FastQCFastQC Report
Wed 25 May 2016
SRR1033076_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033076_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2510820
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC29800.032.182374
GTATCAA31750.031.8402771
ATCAACG30850.031.0870253
CAACGCA31200.030.738295
GGTATCA19950.030.6395591
AACGCAG34750.027.589876
TATCAAC38050.026.5636632
GTACATG65000.025.6077821
CTGTCGC6800.024.2222149
TACATGG66000.023.5057542
TACACCG3950.022.6131295
ACGCAGA41050.022.4397077
CGCAGAG41500.022.1060528
GTCTCGC5850.021.7015131
ACATGGG70950.021.5345173
GAGTACA49750.020.3201771
CCTACAC10250.019.72193
CATGGGG45950.018.4158234
CTACACT13150.018.23264
AGTACAT51050.017.4048462
Produced by FastQC (version 0.11.5)