.3619158272937 1102 80-81 0.33056474254527757 1102 82-83 0.29713924496655153 1102 84-85 0.25292693357494045 1102 86-87 0.2683807142126966 1102 88-89 0.3447044180522809 1102 90-91 0.3146288637926844 1102 92-93 0.3534265863196069 1102 94-95 0.3566977315690991 1102 96-97 0.3400338350916883 1102 98-99 0.279014194170891 1102 100 0.2662802110169338 1103 1 0.07451729947779029 1103 2 0.07141322783596138 1103 3 0.07543107078929978 1103 4 0.0555142845149561 1103 5 0.06229029387068152 1103 6 0.08236568837145342 1103 7 0.10202287372592878 1103 8 0.08963918209418154 1103 9 0.16043875073140867 1103 10-11 0.1808661911774152 1103 12-13 0.13746261109983493 1103 14-15 0.19583041263081213 1103 16-17 0.17494461944414752 1103 18-19 0.19829874680431914 1103 20-21 0.18440822592836525 1103 22-23 0.18415224402230024 1103 24-25 0.18733766954481723 1103 26-27 0.17020210618616716 1103 28-29 0.19765283472094808 1103 30-31 0.17934638331571762 1103 32-33 0.16617195811973318 1103 34-35 0.1834123912123644 1103 36-37 0.1900624295094957 1103 38-39 0.19110837097206712 1103 40-41 0.2101231746749832 1103 42-43 0.17071214871681661 1103 44-45 0.11143659123132466 1103 46-47 0.0930051795958704 1103 48-49 0.017115705168066597 1103 50-51 0.019575014540841096 1103 52-53 0.011694175056916833 1103 54-55 0.060925323213325555 1103 56-57 0.03864558824575681 1103 58-59 0.04195110808826996 1103 60-61 0.07377544506594091 1103 62-63 0.03369971815332207 1103 64-65 0.025150708869361438 1103 66-67 -0.03980030048837335 1103 68-69 -0.029379743967297145 1103 70-71 -0.026734983526438327 1103 72-73 -0.0031813299112144477 1103 74-75 0.001178878925934157 1103 76-77 -0.017483524404276807 1103 78-79 0.005874764081909234 1103 80-81 0.0057590774211320195 1103 82-83 0.014440408250727899 1103 84-85 0.06309628214889074 1103 86-87 0.024381100993053906 1103 88-89 0.006667626319973863 1103 90-91 -0.0296933131648629 1103 92-93 0.05469094388269724 1103 94-95 0.05608080417843553 1103 96-97 0.01502853480187838 1103 98-99 0.033127573319561066 1103 100 0.0930351746679321 1104 1 0.09839983851590972 1104 2 0.037885989876052406 1104 3 0.05849296181122554 1104 4 0.04746422610277534 1104 5 0.0690759632770579 1104 6 0.033155150427006674 1104 7 0.06123459061602432 1104 8 0.07061500386936359 1104 9 0.12893436256795354 1104 10-11 0.13416138743130546 1104 12-13 0.1524188658294321 1104 14-15 0.1942729701868373 1104 16-17 0.21810097801761685 1104 18-19 0.1554408789732804 1104 20-21 0.18546077583418707 1104 22-23 0.1687601418879794 1104 24-25 0.16588047027780561 1104 26-27 0.1845601064112543 1104 28-29 0.1861400981377912 1104 30-31 0.15664844300108882 1104 32-33 0.14361579767126642 1104 34-35 0.1270719938714322 1104 36-37 0.14240009922243502 1104 38-39 0.18062971472154743 1104 40-41 0.17975770756394382 1104 42-43 0.16524711852338214 1104 44-45 0.1598914301904557 1104 46-47 0.11960583134740688 1104 48-49 0.05145745993632289 1104 50-51 0.11350705635043568 1104 52-53 0.1196795985935708 1104 54-55 0.13817068821352052 1104 56-57 0.1478400352218543 1104 58-59 0.16538753788869798 1104 60-61 0.2005469490001346 1104 62-63 0.1623899216155209 1104 64-65 0.11839368024739372 1104 66-67 0.12568629854168023 1104 68-69 0.18955652685671964 1104 70-71 0.18747113466639576 1104 72-73 0.1412078597267623 1104 74-75 0.07942028470556295 1104 76-77 0.13238674842066445 1104 78-79 0.05987783262024493 1104 80-81 0.05815181925861879 1104 82-83 0.09901724443700033 1104 84-85 0.09263868811632392 1104 86-87 0.1422961856630991 1104 88-89 0.1326083015330468 1104 90-91 0.1038687149116484 1104 92-93 0.11267888756673017 1104 94-95 0.10035895198058853 1104 96-97 0.1260924434952173 1104 98-99 0.11472567299034608 1104 100 0.11744880024170357 1105 1 0.0805787072711226 1105 2 0.10773571372546797 1105 3 0.09483317243914513 1105 4 0.04809236711562903 1105 5 0.03126857184745546 1105 6 0.042359718546201464 1105 7 0.0747765573794581 1105 8 0.0984005790332887 1105 9 0.11515847421996028 1105 10-11 0.08298039122082912 1105 12-13 0.15536818610254954 1105 14-15 0.16818554261018903 1105 16-17 0.17196394675338667 1105 18-19 0.16828612876459914 1105 20-21 0.15422651937241483 1105 22-23 0.17052255635270086 1105 24-25 0.1795631593418463 1105 26-27 0.1876098282908032 1105 28-29 0.21642734153197551 1105 30-31 0.18176127938649245 1105 32-33 0.2076114491921217 1105 34-35 0.2587559953586762 1105 36-37 0.2715595327874709 1105 38-39 0.2649075372317071 1105 40-41 0.1892446436590305 1105 42-43 0.23894411373907332 1105 44-45 0.3126156317175912 1105 46-47 0.32699375212924764 1105 48-49 0.33913927241000863 1105 50-51 0.30634209977366567 1105 52-53 0.29561448467375584 1105 54-55 0.2877966599114714 1105 56-57 0.27332043628656777 1105 58-59 0.2795323451988736 1105 60-61 0.2701955002868246 1105 62-63 0.2325942828987948 1105 64-65 0.24850468219510446 1105 66-67 0.2806677202432013 1105 68-69 0.3206135569390369 1105 70-71 0.3113625925888357 1105 72-73 0.25702138975121613 1105 74-75 0.25262579836042676 1105 76-77 0.3088171173519427 1105 78-79 0.27143743707115675 1105 80-81 0.26988280204938064 1105 82-83 0.299192931858677 1105 84-85 0.2530618030161378 1105 86-87 0.24187192577868188 1105 88-89 0.30941756187299774 1105 90-91 0.2440547195063587 1105 92-93 0.27324777254871435 1105 94-95 0.2337082904749721 1105 96-97 0.26723457178766097 1105 98-99 0.2471100233901069 1105 100 0.2396294911229475 1106 1 0.00465499679199155 1106 2 0.062076297326484564 1106 3 0.042721520200792895 1106 4 0.021893278344592204 1106 5 0.03489511869159401 1106 6 0.04757024006935495 1106 7 0.026803272527779143 1106 8 0.07141931543315394 1106 9 0.012314259205091105 1106 10-11 0.07270096232463885 1106 12-13 0.055473376214052905 1106 14-15 0.113730898388269 1106 16-17 0.09846274884211681 1106 18-19 0.11307921824526801 1106 20-21 0.09346093946089695 1106 22-23 0.07344778345344594 1106 24-25 0.08219142062780094 1106 26-27 0.1279130270121982 1106 28-29 0.09579854016543976 1106 30-31 0.08208477822940097 1106 32-33 0.07236475483063032 1106 34-35 9.954771325269007E-4 1106 36-37 0.078867655034081 1106 38-39 0.11348268222135971 1106 40-41 0.10521721258994887 1106 42-43 0.019478291105301082 1106 44-45 -0.04998465213871128 1106 46-47 0.04114920113402576 1106 48-49 0.0454961306482744 1106 50-51 0.04050423698553374 1106 52-53 -0.042205520915437944 1106 54-55 0.018968650453778935 1106 56-57 -0.027099068158108253 1106 58-59 -0.023971349015042165 1106 60-61 -0.010589170844930607 1106 62-63 -0.03636168169452958 1106 64-65 -0.005353832394924041 1106 66-67 -0.022130179722573473 1106 68-69 -0.04955005293439285 1106 70-71 -0.03549418557935269 1106 72-73 -0.039078333614490646 1106 74-75 0.005176423064092717 1106 76-77 -0.05256044920827918 1106 78-79 -0.009401320617925535 1106 80-81 -0.046502441818986995 1106 82-83 0.03987465798130074 1106 84-85 -0.022174011233822455 1106 86-87 0.016419608299230504 1106 88-89 0.0249131642983329 1106 90-91 0.004230135792305134 1106 92-93 -0.0598749450613667 1106 94-95 0.0046287981507120435 1106 96-97 0.033168911178066196 1106 98-99 0.05023589670354056 1106 100 0.05525838668957306 1107 1 0.025768505583251056 1107 2 -0.01499356266608487 1107 3 0.060128687961160665 1107 4 0.020077077701230905 1107 5 0.03774781201358479 1107 6 0.05948946045344172 1107 7 0.03513077333175829 1107 8 0.03395784798757262 1107 9 0.025103533606767314 1107 10-11 0.0697397720788615 1107 12-13 0.07307879305752607 1107 14-15 0.09729552603039338 1107 16-17 0.05227383042021927 1107 18-19 0.07376547112453835 1107 20-21 0.07705032503943698 1107 22-23 0.11305417160978237 1107 24-25 0.07539464327225431 1107 26-27 0.040211804749617386 1107 28-29 0.08456845898316345 1107 30-31 0.052620866615001205 1107 32-33 0.028985361094960638 1107 34-35 0.007090198102055467 1107 36-37 0.04977448145414343 1107 38-39 0.02253664843534864 1107 40-41 0.02209450112154343 1107 42-43 -0.003020325865151108 1107 44-45 0.016929888364380474 1107 46-47 0.004694643045745295 1107 48-49 -0.036646285316528804 1107 50-51 0.0017192406958841389 1107 52-53 -0.0030343453596302083 1107 54-55 0.04611348208050714 1107 56-57 -0.012436987636242236 1107 58-59 -0.02962395337178947 1107 60-61 0.008544185571068397 1107 62-63 0.00810349788253717 1107 64-65 -0.031949683692303665 1107 66-67 -0.04238781370420952 1107 68-69 0.009693461822521954 1107 70-71 0.05098034960245457 1107 72-73 8.120098846191581E-4 1107 74-75 0.011165616577464732 1107 76-77 0.09502053337075012 1107 78-79 0.10999314108737224 1107 80-81 0.05642368643766815 1107 82-83 0.10221024227008257 1107 84-85 0.09323316068380194 1107 86-87 0.06023302684836551 1107 88-89 0.1226087237861222 1107 90-91 0.10294893670938876 1107 92-93 0.12236284278026233 1107 94-95 0.09588376792599007 1107 96-97 0.10650277258172025 1107 98-99 0.18798838682486263 1107 100 0.18771854311518865 1108 1 -0.026008243737436487 1108 2 0.012916852985618732 1108 3 0.02857625031142419 1108 4 -0.03347915932945256 1108 5 -0.01864153038995653 1108 6 -0.016040328389891556 1108 7 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2214 56-57 -0.15567935192878934 2214 58-59 -0.1417416178407862 2214 60-61 -0.16645892993405909 2214 62-63 -0.18119681900883933 2214 64-65 -0.14234955506644553 2214 66-67 -0.1727645635795909 2214 68-69 -0.18630278028486202 2214 70-71 -0.12529301421338346 2214 72-73 -0.11624923179747526 2214 74-75 -0.17392493582632085 2214 76-77 -0.12262015517566738 2214 78-79 -0.10762863189219729 2214 80-81 -0.09921919186378148 2214 82-83 -0.1420823254204464 2214 84-85 -0.10002044786237718 2214 86-87 -0.11369251950243608 2214 88-89 -0.12672113353362846 2214 90-91 -0.07177127980337872 2214 92-93 -0.0783766940677566 2214 94-95 -0.10709751085534691 2214 96-97 -0.13038030176906545 2214 98-99 -0.14290001677052544 2214 100 -0.12106955506179418 2215 1 -0.16808879345725103 2215 2 -0.15948337600471518 2215 3 -0.18688840329004108 2215 4 -0.11790666933362814 2215 5 -0.12960307976611318 2215 6 -0.13493714077348073 2215 7 -0.16401331048459866 2215 8 -0.13006901294296114 2215 9 -0.16868272271572238 2215 10-11 -0.21432752227288177 2215 12-13 -0.19118964312807663 2215 14-15 -0.3045610149810045 2215 16-17 -0.2867683270549861 2215 18-19 -0.26901889978963567 2215 20-21 -0.22872077478287167 2215 22-23 -0.32635209255111164 2215 24-25 -0.3398758103824733 2215 26-27 -0.364438002672685 2215 28-29 -0.34843004914976206 2215 30-31 -0.33488701126307063 2215 32-33 -0.20790009913489627 2215 34-35 -0.20309829710753746 2215 36-37 -0.23653810194300462 2215 38-39 -0.23426539917966238 2215 40-41 -0.24185038605679665 2215 42-43 -0.20468483543838545 2215 44-45 -0.2550581930266276 2215 46-47 -0.2858022874106112 2215 48-49 -0.2508059994312646 2215 50-51 -0.2587985481665953 2215 52-53 -0.20329342767740144 2215 54-55 -0.27161033744733487 2215 56-57 -0.22076596146184357 2215 58-59 -0.15670025709270163 2215 60-61 -0.17539416039478084 2215 62-63 -0.16474790710351073 2215 64-65 -0.22655144558771667 2215 66-67 -0.21333979392917968 2215 68-69 -0.18182721611049146 2215 70-71 -0.23453427130105808 2215 72-73 -0.2096902648329788 2215 74-75 -0.20595856279209812 2215 76-77 -0.214591405311495 2215 78-79 -0.2388286152843051 2215 80-81 -0.20371766906219335 2215 82-83 -0.23826624577171884 2215 84-85 -0.21217919892428938 2215 86-87 -0.17873587288872272 2215 88-89 -0.20530400814343608 2215 90-91 -0.19005021612045425 2215 92-93 -0.189165988707785 2215 94-95 -0.17278025260203123 2215 96-97 -0.20514332291109838 2215 98-99 -0.25303317624579336 2215 100 -0.22618083410399237 2216 1 -0.1942166767967919 2216 2 -0.13849986148677118 2216 3 -0.1737373120303829 2216 4 -0.13247293205574096 2216 5 -0.10503868248633808 2216 6 -0.1457013866340091 2216 7 -0.1548940236789491 2216 8 -0.13139200904052473 2216 9 -0.16963385967257238 2216 10-11 -0.20977671314599888 2216 12-13 -0.26085239241405134 2216 14-15 -0.3344630263491908 2216 16-17 -0.2846917901181527 2216 18-19 -0.2762159301037883 2216 20-21 -0.29044202823574494 2216 22-23 -0.31741654181210066 2216 24-25 -0.3039760608299815 2216 26-27 -0.30971872643442566 2216 28-29 -0.31122885286587376 2216 30-31 -0.34587839322801983 2216 32-33 -0.29892863519606294 2216 34-35 -0.31604320142846376 2216 36-37 -0.29279408421216857 2216 38-39 -0.29172023969880456 2216 40-41 -0.24120754484181361 2216 42-43 -0.2485628762461829 2216 44-45 -0.3746549696753192 2216 46-47 -0.3290265315370604 2216 48-49 -0.30973216269751447 2216 50-51 -0.34493516116883427 2216 52-53 -0.360300313760483 2216 54-55 -0.39358964480104675 2216 56-57 -0.41445725676411627 2216 58-59 -0.37698613378033485 2216 60-61 -0.36052484946662133 2216 62-63 -0.42613811504742216 2216 64-65 -0.46271742140918803 2216 66-67 -0.44517743251975617 2216 68-69 -0.4189141742388429 2216 70-71 -0.40535618124066275 2216 72-73 -0.34303325922443406 2216 74-75 -0.40773463069757554 2216 76-77 -0.40065494812515823 2216 78-79 -0.37765894847100867 2216 80-81 -0.33386255914222573 2216 82-83 -0.31243586179696337 2216 84-85 -0.4282647690950867 2216 86-87 -0.4192485053127655 2216 88-89 -0.5500659417617939 2216 90-91 -0.48035176719995576 2216 92-93 -0.5256783115344561 2216 94-95 -0.4124357794757145 2216 96-97 -0.5436291362088781 2216 98-99 -0.621361330379397 2216 100 -0.6025290163245671 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 23.0 3 1.0 4 6.0 5 23.0 6 71.0 7 155.0 8 403.0 9 783.0 10 1479.0 11 2323.0 12 3678.0 13 5662.0 14 7190.0 15 9017.0 16 10982.0 17 15496.0 18 17894.0 19 9438.0 20 6471.0 21 6047.0 22 6073.0 23 6736.0 24 7647.0 25 9463.0 26 11950.0 27 15323.0 28 19891.0 29 25628.0 30 33340.0 31 42599.0 32 54791.0 33 71610.0 34 99408.0 35 150699.0 36 274023.0 37 636477.0 38 1238836.0 39 651094.0 40 2520.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.495221020214466 20.174678153590996 13.638724763772755 23.69137606242179 2 15.381830271739982 20.63501734022881 40.51869245913282 23.46445992889839 3 17.826665674913556 25.875581485204307 30.616199939742316 25.68155290013982 4 12.028976908483386 17.111483822046125 39.652288675070515 31.207250594399977 5 12.130882986676175 37.99604021603137 36.32614125969289 13.54693553759957 6 30.06129518381754 39.05920473992163 17.75108986419784 13.128410212062988 7 26.266116778814848 33.039027566746256 23.158092757398162 17.536762897040738 8 24.616090343941377 36.42212986652157 20.781798080596428 18.179981708940623 9 24.794993126401852 16.408827147095 21.749569495694956 37.04661023080819 10-11 23.336420440896845 26.816941274531654 29.54183947233614 20.304798812235365 12-13 24.533535923594528 24.216843933145213 29.64184935967007 21.607770783590187 14-15 21.56189286002668 26.175208820697392 27.185515783721925 25.077382535553998 16-17 20.614060474999466 29.627572103404503 27.600798901149155 22.15756852044688 18-19 20.40532994215009 28.193571232285393 30.43341920787142 20.967679617693097 20-21 21.704221359412685 27.496813182376556 30.010785038822814 20.78818041938795 22-23 21.19739920185156 27.355830133127963 30.14309830816726 21.303672356853216 24-25 21.095434279586883 27.432279705545692 30.12851625301538 21.343769761852048 26-27 20.889621559342615 27.39963843369116 30.20920806038473 21.501531946581494 28-29 20.918362474122613 27.463412784709124 30.23056016128561 21.387664579882653 30-31 21.23300338094596 27.916159949248186 29.75080500780552 21.100031662000333 32-33 20.8479704797048 27.589928369871934 30.33259532595326 21.22950582447001 34-35 21.014036610954346 27.346530641777004 30.251269806815717 21.388162940452933 36-37 21.382736415599453 27.75168222270458 29.624224006945955 21.24135735475002 38-39 21.259286592865926 27.953751537515377 29.68439331452138 21.102568555097314 40-41 21.070042170646555 27.624870233589665 29.855347533765315 21.44974006199846 42-43 21.11032486795456 27.55486578395196 29.704015628391577 21.630793719701902 44-45 21.30253961363143 27.837566022719052 29.496129078937848 21.36376528471167 46-47 21.38152087403227 28.14797771507127 29.107040011576586 21.363461399319874 48-49 21.373402792851458 27.982099703349977 28.94369437812025 21.700803125678316 50-51 21.383098183923018 27.927183271832718 28.861905795528543 21.827812748715722 52-53 21.413067071847188 28.001808841617827 28.830070183054772 21.755053903480214 54-55 21.572245134216047 28.396324433832575 28.566427899573117 21.465002532378268 56-57 21.330598364807177 28.021054916431513 28.752405759351713 21.895940959409593 58-59 21.156254974314447 27.67474133564865 29.157152159756894 22.01185153028001 60-61 21.491701034657407 28.11683669777874 28.639895810722816 21.75156645684104 62-63 21.27682512119239 28.065118298241803 28.751609869039868 21.90644671152594 64-65 21.327197742565662 27.754475074162503 28.94258013168367 21.97574705158816 66-67 21.200697663077587 27.87406524478189 28.74958812670402 22.175648965436505 68-69 21.715834415263053 28.005950951104495 28.569311707473215 21.708902926159237 70-71 21.664282130711545 27.8047648946914 28.609091760233234 21.921861214363826 72-73 21.399336371226934 27.94798921639421 28.77088310669721 21.881791305681652 74-75 21.49919795904635 28.042144593115253 28.56131145453401 21.897345993304388 76-77 21.528586932928153 28.06260039070979 28.40829172997612 22.000520946385933 78-79 21.695116127631863 28.14679111496997 28.467144200853774 21.69094855654439 80-81 21.565747775124812 27.949526083496128 28.576514000434123 21.90821214094494 82-83 21.399922465779234 27.96166469647552 28.75493615003378 21.88347668771146 84-85 21.3814629911005 27.91530280008682 28.56773026553795 22.135503943274728 86-87 21.52157413202421 28.07492972624892 28.43535357555016 21.96814256617671 88-89 21.25922094851319 27.96817007295721 28.563103587197876 22.20950539133173 90-91 21.21489745615316 27.842944818657084 28.580084311080128 22.362073414109624 92-93 21.364724173449375 28.044114535835934 28.36685688056348 22.22430441015121 94-95 21.131083048717063 27.786270522108904 28.612870839250682 22.469775589923355 96-97 21.097484810054045 27.845224334244218 28.58252350194458 22.474767353757155 98-99 21.076388728102874 27.905449912712488 28.527112959764395 22.491048399420244 100 21.28335142174951 27.827393097460384 28.366977787424936 22.52227769336517 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 20.0 1 23.5 2 102.5 3 191.5 4 139.5 5 105.0 6 260.0 7 575.0 8 993.5 9 1199.5 10 1047.0 11 925.5 12 1028.0 13 1266.5 14 1553.0 15 1774.0 16 1998.5 17 2253.5 18 2692.0 19 3292.0 20 4088.0 21 5204.5 22 6795.5 23 9588.0 24 13108.0 25 16941.5 26 22226.0 27 29422.5 28 38775.0 29 49197.5 30 59060.0 31 69587.5 32 80608.5 33 91821.5 34 103190.0 35 111990.0 36 118324.0 37 124273.0 38 128083.0 39 129972.0 40 131405.0 41 130925.0 42 128868.5 43 125949.5 44 122529.0 45 119092.5 46 116470.5 47 112677.5 48 108419.5 49 105146.0 50 101628.0 51 97595.5 52 91548.5 53 86487.0 54 82411.0 55 77974.5 56 72185.5 57 65226.5 58 59328.0 59 53610.5 60 47102.0 61 40049.5 62 33497.0 63 27239.5 64 21606.0 65 16837.5 66 12818.0 67 9447.5 68 6976.5 69 5130.5 70 3495.5 71 2402.5 72 1763.0 73 1255.0 74 872.0 75 598.0 76 377.5 77 237.5 78 162.5 79 106.0 80 61.0 81 21.5 82 10.5 83 5.0 84 3.5 85 3.0 86 1.5 87 1.0 88 1.0 89 1.0 90 0.5 91 0.5 92 0.5 93 0.5 94 1.0 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.045843281962231384 2 0.24171912307358367 3 0.0025757904637869907 4 0.0024600246002460025 5 3.18356124737718E-4 6 8.682439765574127E-5 7 0.0 8 2.894146588524709E-4 9 0.0 10-11 5.788293177049418E-4 12-13 0.0 14-15 3.762390565082121E-4 16-17 1.1576586354098836E-4 18-19 4.485927212213299E-4 20-21 1.4470732942623545E-5 22-23 8.682439765574127E-5 24-25 0.001302365964836119 26-27 1.59178062368859E-4 28-29 3.762390565082121E-4 30-31 1.1576586354098836E-4 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 8.682439765574127E-5 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 1.4470732942623545E-5 68-69 1.1576586354098836E-4 70-71 1.4470732942623545E-5 72-73 1.4470732942623544E-4 74-75 7.235366471311772E-5 76-77 0.0 78-79 0.0 80-81 0.0 82-83 4.3412198827870635E-5 84-85 0.0 86-87 3.617683235655886E-4 88-89 0.0021416684755082844 90-91 0.0020114318790246727 92-93 4.920049200492005E-4 94-95 3.762390565082121E-4 96-97 1.1576586354098836E-4 98-99 5.788293177049418E-5 100 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 3455250.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.03265118361382 #Duplication Level Percentage of deduplicated Percentage of total 1 77.387831184674 51.1012366247397 2 14.19847044478708 18.751252924429508 3 4.206064338757884 8.332127380111103 4 1.696002670215688 4.479662109153206 5 0.7949951453810012 2.6247818563805003 6 0.4607638759461482 1.8255276179017148 7 0.28067634675592007 1.2973662310577292 8 0.19159957856405233 1.0121462510597983 9 0.13666263008575888 0.8121776204059347 >10 0.6089737568866642 6.871365362171936 >50 0.027495452189341938 1.248173693547029 >100 0.010026150100972664 1.1399241168343273 >500 2.6305539364106207E-4 0.11174857646469293 >1k 1.3152769629228697E-4 0.23536681948585308 >5k 4.384256543076232E-5 0.1571428162569755 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5428 0.1570942768251212 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4496 0.1301208306200709 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 2.8941465885247088E-5 0.0 4 0.0 0.0 0.0 2.8941465885247088E-5 0.0 5 0.0 0.0 0.0 2.8941465885247088E-5 0.0 6 0.0 0.0 0.0 2.8941465885247088E-5 0.0 7 0.0 0.0 0.0 8.682439765574127E-5 0.0 8 0.0 0.0 0.0 8.682439765574127E-5 0.0 9 0.0 0.0 0.0 8.682439765574127E-5 0.0 10-11 0.0 0.0 0.0 8.682439765574127E-5 0.0 12-13 0.0 0.0 0.0 8.682439765574127E-5 0.0 14-15 0.0 0.0 0.0 1.4470732942623544E-4 0.0 16-17 0.0 0.0 0.0 1.4470732942623544E-4 0.0 18-19 0.0 0.0 0.0 1.4470732942623544E-4 0.0 20-21 0.0 0.0 0.0 1.59178062368859E-4 0.0 22-23 0.0 0.0 0.0 1.7364879531148254E-4 0.0 24-25 0.0 0.0 0.0 2.604731929672238E-4 0.0 26-27 0.0 0.0 0.0 3.907097894508357E-4 0.0 28-29 0.0 0.0 0.0 9.405976412705304E-4 0.0 30-31 0.0 0.0 0.0 0.002850734389696838 0.0 32-33 0.0 0.0 0.0 0.008566673902033138 0.0 34-35 0.0 0.0 0.0 0.017162289269951524 0.0 36-37 0.0 0.0 0.0 0.0318356124737718 0.0 38-39 0.0 0.0 0.0 0.05805658056580566 0.0 40-41 0.0 0.0 0.0 0.09241010057159395 0.0 42-43 0.0 0.0 0.0 0.12690832790680848 0.0 44-45 0.0 0.0 0.0 0.1673106142826134 0.0 46-47 0.0 0.0 0.0 0.21630851602633672 0.0 48-49 0.0 0.0 0.0 0.27255625497431446 0.0 50-51 0.0 0.0 0.0 0.3347369944287678 0.0 52-53 0.0 0.0 0.0 0.3985818681716229 0.0 54-55 0.0 0.0 0.0 0.46822950582447 0.0 56-57 0.0 0.0 0.0 0.5501772664785471 0.0 58-59 2.8941465885247088E-5 0.0 0.0 0.6452210404456986 0.0 60-61 2.8941465885247088E-5 0.0 0.0 0.7460386368569568 0.0 62-63 2.8941465885247088E-5 0.0 0.0 0.8530497069676579 0.0 64-65 2.8941465885247088E-5 0.0 0.0 0.9659214239201215 0.0 66-67 2.8941465885247088E-5 0.0 0.0 1.099630996309963 0.0 68-69 2.8941465885247088E-5 0.0 0.0 1.2505317994356413 0.0 70-71 2.8941465885247088E-5 0.0 0.0 1.41522321105564 0.0 72-73 2.8941465885247088E-5 0.0 0.0 1.5948773605383113 0.0 74-75 2.8941465885247088E-5 0.0 0.0 1.777671659069532 0.0 76-77 2.8941465885247088E-5 0.0 0.0 1.9679328557991465 0.0 78-79 2.8941465885247088E-5 0.0 0.0 2.1892048332248026 0.0 80-81 2.8941465885247088E-5 0.0 0.0 2.434671876130526 0.0 82-83 2.8941465885247088E-5 0.0 0.0 2.6929165762245857 0.0 84-85 2.8941465885247088E-5 0.0 0.0 2.958338759858187 0.0 86-87 2.8941465885247088E-5 0.0 0.0 3.231777729542001 0.0 88 2.8941465885247088E-5 0.0 0.0 3.454192894870125 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4585 0.0 51.713154 1 GTATCAA 8565 0.0 41.99229 1 ATCAACG 10045 0.0 35.04453 3 TCAACGC 10045 0.0 34.95095 4 CAACGCA 10255 0.0 34.143574 5 TATCAAC 10875 0.0 32.536346 2 AACGCAG 10820 0.0 32.447063 6 ACGCAGA 12505 0.0 27.962198 7 CGCAGAG 12670 0.0 27.450071 8 GTACATG 8150 0.0 26.66341 1 TACATGG 8080 0.0 25.073511 2 TATACCG 195 1.7595085E-8 24.102037 5 GCAGAGT 14315 0.0 24.09867 9 TAGACCG 145 2.5356023E-5 22.68916 5 ACATGGG 8625 0.0 22.559507 3 GAGTACT 9760 0.0 21.380062 12-13 AGAGTAC 12745 0.0 20.79829 10-11 GTGGTAT 2535 0.0 20.640368 1 CAGAGTA 13925 0.0 20.149649 10-11 CATGGGG 6025 0.0 19.891682 4 >>END_MODULE